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Citations for Addgene :
551 - 600 of 2724 citations for Mouse Macrophage expressed gene 1 protein MPEG1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... reporter under the constitutive elongation factor 1-α (EF1α) promoter and upstream of a P2A linker followed by the tdTomato fluorescent protein (gift from Kazuhiro Oka;Addgene plasmid # 72486 ...
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bioRxiv - Bioengineering 2021Quote: ... We acquired psPAX2 encoding lentiviral packaging proteins and PMD2.G encoding a lentiviral envelop protein from Addgene (plasmid no ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant mouse E1 (Addgene plasmid # 32534), E2 (pGEX4T-1-UbcH5b) ...
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bioRxiv - Molecular Biology 2020Quote: ... Mouse Ddx5 WT(Addgene plasmid #88869) and Lys144 to Asn mutant(Addgene plasmid #88870 ...
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bioRxiv - Neuroscience 2022Quote: ... and mouse IgG1 Fc (Addgene #28216) and IgG2a Fc (Addgene #114492) ...
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bioRxiv - Neuroscience 2023Quote: ... pCDNA3-mouse PKA-RIalpha-mEGFP (Addgene plasmid # 45525 ...
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bioRxiv - Neuroscience 2023Quote: ... pcDNA3-mouse PKA-RIbeta-mEGFP (Addgene plasmid # 45526 ...
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bioRxiv - Molecular Biology 2021Quote: ... the zebrafish codon optimized gene of TagRFP658 was cloned into pTol2-UAS-zArchon1-KGC-GFP-ER2 plasmid (Addgene #108427) described before33 by swapping zArchon1-KGC-GFP-ER2 using SpeI and AscI sites.
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bioRxiv - Genomics 2020Quote: sgRNA was cloned into pGL3-sgRNA expression vector carrying a U6 promoter and an EGFP reporter gene (Addgene, #107721). CBE-targeted genomic sites are indicated in Supplementary Table 1 ...
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bioRxiv - Immunology 2021Quote: ... two individual guides per gene were cloned into the LentiSAM vector (a gift from Feng Zhang, Addgene plasmid #75112) and MelJuSo MPH cells were stably transduced with these guides or with the empty LentiSAM as a control ...
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bioRxiv - Cell Biology 2020Quote: The gRNAscaffHygroR vector was constructed by PCR amplification of the Hygromycin resistance gene from pBABE-hygro-hTERT (Addgene # 1773) plasmid and cloning into the EGFP_SV40PA vector downstream of the OmEF1a promoter followed by the modified guide RNA scaffold sequence PCR amplified from gRNA_GFP-T2 (Addgene # 41820).
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bioRxiv - Cancer Biology 2020Quote: Knockout clones were generated via CRISPR/Cas9 gene targeting by transient transfection with a plasmid encoding hSpCas9 (Addgene #42230) and a plasmid encoding specific gRNA (see below for detail) ...
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bioRxiv - Biochemistry 2021Quote: ... genes encoding GDH2 and PEPCK were amplified from GS115 genomic DNA and cloned into pGHYB vector (Addgene plasmid #87447) (Yang et al. ...
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bioRxiv - Microbiology 2020Quote: ... The invariant gRNA scaffold was removed together with the puromycin resistance gene and replaced with ACE2 (Addgene Plasmid #1786) followed by a P2A-coupled blasticidin resistance gene driven by a cytomegalovirus promotor ...
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bioRxiv - Molecular Biology 2020Quote: ... all the sgRNAs targeting genes of interest as well as controls were cloned into LRG2.1 (derived from U6-sgRNA-GFP, Addgene: 108098 - as described in ref (Tarumoto et al. ...
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bioRxiv - Microbiology 2021Quote: ... was assembled in-frame with a hygromycin resistance gene into pHR-PGK (a gift from Wendell Lim, Addgene #7912093), generating pHR-PGK-dCas9-SunTag-P2A-HygR.
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bioRxiv - Molecular Biology 2020Quote: The gene encoding ALD-A along with 1.0 kb of its promoter was cloned in-into pIB3 vector (#25452, Addgene) and transformed into GS115 and Δmxr1 ...
