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Citations for Addgene :
6351 - 6400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: SgRNA sequences for target genes were cloned into pXPR502 (Addgene 96923) and screened by sanger sequencing ...
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bioRxiv - Immunology 2023Quote: ... was co-transfected with packaging plasmids pCAG-VSVG and psPAX2 (Addgene plasmids 35616 and 12260, respectively). Briefly ...
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bioRxiv - Immunology 2023Quote: The human genome-wide CRISPRa-V2 library (Addgene 1000000091) was co-transfected with packaging plasmids pCAG-VSVG and psPAX2 (Addgene plasmids 35616 and 12260 ...
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bioRxiv - Immunology 2023Quote: SgRNA sequences for target genes were cloned into pLentiguide puro (Addgene 52963) and confirmed by sanger sequencing ...
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bioRxiv - Immunology 2023Quote: ... The shRNAs were cloned separately into LentiCRISPRv2-GFP (modified from Addgene) and transiently co-transfected with psPAX2 (12260 ...
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bioRxiv - Immunology 2023Quote: ... Addgene)and pMD2.G(12259, Addgene)in MOLT4 cells for 24 hours ...
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bioRxiv - Microbiology 2023Quote: Sequences of all 16 plasmids used for the generation of wild type SA11 strain were obtained from Addgene (https://www.addgene.org/Takeshi_Kobayashi/)5 ...
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bioRxiv - Immunology 2023Quote: ... Plasmid pFGM1 contains a gentamycin resistance gene from Addgene and cloned into the pMF230 plasmid creating a constitutively active eGFP plasmid with 10 mg/ml gentamycin resistance ...
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bioRxiv - Immunology 2023Quote: ... Fresh virus of (MSCV-myc-CAR-2A-Thy1.1, Anjana Rao, Addgene: 127890) containing chimeric antigen receptor (CAR ...
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bioRxiv - Immunology 2023Quote: ... coli containing the pMF230 plasmid containing a constitutively active promoter and eGFP were ordered from Addgene and cultivated on 100 uM ampicillin LB agar plates ...
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bioRxiv - Immunology 2023Quote: ... The amplicons were cloned into human IGHG1 or IGKC or IGLC expression vectors (Addgene number 80795 ...
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bioRxiv - Genomics 2023Quote: ... The amplified fragments were then inserted into SbfI/AgeI site of the pLS-SceI vector (Addgene, 137725) using NEBuilder HiFi DNA Assembly mix (NEB) ...
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bioRxiv - Genomics 2023Quote: ... 2.5 μg/flask of pMD2.G (Addgene 12259), and 5 μg/flask of psPAX2 (Addgene 12260 ...
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bioRxiv - Genetics 2023Quote: ... was inserted into the pMK1200 vector (pMK1200 was a gift from Martin Kampmann & Jonathan Weissman (Addgene plasmid # 84219 ...
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bioRxiv - Genetics 2023Quote: ... DNA plasmids used for CLYBL locus targeting were pC13N-iCAG.copGFP (Addgene 66578), pZT-C13-L1 (Addgene 62196) ...
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bioRxiv - Genetics 2023Quote: ... the FKBP coding sequence was amplified from the PM-FRB-Cerulean-T2A-FKBP-5-ptase plasmid (Addgene 40897 ...
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bioRxiv - Genetics 2023Quote: ... the saCas9 and FRB coding sequences were amplified from the pX601 plasmid (Addgene 61591, a kind gift from Dr. Feng Zhang) (Ran et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... The gRNA oligonucleotides were inserted into the U6>sgRNA(F+E) vector which was a gift from Lionel Christiaen (Addgene plasmid # 59986 ; http://n2t.net/addgene:59986 ; RRID:Addgene_59986) 52 ...
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bioRxiv - Developmental Biology 2023Quote: ... TdTomato-LifeAct tdTomato-Lifeact-7 was a gift from Michael Davidson (Addgene plasmid # 54528 ...
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bioRxiv - Developmental Biology 2023Quote: ... The Tex10 guide RNA sequence (refer to Table S16) was inserted into the PX330-puro plasmid (Addgene plasmid #62988) to obtain the PX330-Tex10 plasmid.
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bioRxiv - Developmental Biology 2023Quote: ... Then the loxP-dsRed-loxP fragment was cloned from pMSCV-loxP-dsRed-loxP-3xHA-Puro-WPRE (Addgene plasmid #32703). The loxP-dsRed-loxP fragment and the above modified pJet1.2 plasmid were digested with the restriction enzymes AgeI (NEB ...
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bioRxiv - Developmental Biology 2023Quote: Donor Plasmids for insertion of 5xtetO-pEF1-H2B-CITRINE reporter into the genome were constructed by subcloning 5xtetO-pEF1-H2B-CITRINE-PolyA from PhiC31-Neo-ins-5xtetO-pEF-H2B-Citrine-ins (Addgene# 78099)14 with right and left homology arms (500 bps each ...
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bioRxiv - Developmental Biology 2023Quote: ... Plasmid with mCherry-2A-rTetR-CBX7 was created by subcloning mCherry-2A-rTetR (Addgene # 78101)14 and CBX7 into lentiviral expression vector backbone pLVU-tTR-KRAB (Addgene# 11645)71 ...
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bioRxiv - Developmental Biology 2023Quote: ... Plasmid with mCherry-2A-rTetR-CBX7 was created by subcloning mCherry-2A-rTetR (Addgene # 78101)14 and CBX7 into lentiviral expression vector backbone pLVU-tTR-KRAB (Addgene# 11645)71 ...
