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6251 - 6300 of 10000+ citations since 2019

• Comprehensive mutagenesis maps the effect of all single codon mutations in the AAV2 rep gene on AAV production

  Nina K. Jain, Pierce J. Ogden, George M. Church, bioRxiv - Synthetic Biology 2023

  Quote: ... pCAG-Luc (ssAAV, Addgene plasmid #83281 ...
• Multiplexed effector screening for recognition by endogenous resistance genes using positive defense reporters in wheat protoplasts

  Salome Wilson, et al., bioRxiv - Plant Biology 2023

  Quote: ... using primers (Zenodo Supplemental Data S6) with BpiI recognition sites and 4bp overhang to facilitate assembly into universal level 0 vector pAGM9121 (Addgene Plasmid #51833). Any BsaI recognition sites were removed as per Grutzner & Marillionnet ...
• Molecular mechanisms controlling the biogenesis of the TGF-β signal Vg1

  P. C. Dave P. Dingal, et al., bioRxiv - Developmental Biology 2023

  Quote: ... Commercially available sequence for bovine enterokinase (Addgene Plasmid #49048) was a gift from Hans Brandstetter (74) ...
• OSBP-mediated PI(4)P-cholesterol exchange at endoplasmic reticulum-secretory granule contact sites controls insulin secretion

  Styliani Panagiotou, et al., bioRxiv - Cell Biology 2023

  Quote: ... Plasmids pLJM1-FLAG-GFP-OSBP was from Addgene (Plasmid #134659; (Lim et al., 2019)) ...
• Cilium proteomics reveals Numb as a positive regulator of the Hedgehog signaling pathway

  Xiaoliang Liu, et al., bioRxiv - Cell Biology 2023

  Quote: ... two sequences targeting exon 1 of Numb (5’-GAAAGACGTTTATGTCCCAG-3’ and 5’-GGAAGCTACACTTTCCAGTG-3’) were individually cloned into plasmid pSpCas9(BB)-2A-Puro (PX459) V2.0 (Addgene #62988). These guide constructs were then electroporated into mESCs using the Lonza Nucleofector 2b Device and Cell Nucleofector Kit (Lonza #VAPH-1001) ...
• Distinct states of nucleolar stress induced by anti-cancer drugs

  Tamara A. Potapova, et al., bioRxiv - Cell Biology 2023

  Quote: ... Plasmids encoding the human UBF gene tagged with EGFP and nucleolin gene tagged with EGFP were obtained from Addgene (plasmids # 26672 and #28176 ...
• Regulation of the circadian clock in C. elegans by clock gene homologs kin-20 and lin-42

  Melisa L. Lamberti, et al., bioRxiv - Neuroscience 2023

  Quote: ... and cloned into vector punc-122::dsRed (coel::RFP Plasmid #8938, Addgene) to generate punc-122::isoform-b::dsRed (6.8 kb) ...
• A Facile Method for Determining Lanthipeptide Stereochemistry

  Youran Luo, Shuyun Xu, Wilfred A. van der Donk, bioRxiv - Biochemistry 2023

  Quote: ... This method is also applicable to the expression and purification of mCylLS (plasmid available at Addgene ID #208760).
• Emergent functional behaviors of ribozymes in oxychlorine brines indicate Mars could host a unique niche for molecular evolution

  Tanner G. Hoog, et al., bioRxiv - Biochemistry 2023

  Quote: The β-lactamase plasmid (pExp-Bla, Addgene plasmid #112561) was expressed in E ...
• Structural and mechanistic insights into disease-associated endolysosomal exonucleases PLD3 and PLD4

  Meng Yuan, et al., bioRxiv - Biochemistry 2023

  Quote: ... PLD3 KO cell line was generated from HEK293BlueTM hTLR9 by transfecting 2 μg hSpCas9-sgRNA expressing plasmid (Addgene #99154) cloned with gRNA sequence 5′-guccucauucuggcgguugu-3′ ...
• Oligomerisation mediated by the D2 domain of DTX3L is critical for DTX3L-PARP9 reading function of mono-ADP-ribosylated androgen receptor

  Carlos Vela-Rodríguez, et al., bioRxiv - Biochemistry 2023

  Quote: ... with the CMV promoter in pKH3 (Addgene #12555) and additional cloning sites ...
• Oligomerisation mediated by the D2 domain of DTX3L is critical for DTX3L-PARP9 reading function of mono-ADP-ribosylated androgen receptor