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bioRxiv - Cell Biology 2020Quote: ... RNP complex (Cas9 with tracrRNA for LMNB1 gene) (IDT) and 400 ng of LMNB1_mTagRFP-T HDR plasmid (Addgene 114403). Nucleofection was done with the pulses DN-100 ...
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bioRxiv - Genomics 2022Quote: ... Two gRNAs (one targeting row and one targeting the white gene) was cloned into pCFD4d plasmid (Addgene plasmid #83954) (as described in the protocol “cloning two gRNAs into plasmid pCFD4” ...
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bioRxiv - Cell Biology 2022Quote: SIMC1 knockout clones were generated via CRISPR/Cas9 gene targeting by transient transfection of a hSpCas9 encoding plasmid (Addgene) and a pcDNA-H1 plasmid encoding specific sgRNA ...
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bioRxiv - Microbiology 2021Quote: ... The wildtype human HIF1α gene was obtained from pcDNA3-HIF1α plasmid (Addgene 18949, a gift from William Kaelin (45)) ...
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bioRxiv - Microbiology 2020Quote: The production of high titer Human sgRNA Brunello lentiviral library which contains 4 sgRNA per gene (15) (Addgene #73178), was performed by transfecting HEK293T cells in five 15 cm tissue culture plates using PEI (Polyethylenimin Linear ...
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bioRxiv - Microbiology 2021Quote: ... and the nourseothricin resistance gene was amplified from plasmid pMOD3-mNeptune2-nat (78) (Addgene plasmid #120335; http://n2t.net/addgene:120335; RRID:Addgene 120335) using primers “F Nat” and “R Nat” with inclusion of 5’ tails encoding overlap between vector and nourseothricin amplicons ...
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bioRxiv - Cell Biology 2022Quote: ... guide RNAs targeting a gene of interest (Table S1) were cloned into pKLV-U6gRNA(BbsI)-PGKpuro2ABFP (Addgene plasmid #50946), using the BbsI restriction sites ...
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bioRxiv - Cell Biology 2019Quote: ... The h-Fyn gene source was from pRK5-c-Fyn (a gift from Dr. Filippo Giancotti, Addgene plasmid # 16032). Biosensors were constructed using gene fusion ...
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bioRxiv - Biochemistry 2021Quote: ... we transfected HEK293T cells with Cas9 and sgRNA targeting the gene encoding for the CCT5 subunit (Addgene eSpCas9(1.1) vector ...
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bioRxiv - Neuroscience 2020Quote: ... control of the strong ubiquitous CAG promoter was generated by replacing the tdTomato gene in the Allen Brain Institute’s Ai9 targeting vector (CAG-floxed tdTomato; Addgene plasmid 22799 ...
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bioRxiv - Neuroscience 2021Quote: ... Adeno-associated virus containing the GCaMP7f gene (pGP-AAV9-syn-FLEX-jGCaMP7f-WPRE, 104488-AAV9, Addgene, Watertown, MA, USA) was loaded into a glass micropipette with a tip diameter of 40–50 µm attached to a Nanoject II injection system (Drummond Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... To achieve Tmem120a knockout in primary SVF cells loxP sites recombination was induced in vitro by the delivery of the Cre recombinase gene fused with GFP in AAV virus (RRID:Addgene_49056). As a control AAV with the GFP-only construct was used (RRID:Addgene_49055) ...
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bioRxiv - Microbiology 2020Quote: ... The SNAP gene was amplified from pVpr.IN.SNAP and the mScarlet (WT) (Bindels et al., 2016) gene from the mScarlet C1 vector (Addgene #85042) and placed N-terminal to OR3 into PCR linearized pWPI EF1-alpha OR3 IRES puro backbone by Gibson assembly ...
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bioRxiv - Microbiology 2020Quote: ... the human ACE2 gene (Miaolingbio #P5271) was PCR-amplified and cloned into the pLV-EF1a-IRES-blast (Addgene #85133). The human TMPRSS2 and DPP4 gene (Sino Biological #HG13070-CM ...
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bioRxiv - Immunology 2021Quote: ... a pair of sgRNAs were designed for a target sequence in exon 3 of the mouse CD274 (coding for PD-L1) gene using the online tool at http://crispr.mit.edu and were cloned into pSpCas9n(BB)-2A-GFP (Addgene, #48140). LECs were transfected with the vectors using polyethylenimine as described (Hsu and Uludag ...
