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Citations for Addgene :
1201 - 1250 of 2354 citations for Primary Human Aortic Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... 200000 cells were nucleofected using Amaxa 4D nucleofector with Addgene plasmids pCXLE-hUL (Addgene plasmid #27080 ...
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bioRxiv - Cancer Biology 2021Quote: ... A549-Cas9 cell line was generated by lentiviral transduction with lentiCas9-Blast (Addgene #52962) and selection with 100 μg/mL of blasticidin (Sigma ...
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bioRxiv - Systems Biology 2020Quote: ... Yeast competent cells were co-transformed with a pCAS plasmid (Addgene plasmid 60847, (46)) expressing both the gRNA of interest and Streptococcus pyogenes Cas9 and a donor DNA sequence (stuffer ...
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bioRxiv - Molecular Biology 2021Quote: ... 2×106 cells were co-transfected with Cas9 (pU6_CBh-Cas9-T2A-BFP: Addgene #64323) and gRNA (pSPgRNA ...
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bioRxiv - Cell Biology 2021Quote: To determine the cytosolic volume of all cell lines pEGFP-N1 (Addgene# 6085-1) was transiently overexpressed ...
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bioRxiv - Cell Biology 2020Quote: ... Lentiviral particles were produced in HEK-293T cells with the vectors psPAX2 (12259, Addgene), pMD2.G (12259 ...
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bioRxiv - Cell Biology 2020Quote: Coilin-KO cells were generated using the Cas9 nickase system (pX335 and pKN7; Addgene) [Cong et al ...
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bioRxiv - Cell Biology 2022Quote: Cas9-expressing J774 cells were transduced with lentivirus based on pMCB320 (Addgene plasmid #89359) with mCherry replaced by BFP and containing the sgRNA sequences given in the table below ...
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bioRxiv - Neuroscience 2022Quote: ... cells were co-transfected with 750 ng of pCMV-PE2 Plasmid (Addgene Plasmid #132775) and 250 ng of assembled pU6-pegRNA-GG-acceptor using Lipofectamine 2000 (Invitrogen) ...
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Activation of the Integrated Stress Response overcomes multidrug resistance in FBXW7-deficient cellsbioRxiv - Cancer Biology 2022Quote: ... Each cell line was also infected with the empty pLentiCRISPR v2 vector (Addgene, 52961) to be used as controls ...
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bioRxiv - Microbiology 2022Quote: ... coli DH10Bac cells following transformation with the pFastBac LIC cloning vector (4A) (Addgene #30111) containing a cloned copy of the AAV4 VP1 gene ...
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bioRxiv - Genetics 2022Quote: ... HEK- 293T cells were transfected with 100 ng pIS0 (Addgene #12178, encoding firefly luciferase), 100 ng of the Renilla reporter and 800 ng of empty vector (1 µg total plasmid DNA ...
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bioRxiv - Microbiology 2022Quote: MGAT1 and C1GALT1 KO A549 cells were generated using the lentiCRISPR v2 (#52961, Addgene) single vector system as previously described with Puromycin selection (Sanjana et al. ...
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bioRxiv - Genomics 2022Quote: A549 cells expanded from a single clone harboring a doxycycline-inducible Cas9 (Addgene #114010) were a gift from J.T ...
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bioRxiv - Cell Biology 2022Quote: ... Lentivirus was generated in 293T cells by calcium phosphate cotransfection with psPAX2 (Addgene #12260), pCMV-VSV-g (Addgene #8454) ...
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Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... unlabeled HeLa-TDS cells were transfected with CENP-B-INCENP-GFP (Addgene, plasmid #45238), and for live-cell imaging also with mCherry-PRC1 plasmid provided by Casper C ...
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bioRxiv - Cancer Biology 2022Quote: ... RPE1 hTERT cells were retrovirally infected with pBABE c-Myc-ER plasmid (Addgene, 19128), infected cells were selected in 5μg/ml puromycin and the surviving polyclonal population was used in further assays ...
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bioRxiv - Biophysics 2022Quote: ... These cells were infected with a lentiviral vector expressing pCMV-CheRiff-CFP (Addgene 136636) to render them light sensitive.
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bioRxiv - Developmental Biology 2021Quote: ... and 8×105 cells were electroporated with 2 μg Cas9-sgRNA plasmid (Addgene: #48139), 1 µg pEGFP-Puro and 200μM (6.6 μg ...
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bioRxiv - Molecular Biology 2020Quote: ... HCT116-Cas9 cell line was generated by lentiviral transduction with lentiCas9-Blast (Addgene # 52962) and selection with 4 μg/mL of blasticidin (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... Cas9-expressing HAP1 cell line was established by using lentiCas9-Blast plasmid (#52962, Addgene). To generate NSUN2-KO ...
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bioRxiv - Biophysics 2021Quote: ... for the live-cell time-lapse experiment and with pcDNA3.1(+)eGFP (Addgene plasmid #129020) for the FFS measurements ...
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bioRxiv - Cancer Biology 2021Quote: A549 cells were stably transduced with a lentiviral vector containing H2B-GFP (Addgene #25999) and a lentiviral vector containing DHB-iRFP ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were then transduced with the following virus: pTet-O-NGN2-puro (Addgene #52047): 0.1 μL per 5×104 cells ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were transiently transfected for 48 hours with XBP1s plasmid (Addgene, Teddington, United Kingdom) using Lipofectamine 2000 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2019Quote: ... Lentivirus were generated using co-transfection into HEK-293t cells of psPAX2 (Addgene, 12260), pMD2.G (AddGene ...
