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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for hsa mir 30b RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... and primers specific for mmu-miR-147-3p (alias, hsa-miR-147b; Applied Biosystems). The relative abundance of EV miRNA was determined by TaqMan PCR using TaqMan Universal PCR Master Mix II and TaqMan primers and probes from Applied Biosystems ...
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bioRxiv - Genetics 2020Quote: ... hsa-mir-423-5p (Thermofisher) served as endogenous control for analysis of miRNA expression ...
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bioRxiv - Biochemistry 2023Quote: ... with specific miR inhibitors (MH10203 for hsa-miR-22-3p and MH13382 for hsa-miR-1220-3p; Thermo Scientific) using Lipofectamine RNAiMax ...
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bioRxiv - Cancer Biology 2021Quote: ... as previously described (48) using the following primers: hsa-miR-21 (Thermofisher #0003970) and Z30 (Thermofisher #001092) ...
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bioRxiv - Cell Biology 2022Quote: ... for miR-375 was quantified by TaqMan Assays using the TaqMan MicroRNA Reverse Transcription Kit and hsa-miR-375 primer sets (Thermo Fisher Scientific, 000564). MiR-375 levels were normalized to miR-U6 expression ...
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bioRxiv - Genetics 2020Quote: ... hsa-miR-147b-3p and hsa-miR-210-3p levels were assessed by TaqMan Advanced miRNA Assays (Thermofisher) and TaqMan Fast Advanced miRNA master mix (Thermofisher) ...
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bioRxiv - Neuroscience 2023Quote: ... hsa-miR-221 (Thermo Fisher Scientific #000524) and hsa-miR-182 (Thermo Fisher Scientific #002334) ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-hsa-miR-Mir9-5p (Ambion, #17000), and Anti-miR negative control (Ambion ...
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bioRxiv - Cancer Biology 2021Quote: ... Q-RT-PCR was performed using TaqMan primers/probe sets (Applied Biosystems) and analyzed as transcript copies per 50 ng RNA expressed relative to RNAPII expression as previously described (33).
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bioRxiv - Genomics 2024Quote: ... and miR-1 (Life Technologies, assay name: hsa-miR-1, cat# 4427975) following the manufacturer’s recommendations (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... Probes for TaqManTM MicroRNA Assay (hsa-miR-424-3p, hsa-miR-424-5p, U6 snRNA control) were purchased from ThermoFisher. All the steps were performed according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... and hsa-miR-182 (Thermo Fisher Scientific #002334). Relative expression of miRNAs analyzed in triplicates were normalized to the levels of Z30 and expressed as 2−ΔΔCt.
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bioRxiv - Cell Biology 2023Quote: ... Quantitative RT-PCR was performed using Taqman probes and primer sets (Applied Biosystems) specific for CD36 (assay ID Mm00432398_m1) ...
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bioRxiv - Neuroscience 2024Quote: ... Primers used were: rno-miR-155-5p (RT:002571, ThermoFisher Scientific), and rno-miR-674-3p (RT:001956 ...
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bioRxiv - Neuroscience 2022Quote: ... hsa-miR-10 (Applied Biosystems miRNA assay ID 000387), hsa-miR-129 (Applied Biosystems miRNA assay ID 000590 ...
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bioRxiv - Neuroscience 2022Quote: ... hsa-miR-129 (Applied Biosystems miRNA assay ID 000590) and hsa-miR-132 (Applied Biosystems miRNA assay ID 000457).
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bioRxiv - Genomics 2022Quote: ... and hsa-miR-152 (Life Technologies, Assay ID: 000475). Gene expression was assayed with use of the following probes ...
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bioRxiv - Neuroscience 2023Quote: ... clones were hsa-miR-21 (Thermo Fisher Scientific, #0003970), Let-7a (Thermo Fisher Scientific # 000377) ...
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bioRxiv - Molecular Biology 2021Quote: ... hsa-miR-25-3p or cel-miR-67 as control (25mM) using Oligofectamine (Invitrogen). Firefly and Renilla luciferase activities were measured using the Dual Luciferase Reporter Assay System (Promega) ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218 (Ambion, Inc., Austin, TX), or 100 nM FlexiTube siRNA to ARF6 (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), or 100 nM FlexiTube siRNA to ARF6 (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), or 100 nM FlexiTube siRNA to ARF6 (Qiagen ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with hsa-miR-3p mimic (Invitrogen) or a control mimic at 5 nM final concentration using Opti-MEM (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... or hsa-miR-106b-3p mirVana™ miRNA Mimic (Ambion) at final concentration of 5 nM ...
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bioRxiv - Neuroscience 2022Quote: ... and hsa-miR-132 (Applied Biosystems miRNA assay ID 000457).
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bioRxiv - Molecular Biology 2021Quote: ... hsa-miR-25-3p or cel-miR-67 as control (25mM) using (Lipofectamine RNAiMAX, Life Technologies). Twenty-four hours after transfection ...
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bioRxiv - Physiology 2020Quote: ... The multiplex RT primer pool consisted of primers for miR-150-5p and U6 (Thermo Fisher Scientific). No-template samples where included as negative controls.
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bioRxiv - Neuroscience 2021Quote: ... The probes hsa-miR-499a-5p (Alexa Fluor 546; Thermo Fisher) and CamK2 (Alexa Fluor 488 ...
