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hsa mir 30b RT PCR Primer Set
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Cohesion Biosciences Sponsored
Stem-loop RT primer and PCR primer set to detect mature miRNA hsa-mir-30b expression.Cat# CPK3045, 1 nmol USD $100.0 Ask
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Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Genetics 2020Quote: ... hsa-mir-423-5p (Thermofisher) served as endogenous control for analysis of miRNA expression ... -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020, published in Molecular Cell doi: 10.1016/j.molcel.2020.04.030Quote: ... and miR-324 inhibitors (hsa-miR-324-5p and hsa-miR-324-3p miRCURY LNA miRNA Inhibitor) were obtained from QIAGEN. The sequences of synthetic siRNAs and miRNAs are listed in Supplementary Table S1. -
Millipore Sigma
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2022Quote: ... PCR product and pET-30b (+) (Novagen) were digested with XbaI and XhoI restriction enzymes ... -
Bio-Rad
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2018Quote: ... Prime PCR primer sets were purchased from Bio-Rad. Expression results were calculated by ΔΔCT method and were normalized to the reference genes GAPDH and βActin. -
New England Biolabs
No products found because this supplier's products are not listed.Cited in CopR, a global regulator of transcription to maintain copper homeostasis in Pyrococcus furiosusbioRxiv - Microbiology 2020Quote: ... The PCR product was cloned into vector pET-30b (NEB) using the respective restriction sites ... -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... real-time RT-PCR was performed with SARS-CoV-2 N primer sets and SYBR Premix Ex Taq II (TaKaRa-Bio) using a LightCycler Nano (Roche ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2017, published in Disease Models & Mechanisms doi: 10.1242/dmm.031716Quote: ... RT-PCR performed using specific primers sets (Supplementary Tables 2, 3) and GoTaq® Green Master Mix (Promega, Madison, WI). (30-40 cycles ... -
Illumina
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2019, published in npj Systems Biology and Applications doi: 10.1038/s41540-019-0121-4Quote: ... and indexed with ScriptSeq™ Index PCR primers set 1 (Epicentre, Illumina) following the standard protocol ... -
American BioInnovations,
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... hsa-miR-125b-5p Pre-miR™ miRNA Precursor (#AM17100, PM10148, ABI), miR-124-3p ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2022Quote: ... Primer sets were designed using ProbeFinder (Roche) and Primer 3 softwares and can be seen as Supporting Information (Table S1) ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020Quote: ... Mycoplasma Plus PCR Primer Set (Agilent) was used to determine mycoplasma for all cell lines ... -
Bioline
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... RT-PCR reactions were set up in a 384-well format using 2X SensiFAST Probe No-ROX Kit (Bioline, BIO-86005) and 1 µl of cDNA per reaction in a total volume of 10 µl ... -
Eurogentec
No products found because this supplier's products are not listed.Cited in Isolation of nucleic acids from low biomass samples: detection and removal of sRNA contaminantsbioRxiv - Molecular Biology 2018, published in BMC Biology doi: 10.1186/s12915-018-0522-7Quote: ... the aqueous phase was spiked with synthetic hsa-miR-486-3p RNA (Eurogentec) to yield 40,000 copies per μl eluate ... -
Lucigen
No products found because this supplier's products are not listed.bioRxiv - Systems Biology 2019, published in npj Systems Biology and Applications doi: 10.1038/s41540-019-0121-4Quote: ... and indexed with ScriptSeq™ Index PCR primers set 1 (Epicentre, Illumina) following the standard protocol ... -
Addgene
No products found because this supplier's products are not listed.Cited in GO-CRISPR: a highly controlled workflow to discover gene essentiality in loss-of-function screensbioRxiv - Systems Biology 2020Quote: ... was used to clone a pool of four sgRNAs per gene (AGPS, SLC2A11, ZC3H7A, PDCD2L, NPM1, EPS15, hsa-mir-761, RPAP1, SYAP1, TRAF3IP1, and EGFP) into lentiCRISPR v2 (Addgene #52961). iOvCa147 cells were transduced with viral particles encoding a Cas9 and sgRNA expression cassettes ... -
Beckman
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 E7335S, Index Primers Set 2, E7500S) with Agencourt AMPure XP magnetic beads (Beckman coulter A63881) exactly as per manufacturer’s instructions ... -