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bioRxiv - Developmental Biology 2023Quote: ... we used psPAX2 (Addgene# 12260) and pMD2.G (Addgene# 12259 ...
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bioRxiv - Cell Biology 2023Quote: ... lentivirus was produced by co-transfecting 293T cells with psPAX2 (Addgene; #12260), pMD2.G (Addgene ...
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bioRxiv - Cell Biology 2023Quote: ... pMD2.G (Addgene; #12259) and pLV retroviral vectors containing different PXN cDNAs ...
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bioRxiv - Cell Biology 2023Quote: MDCK cells expressing GFP-Rab19 on the Rab19 KO background were lysed and incubated with GST-tagged anti-GFP nanobody (recombinantly produced from pGEX6P1-GFP-Nanobody, Addgene Plasmid #61838) or with free GST for negative control ...
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bioRxiv - Cell Biology 2023Quote: The siRNA-resistant CPAP cDNA containing plasmid was obtained from Addgene (#46390). Full-length and C-terminal coding sequences (residues 895-1338 CP3 ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-Rab5A(Q79L) (35140, Addgene); mCherry-Rab5A(Q79L ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-Rab8A (24898, Addgene), R5BD-YFP 87 ...
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bioRxiv - Cell Biology 2023Quote: ... GFP-Rab10A (49472, Addgene); GFP-Rab8A (24898 ...
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bioRxiv - Cell Biology 2023Quote: ... mCherry-Rab5A(Q79L) (35138, Addgene); CFP-Rab5A(S34N ...
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bioRxiv - Developmental Biology 2023Quote: ... The synthetic Cas9 mRNA was transcribed from the XbaI linearized pT3TS-nls-zCas9-nls vector (Addgene #46757)(74 ...
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bioRxiv - Developmental Biology 2023Quote: ... The oligos (5-taggCCTGACAGTACTCACGAC -3’ and 5’-aaacGTCGTGAGTACTGTCAGG -3’) were annealed and ligated into the pT7-gRNA vector (Addgene #46759)(74 ...
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bioRxiv - Immunology 2023Quote: ... pLKO.1 puro vector (Addgene, #8453) expressing control or Il5ra shRNAs ...
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bioRxiv - Immunology 2023Quote: ... pUC18R6KT-mini-Tn7T-Km (#64969) and pUC18R6K-mini-Tn7T (#64958) plasmids were purchased from Addgene. The Tn7 vector (pSAC1 ...
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bioRxiv - Neuroscience 2023Quote: ... 60-100 nl AAV2-CAG-FLEX-GCaMP6f viruses (Vigene, custom prepared) or AAV1-CAG-FLEX-GCaMP6f viruses (Addgene, 100835-AAV1, 3x dilution) were delivered unilaterally into each of the following targeted brain regions as described previously (Fang et al. ...
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bioRxiv - Neuroscience 2023Quote: ... and NGN2 (pTet-O-Ngn2-puro; Addgene, #52047). We amplified and harvested these plasmids by using EndoFree Plasmid Maxi Kit (Qiagen ...
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bioRxiv - Neuroscience 2023Quote: ... and AAV5-Ef1a-double-floxed-hChR2(H134R)-mCherry-WPRE-hGHpA (Addgene 20297, titer 1.4e+13) was used for optogenetic activation ...
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bioRxiv - Neuroscience 2023Quote: ... AAV1_hSyn1-SIO-stGtACR2-FusionRed (Addgene 105677, 2.1e+12 after 10 fold dilution) was used for optogenetic inactivation ...
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bioRxiv - Neuroscience 2023Quote: ... were obtained from Addgene.
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bioRxiv - Molecular Biology 2023Quote: ... or pMtnA FLAG-IntS12 puro (Addgene #195077) plasmid was transfected using Effectene (Qiagen 301427 ...
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bioRxiv - Molecular Biology 2023Quote: ... Hml promoter (Hy_pHml eGFP SV40; Addgene #132645), CG8620 promoter (Hy_pCG8620 eGFP SV40 ...
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bioRxiv - Molecular Biology 2023Quote: ... or Ubi-p63e promoter (Hy_pUbi-p63e eGFP SV40; Addgene #132650) were described previously.27,54 Reporter plasmids expressing eGFP under the control of the RnrS promoter (Hy_pRnrS eGFP SV40 ...
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bioRxiv - Molecular Biology 2023Quote: ... were described previously.27,54 Reporter plasmids expressing eGFP under the control of the RnrS promoter (Hy_pRnrS eGFP SV40, Addgene #195062) or Ana promoter (Hy_pAna eGFP SV40 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Ad4E4orf6 (Addgene #64221), 53BP1-DN1S (Addgene #131045) ...
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bioRxiv - Synthetic Biology 2023Quote: ... and VSV-G envelope (pMD2.G, gift from Didier Trono (Addgene plasmid # 12259 ...
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bioRxiv - Synthetic Biology 2023Quote: The CIDAR MoClo Parts Kit was a gift from Douglas Densmore (Addgene kit # 1000000059). CIDAR MoClo Extension ...
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bioRxiv - Synthetic Biology 2023Quote: ... the BBR1 origin of replication was amplified from pSEVA331Bb (Addgene #78269). A silent mutation was introduced in the Rep protein to remove a BbsI restriction site (G186A) ...