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bioRxiv - Molecular Biology 2019Quote: ... 10-nM mirVana hsa-miR-21 specific inhibitor (Thermo Fisher Scientific), or mirVana miRNA inhibitor Negative Control (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... 18S rRNA endogenous control primer/probe set was utilized for semi-quantitative RT-PCR normalization (ThermoFisher, 4333760T). Thermal cycling conditions were adapted from Verso 1-step RT-qPCR kit (ThermoFisher ...
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bioRxiv - Molecular Biology 2024Quote: ... 20nM hsa-miR-129-5p miRIDIAN mimics (miR-129 mimics) or mimic negative control (mimics ctrl)(ThermoFisher Scientific); 20nM has-miR-129-5p miRIDIAN hairpin inhibitor (miR-129 inhibitor ...
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bioRxiv - Cancer Biology 2020Quote: ... the cells were transfected with Anti-hsa-mir-7704 inhibitor AM29132 (ThermoFisher) according to manufacturer’s instructions at 100 nM for 48 hr ...
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bioRxiv - Cell Biology 2020Quote: ... hsa-miR-494-3p inhibitor (MIMAT002816) (Cat 4464084, Assay ID MH12409, Ambion). Negative Control LNA mimic (Cat ...
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bioRxiv - Cell Biology 2020Quote: ... hsa-miR-494-3p mimic (MIMAT002816) (Cat 4404066, Assay ID MC12409, Ambion), mirVana miR inhibitor negative control #1 (Cat 4464076 ...
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bioRxiv - Genomics 2023Quote: ... TaqMan™ MicroRNA Assay hsa-miR-124-3p (ThermoFisher, AssayID 003188_mat, 4440886), TaqMan™ MicroRNA Control Assay U6 snRNA (ThermoFisher ...
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bioRxiv - Genomics 2023Quote: ... TaqMan™ MicroRNA Assay hsa-miR-423-5p (ThermoFisher, AssayID 002340, 4427975), and TaqMan™ Fast Advanced Master Mix (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 U Ribolock (40U/μl), 1x TaqMan RT primer per each gene of interest (miR-7a, miR-671, Let-7a, snoRNA202, U6 snRNA) (Applied Biosystems) and 1x first-strand synthesis buffer were filled up to 20 μl reaction volume with ddH2O and mixed ...
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bioRxiv - Molecular Biology 2020Quote: ... or hsa-miR-181-5p) (Bioneer) or with a Pre-miR miRNA Negative Control (Bioneer) using Lipofectamine 3000 (Invitrogen).
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bioRxiv - Pathology 2020Quote: ... Assay ID 245306_mat; 4427975, hsa-mir-23a-3p, Assay ID 000399; 4427975, cel-miR-39-3p, Assay ID 000200, Thermofisher). The relative expression of individual miRNA was calculated as ΔCt = mean Ct miRNA-mean Ct miR-39.
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bioRxiv - Pathology 2021Quote: ... PCR primers and probe sets were obtained from Thermofisher, USA ...
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bioRxiv - Physiology 2020Quote: mirVana hsa-miR-150-5p (ID MC10070) mimic or scrambled miRNA control (Ambion) in complex with DACC lipoplex preparation (Silence Therapeutics)(Fehring et al. ...
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bioRxiv - Neuroscience 2022Quote: ... we used hsa-miR-423-5p (suggested as endogenous control by ThermoFisher, Inc) for PD ...
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bioRxiv - Cancer Biology 2021Quote: ... Two sets of primers were used: Megaplex RT Primers Human pool A and B (Thermo Fisher, USA). Together ...
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bioRxiv - Immunology 2021Quote: ... Taqman miRNA assays for the housekeeping control gene U6 snRNA (#001973) and hsa-miR-21-5p (which also recognizes mmu-miR-21a-5p) (#000397) were purchased from Thermo Fisher. qPCR was performed using Taqman Universal Master Mix II ...
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bioRxiv - Cancer Biology 2019Quote: 50-100ng of genomic DNA from edited cells in a total PCR reaction volume of 50ul were amplified in a 30 cycle PCR using a locus-specific primer set (primer set 1) and AmpliTaq Gold 360 Master Mix (Applied Biosystems) (Supplemental Table 1b) ...
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bioRxiv - Cancer Biology 2019Quote: ... 1-2μl of extracted genomic DNA in a total PCR reaction volume of 25μl were amplified in a 35 cycle PCR using a locus-specific primer set (primer set 1) and AmpliTaq Gold 360 Master Mix (Applied Biosystems) (Supplemental Table 1b) ...
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bioRxiv - Cancer Biology 2021Quote: ... with miR-92a-specific primers (Applied Biosystems). Quantitative PCR was performed using FAST SYBR green mix (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... Capan-1 and Hs 766T were transfected with 100 nM Pre-miR Precursor to hsa-miR-218-5p (Ambion, Inc., Austin, TX), 100 nM Anti-miR miRNA Inhibitor to hsa-miR-218 (Ambion ...
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bioRxiv - Plant Biology 2022Quote: Custom TaqMan® probes (including RT primers and qRT-PCR primers, Thermo Scientific) were designed using the Small RNA Assay Design Tool with the following input sequences ...
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bioRxiv - Cancer Biology 2019Quote: ... Knockout in tissue or cells post-cre was confirmed by Q-RT-PCR using primer/probe sets for CIC (Applied Biosystems) and by Western blotting with anti-Cic.