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Citations for Addgene :
1201 - 1250 of 1882 citations for Vascular Endothelial Cell Growth Factor VEGF Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: XEN cells were infected with lentiviruses harboring the pHR–SFFV–dCas9–BFP–KRAB vector (Addgene, cat. no. 46911), while ESC v6,5 cells were infected with a modified version of the plasmid in which the SFFV promoter was replaced with an Ef1a promoter 42 ...
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bioRxiv - Cancer Biology 2023Quote: ... Lung KP cells stably expressing a hypoxia reporter (pLenti-5XHRE-GFP, Addgene #128958, denoted here as HRE-GFP), cell membrane marker (pLenti-mCherry-CAAX ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... RBL-2H3 cells were transfected with the mitochondrial calcium indicator pCMV CEPIA2mt (a gift from Masamitsu Iino; Addgene plasmid # 58218 ...
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bioRxiv - Synthetic Biology 2023Quote: Reporter plasmid was co-transformed with accessory and selection plasmids of interest into electrocompetent S1030 cells (Addgene #105063) and recovered using Davis rich media60 (DRM) ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells (between passage 9-11) were transfected with lentiviral packaging vector psPAX2 (#12259, Addgene, Watertown, MA, USA), the envelope vector pMD2.G (#12260 ...
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bioRxiv - Genomics 2023Quote: AAV production was performed by transfecting 293FT cells with 22 ug of pDGM6 helper plasmid (Addgene plasmid # 110660), 6 ug of donor template plasmid ...
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bioRxiv - Genetics 2023Quote: ... 293T cells were transfected with 7.5 μg of of the plasmid pCMVR8.74 (a gift from Didier Trono (Addgene plasmid #22036 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were transfected using 30 μg of pMSCV-loxP-dsRed-loxP-eGFP-Puro-WPRE plasmid (Addgene, Plasmid #32702) or pMSCV-loxP-dsRed-loxP-Cited2-3HA-Puro-WPRE plasmid (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: KEAP1-sg1 knockout H1299 (4 x 105) cells were transfected with or without plasmid expressing Halo-KEAP1 (Addgene, a gift from Yimon Aye ...
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bioRxiv - Cancer Biology 2023Quote: ... Lentivirus was produced in 293 cells by co-transfecting the lentiviral constructs with pCMV-dR8.2 (Addgene plasmid #8455) and pCMV-VSV-G (Addgene plasmid #8454 ...
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bioRxiv - Immunology 2023Quote: ... DC 2.4 cells were transduced using the lentiCas9-Blast vector (Addgene plasmid # 52962; http://n2t.net/addgene:52962; RRID: Addgene_52962) and selected with 10 ug/mL of Blasticidin (AG Scientific #3513-03-9) ...
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bioRxiv - Microbiology 2024Quote: ... N/Tert-1 Cas9 knockout cells were generated by transduction with a spCas9 lentiviral expression vector (Addgene # 52962) and selecting with blasticidin ...
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bioRxiv - Bioengineering 2023Quote: ... Lentiviruses were packaged using 293FT cells by co-transfection of lentiviral transfer plasmids with pMD2.G (Addgene #12259) and psPAX2 (Addgene #12260 ...
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bioRxiv - Cancer Biology 2023Quote: ... MCF7 CRISPRi were prepared by transduction of MCF7 cells with lentiviral particles produced using vector pMH0001 (Addgene #85969) in the presence of 8 mg/mL polybrene (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... MCF7 cells were first transduced with lentiviral particles produced using vector pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP (Addgene #60903) in the presence of 8 mg/mL polybrene ...
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bioRxiv - Cancer Biology 2023Quote: ... Ifngr2−/− and Etv4−/− KPAR cell lines were generated by transient transfection of a Cas9-sgRNA plasmid (pX459, Addgene) generated by standard molecular cloning techniques (see Supplementary Table 3) ...
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bioRxiv - Biophysics 2023Quote: ... 37] was used to generate the α-catenin DVBS in α-catenin KO cell line (Addgene plasmid 178649).
