Labshake search
Citations for Addgene :
501 - 550 of 908 citations for 5M Betaine Solution PCR Grade since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... the FRT-stop-FRT cassette (
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bioRxiv - Neuroscience 2020Quote: ... mScarlet-i cDNA was amplified by PCR from a pmScarlet-i_C1 plasmid (gift from Dorus Gadella, Addgene #85044) (Bindels et al. ...
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bioRxiv - Genetics 2022Quote: ... we transformed BY with a DNA fragment created by PCR amplifying the NatMX cassette from plasmid from Addgene plasmid #35121 (a gift from John McCusker ...
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bioRxiv - Microbiology 2022Quote: The Ca2+ FRET reporter Twitch-2B was PCR amplified from the plasmid Twitch-2B pRSETB obtained from Addgene [23] using the primers T2B1 and T2B2 (Table 1 ...
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bioRxiv - Immunology 2022Quote: ... The MSCV-N EBNA3A plasmid used as a backbone for PCR was a gift from Karl Munger (Addgene plasmid # 37956 ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR product was cloned into pICSL01005 (Level 0 acceptor for CDS no stop modules, Addgene no.47996) as a level 0 module for Golden Gate assembly ...
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bioRxiv - Neuroscience 2020Quote: Primers containing linkers attached to each target without the PAM sequence were used for PCR with pCFD4 (RRID:Addgene_49411) as a template ...
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bioRxiv - Neuroscience 2021Quote: Tetanus toxin light chain (TeTxLC) was amplified by PCR from pGEMTEZ-TeTxLC (Addgene #32640, (Yu et al., 2004)) using forward primer ...
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bioRxiv - Neuroscience 2021Quote: ... hM3Dq-mCherry cDNA was polymerase chain reaction (PCR)-amplified from pAAV-hSyn-hM3D(Gq)-mCherry (Addgene plasmid #50474) and transferred to the lentiviral vector CSIV with the CAG promoter ...
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bioRxiv - Immunology 2022Quote: ... a Cas9-p2a-EGFP construct was PCR amplified from the Lenti-Cas9-EGFP plasmid obtained from Addgene (63592). Then ...
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bioRxiv - Immunology 2022Quote: ... The MSCV-N EBNA3A plasmid used as a backbone for PCR was a gift from Karl Munger (Addgene plasmid # 37956; http://n2t.net/addgene:37956.; RRID:Addgene_37956) (Rozenblatt-Rosen et al. ...
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bioRxiv - Synthetic Biology 2021Quote: ... CA) or amplified by PCR with overhangs containing BpiI (BbsI) recognition sites and assembled into pUAP1 (Addgene #63674) to create parts compatible with the phytobrick standard (3) ...
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Using optogenetics to link myosin patterns to contractile cell behaviors during convergent extensionbioRxiv - Developmental Biology 2021Quote: ... the full RhoGEF2 cDNA of Drosophila melanogaster was amplified by PCR from plasmid UAS-akkordion dNhe (Addgene 41976) (71) ...
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bioRxiv - Cell Biology 2020Quote: ... the cDNA of human CAV1 was PCR amplified from the mammalian expression plasmid Emerald-CAV1 C-10 (Addgene No ...
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bioRxiv - Molecular Biology 2021Quote: ... and miRFP2 genes were PCR amplified and swapped with mClover2 in pmClover2-tubulin-C-18 plasmid (Addgene #56376) and with TagRFP675 in pTagRFP675-actin-C1 (Addgene #44277 ...
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bioRxiv - Immunology 2020Quote: Soluble human ACE2 with an Fc tag was constructed by PCR amplifying ACE2 (residues 1-615) from Addgene plasmid #1786 (a kind gift from Jesse Bloom ...
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bioRxiv - Molecular Biology 2023Quote: The DNA fragment encoding dLwaCas13a was PCR-amplified using pC035-dLwaCas13a-msfGFP (a gift from Feng Zhang; Addgene plasmid #91925 ...
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bioRxiv - Plant Biology 2023Quote: ... Each gRNA PCR fragment was individually assembled with Arabidopsis U6 small RNA promoter (pICSL01009::AtU6p, AddGene no. 46968) in the appropriate Level 1 vector (plCH47751 ...
