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Citations for Addgene :
951 - 1000 of 3696 citations for pLenti CMV TO Puro DEST 670 1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... The pLenti-myc-GLUT4-mCherry expressing lentivirus plasmid was a gift from Weiping Han (Addgene plasmid # 64049 ...
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bioRxiv - Cancer Biology 2020Quote: HT29 cells were infected with the pLenti-U6-tdTomato-P2A-BlasR (LRT2B) virus (Addgene, 1108545) that enables dual production of firefly luciferase and dTomato ...
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bioRxiv - Genetics 2022Quote: ... The plenti-CAG-gate-FLAG-IRES-GFP vector was a gift from William Kaelin (Addgene plasmid #107398 ...
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bioRxiv - Microbiology 2023Quote: ... and seamlessly cloned into the BamHI/XbaI sites of vector pLenti-spCas9-Blast (Addgene #52962). The lentiviruses were packaged by transfecting HEK293T cells with plasmids psPAX2 (#12260 ...
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bioRxiv - Microbiology 2023Quote: ... GFP or human c-MET cDNA were PCR amplified from plasmid pLenti-MetGFP (Addgene #37560) and seamlessly cloned into the BamHI/XbaI sites of vector pLenti-spCas9-Blast (Addgene #52962) ...
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bioRxiv - Immunology 2023Quote: ... whereas mCherry1-10-RNase L was inserted into pLenti-PGK-PuromycinR plasmid (Addgene: Plasmid #19070). The mRuby-OAS3 CDS and mRuby2 were amplified via PCR and primers (Supplemental table 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... The pLenti-AMPK Biosensor was a kind gift from Sylvia Le Dévédec (Addgene plasmid #50526). Lentiviral particles were produced by transforming pLenti constructs ...
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bioRxiv - Microbiology 2024Quote: ... To produce lentivirus carrying ACE2 expression cassette 14.2ug of pLENTI-ACE2 (ADDGENE, Watertown, MA, USA), 16ug of pCMVdR 8.2 (ADDGENE ...
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bioRxiv - Cancer Biology 2022Quote: ... CHAC1 and SLC25A39 knockout cells were generated using the pLenti-CRISPR-V2 plasmid (Addgene, 52961) encoding validated sgRNA sequences towards CHAC1 or SLC25A3974 ...
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bioRxiv - Biochemistry 2022Quote: ... Lentiviral vectors were produced by the Leuven Viral Vector Core using pLenti HsATP13A2 WT (Addgene plasmid #171485 ...
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bioRxiv - Genetics 2023Quote: ... generated by modifying pLenti-DsRed-IRES-EGFP (Addgene plasmid 92194, a gift from Huda Zoghbi), or control plasmids expressing either GFP or dsRed ...
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bioRxiv - Immunology 2024Quote: ... the sgRNA corresponding to each gene was cloned into the pLenti-sgRNA vector (Addgene #71409) with BsmBI digestion (New Englands Biolabs ...
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bioRxiv - Cancer Biology 2021Quote: ... Tet-pLKO-puro was a gift from Dmitri Wiederschain (Addgene plasmid #21915 ...
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bioRxiv - Cancer Biology 2021Quote: ... Tet-pLKO-puro (gift from D. Wiederschain, Addgene plasmid # 21915) was digested with AgeI and EcoRI and isolated by gel purification (QIAEX II Gel Extraction Kit ...
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bioRxiv - Cell Biology 2021Quote: ... for constitutive knockdown or Tet-pLKO-puro (Addgene Plasmid #21915) for periodic knockdown which was pre-linearized with AgeI-HF (NEB # R3552L ...
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bioRxiv - Cancer Biology 2021Quote: ... and ligated into a modified pBabe-Puro retroviral backbone (Addgene plasmid #1764 modified to insert FseI and AscI between BamHI and EcoRI within the MCS ...
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bioRxiv - Cancer Biology 2020Quote: ... The gBlock® was cloned into pBABE-puro (Addgene#1764).40 Retrovirus was produced using Lipofectamine™ 2000 (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... and 500 ng plentiguide puro expression vector (Addgene cat # 52963), in a final volume of 20 mL with Opti-MEM ...
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bioRxiv - Cell Biology 2022Quote: ... PCR amplified and cloned into lentiGuide-Puro vector (Addgene #52963). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: The PX459V2.0 plasmid (pSpCas9(BB)-2A-Puro) (Addgene, Watertown, MA) was used to ligate the appropriate single guide RNA sequences 5’ of the trans-activating CRISPR RNA (tracrRNA ...
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bioRxiv - Cancer Biology 2021Quote: The Tet-pLKO-puro all-in-one vector (RRID: Addgene_21915) including a puromycin resistance cassette and a tet-responsive element for expression of shRNAs was established according to a publicly available protocol (46 ...
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bioRxiv - Biochemistry 2021Quote: ... cloned into plasmid pSpCas9(BB)-2A-Puro (Addgene. ID 48139). Plasmids were transfected into B16-F1 cells ...
