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Citations for Addgene :
1101 - 1150 of 10000+ citations for Rat FAM24A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... SKO cells were transfected with pSH-EFIRES-P-GFP(1-10)opti AAVS1 Safe Harbor integration plasmid (Addgene plasmid # 129416, [35] and pCas9-sgAAVS1-2 (Addgene plasmid # 129727 [47]), and a stable pool was selected using puromycin (1 µg/ml puromycin for 6 days ...
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bioRxiv - Cell Biology 2020Quote: ... the Mouse GeCKOv2 CRISPR knockout pooled library plasmids were co-transfected with packaging plasmids pCMV-VSV-G and pCMV-dR8.2 dvpr (Gifts from Bob Weinberg, Addgene plasmid #8485 and #8455) into HEK293T cells ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293T cells were transfected with above lentiCRISPR SHMT2 plasmid or control V2 plasmid together with helper plasmids pPAX2 and UVSVG (Addgene #8454 and #12260) using Lipofectamine 2000 (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... the retroviral FLAG-5SA-YAP and FLAG-5SA-S94A-YAP plasmids were generated via restriction-based cloning from pQCXIH-Myc-YAP-5SA (Addgene plasmid #33093 36 and pCMV-Flag-YAP-5SA/S94A (Addgene plasmid #33103 42, respectively, and pBABE-PURO (Addgene plasmid #1764 59). The CCDEL deletion was introduced in the latter via overlapping PCR with the primers YAP-DEL-CC-HA-F and YAP-DEL-CC-HA-R as described in 31 ...
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bioRxiv - Biophysics 2021Quote: ... The pDrVSP-IRES2-EGFP plasmid was a gift from Yasushi Okamura (Addgene plasmid # 80333). Voltage-clamp recording of Kv7.2 current upon depolarization-induced Dr-VSP activation was performed as described 89 with an external solution containing 144 mM NaCl ...
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bioRxiv - Cell Biology 2020Quote: ... the pET30-2-GAPDH plasmids was a gift from David Sabatini (Addgene plasmid # 83910) (Pacold et al. ...
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bioRxiv - Molecular Biology 2021Quote: The CD63-pEGFP C2 plasmid was a gift from Paul Luzio (Addgene plasmid #62964)(28) ...
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bioRxiv - Molecular Biology 2021Quote: ... Cas9 plasmid (pSpCas9(BB)-2A-BFP (a modified version of PX458 (Addgene plasmid # 48138), in which eGFP is replaced for tagBFP) ...
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bioRxiv - Genetics 2020Quote: ... the NLS:LEXA (1-214aa) fragment from the pBPnlsLexA::GADflUw plasmid [35] (Addgene plasmid #26232) was cloned in frame in a myc-BAP170 (2-1688 aa ...
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bioRxiv - Molecular Biology 2020Quote: ... individual lentiviral transfer plasmids were mixed with a packaging plasmid (pMDLg/pRRE, Addgene #12251), envelope protein plasmid (pMD2.G ...
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bioRxiv - Genomics 2019Quote: ... and the pK19pA-MN plasmid was a gift from Ulrich Laemmli (Addgene plasmid # 86973). The 3XFlag-pA-Tn5-Fl plasmid was transformed into C3013 cells (NEB ...
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bioRxiv - Neuroscience 2019Quote: The plasmids used for transfection were listed (1) cis plasmid pAAV.hSyn.eGFP.WPRE.bGH (Addgene Cat# 105539) and pAAV-hSyn-eGFP (Addgene Cat# 50465) ...
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bioRxiv - Cancer Biology 2020Quote: ... Stable integrants were then further infected by modified lentiGuide-puro plasmid (Addgene plasmid # 52963) whose original puro selection marker is replaced with ZSGreen1 which is amplified from pLVX-EF1a-IRES-zsGreen1 (Clontech ...
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bioRxiv - Genomics 2020Quote: ... The PX461 and PX462 plasmids were gifts from Feng Zhang (Addgene plasmid #48140 & #48141) (35) ...
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bioRxiv - Neuroscience 2021Quote: ... Cas9 messenger RNAs (mRNAs) were transcribed in vitro from plasmid MLM3613 (Addgene, plasmid #42251). Donors were various hPNPO cDNAs assembled in the pBluescript SK(- ...