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bioRxiv - Cell Biology 2023Quote: A PIP-FUCCI coded DNA fragment was PCR amplified with template DNA (pENTR-PIP-FUCCI; Addgene; plasmid #118621), which was a gift from Jean Cook ...
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bioRxiv - Cell Biology 2023Quote: ... were created via PCR amplification (see primer list) and assembled into a Spe-1 digested pCFJ151 (Addgene #19330) vector using isothermal assembly (Gibson et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... mRFP with a C-terminal KDEL sequence (ER-mRFP) was PCR amplified out of ER-mRFP (Addgene, 62236) using the forward primer 5’-GGTCGGATCCGCCGCCACCATGGACAGCAAAGG-3’ and the reverse primer 5’-GAGGCACCGGTGTTTAGAGCTCATCTTT-3’ ...
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bioRxiv - Synthetic Biology 2022Quote: ... we transfected 1μg of PCR-amplified dsDNA reporter into naïve mESCs together with spCas9 plasmid pX459 (Addgene #62988), carrying a single gRNA complementary to the genomic integration site ...
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bioRxiv - Microbiology 2022Quote: ... PCR fragments were cloned into the expression vector pOPINE (gift from Ray Owens; plasmid #26043, Addgene, Watertown, USA) to generate constructs ...
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bioRxiv - Cell Biology 2022Quote: Plasmids encoding GFP-2A-ORF6 were generated by inserting EGFP-T2A sequence (copied from Addgene #140424 by PCR) at the 5’ end of the ORF6 gene in pSecTag2 mammalian expression vector using Gibson Assembly ...
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bioRxiv - Cell Biology 2024Quote: The AAVS1 destination vector was assembled by PCR amplification and Gibson assembly from the pDTA-TK from Robert Benezra’s group (RRID:Addgene_22677) (Nam and Benezra 2009 ...
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bioRxiv - Molecular Biology 2024Quote: The full length BRD4 DNA fragment was amplified by PCR from pcDNA4-TO-HA-BRD4FL (Addgene plasmid #31351)61 incorporated into linearized FM5 lentiviral vectors containing standardized linkers (generously provided by David Sanders ...
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Arc mediates intercellular synaptic plasticity via IRSp53-dependent extracellular vesicle biogenesisbioRxiv - Neuroscience 2024Quote: ... This promoter sequence was PCR amplified with Mfe1 and Not1 and inserted into the pUB_smHA_KDM5B_MS2 (Addgene plasmid # 81085) lentiviral vector29 replacing the UbC promoter ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... The resultant PCR products were subcloned into BamHI- and SspI-digested pET-His6-Sumo-TEV-LIC (Addgene # 29659) using in-fusion cloning kit (Takara ...
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bioRxiv - Molecular Biology 2023Quote: ... The TIS11B coding sequence was amplified from pcDNA3.1-puro-GFP-TIS11B using TIS11B MCP F and TIS11B MCP R primers and the TIAL1 coding sequence was PCR amplified from pFRT_TO_FlagHA_TIAL1 (Addgene 106090) using TIAL1 MCP F and TIAL1 MCP R primers.
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... PCR was used to amplify the T2A-EGFP sequence from pXR001 (Addgene #109049; a gift from Patrick Hsu44) using the primers T2A-GFP Gibson Forward and Reverse (Supplementary Table 1) ...
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bioRxiv - Cell Biology 2023Quote: ... Hsp90-GFP was first constructed by PCR amplifying HA-Hsp90 (a gift from William Sessa, Addgene plasmid #22487) and recombining into GFP pBabe puro digested with EcoRI-XhoI ...
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bioRxiv - Synthetic Biology 2023Quote: ... The DNA fragments of TPPP FL and FKBP were produced by PCR using existing templates and (Addgene #31184). The tagBFP gene was synthesized as gBlocks (Integrated DNA Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... SA-KDEL-IRES and SBP PCR fragments were separately amplified from Str-KDEL_SBP-EGFP-GPI (gift from Franck Perez, Addgene plasmid #65294; RRID:Addgene_65294)28 ...
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bioRxiv - Cell Biology 2022Quote: ... sfGFP was PCR amplified from sfGFP-C1 (gift from Michael Davidson and Geoffrey Waldo, Addgene plasmid #54579; RRID:Addgene_54579)65 ...
