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Citations for Addgene :
851 - 900 of 3696 citations for pLenti CMV TO Puro DEST 670 1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The gene was cloned into pLenti-DsRed-IRES-EGFP vector (Addgene plasmid number 92194)64 by restriction digestion using Afe1 and BamH1 (New England BioLabs (NEB)) ...
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bioRxiv - Microbiology 2023Quote: ... combined with either Cas9 expression vector plenti-Cas9-blast (Feng Zhang, Addgene plasmid # 52962) (79 ...
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bioRxiv - Cancer Biology 2024Quote: HT29 cells previously infected with pLenti-U6-tdTomatato-P2A-BlasR (LRT2B) virus (Addgene, 1108545) that enables dual production of firefly luciferase and TdTomato were used ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293T cells were transfected with the lentiviral vector pLenti-PGK-ER-KRASG12V (Addgene, 35635) and the packaging plasmids pCMV-VSV-G (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... and pLenti-PGK-Neo-PIP-FUCCI (Fluorescent Ubiquitination-based Cell Cycle Indicator) (Addgene # 118616) was used for accurate cell cycle phase indicator [34] ...
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bioRxiv - Neuroscience 2023Quote: ... macaque: NM_001257303.1) into the lentiviral backbone pLenti-EF-FH-TAZ-ires-blast (Addgene #52083) with EcoRI/SalI sites.
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bioRxiv - Immunology 2024Quote: ... 1.5μg each) and a dCas9-VPR plasmid (pLenti-dCas9-VPR-blast, Addgene, #96917; 1μg) as described above ...
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bioRxiv - Cell Biology 2022Quote: ... TagRFP-Nir2 and TagRFP-Nir3 were subcloned into pLenti-CMVie-IRES-BlastR (#119863, Addgene) and constructs were co-transfected with pMD2G/psPAX2 into HEK-293T cells to produce viral particles as described previously (Carreras-Sureda et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... pLenti-mCherry-CDK4KTR plasmid was a gift from Hee Won Yang (Addgene, plasmid #126680). Lentiviral particles harbor the H2b-mcherry construct were used to generate cells with stable nuclear mCherry ...
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bioRxiv - Cell Biology 2024Quote: ... Expi293F cells were grown and transfected with the pLenti-palmGRET reporter plasmid (Addgene #158221) (10 ...
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bioRxiv - Molecular Biology 2024Quote: ... was amplified from pSMART-Rps3-HaloTag7-3xFlag (gift from Heeseon An) and P2A-puro was amplified from lentiCRISPRv2 puro (Addgene, cat # 98290). The amplified PCR products were cloned using GeneArt™ Seamless Cloning and Assembly Enzyme Mix (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... and YTHDC1 were generated by cloning the shRNA (RNAi Consortium shRNA Library) from pLKO.1-puro into the pLKO.1-blast backbone (Addgene #26655).
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: P-Lenti CMV/TO SV40 small + Large T (w612-1) was a gift from Eric Campeau (Addgene plasmid # 22298). For packaging the virus ...
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bioRxiv - Microbiology 2020Quote: The lentiviral vector was prepared by recovering the culture supernatant of 293T cells transfected with CSII-CMV-FNF-DsRed together with expression plasmids for HIV-1 Gag-Pol and Rev (pCMVR8.74, Addgene plasmid #22036 ...
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bioRxiv - Neuroscience 2024Quote: ... AAV injections consisted of a 1:10 cocktail of Cre [AAV5-CMV-HI-eGFP-Cre.WPRE.SV40 (Addgene plasmid no. 105545) packaged at UPenn Vector Core 2.5 × 1014 GC ml−1] and KORD [AAV8-HSyn-DIO-HA-KORD-IRES-mCitrine (Addgene plasmid no ...
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bioRxiv - Developmental Biology 2020Quote: ... and its control pBabe-Puro-IKBalpha-WT (Addgene 15290) were used to generate transgenic lines analyzed in Fig ...
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bioRxiv - Cell Biology 2020Quote: ... pMK243 (Tet-OsTIR1-PURO) was purchased from Addgene (#72835) and the OsTIR1 fragment was removed by BglII and MluI digestion ...
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bioRxiv - Neuroscience 2021Quote: We used pSpCas9 (BB)-2A-Puro (PX459) V2.0 (Addgene) and modified it in order to replace the CMV promoter by the EF1 alpha promoter ...
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bioRxiv - Cancer Biology 2021Quote: ... sgRNA was then cloned into the lentiGuide-puro (Addgene plasmid # 52963 ...
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bioRxiv - Cancer Biology 2020Quote: ... Tet-pLKO-puro (Wiederschain et al., Addgene plasmid #21915), CALCRL MISSION shRNA (shCALCRL#1 ...
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bioRxiv - Cancer Biology 2020Quote: ... All guide RNAs were cloned into LentiGuide-Puro (Addgene) according to the Zhang laboratory protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Not1-linearized pBabe-puro largeTcDNA plasmid (Addgene plasmid # 14088) was transfected into mouse and naked mole-rat skin fibroblasts and selected with puromycin for 2-4 weeks (mouse cells took ~2 weeks ...
