-
No products found
because this supplier's products are not listed.
Tasneem Qaqorh, et al.,
bioRxiv - Cell Biology 2023
Quote:
... followed by in situ mRNA reverse transcription using photo-caged oligodeoxynucleotides (ODNs) primers (Glen research). After first strand synthesis ...
-
No products found
because this supplier's products are not listed.
Ling Ning Lam, et al.,
bioRxiv - Microbiology 2021
Quote:
... Authentic FAME standards were prepared as a set of dilutions 0.01 – 5.0 µg ml-1 (GLC-461 standard mix, Nu-Chek Prep) spiked with US-108N ISTD for FAME quantitation ...
-
No products found
because this supplier's products are not listed.
Katie N. Kang, Joseph M. Boll,
bioRxiv - Microbiology 2022
Quote:
... overnight cultures were back diluted to OD600 0.01 and set up as triplicate biological replicas in either a 96-well plate or 24-well plate (BrandTech BRAND). A BioTek SynergyNeo2 microplate reader was used to record optical density ...
-
No products found
because this supplier's products are not listed.
Kohitij Kar, James J DiCarlo,
bioRxiv - Neuroscience 2020
Quote:
... we used an in-house set up to lower the injection needles (30-32 gauge, small Hub RN Needle; Hamilton Company) using a micro-syringe pump and controller (Micro4™ World Precision Instruments) ...
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No products found
because this supplier's products are not listed.
Cassandra Velasco, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... PCR master mixes were decontaminated with double-stranded DNAse treatment (PCR decontamination kit, Arcticzymes, Tromsø, Norway). Sterile water was processed using the same procedure as a negative control ...
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No products found
because this supplier's products are not listed.
Caitlyn M. Edwards, Tyla Dolezel, Linda Rinaman,
bioRxiv - Neuroscience 2021
Quote:
... one set of cFos-labeled tissue sections was subsequently incubated in a rabbit polyclonal antiserum against GLP1 (1:10,000; Bachem, T-4363; AB_518978), followed by biotinylated donkey anti-rabbit IgG (1:500 ...
-
No products found
because this supplier's products are not listed.
Richard B. Kennedy, et al.,
bioRxiv - Immunology 2020
Quote:
IFNα and IFNλ production by 2’3’ cGAMP-treated STING-transduced BJAB cells were measured in triplicate using commercial sandwich ELISA assay sets (IFNα: VeriKine-HS™ Human Interferon Alpha All Subtype ELISA Kit, PBL Assay Science; Piscataway ...
-
No products found
because this supplier's products are not listed.
A. Schlör, et al.,
bioRxiv - Immunology 2022
Quote:
... 1 h incubation at RT) and 1-2 μg/mL of HRP-conjugated secondary antibody (ABIN1981272, antibodies-online) in 50 μL/well supplemented with PBS/1% casein or PBS/5 % NCS for 45 min incubation at RT.
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No products found
because this supplier's products are not listed.
Regina M. Powers, et al.,
bioRxiv - Neuroscience 2022
Quote:
... and incubated with the following primary antibodies diluted in PBS for 1hr at RT: TuJ1 (1:2000 to stain primary neurons, 1:300 to stain SH-SY5Y cells, Dr. A. Frankfurter, and Neuromics MO15013), anti-Nav1 (generation described above ...
-
No products found
because this supplier's products are not listed.
A Elgheznawy, et al.,
bioRxiv - Biochemistry 2021
Quote:
... Platelets were activated with appropriately diluted agonists for 8 min at 37°C followed by 8 min at RT in the presence of saturating amounts of PE-coupled JON/A (4H5, Emfret Analytics) detecting activated αIIbβ3 integrin and FITC-coupled anti-P-selectin (Wug.E9 ...
-
No products found
because this supplier's products are not listed.
Johanna Hol Fosse, et al.,
bioRxiv - Microbiology 2023
Quote:
... Acetone-fixed cells were incubated with IgG1 against the ISAV nucleoprotein (P10, Aquatic Diagnostics Ltd, 0.4 μg/mL, 60 min, RT), washed (PBS × 3) ...
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No products found
because this supplier's products are not listed.
Linda M. Sircy, et al.,
bioRxiv - Immunology 2023
Quote:
... virus strain for 30min at room temperature (RT) prior to addition of 50μL of 1% chicken red blood cells (cRBCs) (Lampire Biological Laboratories) in 1X PBS ...
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No products found
because this supplier's products are not listed.