  Carlos Vela-Rodríguez, et al., bioRxiv - Biochemistry 2023

  Quote: Lentiviral vector pLH3 was constructed by replacing the U6 promoter in pLKO.1 GFP shRNA (Addgene #30323) with the CMV promoter in pKH3 (Addgene #12555 ...
• Engineering Regionally-Activated Drugs for Neuroscience

  Zhimin Huang, et al., bioRxiv - Bioengineering 2023

  Quote: ... and subcloned into the vector pRSETb115 (Addgene ID 89536) with a N-terminal His-tag through Gibson assembly at the BamHI and EcoRI site ...
• Role of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins

  Katharine R. Page, et al., bioRxiv - Cell Biology 2023

  Quote: ... wash buffer containing 500 nM SUMOEu1 protease (Addgene #149333) was added to the beads and left to incubate for 30 minutes with mixing at 4°C ...
• Role of a holo-insertase complex in the biogenesis of biophysically diverse ER membrane proteins

  Katharine R. Page, et al., bioRxiv - Cell Biology 2023

  Quote: ... Then His14-Avi-SUMOEu1-tagged anti-GFP nanobody (Addgene #149336) was immobilized onto the washed magnetic beads for 30 min with mixing at 4°C using a ratio of 27 μg for every 80 μl beads ...
• Spartin-mediated lipid transfer facilitates lipid droplet turnover

  Neng Wan, et al., bioRxiv - Cell Biology 2023

  Quote: ... The gRNA was cloned into pX458 (48138; Addgene) as described previously (33) ...
• Calcium influx rapidly establishes distinct spatial recruitments of Annexins to cell wounds

  Mitsutoshi Nakamura, Susan M. Parkhurst, bioRxiv - Cell Biology 2023

  Quote: ... ML1Nwt-eGFP was amplified from ML1Nwt_in_pEGFP-C1 (Addgene #67797; (Li et al., 2013)) and cloned into pSqh5’+3’UTR as a 5′ StuI-3′ XbaI fragment.
• Calcium influx rapidly establishes distinct spatial recruitments of Annexins to cell wounds

  Mitsutoshi Nakamura, Susan M. Parkhurst, bioRxiv - Cell Biology 2023

  Quote: ... Lact-C2 was amplified from Lact-C2-GFP (Addgene #22852) and fused 5’ to StFP ...
• Molecular determinants of SEC61A1 mutant R236C in two patient-derived cellular platforms

  Matthias Weiand, et al., bioRxiv - Cell Biology 2023

  Quote: ... pCXLE-hSK (Addgene #27078), and pCXLE-hUL (Addgene #27080 ...
• The ULK1 effector BAG2 regulates autophagy initiation by modulating AMBRA1 localization

  Devanarayanan Siva Sankar, et al., bioRxiv - Cell Biology 2023

  Quote: ... cloned in pX330-U6-Chimeric_BB-CBh-hSpCas9 (gift from Feng Zhang (Addgene plasmid # 42230; http://n2t.net/addgene:42230; RRID:Addgene_42230)) ...
• Overriding Mendelian inheritance in Arabidopsis with a CRISPR toxin-antidote gene drive that impairs pollen germination

  Yang Liu, et al., bioRxiv - Evolutionary Biology 2023

  Quote: ... the sgRNA cassette (pU6-SmR-gRNA scaffold-U6 terminator) was amplified from the plasmid pHEE401E (Addgene plasmid #71287) and inserted into the double-digested XF675 binary vector (digested with EcoRI and HindIII ...
• Duplication and neofunctionalization of a horizontally-transferred xyloglucanase as a facet of the red queen co-evolutionary dynamic

  Victoria Attah, et al., bioRxiv - Evolutionary Biology 2023

  Quote: ... Synthesised DNA fragments were cloned into vector PGWB514 (Addgene 74856) (84 ...
• Neuronal exosomes transport a miRNA/RISC cargo to preserve germline stem cell integrity during energy stress

  Christopher Wong, Elena M. Jurczak, Richard Roy, bioRxiv - Developmental Biology 2023

  Quote: ... The plasmids were cloned into the pCFJ151 MosSCI vector targeting the ttTi5605 targeting region (Addgene) (41) ...
• Characterization of Target Gene Regulation by the Two Epstein-Barr Virus Oncogene LMP1 Domains Essential for B-cell Transformation

  Bidisha Mitra, et al., bioRxiv - Cancer Biology 2023

  Quote: ... were cloned into lentiGuide-Puro (Addgene, catalog #52963) or pLenti SpBsmBI sgRNA Hygro (Addgene ...
• Non-redundant roles for the human mRNA decapping cofactor paralogs DCP1a and DCP1b