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bioRxiv - Cell Biology 2022Quote: ... sfGFP was PCR amplified from sfGFP-C1 (gift from Michael Davidson and Geoffrey Waldo, Addgene plasmid #54579; RRID:Addgene_54579)65 ...
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bioRxiv - Genetics 2022Quote: ... The PCR product was cloned via Gibson assembly into AgeI and EcoRI digested pLKO.1 vector (Addgene#8453). Ligated libraries were electroporated into DH5a electrocompetent cells (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... the Adamts2 or TGFβR2 coding sequence was amplified by PCR using mouse Adamts2 and TGFβR2 ORF plasmids (pCMV-Adamts2, Harvard plasmid clones; pCMV-TGFβR2, Addgene) as templates ...
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bioRxiv - Cell Biology 2023Quote: ... with I532A mutation and RRRG degron) was amplified by PCR from pBMN-HA-YFP-LOV24 (Addgene, Plasmid #49570) (78 ...
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bioRxiv - Developmental Biology 2023Quote: The T7 promoter sequence was added to the Cas9 coding region by PCR amplification of px260 (Addgene, 42229) using the primer pair listed in Table S3 ...
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bioRxiv - Cell Biology 2023Quote: ... The coding sequence of full-length synaptopodin was generated by PCR and subcloned into the pKH3 vector (Addgene). IP assays were performed basically according to the method described previously (59 ...
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bioRxiv - Plant Biology 2024Quote: ... the CDS of AtPHF1 and AtPHO1 were amplified by PCR and subcloned into UBQ10:sXVE: S10-(MCS) (Addgene plasmid # 108177 ...
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bioRxiv - Plant Biology 2024Quote: ... the CDS of AtPHT1;1 and AtPHO2 were amplified by PCR and subcloned into UBQ10:sXVE: (MCS)-S11 (Addgene plasmid # 108179 ...
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bioRxiv - Plant Biology 2024Quote: ... the corresponding cDNA was amplified by PCR and cloned into the expression vector pDEST-HisMBP (Addgene plasmid #11085) using standard GATEWAYTM procedures ...
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bioRxiv - Cell Biology 2024Quote: ... IST1-EGFP was generated by PCR amplifying IST1 from HA-IST1 (Addgene plasmid # 131619 ; http://n2t.net/addgene:131619 ; RRID:Addgene_131619) and subcloning into the EGFP-N1 (Clontech ...
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bioRxiv - Molecular Biology 2024Quote: ... Kozak sequence was added to RCaMP1h by PCR using RCaMP1h cDNA from pRSET-RCaMP1h plasmid (Addgene plasmid #42874) as the template ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA encoding full-length hRhoA-DN (RhoA-T19N) was amplified by PCR from pSLIK/RhoA-T19N (# 84646, Addgene) using the following primers ...
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bioRxiv - Molecular Biology 2024Quote: ... and a nine amino acid flexible linker was included upstream of the T2A peptide sequence to facilitate efficient co-translational cleavage (34) The mScarlet (52) gene was PCR amplified from pCytERM_mScarlet_N1 (Addgene_85066) and inserted into N-terminal and C-terminal “Empty T2A::DrugR cassettes as described in the Fig ...
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bioRxiv - Plant Biology 2024Quote: PmARF1 and SmARF3 DBD sequences were introduced into PCR-amplified pET His6 MBP TEV LIC plasmid (Addgene #29656) via HiFi DNA reaction (see Supplementary Table 4 for details) ...
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bioRxiv - Immunology 2024Quote: Ubi-RdRp-EGFPOVA was generated by PCR of the ubiquitin sequence from pRK-HA-Ubiquitin-WT (Addgene, #17608) which was inserted at the N-terminus of RdRp-EGFPOVA using SacII/EcoRI ...
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bioRxiv - Neuroscience 2024Quote: ... with PCR of the hDlxI56i enhancer and minimal beta globin promoter from hDlxI56i-minBglobin-iCre-4X2C (Addgene #164450). hDlxI56i eGFP plasmid was made by cutting CMV-eGFP-synapsin (Chi et al. ...