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bioRxiv - Bioengineering 2020Quote: Plasmid pcDNA3.1-GFP(1-10) was a gift from Bo Huang5 (Addgene plasmid 70219). The fragment encoding for GFP1-10 was isolated via PCR ...
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bioRxiv - Biochemistry 2020Quote: ... The plasmid encoding for S-mApple was generated from mApple-N1 (Addgene plasmid # 54567) a kind gift from Michael Davidson (Shaner et al. ...
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bioRxiv - Cancer Biology 2020Quote: A Tet-pLKO-puro plasmid was a gift from Dmitri Wiederschain (Addgene, plasmid # 21915). The shRNAs targeting mouse Pak2 were created by the cloning of annealed forward and reverse oligos synthesized based on information provided at the Broad Institute’s website (https://portals.broadinstitute.org/gpp/public/) ...
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bioRxiv - Biochemistry 2021Quote: ... pAcGHLT-B-DDB1 (plasmid #48638) and pET28-UBA1 (plasmid #32534) were obtained from Addgene. The pOPC-UBA3-GST-APPBP1 co-expression plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... the pLenti6-H2B-mCherry plasmid was a gift from Torsten Wittmann (Addgene plasmid # 89766). Lentiviral particles were produced with standard third-generation lentiviral protocol ...
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bioRxiv - Cell Biology 2021Quote: ... CMV-GFP-NMHCII-A plasmid was a gift from Robert Adelstein (Addgene plasmid # 11347) [86] ...
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bioRxiv - Immunology 2021Quote: The plasmid pHIV-EGFP was gifted by Bryan Welm & Zena Werb (Addgene plasmid #21373) and pMD2.G and psPAX2 were gifted by Didier Trono (Addgene plasmid #12259 and #12260) ...
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bioRxiv - Systems Biology 2020Quote: ... Yeast competent cells were co-transformed with a pCAS plasmid (Addgene plasmid 60847, (46)) expressing both the gRNA of interest and Streptococcus pyogenes Cas9 and a donor DNA sequence (stuffer ...
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bioRxiv - Cell Biology 2021Quote: ... The plasmids ordered were HDAC2 Flag (Addgene plasmid # 36829; http://n2t.net/addgene:36829; RRID:Addgene_36829), HDAC3 Flag (Addgene plasmid # 13819 ...
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bioRxiv - Biophysics 2021Quote: ... Plasmid containing RelA or p50 was co-transformed with pEVOL-pAzF (Plasmid #31186, Addgene), the plasmid encoding the aaRS/tRNA pair for incorporating the unnatural amino acid p-azidophenylalanine (pAzF ...
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bioRxiv - Biophysics 2020Quote: ... The pLenti6-H2B-mCherry plasmid was a gift from Torsten Wittmann (Addgene plasmid #89766) [37] and the pLenti.PGK.Lifeact-GFP.W plasmid was a gift from Rusty Lansford (Addgene plasmid #51010) ...
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bioRxiv - Biophysics 2020Quote: ... The Fubi-ChR2-GFP plasmid was a gift from Edward Boyden (Addgene plasmid #22051) [7] ...
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bioRxiv - Cell Biology 2021Quote: ... using second generation packaging plasmids pMD2.G and psPAX2 (Addgene plasmid #12259 and #12260) using Lipofectamine LTX Reagent (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... The plasmids were packaged into Lentiviral particles using the 2rd generation packaging plasmid (Addgene). 10 ml of virus containing media were concentrated with Lenti-X concentrator (Takara Bio ...
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bioRxiv - Molecular Biology 2022Quote: ... guide sequences were cloned into the pX330 sgRNA expression plasmid 90 (Addgene plasmid 42230), and the full target site was cloned into pM427 ...
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bioRxiv - Cancer Biology 2022Quote: The pA-Tn5 plasmid was a generous gift from Steven Henikoff (Addgene Plasmid #124601). The plasmid was maintained in T7 Express lysY/Ig Competent E.coli cells (NEB) ...