  Ivana Vukovic, et al., bioRxiv - Biochemistry 2023

  Quote: pMD2.G (Addgene, Watertown, MA, Addgene plasmid # 12259)
• Addressing the dNTP bottleneck restricting prime editing activity

  Karthikeyan Ponnienselvan, et al., bioRxiv - Biochemistry 2023

  Quote: ... Then PCR products were Gibson-cloned into the PEmax mRNA plasmid (Addgene plasmid #204472) digested with RsrII and XhoI enzymes ...
• Addressing the dNTP bottleneck restricting prime editing activity

  Karthikeyan Ponnienselvan, et al., bioRxiv - Biochemistry 2023

  Quote: ... This PCR product was digested with BamHI and BsrGI and cloned into pET-21a-PEmax-6His (Addgene plasmid #204471) digested with the same enzymes ...
• Addressing the dNTP bottleneck restricting prime editing activity

  Karthikeyan Ponnienselvan, et al., bioRxiv - Biochemistry 2023

  Quote: ... and inserted into PEmax mRNA plasmid (Addgene plasmid #204472) digested by SalI and EcoRI ...
• The N-terminal region of DNMT3A combines multiple chromatin reading motifs to guide recruitment

  Hannah Wapenaar, et al., bioRxiv - Biochemistry 2023

  Quote: ... purchased from Addgene originated by the Landry lab ...
• The N-terminal region of DNMT3A combines multiple chromatin reading motifs to guide recruitment

  Hannah Wapenaar, et al., bioRxiv - Biochemistry 2023

  Quote: ... Human DNMT3A1 and DNMT3L constructs were PCR amplified from cDNA expression constructs (Addgene #35521, #35523) and cloned by ligation dependant cloning into x6His-MBP-TEV or 6xHis-MBP-GFP expression vectors ...
• ER-export and ARFRP1/AP-1-dependent delivery of SARS-CoV-2 Envelope to lysosomes controls late stages of viral replication

  G.J Pearson, et al., bioRxiv - Cell Biology 2023

  Quote: ... with HA-TurboID amplified by PCR from 3xHA-TurboID-NLS_pCDNA3 (Addgene #107171) and inserted with a Gly-Gly-Gly-Ser linker either side of the HA-TurboID sequence ...
• Top-down control of flight by a non-canonical cortico-amygdala pathway

  Chandrashekhar D. Borkar, et al., bioRxiv - Neuroscience 2023

  Quote: ... we used AAV5-CAG-Flex-GcaMP6f-WPRE-SV40 (Addgene 100835 ...
• Top-down control of flight by a non-canonical cortico-amygdala pathway

  Chandrashekhar D. Borkar, et al., bioRxiv - Neuroscience 2023

  Quote: ... we used AAV5-EF1a-DIO-eNpHR3.0-EYFP (Addgene 26966, a gift from Karl Deisseroth). Control mice for optogenetic experiments were injected with AAV5-EF1a-DIO-EYFP (Addgene 27056 ...
• Top-down control of flight by a non-canonical cortico-amygdala pathway

  Chandrashekhar D. Borkar, et al., bioRxiv - Neuroscience 2023

  Quote: ... we injected AAV5-EF1a-DIO-hChR2(H134R)-EYFP (Addgene 20298, a gift from Karl Deisseroth) in the DP of Vglut2-IRES-cre mice and retrograde beads in PAG subcolumns to label CeM projectors ...
• Top-down control of flight by a non-canonical cortico-amygdala pathway

  Chandrashekhar D. Borkar, et al., bioRxiv - Neuroscience 2023

  Quote: ... AAV5-hSyn-DIO-mCherry (Addgene 50459, a gift from Bryan Roth) was injected in the DP.
• Top-down control of flight by a non-canonical cortico-amygdala pathway

  Chandrashekhar D. Borkar, et al., bioRxiv - Neuroscience 2023

  Quote: ... AAV5-hSyn-DIO-mCherry (Addgene 50459, a gift from Bryan Roth) was injected in the DP.
• Top-down control of flight by a non-canonical cortico-amygdala pathway

  Chandrashekhar D. Borkar, et al., bioRxiv - Neuroscience 2023

  Quote: ... CRH-IRES-Cre and SOM-IRES-Cre mouse lines were injected with AAV5-EF1a-DIO-EYFP (Addgene 27056, a gift from Karl Deisseroth). In the same mice ...
• Top-down control of flight by a non-canonical cortico-amygdala pathway