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bioRxiv - Microbiology 2023Quote: ... we rescued lentiviral particles by co-transfecting 293T cells with the MKRN2 lentiviral vector alongside d8.74 (Addgene; 22036) and MD2G (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... The plasmid encoding NPM1-mEGFP was a gift from the Allen Institute for Cell Science (Addgene plasmid # 109122). The sgRNA was synthesized by Synthego with modifications using the protospacer sequence UCCAGGCUAUUCAAGAUCUC (Wienert ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK293T cells were co-transfected with lenti-EF1a-dCas9-KRAB-Puro plasmid (a gift from Kristen Brennand; Addgene_99372) or pLV-dCas9-p300-P2A-PuroR plasmid (a gift from Charles Gersbach ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells (2.5 x 105) were plated in a 6-well plate and transfected with pLenti-BLRR (Addgene # 158958), pLenti-trGluc (Addgene # 158959) ...
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bioRxiv - Neuroscience 2024Quote: ... 6 × 107 RPE1 cells were infected with the human sgRNA library (Human GeCKO v2 Library Cat#1000000048, Addgene) at an MOI of ∼0.3 ...
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bioRxiv - Biochemistry 2024Quote: ... cells were transfected 24h after seeding with mammalian expression plasmids to visualize Golgi (EYFP-Golgi7, Addgene plasmids # 56590), using JetPRIME transfection reagent (Polyplus transfection ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were transfected with a mixture of 1.7 μg attB plasmid + 0.2 μg pCAG-NLS-Bxb1 (Addgene#51271) + 0.2 μg pMAX-GFP + 7.8 μL Fugene 6 (for LLP lines ...
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bioRxiv - Microbiology 2024Quote: ... Approximately 200 million K562-Cas9-Blast cells were transduced with each GeCKOv2 library A or B [46] (Addgene #1000000048 or #1000000049 ...
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bioRxiv - Cancer Biology 2024Quote: ... 293T cells were plated in 10cm plates and transfected using lipofectamine 2000 with 6μg lentiviral pCW57.1-4EBP1_4xAla (Addgene: 38240), 1.5μg pMD2G and 4.5μg psPax2 ...
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bioRxiv - Molecular Biology 2024Quote: Non-replicating lentiviruses were produced in HEK293T cells using the 2nd generation packaging plasmids psPAX2 construct (Addgene #12260), pMD2.G construct (Addgene #12259) ...
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bioRxiv - Molecular Biology 2019Quote: ... dCas9-expressing cells were then transduced with the Calabrese Human CRISPR Activation Pooled Library (Set A, Addgene, 92379-LV) using enough cells to obtain a library coverage of 500 cells per sgRNA at an MOI of 0.4.17 Selection with puromycin (0.6 μg/ml ...
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bioRxiv - Cancer Biology 2021Quote: SPRY2 CRISPR oligonucleotides used in H1299 cells (gRNA target site: GTACTCATTGGTGTTTCGGA) were cloned into pSpCas9(BB)-2A-Puro (Addgene plasmid #62988 ...
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bioRxiv - Cell Biology 2020Quote: ... a cell line stably expressing Cas9 was generated by infection with lentiCas9-Blast (Addgene 52962, gift from Feng Zhang) and selection with blasticidin ...
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bioRxiv - Immunology 2022Quote: ... To subclone the fusion protein constructs GFPNKG2D-S/L into the retroviral stem cell vector pMIGR1 (Addgene, plasmid 27490), forward 5’ TAGTAGGAATTCGCCACCATGAGCGGGGGCGAGGAC 3’ and the reverse 5’ TAGAGGTCGACCTTACACCGCCCTTTTCATGCAG3’ primers were used ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 cells were transfected with 4µg pSMPUW-IRIS-Neo-H2BmRFP (Fachinetti Lab) + 4µg pMD2.G (12259 from Addgene, RRID:Addgene_12259) + 4µg psPAX2 (12260 from Addgene ...
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bioRxiv - Microbiology 2022Quote: ... ACE2-expressing lentivectors were generated via transient co-transfection of HEK293T cells with RRL.sin.cPPT.SFFV/Ace2.IRES-puro (Addgene Plasmid #145839), psPAX2 and VSV-G ...
