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Citations for Addgene :
901 - 950 of 2123 citations for Mouse EGF Like Repeat And Discoidin I Like Domain Containing Protein 3 EDIL3 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: Mouse Bpnt2 cDNA was cloned into the pBABE-puro (Addgene #1764) retroviral vector using BamHI and SalI restriction sites ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... a mouse KO sgRNA pooled library (Addgene: #1000000096, 10sgRNA per gene) was amplified at 1000X fold coverage ...
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bioRxiv - Physiology 2020Quote: ... the mouse N-Shh sequence was then cloned in pRRLsin.MND.MCS.WPRE (Addgene) that had been previously digested by NheI and PmlI ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse Mannosidase II amino acids 1-116 was amplified from Addgene plasmid #65261 using a forward primer that contains an XbaI site and a reverse primer with an MluI site and further subcloned upstream of the mCherry open reading frame.
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bioRxiv - Cell Biology 2022Quote: Mouse SEPT6-GFP construct was purchased from Addgene (Addgene plasmid# 38296) and was cloned into the pLVX-IRES-puro vector (Clontech) ...
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bioRxiv - Immunology 2023Quote: pcDNA3-N-Flag-NLRP3 plasmid encoding mouse NLRP3 (Addgene plasmid # 75127) was a gift from Bruce Beutler61 ...
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bioRxiv - Molecular Biology 2023Quote: The Mouse Improved Genome-wide Knockout CRISPR Library v2 (Addgene #67988) collection sgRNAs in lentiviral vectors targeting 18 ...
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bioRxiv - Cell Biology 2020Quote: ... The mammalian expression vector containing the coding sequence for EB1-2xEGFP was purchased (Addgene; Plasmid # 3782741) The pShuttle mCherry-Tubulin adenoviral vector (Tub-WT ...
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bioRxiv - Synthetic Biology 2020Quote: ... A synthetic toolkit (MoClo-YTK) containing yeast parts were gifts from the Dueber Lab (Addgene #:1000000061). Characterization plasmids consisted of 8 parts ...
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bioRxiv - Genomics 2019Quote: ... sgRNA containing pLG1 vectors along with the pHR-SFFV-KRAB-dCas9-P2A-mCherry (Addgene, plasmid #60954) were used to generate lentivirus using the Lenti-Pac HIV expression packaging kit (GeneCopoeia) ...
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bioRxiv - Microbiology 2020Quote: Oligos containing gRNA sequence were annealed and ligated into pX458 (Addgene #48138, gift from Feng Zhang). Cells were transfected with pX458 constructs using Lipofectamine 3000 (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviruses were produced in 293T cells with a second-generation packaging system containing psPAX2 (#12260; Addgene) and pMD2.G (#12259 ...
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bioRxiv - Biochemistry 2022Quote: FUS SYQG LC containing a TEV cleavable N-terminal histidine tag (RP1B FUS LC, AddGene #127192), full-length FUS with an N-terminal histidine tag and maltose-binding protein fusion (pTHMT FUS 1-526 ...
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bioRxiv - Neuroscience 2022Quote: ... Virus (0.25-1 μl) containing either AAV5: hSyn-DIO-hM3Dq-mCherry (excitatory DREADD; Addgene, Watertown MA), AAV5 ...
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bioRxiv - Microbiology 2021Quote: ... solution containing 2 μg of TF-responsive reporters driving Firefly luciferase (pGL3-3xAP1-luciferase (Addgene, 40342), pGL3-NF-κB-luciferase (Promega) ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids containing the coding sequences of human SLC46A1 (pDONR221_SLC46A1) and SLC46A3 (pDONR221_SLC46A3) were purchased from Addgene. Custom plasmids (pTwist-CMV ...
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bioRxiv - Physiology 2022Quote: The pcDNA3.1 plasmid containing the PinkFlamindo coding sequence (PinkFlamindo-pcDNA3.1; (59) was obtained from Addgene (#102356). The following primers were used for PCR amplification ...