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bioRxiv - Developmental Biology 2020Quote: Lentiviral transfer vectors were transfected into 293T cells with packaging vector pspax2 (Addgene 12260) and envelop vector vsvg using linear polyethylenimine ...
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bioRxiv - Molecular Biology 2021Quote: ... lentiviruses were produced in HEK293T cells in a pLKO.1-puro (Addgene, plasmid 8453) backbone ...
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bioRxiv - Genomics 2021Quote: ... IMR90 cells were transfected with 1 µg of pEGFP-Δ50 LMNA (Addgene Plasmid #17653). After expression of the GFP was observed (∼2 days) ...
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bioRxiv - Bioengineering 2020Quote: ... U87-EGFP cells were created by infection with lentivirus made from pLL3.7 (Addgene #11795). The drug screens were performed by the Quellos High Throughput Screening Core (University of Washington ...
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bioRxiv - Neuroscience 2021Quote: ... 1 million cells were nucleofected with iμg pAAV-CAG-GFP plasmid DNA (Addgene 37825) in Ingenio electroporation buffer (Mirus MIR50111 ...
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bioRxiv - Genomics 2019Quote: ... the 70-80% confluent cells were transfected with 780 ng psPAX2 (Addgene plasmid #12260), 510 ng pMD2.G (Addgene plasmid #12259 ...
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bioRxiv - Cell Biology 2021Quote: Lentivirus was generated in 293T cells using delta 8.9 and pHCMV-EcoEnv (Addgene 15802) as packaging plasmids and 25kDa linear PEI as a transfection agent ...
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bioRxiv - Cell Biology 2019Quote: ... cells expressing GFP-tagged endogenous CLIC4 were transfected with Cry2PHR-mCH-RhoA (Addgene #42959). Cells were kept in the dark and incubated for 24 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... 293T cells were transfected with lentiviral packaging vectors (3 μg pSPAX2 (Addgene plasmid, 12260) and 1 μg pMD2.G (Addgene plasmid ...
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bioRxiv - Molecular Biology 2022Quote: ... HeLa cells (ATCCCCL-2) were transiently transfected with SpCas9-expressing PX459 plasmid (Addgene # 62988) using Lipofectamine 2000 (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2022Quote: Parental Cas9 cell lines were generated by viral infection with lentiCas9-Blast (Addgene, # 52962) followed by blasticidin selection for 7 days ...
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bioRxiv - Cell Biology 2022Quote: For transient cell transfections the following plasmids were used: talin-mCherry (Addgene plasmid # 55137), vinculin-mCherry14 ...
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bioRxiv - Cell Biology 2022Quote: ... RAW264.7 cells seeded onto coverslips were transfected with GCpGP-CMV-GCaMP6s (Addgene plasmid 40753) using FuGENE HD (Promega) ...
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bioRxiv - Developmental Biology 2022Quote: Lentiviral transfer vectors were transfected into 293T cells with packaging vector pspax2 (Addgene, 12260) and envelop vector vsvg using linear polyethylenimine ...
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bioRxiv - Molecular Biology 2023Quote: Mitochondrial immunoprecipitations were performed using K562 cells expressing an HA-mito tag (Addgene #83356) or a control MYC-mito tag (Addgene #83355 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were co-transfected with 750 ng of pCMV-PE2 Plasmid (Addgene Plasmid #132775) and 250 ng of assembled pU6-pegRNA-GG-acceptor using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: HEK293T cells (∼107) were transiently transfected with either pRK5-MYC-mTOR (Addgene plasmid #1861) or pRK5-MYC-mTOR kinase-dead (Addgene plasmid #8482) ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA from RPE1 cells was cloned into pLenti CMVie-IRES-BlastR (Addgene plasmid #119863). PAXIP1 W75R ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were transfected with 0.24μg EGFP-LC3 plasmid DNA (Addgene #11546; Watertown, MA, USA) using the FUGENE6 transfection reagent (Promega ...
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bioRxiv - Biophysics 2023Quote: ... Cells were transfected with paxillin-pEGFP (a gift from Rick Horwitz; Addgene plasmid #15233) using a microporator (Neon Transfection System ...
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bioRxiv - Biochemistry 2023Quote: ... and TEV protease cleavage site into an insect cell expression vector (Addgene plasmid #55218). The same expression construct was cloned into a bacterial expression vector (Addgene plasmid #29708) ...
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bioRxiv - Cancer Biology 2023Quote: ... KMS11 or JJN3 cells were transduced with the pCW-Cas9-Blast vector (#83481, Addgene) to establish stable Cas9+KMS11 and Cas9+JJN3 cells ...
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bioRxiv - Cancer Biology 2023Quote: VUMC-ATRT-01 cells were transduced (lentiviral) with a LentiCRISPR v2 plasmid (#52961, Addgene) that was cloned with a p53-sgRNA (Supplementary table S2 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysosome/autophagosome fusion was estimated in ARPE19 cells overexpressing only GFP-LC3 (24920, Addgene) or both GFP-LC3 and AKT2 constructs ...