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bioRxiv - Molecular Biology 2020Quote: ... this line (148.4) was derived from E14 mouse ES cells and is homozygous for a Tir1-2A-Puro cassette (Addgene plasmid # 92142 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected with the addressing and packaging plasmids (pCMV delta R8.2, Addgene #12263; pCMV-VSV-G, Addgene #8454). After 48 hours of expression ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were transfected with the addressing and packaging plasmids (pCMV delta R8.2, Addgene #12263; pCMV-VSV-G, Addgene #8454). After 48 hours of expression ...
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bioRxiv - Molecular Biology 2020Quote: ... These parental BV2 or BV2-Cas9 cells were transduced for 2 d with pXPR_011 lentivirus expressing eGFP (Addgene; 59702) and an sgRNA targeting eGFP at a multiplicity of infection (MOI ...
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bioRxiv - Molecular Biology 2021Quote: ... The IRE-SunTag reporter was integrated in previously established HeLa cell line stably expressing scFv-GFP (Addgene plasmid: 104998) and NLS-stdMCP-stdHalo fusion protein (Addgene plasmid ...
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bioRxiv - Genomics 2020Quote: ... Mutagenesis was performed via either an inducible Cas9-expressing OCI-AML3 cell line (Lenti-iCas9-neo vector; Addgene 85400) with lentiviral sgRNA expression (Addgene 70683) ...
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bioRxiv - Genomics 2020Quote: ... Cas9 expressing human melanoma cell line 2686 was transduced with lentiviral particles produced as described above using expression plasmid pXPR_011 (Addgene #59702 ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293T/17 cells were cotransfected with the respective transfer vector and second-generation lentiviral cassettes (packaging vector psPAX2 (Addgene RRID:Addgene_12260) and envelope vector pMD2.G (Addgene RRID:Addgene_12259) ...
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bioRxiv - Cell Biology 2019Quote: ... Lentivirus carrying CRISPR-Cas9 constructs were produced using HEK293 cells with the following plasmids: pSS172 (pMD2.G, Addgene #12259), pSS173 (psPAX2 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... we co-transfected cells with a plasmid for GCaMP6s under a ubiquitous promoter for Ca2+-imaging (Addgene, plasmid #40753) and a plasmid encoding for the mGlu5 receptor lacking eYFP16 to avoid bleed-through into the Ca2+-reporter channel.
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bioRxiv - Biochemistry 2019Quote: ... 4T1 and E0771 cGAS−/− cells were created by viral transfection of CRISPR sgRNA (using lentiCRISPRv2-blast, Addgene plasmid #83480) targeting mouse Mb21d1 (5’-CACCGGAAGGGGCGCGCGCTCCACC-3’) ...
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bioRxiv - Biophysics 2019Quote: ... virus was produced in HEK 293T cells by co-transfecting the lentiviral plasmids pLenti.PGK.LifeAct-Ruby.W (a gift from Rusty Lansford - Addgene plasmid #51009) with psPAX2 and pMD2.G (a gift from Didier Trono - Addgene plasmid #12260 and #12259) ...
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bioRxiv - Microbiology 2020Quote: The mouse cDNA sequence of filamin A from MEF cells was amplified and cloned into pcDNA3-myc plasmid (Addgene). The resulting plasmid pcDNA3- myc-FLNA was transiently transfected in the MEF cells and then the transfected cells were infected with Toxoplasma for 18 h (MOI ...
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bioRxiv - Cancer Biology 2019Quote: ... K562 cells (ATCC CCL-243) were first transduced with lentiviral particles produced using vector pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP (Addgene #60903 ...
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bioRxiv - Cell Biology 2020Quote: ... 400,000 cells were transiently co-transfected with 200 ng of each gRNA expression plasmid (cloned into Addgene plasmid #43860), 500 ng Cas9 expression plasmid (Addgene plasmid #43945) ...
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bioRxiv - Cancer Biology 2020Quote: Human ATG5 knockout P3 and T98G GBM cell lines were generated by using LentiCRISPRv268 (purchased from Addgene, plasmid# 99573). Plasmids were transfected into 293T cells using BBS/CaCl2 to produce lentivirus ...
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bioRxiv - Molecular Biology 2021Quote: ... stable Cas9 expression was induced by transducing FL83B cells with lentiCas9-Blast (Addgene, #52962-LV, gift from Feng Zhang) lentivirus at a multiplicity of infection (MOI ...