Mirus Bio
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2022Quote: Murine neonatal cardiomyocytes and NHDFs were transfected with miR-146a-5p, miR-126-3p and miR-370-3p mimics (Lightswitch, 100nM final concentration) using Trans-IT TKO (Mirus) in their respective serum free growth media ... -
GeneCopoeia
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2021Quote: ... MiRNA inhibition in MCF-10A cells was achieved by transducing cells with a miRNA inhibitor against hsa-miR-424-5p and hsa-miR-503-5p (Genecopoeia #HmiR-AN0494-AM03 and #HmiR-AN0550-AM03 respectively) ... -
Origene Technologies
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018Quote: ... An expression vector for hsa-miR-10b (Cat# SC400025, OriGene Technologies Inc., Rockville, MD) or a control pCMVMIR vector (OriGene Technologies Inc. ... -
Eppendorf
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... RT PCR was performed using the RealPlex2 (Eppendorf). Expression was normalized to ß-Actin (Mm02619580_g1 ... -
Applied Biological Materials
No products foundbioRxiv - Cell Biology 2020Quote: ... the LentimiRa-GFP-hsa-miR-494-3p Vector (Cat: mh10739, Applied Biological Materials, abmgood.com) and pLenti-III-miR-GFP Control Vector (Cat ... -
Zymo Research
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2021Quote: ... The primer sets used were Quick-16S™ Primer Set V3-V4 (Zymo Research). The sequencing library was prepared using an innovative library preparation process in which PCR reactions were performed in real-time PCR machines to control cycles and therefore limit PCR chimera formation ... -
Research Products International
No products found because this supplier's products are not listed.bioRxiv - Genomics 2018Quote: ... separate PCR reactions were set up to generate the ADT library (SI-PCR and RPI-x primers), the hashtag library (SI-PCR and D7xx_s ... -
The Jackson Laboratory
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2022Quote: ... Genotypes were confirmed using primer sets recommended by Jackson Laboratory and Sinha lab. -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Immunology 2022Quote: ... FITC-CD24 (HSA, M1/69, BD Biosciences), and the LIVE/DEAD Zombie Aqua™ Fixable Viability Kit (Biolegend) ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018Quote: ... Reactivation of the integrated HIV reporter construct was then assessed by staining for murine HSA with an anti-HSA-PE antibody (Biolegend) at 1:100 for 30 minutes ... -
Evrogen
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2017, published in PLOS ONE doi: 10.1371/journal.pone.0184507Quote: Oligonucleotide primers for DOP-PCR and iDOP-PCR were synthesized by Evrogen JSC (Moscow ... -
Active Motif
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2020Quote: ... Mouse positive control primer set Actb2 (#71017, Active Motif) and mouse negative control primer set 1 (#71011 ... -
IDT DNA
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... these were run as two separate multiplex PCR “pools” (A & B) using the ARTIC version 3 primer set (ARTIC nCoV-2019 V3 Panel, IDT DNA Inc ... -
GenScript
No products found because this supplier's products are not listed.Cited in Optogenetic actuator/ERK biosensor circuits identify MAPK network nodes that shape ERK dynamicsbioRxiv - Systems Biology 2022Quote: ... The following primers were used for the RT-qPCR reaction (designed with the Real-time PCR (TaqMan) Primer and Probes Design Tool from GenScript). -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Physiology 2019, published in Aging Cell doi: 10.1111/acel.13140Quote: ... with 100nM miR scrambled or miR-181a mimic (100nM; GE Healthcare) (Soriano-Arroquia et al. ... -
Macherey-Nagel GmbH
No products found because this supplier's products are not listed.bioRxiv - Plant Biology 2021Quote: ... RT-PCR products were purified using PCR Clean-up (Macherey-Nagel, 740609.250) following the manufacturer’s instructions ... -
Merck
No products found because this supplier's products are not listed.Cited in OR1D2 receptor mediates bourgeonal-induced human CatSper activation in a G-protein dependent mannerbioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation ... -
TianGen Biotech
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... Reverse transcription with ZIKA virus specific primer (Rev-AAGTGATCCATGTGATCAGTTGATCC) was performed using FastQuant RT Kit (Tiangen). Real-time PCR was done on 7900HT (ABI ... -