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bioRxiv - Molecular Biology 2021Quote: ... The gene fragment for Cas12k was cloned into the bacterial expression plasmid pET-His6-StrepII-TEV LIC (Addgene: #29718). Cas12k ...
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bioRxiv - Molecular Biology 2023Quote: CRISPR mediated mutagenesis of target genes was performed by transducing K562 cells stably expressing Cas9 (lentiCas9-Blast, Addgene #52962) with lentivirus expressing sgRNAs (lentiGuide-Puro ...
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bioRxiv - Molecular Biology 2022Quote: ... The gene encoding Cas3/I-G was cloned into pET Strep II TEV LIC cloning vector (1R, Addgene #29664). All the clones were confirmed by Sanger sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... Gene knockdown was performed in a sub-clonal K562 cell line stably expressing dCas9-KRAB-BFP (Addgene plasmid #102244), generously provided by Eric J ...
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bioRxiv - Cancer Biology 2022Quote: ... Next day the transfection master mix which contained 10µg of CRISPR construct containing sgRNA targeting the gene of interest (or empty lentiCRISPRv1-puro, #49535, Addgene), 7.5µg of pSPAX2 (#12260 ...
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bioRxiv - Microbiology 2023Quote: ... while long-term single-gene knockout cell lines were generated using sgRNAs in pLenti SpBsmBI sgRNA Hygro (Addgene, 62205) containing a hygromycin resistance cassette ...
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bioRxiv - Systems Biology 2023Quote: ... we disrupted the endogenous LEU2 gene in Yα1867 yeast using the M3926 leu2::KanMX3 disruptor converter plasmid with G418 resistance (Addgene). M3926 was digested with BamHI (New England Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... a gene-specific crRNA was cloned into the Lenti-CRISPR-V2 backbone (gift from Feng Zhang; Addgene plasmid #52961). This plasmid ...
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bioRxiv - Cancer Biology 2023Quote: The Brunello genome-wide gRNA library contains 76,441 gRNAs targeting 19,114 genes and was obtained from Addgene (Cat# 73178). Lentivirus containing the Brunello library was generated and used to transduce NB4 cells ...
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bioRxiv - Bioengineering 2023Quote: ... which were then introduced along with an IRES sequence into an existing plasmid encoding the gvpA gene (Addgene #197588) through Gibson assembly ...
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bioRxiv - Neuroscience 2023Quote: ... The Cas9 vector used for double-strand break generation in the Prnp gene is available from Addgene (plasmid #78621) [40] ...
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bioRxiv - Cell Biology 2023Quote: ... sgRNAs for SAM-mediated endogenous gene overexpression were cloned into the lenti_sgRNA(MS2)_zeo_backbone (kind gift from Feng Zhang, Addgene #61427) as reported previously (Joung et al. ...
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bioRxiv - Cancer Biology 2023Quote: ... The CD19-CAR-28z-2A-EGFP gene cassette from pSLCAR-CD19-28z (a gift from Scott McComb: Addgene 135991)53 was cloned into the MTOR donor vector by Gibson assembly without the 3xFLAG ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The Cas9 vector used for double-strand break generation in the Prnp gene is available from Addgene (plasmid #78621) (27) ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... PCR was used to amplify the U6-crRNA scaffold and the DiCas7-11 gene sequence from pDF0114 (Addgene #172508) and pDF0159 (Addgene #172507) ...
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bioRxiv - Microbiology 2023Quote: ... and a YFP gene was amplified from the plasmid pSYFP2-C1 (generous gift from Dorus Gadella (Addgene plasmid # 22878) using primers P17/18 ...
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bioRxiv - Molecular Biology 2023Quote: shRNA oligonucleotides directed against the indicated genes were annealed and cloned into Tet-pLKO-puro lentiviral vector (Addgene #21915) digested with EcoRI and AgeI ...
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bioRxiv - Neuroscience 2023Quote: ... A virus lacking the hM4Di DREADD gene and only containing the green fluorescent tag eGFP (AAV8-CaMKIIa-eGFP, Addgene) was also infused bilaterally into either BLA (n=7) ...