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bioRxiv - Neuroscience 2022Quote: ... cells were co-transfected with 750 ng of pCMV-PE2 Plasmid (Addgene Plasmid #132775) and 250 ng of assembled pU6-pegRNA-GG-acceptor using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... and the envelope plasmid pMD2.G (Addgene plasmid # 12259; http://n2t.net/addgene:12259; RRID:Addgene_12259), both gifts from Didier Trono.
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bioRxiv - Developmental Biology 2021Quote: ... Xenopus transgenic plasmids were constructed using the pI- SceI-d2EGFP plasmid backbone (Addgene 32674). First ...
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bioRxiv - Neuroscience 2021Quote: ... HA-tagged Nlgn1 plasmid was a gift from Peter Scheiffele (Addgene plasmid # 15260; RRID:Addgene_15260) (Chih et al. ...
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bioRxiv - Neuroscience 2021Quote: ... HA-tagged Nlgn1 plasmid was a gift from Peter Scheiffele (Addgene plasmid # 15260; RRID:Addgene_15260) (Chih et al. ...
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bioRxiv - Cell Biology 2022Quote: Microtubules were visualised by expressing a plasmid containing β-tubulin-mCherry (Addgene plasmid #175829).
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bioRxiv - Molecular Biology 2022Quote: ... The pAV-U6+27-Tornado-Broccoli plasmid (gift from Samie Jaffrey; Addgene plasmid 124360) was PCR amplified with Phusion polymerase using primers TBins_fwd and TBins_rev (35 cycles ...
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bioRxiv - Biophysics 2020Quote: ... both plasmids hEGFR-EGFP and mEGFP-N1 (gift from Michael Davidson, Addgene plasmid #54767) were digested with AgeI and NotI (New England Biolabs GmbH ...
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bioRxiv - Biophysics 2019Quote: The plasmids mEmerald-Zyxin-6 (Addgene plasmid # 54319; http://n2t.net/addgene:54319; RRID: Addgene_54319) and mCherry-Paxillin-22 (Addgene plasmid # 55114 ...
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bioRxiv - Synthetic Biology 2019Quote: Plasmids encoding transcription templates were constructed using pUC19-T7-3WJdB-T (Addgene plasmid #87308) as a backbone ...
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bioRxiv - Cancer Biology 2019Quote: ... and the individual CRISPR components (Cas9 expressing plasmid or sgRNA expressing plasmid: MCB306, Addgene Plasmid #89360 or MCB320 ...
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bioRxiv - Molecular Biology 2019Quote: ... HA-SUMO-2 WT plasmid was a gift from Guy Salvesen (Addgene plasmid # 48967). Flag-PIAS4 WT plasmid was a gift from Ke Shuai (Addgene plasmid # 15208) ...
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bioRxiv - Molecular Biology 2019Quote: ... HA-SUMO-1 WT plasmid was a gift from Guy Salvesen (Addgene plasmid # 48966). HA-SUMO-2 WT plasmid was a gift from Guy Salvesen (Addgene plasmid # 48967) ...
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bioRxiv - Cancer Biology 2021Quote: ... pRK5-TGFβRI-FLAG plasmid (#14833) and pCMV5B- TGFβRII plasmid (#11766) were purchased from Addgene.
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bioRxiv - Biochemistry 2021Quote: ... plasmid only or additionally with 125 ng of Lyn11-FRB-mCherry plasmid (Addgene, #38004) using Lipofectamine 3000 (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... Plasmid pAc-sgRNA-Cas9 was a gift from Ji-Long Liu (Addgene plasmid # 49330). The codons of LwaCas13a gene were optimized to Drosophila preference ...
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bioRxiv - Microbiology 2021Quote: ... RNA motif plasmid cloning backbone [(pRMB) (Addgene plasmid # 107253; http://n2t.net/addgene:107253; RRID:Addgene_107253)] ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 µg of the resulting plasmid and 0.5 µg of plasmid hCas9 (Addgene 41815) were co-transfected using 3 µl of Turbofect transfection reagent (Thermo Fisher) ...
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bioRxiv - Genomics 2021Quote: ... and cloned into the pSpCas9(BB)-2A-Puro (PX459) V2.0 plasmid (Addgene plasmid #62988) as described in Ran et al ...