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Citations for Addgene :
951 - 1000 of 10000+ citations for Rat FAM24A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... non-targeting (5’-CCTAAGGTTAAGTCGCCCTCG-3’) and DDRGK1-targeting (5’-GGCTCTGCTAGTCGGCTTTAT-3’) shRNAs were cloned into pLKO.1 puro construct (Addgene #8453) according to protocol described in Addgene (https://www.addgene.org/tools/protocols/plko/?gclid=Cj0KCQiAm5viBRD4ARIsADGUT25ZCGNPeQSFvLqSwvg2tHDkCc9zOZsLdaUffZzNTRYzI_YOlKFVQdUaAqbfEALw_wcB)
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bioRxiv - Cancer Biology 2022Quote: ... The double strand DNA coding HMGN5 shRNA and STAT3 shRNA were synthesized by Sangon Biotech (Shanghai, China) and cloned into the lentiviral vector pLKO.1-Puro (Addgene, 8453).
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bioRxiv - Microbiology 2021Quote: ... hairpin loop sequence and shRNA sequence were synthesised (IDT technologies) and annealed oligos cloned into pLKO.1 TRC cloning vector (Addgene #10878) using the unique Age1/EcoR1 sites ...
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bioRxiv - Genetics 2019Quote: ... were ordered from Integrated DNA Technologies and after phosphorylation and annealing were cloned into pLKO.1 - TRC shRNA cloning vector (Addgene #10878) into AgeI/EcoRI site ...
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bioRxiv - Cancer Biology 2020Quote: ... HEK293T cells were transfected with each TGF-β1 shRNA construct along with packaging vectors pMD2.G (0.5 μg) and psPAX2 (1.5 μg) (Addgene, Inc., Watertown, MA) using Jet Prime Reagent (Polyplus-transfection® ...
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bioRxiv - Genomics 2019Quote: ... shRNAs against KAP1 (shKAP1-B and shKAP1-D) were cloned into the pLKO.1.puro vector obtained from Addgene (http://www.addgene.org) using AgeI and EcoRI sites ...
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bioRxiv - Molecular Biology 2021Quote: ... CUG-targeting and non-targeting short hairpin RNAs (shRNAs) matching the corresponding Cas13d spacer sequences were cloned into the pLKO.1 vector (Addgene, #10878) by AgeI and EcoRI digestion and ligation of 5’-phosphorylated DNA duplexes using T4 DNA ligase (NEB ...
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bioRxiv - Cancer Biology 2020Quote: ... shRNA-mediated knockdown of CCL5 and CXCL10 in the dMMR MC38 cells was achieved using the pLKO.1 system (Addgene #10878) and containing the shRNA sequences in Supplementary Table 1.24 Stably knocked down cells were selected with 250 ug/ml hygromycin and knockdown was confirmed by Western blot.
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bioRxiv - Cancer Biology 2022Quote: ... the shRNA sequences (5′-CTCATTTAGCAGACATCGCAA-3′ (shRNA-1) and 5′-CTTTACTAGGAGACGCCAATA-3′ (shRNA-2) (Zhang et al, 2012b)) were inserted into EZ-Tet-pLKO-Puro vector (Addgene, 85966), respectively ...
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bioRxiv - Genetics 2022Quote: ... Non-target PLKO.1 shRNA viral particles were also made with the same system using the TRC non-target control lentiviral vector (Addgene # 10879). HEL and TF-1 cells were infected with EGR1 shRNA and non-target shRNA viral particles using spinfection and infected cells were selected with puromycin (2 μg/mL for TF-1 and 4 μg/mL for HEL) ...
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bioRxiv - Molecular Biology 2023Quote: ... Short hairpin RNAs (shRNAs) against CUX1 was also generated by the production and infection of lentiviral particles using pTRIPZ-DoxOn-shCUX1-5150 (Addgene, #90469) and pTRIPZ shCUX1-5328 (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: Temporally controlled knockdown of Plexin-B2 was achieved with TET-ON lentiviral vectors expressing doxycycline (Dox)-inducible shRNA targeting PLXNB2 (pLKO-Tet-On-PLXNB2–shRNA2; deposited as Addgene #98400). Puromycin selection at 1 µg/ml was used to establish transduced cell lines.
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Rab35 Governs Apicobasal Polarity Through Regulation of Actin Dynamics During Sprouting AngiogenesisbioRxiv - Developmental Biology 2022Quote: ... the destination plasmids were transfected with the three required viral protein plasmids: pMDLg/pRRE (gift from Didier Trono; Addgene plasmid # 12251), pVSVG (gift from Bob Weinberg ...