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bioRxiv - Cell Biology 2022Quote: ... we Gibson assembled a PCR product containing CMV-StrepKDEL-IRES-SBP (amplified from Addgene plasmid #65295) to the SalI/MluI digested piggyback backbone (a generous gift from Jonathon Nixon-Abell ...
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bioRxiv - Immunology 2019Quote: Hem1 expression constructs containing C-terminal 3xFLAG-v5 tags were generated in a pcDNA3.1 backbone (Addgene) using Gateway cloning technology (Thermo Fisher ...
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bioRxiv - Cancer Biology 2019Quote: ... HCT116-Dnmt1Δ3-5 cells were transfected with pcDNA3 vector containing WT full length DNMT1 (36939, AddGene) and empty pcDNA3 vector as a transfection control (10792 ...
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bioRxiv - Cancer Biology 2020Quote: ... Blasticidin-resistant cells were subsequently transduced with viral supernatant containing LentiGuide-Puro plasmid (Addgene plasmid #52963) with ligated selected sgRNA ...
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bioRxiv - Developmental Biology 2020Quote: ... A plasmid containing estrogen receptor alpha (pEGFP-C1-ERα) was obtained from Michael Mancini (Addgene #28230) and mutated into a constitutively active form (pEGFP-C1-ERαY537S)29 using the Q5® Site-Directed Mutagenesis Kit (New England Biolabs ...
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bioRxiv - Neuroscience 2020Quote: ... and ligated into plasmid DNA containing the Cas9 enzyme and a puromycin resistance cassette (PX459, Addgene) followed by exonuclease treatment ...
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bioRxiv - Synthetic Biology 2020Quote: Plasmids containing the dCas9-effector fusions were derived from the dCas9-KRAB vector backbone (Addgene #110820) and modified by Gibson Assembly with their respective effectors from sources listed above ...
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bioRxiv - Bioengineering 2021Quote: ... and assembled in a Golden Gate assembly containing 6.25 ng pU6-atgRNA-GG-acceptor (Addgene #132777), purified PCR product (approximately 2-to-4-fold molar excess) ...
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bioRxiv - Molecular Biology 2020Quote: ... Lentiviral control vector containing a scrambled shRNA (shSCR) was ordered from Addgene (scramble shRNA, Addgene # 1864).
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bioRxiv - Cancer Biology 2021Quote: Neonatal human dermal fibroblasts (HDFns) were transduced with lentiviruses containing pCHAC-mt-mKeima (Addgene plasmid #72342) (Lazarou et al. ...
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bioRxiv - Cancer Biology 2021Quote: The oligos containing the gene-specific sgRNA target were cloned into the LentiCRISPRv2 Blasticidin (Addgene, 83480) as previously described 53 ...
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bioRxiv - Cell Biology 2022Quote: ... mCherry-cGAS was inserted into an HA-containing vector (gift from Qing Zhong; Addgene plasmid #280274) (53 ...
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bioRxiv - Physiology 2022Quote: ... a DNA block containing sgEGFP-tRNA-Hnf4a-sg2 was PCR-amplified using pGTR plasmid (Addgene #63143) as a template and primers listed in Table S2 (see also Fig ...
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bioRxiv - Developmental Biology 2022Quote: ... A plasmid containing the full-length human FOS cDNA (NM_005252.4) was purchased from Addgene (Plasmid #59140). The full-length FOS cDNA was cloned into the pCS2 vector and FOS mRNA was generated using the mMessage mMachine Sp6 kit (Ambion ...
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bioRxiv - Cell Biology 2023Quote: ... the oligos containing the gene-specific sgRNA target were cloned into the LentiCRISPRv2 Blasticidin (Addgene, #83480). The CRISPR/Cas9 primer sequences were as followed:
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bioRxiv - Cancer Biology 2023Quote: For HDTVI the following plasmids were used: pCMV(CAT)T7-SB100 containing SB100X transposase (Addgene #34879). For Myr-AKT and YAP5SA expression the pSBbi-Puro vector from Addgene (#60523 ...
