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Citations for Addgene :
651 - 700 of 3696 citations for pLenti CMV TO Puro DEST 670 1 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... The TERT-immortalized human melanocyte cell C283T was infected with pCW-Cas9-Blast from Addgene followed by introduction of lentiGuide-Puro (Addgene) expressing pooled gRNAs ...
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bioRxiv - Cancer Biology 2021Quote: ... pSpCas9 (BB)-2A-Puro (PX459) (Addgene # 62988) by using complimentary oligonucleotide pairs ...
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bioRxiv - Biochemistry 2021Quote: ... 10 μg of plasmid (pBABE-puro, Addgene #1764 or pBabe-puro Ras V12 ...
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bioRxiv - Cell Biology 2020Quote: ... and pGLTR-X-Puro (Addgene plasmid #58246) plasmids (Pfeiffenberger ...
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bioRxiv - Bioengineering 2020Quote: ... pLVX puro TRIM21-GFP (Addgene Plasmid #1786) was obtained as a gift from Gaudenz Danuser ...
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bioRxiv - Systems Biology 2024Quote: ... pBABE 3xHA-MKK6-EE puro (Addgene, 216791) was created by PCR cloning of 3xHA-MKK6-EE (Addgene ...
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bioRxiv - Systems Biology 2024Quote: ... and cloned into lentiGuide-Puro (Addgene 52963) by the Golden Gate method as described in (https://media.addgene.org/cms/filer_public/3e/e1/3ee1ce9c-99f9-4074-9a28-109d34971471/zhang-lab-sam-cloning-protocol.pdf) ...
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bioRxiv - Systems Biology 2024Quote: pBABE FLAG-IKK2-EE puro (Addgene, 216790) was created by PCR cloning of IKK2-EE (Addgene ...
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bioRxiv - Neuroscience 2024Quote: ... TetO-NGN2-eGFP-Puro plasmid (Addgene, #79823), or one of the RCOR3-shRNA-mCherry-BSD plasmids or the TetO-SOX9-NFIB-mPlum-Puro plasmid using Polyethyleneimine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... TetO-NGN2-eGFP-Puro plasmid (Addgene, #79823) using Polyethyleneimine (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... pSpCas9(BB)–2A-puro (pX459) (Addgene, #48139).
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bioRxiv - Cancer Biology 2024Quote: ... The pLVX-TetOne-Puro-hAXL (Addgene, 124797) backbone was used as a starting point prior to undergoing a series of modifications ...
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bioRxiv - Molecular Biology 2023Quote: ... or pMtnA FLAG-IntS12 puro (Addgene #195077) plasmid was transfected using Effectene (Qiagen 301427 ...
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bioRxiv - Cancer Biology 2023Quote: ... or pHAGE-puro empty vector (Addgene #118692) (128 ...
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bioRxiv - Genomics 2023Quote: ... with pBABE-puro SV40 LT plasmid (Addgene # 13970 ...
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bioRxiv - Developmental Biology 2023Quote: ... using Puro-Cas9 donor (Addgene plasmid 58409) as template and the following primer pairs ...
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bioRxiv - Molecular Biology 2023Quote: ... pBabe-Puro-IKBalpha-wt (Addgene plasmid #15290), dCas9-p65 ...
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bioRxiv - Developmental Biology 2023Quote: ... and lenti MS2 grna-Puro (Addgene; 52963), while the second library was only cloned into the lenti MS2 grna-Puro backbone by the MSKCC Gene Editing & Screening Core Facility ...
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bioRxiv - Cancer Biology 2023Quote: ... and cloned into lentiCRISPRv2 puro vector (Addgene) using Gibson Assembly Master Mix (New England Biolabs) ...
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bioRxiv - Cancer Biology 2023Quote: Reporter plasmid (pBOB-EF1-FastFUCCI-Puro, Addgene-plasmid # 86849 ...
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bioRxiv - Microbiology 2023Quote: ... into the lentiGuide-puro plasmid (Addgene, 52963). These plasmids were confirmed by Sanger sequencing before production of lentiviral particles ...
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bioRxiv - Genomics 2023Quote: ... CROPseq-Guide-Puro backbone (Addgene ID: 86708), T7 DNA ligase (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... and cloned into the CROPseq-Puro (Addgene) backbone by Golden Gate cloning with BsmBI (NEB ...
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bioRxiv - Genomics 2023Quote: ... linearized pLV-EF1a-IRES-Puro (Addgene, #85132) plasmid by Gibson assembly ...
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bioRxiv - Cancer Biology 2024Quote: LentiGuide-Puro (Addgene, Cat. 52963, RRID: Addgene_52963) and lentiCas9-Blast (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: LentiGuide-Puro (Addgene, Cat. 52963, RRID: Addgene_52963) and lentiCas9-Blast (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: ... a modified PEA1-Puro plasmid (Addgene #171991) was designed to introduce an intronic ACE2 single nucleotide polymorphism (SNP) ...
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bioRxiv - Cancer Biology 2024Quote: HA-VHL wt-pBabe-puro (#19234, Addgene) plasmid was used for transient transfections ...