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bioRxiv - Biophysics 2020Quote: ... 1 µg of HRD plasmid and 1 µg of AAVS1 T2 CRISPR plasmid (a gift from Masato Kanemaki, Addgene plasmid #72833) were transfected into U2OS cells using FuGENE HD (Promega ...
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bioRxiv - Cell Biology 2019Quote: gRNA plasmid: Fascin gRNA target sequences were cloned into pCFD4-U6:1_U6:3tandemgRNAs plasmid (a gift from Simon Bullock (Addgene plasmid # 49411) by In-Fusion cloning (48) ...
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bioRxiv - Genetics 2019Quote: ... The UAS-uCas9 plasmid series and the full sequence of each plasmid will become available from Addgene (Addgene plasmids 127382-127387).
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bioRxiv - Immunology 2023Quote: ... The medium of the HEK 293T cells was exchanged with 1 mL fresh complete DMEM and cells were transiently co-transfected with four plasmids: 0.25 µg of a plasmid encoding the retroviral structure Gag-Pol proteins (pMDLg/pRRE, Addgene plasmid #12251), 0.5 µg of a plasmid encoding the vesicular stomatitis virus G protein (pMD2.G ...
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bioRxiv - Cancer Biology 2023Quote: ... two sgRNA sequences for KRAS as well as an sgRNA sequence to linearize the repair template plasmid were inserted into the px458_Conc3 plasmid (Addgene Plasmid #134450) using Golden Gate Cloning ...
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bioRxiv - Developmental Biology 2023Quote: ... the destination plasmids were transfected with the three required viral protein plasmids: pMDLg/pRRE (gift from Didier Trono; Addgene plasmid # 12251), pVSVG (gift from Bob Weinberg ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection was performed using 1100 ng of total DNA (500 ng transfer plasmid, 500 ng pCMVR8.74 Addgene plasmid # 22036, 100 ng pCMV-VSV-G Addgene plasmid # 8454) The day before transfection ...
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bioRxiv - Microbiology 2023Quote: ... two 6-well plates of 293T cells were transfected with plasmid pools described above, lentivirus helper plasmids (BEI: NR-52517, NR-52519, NR-52518) and VSV-G expression plasmid (Addgene #204156). This produced a VSV-G pseudotyped lentivirus pool carrying mutagenised spike sequences in their genomes ...
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bioRxiv - Neuroscience 2022Quote: ... rats received injections of AAV5.CMV.HI.eGFP-Cre.WPRE.SV40 (Addgene #105545, 0.3μl). In the Pf (females ...
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bioRxiv - Biophysics 2021Quote: The plasmid containing yeast (Saccharomyces cerevisiae) Ubr1 was purchased from Addgene (plasmid # 24506) 41 ...
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bioRxiv - Developmental Biology 2021Quote: ... EGR-G01 ES cells were transfected with two pX459 plasmids (Addgene plasmid #62988) with the target sequences (Table S1) ...
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bioRxiv - Developmental Biology 2020Quote: ... Tagmentation was performed using Tn5 synthesized from a psfTn5 plasmid (Addgene plasmid #79107) assembled with standard Illumina Tn5 adapters ...
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bioRxiv - Molecular Biology 2020Quote: ... was mixed with 4 μg of DNA RV helper plasmid (Addgene, plasmid #12371), in 1800 μl of Opti-MEM reduced serum medium (ThermoFisher ...
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bioRxiv - Developmental Biology 2022Quote: ... The LentiGuide-Puro plasmid was a gift from Feng Zhang (Addgene plasmid #52963). Sequences of each sgRNA protospacer are shown in Table S1 ...
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bioRxiv - Cancer Biology 2019Quote: Cells were assessed for Cas9 activity using the pXPR_011 plasmid (Addgene plasmid # 59702)[63] according to protocol by Doench et al ...
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bioRxiv - Microbiology 2019Quote: ... we used plasmid pSAG1::CAS9-U6::sgUPRT as a backbone (Addgene plasmid #54467) (Shen et al. ...
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bioRxiv - Cancer Biology 2019Quote: ... and envelope plasmid pMD2.G (a gift from Didier Trono, Addgene plasmid # 12259) into 293T cells using Lipofectamine 2000 (Invitrogen) ...
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bioRxiv - Genomics 2019Quote: ... The pTXB1-Tn5 plasmid was a gift from Rickard Sandberg (Addgene plasmid # 60240) and the pK19pA-MN plasmid was a gift from Ulrich Laemmli (Addgene plasmid # 86973) ...