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bioRxiv - Molecular Biology 2022Quote: Oligonucleotides containing gRNA sequence were Annealed and later cloned into lentiGuide-puro plasmid (Addgene Cat # 52963) followed by the published protocol 39 ...
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bioRxiv - Neuroscience 2023Quote: ... a solution containing either AAV9.hSyn.eGFP.WPRE.bGH or AAV9.hSyn.HI.eGFP-Cre.WPRE.SV40 (Addgene; #50465-AAV9 or #105540-AAV9) was pipetted onto the hippocampus within the slice (1 μL on each hemisphere ...
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bioRxiv - Cell Biology 2024Quote: ... DNA fragments containing MAP3K1 (MEKK1) or kinase-dead MAP3K1 were excised from pCDNA-MEKK1 plasmids (Addgene #12181 and #12180 ...
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bioRxiv - Bioengineering 2023Quote: A synthetic toolkit (MoClo-YTK) containing yeast parts were gifts from the Dueber Lab (Addgene #1000000061). Expression vectors for yeGFP ...
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bioRxiv - Cancer Biology 2023Quote: ... Plasmids containing the AXL phosphosite mutations were generated from an AXL-IRES-Puro vector (Addgene #65627) using site directed mutagenesis ...
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bioRxiv - Microbiology 2023Quote: The eSpCas9(1.1) gene containing two nuclear localization signals (NLS) was obtained from Addgene (Plasmid #71814) and cloned into the pST32 vector ...
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bioRxiv - Cell Biology 2024Quote: ... Lentiviral plasmids containing the relevant guides were co-transfected with helper plasmids psPAX2 (Addgene Ref. 12260) and pCMV-VSV-G (Addgene ...
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bioRxiv - Genetics 2024Quote: ... These gRNAs were cloned into a plasmid containing Cas9 and a BFP reporter (Addgene Plasmid # 64216) and cutting efficiency was tested in 293T after transient transfection using lipoD293T and the T7E1 assay ...
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bioRxiv - Genetics 2024Quote: ... These gRNAs were cloned into a plasmid containing Cas9 and a BFP reporter (Addgene Plasmid # 64216) and cutting efficiency was tested in 293T after transient transfection using lipoD293T and the T7E1 assay ...
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bioRxiv - Cell Biology 2023Quote: ... each well was transfected with 1.5 µg of the plasmid containing the YFP-Parking gene (Addgene, 23955 ...
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bioRxiv - Neuroscience 2020Quote: ... the target sequence (5’-GGGTGAAGATCCTGTTCAATA-3’) was shuttled to the pLKO.1-Hygromycin vector (Addgene, #24150). For human astrocytes ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Optimal gRNA (5’-CCTACGAACTCCGGTGTCAG-3’) was cloned into the pSPCas9(BB)-2A-GFP plasmid (Addgene #48138) as described previously by Ran et al.,21 and introduced into ciPTEC using PolyPlus JetPrime ...
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bioRxiv - Neuroscience 2022Quote: ... The loops were shuttled into pCSF107mT-GATEWAY-3’-3HA (gift from Todd Stukenberg, Addgene plasmid # 67616) using Gateway LR clonase ...
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bioRxiv - Cancer Biology 2019Quote: ... A mixture of 3 transfection plasmids were produced by combining 2 µg pMDG.2 (Addgene #12259) (Addgene ...
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bioRxiv - Immunology 2020Quote: ... Guide sequences (listed in Supplementary Table 3) were cloned into the lentiCRISPR v2 plasmid (Addgene #52961) and lentiviral particles were generated in 293T cells (ATCC ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse primer NT: 5’-CGGGATCCCTATTGAGTTCTTTTGTGCTC-3’) and cloned into the mApple-C1 vector (Addgene plasmid # 54631) using the XhoI and BamHI restriction sites ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Optimal gRNA (5’-GTCGTAAAGCTGGAGAACGG-3’) was cloned into the pSPCas9(BB)-2A-GFP plasmid (Addgene #48138) as described previously by Ran et al ...