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bioRxiv - Neuroscience 2024Quote: ... encoding pTet-O-Ngn2-puro (Addgene #52047) and FUΔGW-rtTa (Addgene #19780) ...
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bioRxiv - Neuroscience 2024Quote: ... 2.) piRES-puro vector (Addgene Cat# 25728) with EcoRI and NotI sites for site-directed mutagenesis experiments.
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bioRxiv - Cell Biology 2021Quote: ... and pLenti CMV rtTA3 Hygro (w785-1) used for creating tetracycline-inducible cell lines were gifts from Eric Campeau (w762-1: Addgene plasmid #26434 ...
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bioRxiv - Genomics 2020Quote: ... Plasmids for human LINE-1 expression: pBS-L1PA1-CH-mneo (CMV-LINE-1) was a gift from Astrid Roy-Engel (Addgene plasmid # 51288 ...
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bioRxiv - Neuroscience 2024Quote: ... AAV8-Ef1a-mCherry-IRES-Cre (1×1013 vg/ml #55632) and AAV2-CMV-EGFP (1×1013 vg/ml, #105530) were purchased from Addgene.
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bioRxiv - Molecular Biology 2022Quote: ... pLenti PE2-BSD was a gift from Hyongbum Kim (Addgene plasmid # 161514) (Kim et al. ...
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bioRxiv - Systems Biology 2020Quote: ... then put into pLenti-mp2 backbone (Pantelis Tsoulfas from Addgene plasmid # 36097).
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bioRxiv - Cancer Biology 2021Quote: ... the pLenti-CMVie-IRES-BlastR (pCIB) plasmid was obtained from Addgene (#119863). pCIB-2xHA was generated by cloning a double HA tag with a flanking NotI site in pCIB ...
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bioRxiv - Microbiology 2020Quote: ... EMCV IRES (derived from pLenti-DsRed_IRES_EGFP (Addgene, #92194, gift from Huda Zoghbi)) ...
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bioRxiv - Microbiology 2024Quote: ... pLenti-spBsmBI-sgRNA-Hygro (a gift from Rene Maehr, Addgene plasmid #62205), or pLentiGuide-zeo (a gift from Rizwan Haq ...
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bioRxiv - Neuroscience 2023Quote: ... then inserted into an AgeI-/EcoRI-linearized pLenti-hSyn backbone (Addgene #86641) by Gibson assembly ...
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bioRxiv - Neuroscience 2023Quote: ... then inserted into an AgeI-/EcoRI-linearized pLenti-hSyn backbone (Addgene #86641) by Gibson assembly ...
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bioRxiv - Cell Biology 2023Quote: ... Guides were cloned into puromycin-resistant pLenti-CRISPRV2 (Addgene; catalog no. 49535) as described in (Sanjana et al. ...
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bioRxiv - Molecular Biology 2023Quote: ... we modified a lentiviral plasmid (pLenti CMVie-IRES-BlastR, Addgene accession: #11963) by replacing the blasticidin resistance cassette ...
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bioRxiv - Molecular Biology 2024Quote: ... IRF2BP2 OE lentivirus was produced using plenti backbone plasmid (Addgene plasmid # 17448) containing the full-length coding sequence cloned from hAPC ...
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bioRxiv - Microbiology 2024Quote: Lentiviruses were generated using pLenti-PACT-HA and pMD2.G (Addgene #12259), pRSV-Rev (Addgene #12253) ...
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bioRxiv - Cancer Biology 2024Quote: ... as well as the lentiviral construct of interest (pLenti-Lifeact-tdTomato, RRID:Addgene_64048 ...
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bioRxiv - Cell Biology 2024Quote: ... pLenti-X1-hygro-mCherry-RAMP4 was a gift from Jacob Corn (Addgene plasmid # 118391 ...
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bioRxiv - Biochemistry 2020Quote: ... according to manufacturer’s instruction and then transferred to pLX302 lentiviral destination vector (addgene) or pDEST-CMV-N-EGFP vector (pDEST-CMV-N-EGFP was a gift from Robin Ketteler (Addgene plasmid # 122842) using recombination utilising the LR-Clonase (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: The pAAV-GfaABC1D::Cas9-HA-Ezr was generated by first replacing the CMV promoter from pX601-AAV-CMV::NLS-saCas9-NLS-3xHA-bGHpA;Bsal-sgRNA (Addgene plasmid #61591) with the GFAP promoter (GfaABC1D) ...
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bioRxiv - Bioengineering 2020Quote: ... The miniCMV-SpCas9 (SpCas9) construct was generated by replacing the CMV promoter with a miniCMV promoter in the pX551-CMV-SpCas9 plasmid (Addgene No. 107024) via AgeI and XbaI restriction enzyme sites ...
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bioRxiv - Microbiology 2020Quote: ... This vector expresses an endoplasmic reticulum (ER)-retained truncated Env-EGFP fusion protein. For linearization experiments (Fig. 5) we used plasmid pNL-EGFP/CMV/WPREdU3 (pNL-CMV-GFP) (Addgene, MA, USA). pNL4-3/Luc/Ori and pNL4-3/Luc/Kan were produced by molecular cloning into pNL4-3/Luc ...