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bioRxiv - Cell Biology 2021Quote: ... LentiCRISPRv2 puro was a gift from Brett Stringer (Addgene plasmid # 98290 ...
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bioRxiv - Cell Biology 2021Quote: ... and independently cloned into CROPseq-puro-v2 (Addgene #127458) as described previously (9).
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bioRxiv - Cell Biology 2021Quote: ... pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene plasmid #62988) from Feng Zhang ...
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bioRxiv - Cancer Biology 2020Quote: ... or pSpCas9(BB)-2A-Puro (PX459) vector (Addgene, 48139) from Feng Zhang ...
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bioRxiv - Cell Biology 2021Quote: ... Lentiviral backbone was based on lentiGuide-Puro (Addgene # 52963), with AU-flip as in53.
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bioRxiv - Molecular Biology 2022Quote: ... pU6-sgRNA-EF1Alpha-puro-T2A-BFP (Addgene, Plasmid #60955) was used to express sgKAT and sgNC sgRNAs ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid pSpCas9(BB)-2A-Puro (PX459) V2.0 (#62988, Addgene) with puromycin resistance was used as the target plasmid to carry the guide RNA ...
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bioRxiv - Genetics 2021Quote: ... lentiGuide-Puro was a gift from Feng Zhang (Addgene plasmid # 52963 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the U6-sgRNA segment from LentiGuide-Puro(Addgene #52963) was PCR amplified and transferred to pBabe-SV40-puro to generate a pBabe-U6sgRNA-SV40-puro vector with NheI and XhoI cloning sites.
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bioRxiv - Microbiology 2020Quote: ... and pSpCas9(BB)-2A-Puro (PX459, Addgene ID 48139). pX458 and pX459 were digested with BbsI and dephosphorylated with FastAP Thermosensitive Alkaline Phosphatase ...
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bioRxiv - Genetics 2020Quote: ... and pSpCas9n(BB)-2A-Puro (PX461) (plasmid#48141, Addgene) respectively ...
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bioRxiv - Cancer Biology 2021Quote: ... pSpCAS9 (BB)-2A-Puro (PX459) V2 plasmids (Addgene; #62988) were used following the protocol described by Ran et al.54 ...
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bioRxiv - Cell Biology 2020Quote: ... MSCV-CreERT2-Puro (Plasmid #22776) were obtained from Addgene. pLKO-Puro shHDAC4 1 (TRCN0000314667 ...
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bioRxiv - Cell Biology 2020Quote: ... pSpCas9(BB)-2A-Puro v2.0 (Plasmid #62988 from Addgene). sgRNAs were designed using CHOPCHOP v2 (46) ...
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bioRxiv - Microbiology 2020Quote: ... Primers were cloned into LentiGuide-Puro (Addgene plasmid #52963) by phosphorylating ...
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bioRxiv - Genomics 2021Quote: ... LentiGuide-mCherry was generated by modifying lentiGuide-puro (Addgene) to remove a puromycin-resistant gene and replace it with mCherry ...
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bioRxiv - Microbiology 2023Quote: ... and lentiCRISPRv2 puro (Plasmid #98290) were obtained from Addgene. pCDNA3.1-DRD2 was generated by subcloning Drd2 into the pCDNA3.1 backbone using EcoRI and XhoI ...
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bioRxiv - Bioengineering 2023Quote: ... and GG-EBNA-EEA-5guides-PGK-Puro (Addgene #102898) used for reprogramming experiments have been described previously17 ...
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bioRxiv - Cancer Biology 2024Quote: ... and pLX302 TdTomato-V5 puro (Addgene, recombined from #82404). For organoid transduction ...
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bioRxiv - Cancer Biology 2024Quote: ... pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro (Addgene plasmid # 71236 ...
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bioRxiv - Systems Biology 2023Quote: ... and cloned into pSpCas9(BB)P2A-Puro (Addgene #48139) vector plasmids ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... and cloned into BsmBI-digested pLentiRfxGuide-Puro (Addgene #138151) via Gibson Assembly ...
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bioRxiv - Immunology 2023Quote: ... and ligating primers into LentiGuide-Puro (Addgene, plasmid 52963). Colonies were validated by Sanger sequencing using pLK0.1/hU6 promoter primer (Eton Biosciences ...
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bioRxiv - Cell Biology 2022Quote: ... was cloned into a lentiGuide-puro vector (Addgene 52963). Lentiviral supernatants ...
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bioRxiv - Molecular Biology 2023Quote: ... we adapted the plentiGuide-puro vector (Addgene plasmid # 52963)51 to incorporate a second guide RNA expression cassette as described previously52 ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg of pMtnA FLAG-IntS6 puro (Addgene #195076) or pMtnA FLAG-IntS12 puro (Addgene #195077 ...
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids expressing IntS6 (pMtnA FLAG-IntS6 puro, Addgene #195076) or IntS12 (pMtnA FLAG-IntS12 puro ...
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bioRxiv - Cancer Biology 2023Quote: ... pSpCas9(BB)-2A-Puro (PX459) V2.0 (pX459_puro, Addgene #62988), pSpCas9(BB)-2A-Hygro (pX459_hygro ...