Sanquin
No products found because this supplier's products are not listed.Cited in Staphylococcal superantigen-like protein 13 activates neutrophils via Formyl Peptide Receptor 2bioRxiv - Microbiology 2018, published in Cellular Microbiology doi: 10.1111/cmi.12941Quote: ... containing 0.05% HSA (Sanquin). HL-60 cells were purchased from ATCC ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Synthetic Biology 2020Quote: ... RT-PCR was performed with RedTaq Polymerase (VWR) with primers 51-Scorpine-F and 117-CP-ctrl-R on Sco-CP (1267bp) ... -
Calbiochem
No products found because this supplier's products are not listed.Cited in A role for RNA and DNA:RNA hybrids in the modulation of DNA repair by homologous recombinationbioRxiv - Cell Biology 2018Quote: ... protease inhibitor cocktail set III (Calbiochem) and Benzonase 1:1000 (Sigma ... -
System Biosciences
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: To silence miR-424 the GFP tagged lentiviral vectors that express antisense hsa-miR-424 (MZIP424-PA-1) and corresponding scramble control lentiviral vectors (MZIP000-PA-1) were purchased from SBI (System Biosciences). Transfection of 293T packaging cell line was performed using calcium-phosphate protocol and infection of target cells was done as previously described (Platzer et al. ... -
Santa Cruz
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2019Quote: ... MART1and PFK transcripts were analyzed by SYBR Green–based real-time quantitative RT-PCR (qRT-PCR) using specific primers purchased from Santa Cruz. Data were normalized to the expression of RPL19 and are expressed as fold changes. -
Primerdesign
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Nature Communications doi: 10.1038/s41467-019-12295-2Quote: ... and using DENV RT primer/probe Mix kit (Genesig, Primerdesign Ltd., UK) according to the manufacturer’s protocol ... -
Biosearch Technologies
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: ... Two allele specific forward primers and a reverse primer were designed for use in fluorescence based competitive allele-specific PCR assays (KASP; LGC Biosearch Technologies). DNA from the 286 lines was assayed using these primers and KASP reaction mix (LGC Biosearch Technologies ... -
Vector Labs
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2019, published in Scientific Reports doi: 10.1038/s41598-020-60269-yQuote: ... the cells were incubated with DAPI diluted in PBS for 10 min at RT and washed before being mounted onto slides with Vectashield Hard Set (Vector Laboratories). Single-planed images were taken on a Nikon C2 confocal microscope using a 40X oil-immersion lens ... -
Olympus
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2020Quote: ... Filter sets from Olympus and Chroma Technology Corp (49309 ET – Orange#2 FISH and 49310 ET – Red#2 FISH ... -
Nikon
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2022Quote: ... and corresponding filter sets (Nikon). Imaging conditions were optimized for each sample ... -
Carl Zeiss
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2022Quote: ... Filter set 10 (Carl Zeiss) was used for separating the fluorescence and the excitation light ... -
Leica
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2019Quote: ... equipped with a long-pass GFP filter set (Leica filter set ET GFP M205FA/M165FC), 1.63X Plan Apo objective ... -
Chroma Technology
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2017, published in ACS Nano doi: 10.1021/acsnano.7b04743Quote: ... Fluorescence filter sets from Chroma Technology and Semrock were used ... -
Biotium Inc.
No products found because this supplier's products are not listed.bioRxiv - Pathology 2021Quote: ... Positive bands of appropriate size were confirmed using individual primer sets and resultant PCR products were visualized on a 1.5% agarose gel with gel red (Biotium). -
abcam
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2021Quote: ... Cy5-labeled aptamer-chimeras were pre-complexed with HSA (Abcam, ab8030) at a 1:1 molar ratio for 30 minutes at room temperature and then added to the HUVECs in serum-free Opti-MEM media ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019Quote: ... The RT-PCR were performed using AmpliTaq Gold DNA polymerase (Perkin Elmer, Boston, USA). PCR fragments were separated with electrophoresis using 1% agarose gel in tris-borate-EDTA (TBE ...