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bioRxiv - Systems Biology 2020Quote: ... annealed and cloned into pSpCas9(BB)-2A-Puro (Addgene #48139) vector plasmids ...
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bioRxiv - Cancer Biology 2021Quote: Tet-pLKO-puro was a gift from Dmitri Wiederschain (Addgene plasmid # 21915 ...
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bioRxiv - Cell Biology 2021Quote: ... using AAVS1 SA-2A-puro-pA donor plasmid (#22075, Addgene) as a template ...
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bioRxiv - Cancer Biology 2020Quote: The pSpCas9(BB)-2A-Puro(PX459) v2.0 plasmid (Addgene #62988) was used to clone guide sequences targeting Got2 per supplier’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: The pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid: 62988) was used ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3 µg MSCV CreERT2 puro (Addgene, 2276 ref (18)) and polybrene (8 µg/mL ...
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bioRxiv - Cell Biology 2022Quote: ... we used the 2-vector system (lenti-guide Puro; Addgene #1000000049 ...
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bioRxiv - Cancer Biology 2022Quote: ... and cloned into the EZ-Tet-pLKO-Puro vector (AddGene) using the InFusion ligation kit (Takara Bio) ...
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bioRxiv - Cancer Biology 2020Quote: ... hairpins were cloned into Tet-pLKO-puro plasmid (21915; Addgene). Lentiviral supernatants were generated by transfecting the plasmids into 293T cells and used to infect 8988T cells that were then selected for at least 7 days with 2 μg ml−1 puromycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2021Quote: ... replacing the Puromycin selection cassette in LentiGuide-Puro (Addgene #52963) with the sequence encoding mCherry ...
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bioRxiv - Cell Biology 2021Quote: ... mESCs stably transfected with pEF1α-FB-dCas9-puro(Addgene #100547) and pEF1α-BirA-V5-neo (Addgene #100548 ...
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bioRxiv - Cell Biology 2020Quote: ... using pSpCas9(BB)-2A-Puro(PX459) V2.0 vector (62988; Addgene). The following DNA sense and antisense strands of the targeting sites were annealed with each other in KOD FX neo buffer (Toyobo) ...
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bioRxiv - Genetics 2020Quote: The pSpCas9(BB)-2A-Puro (PX459) vector (Addgene plasmid #62988) (36 ...
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bioRxiv - Cancer Biology 2021Quote: ... per µg plasmid DNA with the LentiCRISPRv2-puro (Addgene #52961) or LentiCRISPRv2-blasti (55 ...
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bioRxiv - Microbiology 2021Quote: ... which was constructed based on pHAGE-puro (Addgene, plasmid 118692). The primer sequences for plasmid construction are listed in Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2021Quote: pSpCas9n(BB)-2A-Puro (PX462) vector was purchased from Addgene. For the establishment of PNKP-/- cells using CRISPR/Cas9 system ...
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bioRxiv - Genomics 2020Quote: ... before cloning into the LentiGuide-Puro plasmid (Plasmid 52963, Addgene). Positive colonies were screened by PCR and validated by Sanger sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... The vector OCT4-2A-eGFP-PKG-Puro (plasmid #31938, Addgene) was used as template to generate the donor vector ...
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bioRxiv - Cancer Biology 2020Quote: ... CD44S pBabe-puro was a gift from Bob Weinberg (Addgene plasmid # 19127 ...
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bioRxiv - Genomics 2023Quote: ... 111596)48 or pU6-sgRNA EF1Alpha-puro-T2A-BFP (Addgene, 60955).55
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bioRxiv - Cell Biology 2023Quote: ... They were inserted into the lentiCRISPRv2-puro vector (Addgene 52961) using the BsmBI restriction site ...
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bioRxiv - Cancer Biology 2023Quote: ... and TGFBR2 (5’-ccttgtagacctcggcgaag-3’) cloned into LentiCRISPRv2 puro (Addgene) (20) ...
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bioRxiv - Molecular Biology 2023Quote: ... H-RASV12 from pBABE-Puro H-RASV12 (n°12545 Addgene) was cloned in a home-made inducible vector derived from pLVX-Tight-Puro (Clontech ...
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bioRxiv - Cancer Biology 2023Quote: ... and HA-HIF2α P405A/P531A/N847Q-pBabe-puro (Addgene, 19006). To generate lentivirus for HIF mutants we subcloned HIF1α mutant from HA-HIF1α P402A/P564A-pcDNA3 (Addgene ...
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bioRxiv - Neuroscience 2022Quote: ... pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro (Addgene #71236), psPAX2 (Addgene #12260) ...
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bioRxiv - Genomics 2022Quote: ... The sgRNAs were cloned into lentiguide-Puro (Addgene ID: 52963) plasmid ...
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bioRxiv - Cancer Biology 2022Quote: ... was cloned into pLV-EF1α-IRES-puro vector (Addgene, 85132) and pLV-EF1a-IRES-Puro vector was used as control ...
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bioRxiv - Physiology 2022Quote: pBabe puro HA-PIK3CA was purchased from Addgene (plasmid #12522). This construct has an artifactual amino acid change in its coding sequence (I143V ...