  Chandrashekhar D. Borkar, et al., bioRxiv - Neuroscience 2023

  Quote: ... we used AAV-hSyn-DIO-hM4D(Gi)-mCherry (Addgene 44362, a gift from Bryan Roth). For Cre-dependent GCaMP6f expression ...
• Top-down control of flight by a non-canonical cortico-amygdala pathway

  Chandrashekhar D. Borkar, et al., bioRxiv - Neuroscience 2023

  Quote: ... we used AAV5-hSyn-DIO-hM3D(Gq)-mCherry (Addgene 44361, a gift from Bryan Roth). For Cre-dependent expression of inhibitory DREADDs ...
• Diagnostic and Therapeutic Microbial Circuit with Application to Intestinal Inflammation

  Liana N. Merk, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... HrpR was amplified from pBW115 (Addgene 61434), pHrpL was amplified from pBW412hrpL-cIgfp (Addgene 61438) ...
• Vagus nerve stimulation recruits the central cholinergic system to enhance perceptual learning

  Kathleen A. Martin, et al., bioRxiv - Neuroscience 2023

  Quote: ... 0.50 µL of AAV5.FLEX.ArchT.tdTomato (diluted 1:2 with dPBS; Addgene number: 28305) was injected in basal forebrain of ChAT-Cre animals using the coordinates described above ...
• Vagus nerve stimulation recruits the central cholinergic system to enhance perceptual learning

  Kathleen A. Martin, et al., bioRxiv - Neuroscience 2023

  Quote: ... doublefloxed.hChR2(H134R).EYFP.WPRE-HGHpA (diluted 1:2 with dPBS; Addgene number: 20298) was injected into auditory cortex as described above in ChAT-Cre animals ...
• Hyaluronic acid turnover controls the severity of Cerebral Cavernous Malformations in bioengineered human micro-vessels

  Teodor E. Yordanov, et al., bioRxiv - Cell Biology 2023

  Quote: ... which were co-transfected with the gRNA containing lentiCRISPRv2 vector together with the packaging vectors pMDLg/pRRE (Addgene; 12251), pRSV-Rev (Addgene ...
• Concerted regulation of skeletal muscle metabolism and contractile properties by the orphan nuclear receptor Nr2f6

  Dimitrius Santiago P.S.F. Guimarães, et al., bioRxiv - Biochemistry 2023

  Quote: ... or PGC-1α 2kb promoter (Handschin et al. 2003) (Addgene #8887), normalization plasmid coding for Renilla luciferase (pRL-SV40 ...
• Distinct subpopulations of ventral pallidal cholinergic projection neurons encode valence of olfactory stimuli

  Ronald Kim, et al., bioRxiv - Neuroscience 2023

  Quote: ... AAV9.RAM.d2tTA.TRE.Flex.tdTomato (FLEX-RAM; Addgene # 84468 ...
• Leucine zipper-based sorting system enables generation of multi-functional CAR T cells

  Scott E. James, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... DNA constructs were generated via standard molecular biology techniques including overlap extension PCR and restriction site-based cloning and ligated directly into pENTR1a no ccdB (Addgene 17398) or the MMLV-based retroviral vector LZRS-Rfa (Addgene 31601) ...
• Turn-On Protein Switches for Controlling Actin Binding in Cells

  Unyime M. Effiong, et al., bioRxiv - Synthetic Biology 2023

  Quote: ... Plasmids encoding NS3a and CP5 were obtained from Addgene (Addgene plasmid #133608 and #133614). Cloning was performed similarly to the peptide-based CAST designs through PCR amplification and Gibson Assembly of the fragments.
• TBK1 is ubiquitinated by TRIM5α to assemble mitophagy machinery

  Bhaskar Saha, et al., bioRxiv - Cell Biology 2023

  Quote: ... the Renilla luciferase plasmid (pRL-SV40, Addgene plasmid #27163) was a gift from Ron Prywes and ISRE luciferase plasmid was gifted by Dr ...
• KIF1C, an RNA transporting kinesin-3, undergoes liquid-liquid phase separation through its C-terminal disordered domain

  Qi Geng, et al., bioRxiv - Cell Biology 2023

  Quote: A plasmid for expression of full-length human KIF1C (pKIF1C-GFP) was a gift from Anne Straube (Addgene plasmid #130977107). All truncated versions of KIF1C (see Resource Table) ...
• Biodesign of Frugal CRISPR Kits for Equitable and Accessible Education

  Marvin C. Collins, et al., bioRxiv - Bioengineering 2023

  Quote: ... Plasmid encoding the aeBlue gene was ordered from Addgene (#117846). The aeBlue chromoprotein gene was amplified in a PCR reaction using a KAPA Hi-Fi kit ...