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bioRxiv - Developmental Biology 2019Quote: ... Let-7b-GFP plasmid was a gift from Sean Morrison (Addgene plasmid # 25404) (Nishino et al. ...
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bioRxiv - Bioengineering 2019Quote: ... and were cloned into the OCT4-2A-eGFP donor plasmid (Addgene plasmid #31938). Two sgRNAs targeting at or near T stop codon (1 ...
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bioRxiv - Cancer Biology 2019Quote: ... pCMV(CAT)T7-SB100 plasmid coding for Sleeping Beauty Tranposase (Addgene plasmid # 34879) was a gift from Z ...
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bioRxiv - Genomics 2020Quote: ... The pKLV-BFP plasmid was a gift from Kosuke Yusa (Addgene plasmid #50946) (36) ...
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bioRxiv - Cell Biology 2020Quote: ... and plasmid coinjection markers pGH8 (rab-3P∷mCherry∷unc-54utr; plasmid 19359; Addgene), pCFJ104 (myo-3P∷mCherry∷unc-54utr ...
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bioRxiv - Bioengineering 2020Quote: An HSA expression plasmid was obtained from Addgene (ALB-bio-His, Plasmid #52176). E400R and K383D point mutations were introduced to the HSA sequence by the Q5 site-directed mutagenesis kit ...
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bioRxiv - Neuroscience 2021Quote: ... Plasmid encoding HA-PKCα was a gift from Bernard Weinstein (Addgene plasmid #21232). Lentiviral plasmids encoding psPAX2 (Addgene #12260 ...
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bioRxiv - Neuroscience 2021Quote: ... For each plate were used 30μg of pAdDeltaF6 helper plasmid (Addgene plasmid # 112867), 15μg of pAAV2-8 Rep-Cap plasmid (Addgene plasmid # 112864) ...
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bioRxiv - Cancer Biology 2020Quote: ... pyogenes Cas9 expression plasmid pX330-U6-Chimeric_BB-CBh-hSpCas9 (PX459) (Addgene plasmid 62988) was digested with BbsI and the gRNAs targeting E6 and E7 genes from HPV18 and HPV16 were designed using NEBuilder® HiFi DNA Assembly cloning kit (NEW ENGLAND BioLabs) ...
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bioRxiv - Cell Biology 2021Quote: The following plasmids were kind gifts: pCMV 3XFLAG-LC3A WT (Addgene plasmid #123089), pCMV 3XFLAG-LC3B WT (Addgene plasmid #123092) ...
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bioRxiv - Microbiology 2020Quote: ... and a plasmid pCMV delta R8.2 encoding HIV-1 GagPol (Addgene, plasmid #12263) using Fugene 6 (Promega ...
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bioRxiv - Developmental Biology 2021Quote: ... pLOVE-GFP plasmid was a gift from Miguel Ramalho-Santos (Addgene plasmid # 15949)14 ...
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bioRxiv - Immunology 2021Quote: Lentiviral packaging plasmids pMDLg/pRRE (Addgene plasmid #12251; http://n2t.net/addgene:12251; RRID:Addgene_12251)24 ...
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bioRxiv - Microbiology 2020Quote: Plasmids encoding Cas9 and guide RNAs were based on pX458 (Addgene plasmid 48138) (Supplementary Table 1) ...
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bioRxiv - Synthetic Biology 2021Quote: ... Plasmid pLJM1-EGFP was a generous gift from David Sabatini (Addgene plasmid #19319).
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The pan-cancer lncRNA PLANE regulates an alternative splicing program to promote cancer pathogenesisbioRxiv - Cancer Biology 2020Quote: ... pMD2.g plasmid (#12259) and pRSV-Rev plasmid (#12253) were purchased from Addgene. The pGL3-PLANE-promoter and the pGL3-PLANE-promoter-ΔE2F1-BR were purchased from Sangon Biotech (Shanghai ...
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bioRxiv - Cancer Biology 2020Quote: ... pcDNA3-HA-HIF1α plasmid was a gift from William Kaelin (Addgene plasmid #18949) 66 ...
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bioRxiv - Developmental Biology 2022Quote: ... mScarlet-I and Dendra were amplified from the plasmids pMS050 (Addgene plasmid #91826), a gift from Bob Goldstein ...
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bioRxiv - Biophysics 2022Quote: The plasmid encoding NPY-td-mOrange2 was purchased from Addgene (plasmid no. 83497). For the SN25 knockdown in PC12 cells ...