Hao Wu, et al.,
bioRxiv - Immunology 2023
Quote:
... cells were stained with Fixable Viability Dye eFluor 780 (eBioscience) for 10 min in PBS at RT together with an anti-FcgRII/FcgRIII antibody (clone 2.4G2; Bio X Cell) to prevent unspecific binding ...
-
No products found
because this supplier's products are not listed.
Vipul Sharma, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... amplified using Taq Polymerase 2X PCR premix (Intact Genomics, 3249) and SX primers (SX_F ...
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No products found
Anna E. Boczula, et al.,
bioRxiv - Microbiology 2021
Quote:
... all cells were centrifuged at 220 xg RT for 5 min followed by resuspension with fresh complete media and plated at 1:5 (Cell Systems cells)-1:10 (Lonza cells ...
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No products found
because this supplier's products are not listed.
Glory Nasseri, et al.,
bioRxiv - Neuroscience 2021
Quote:
... resuspended proteins were PEGylated for 1 hr at RT to label newly exposed cysteine thiols with the 5 kDa mass tag reagent (mPEG-5k, Badrilla SiteCounter™). Approximately 20 μl of each supernatant was saved as the “total input.” For immunoblot analysis ...
-
No products found
because this supplier's products are not listed.
Qiang Sun,
bioRxiv - Plant Biology 2021
Quote:
... Xylem segments were then silver-enhanced at RT for 15 min with a silver enhancement kit (BBI Solutions, UK, Batch No. 12348). After wash with DD H2O and dehydration via an ethanol series ...
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No products found
because this supplier's products are not listed.
Jan Zlamal, et al.,
bioRxiv - Immunology 2022
Quote:
... was blocked via the pretreatment of PLTs with 5 μg/mL anti-SELP humanized Ab (anti-CD62p, 15 min, RT; ProteoGenix, Schiltigheim, France). Using a modified FC approach that detects PLT-leukocyte aggregates ...
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No products found
because this supplier's products are not listed.
Rishi R. Agrawal, et al.,
bioRxiv - Neuroscience 2022
Quote:
... the slides were equilibrated at RT for 25 min and stained using the Fluoro-Jade C (FJC) Ready-to-Dilute Staining Kit (Biosensis TR-100) following manufacturer instructions ...
-
No products found
because this supplier's products are not listed.
Aliya Sharipova, et al.,
bioRxiv - Bioengineering 2022
Quote:
... the Fe with 1wt% VH precursor mixture was loaded into a custom-build consolidation die and high-pressure cold-sintered at 2.5 GPa (corresponding to 5 t for 5 mm diameter die) and RT using manual press (Carver, Wabash, IN, USA) to obtain the drug-loaded metal (Supplementary Materials ...
-
No products found
because this supplier's products are not listed.
Max F. Moescheid, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 10 µM of 5’amine C6 primers (IDT, Coralville, IA) were incubated with 1 mM Bis-PEG10-NHS ester (BroadPharm, San Diego, CA) in borate buffer (Thermo Fisher ...
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No products found
because this supplier's products are not listed.
Timothy M. Ghaly, et al.,
bioRxiv - Microbiology 2021
Quote:
... All PCRs were performed using GeneReleaser® (Bioventures, Murfreesboro, TN, USA) as previously described [32] ...
-
No products found
because this supplier's products are not listed.
Karina Villanueva-Segura, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... The PCR products were visualized with a transilluminator (UVITEC, Cambridge, UK). At the end of amplification ...
-
No products found
because this supplier's products are not listed.
Pedro Trevizan-Baú, et al.,
bioRxiv - Neuroscience 2020
Quote:
... followed by incubation in hydrogen peroxide for 20 min and incubated for 24 h at room temperature (RT) with a goat anti-CT-B antibody (1:10,000 List Biological Laboratories, CA, USA). Then ...
-
No products found
because this supplier's products are not listed.
Kevin Christian M. Gulay, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Endogenous peroxidases were quenched with 0.3% H2O2 in methanol for 15 min at RT before blocking the tissue sections with 10% normal rabbit serum (Nichirei biosciences, Tokyo, Japan) for an hour at RT and incubating with KDM2B antibody (sc-293279 ...
-
No products found
because this supplier's products are not listed.
Giuseppe Pezzotti, et al.,
bioRxiv - Microbiology 2020
Quote:
... The cells were blocked with 2% skim milk in TBS for 60 min at RT and stained with anti-SARS Coronavirus envelope (Rabbit) antibody (Dilution =1:100) (ProSci Inc., Poway, CA, USA) for 60 min at RT ...
-
No products found
because this supplier's products are not listed.
Elizabeth A. Eckenwiler, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Sections were then incubated in primary antibody for 12-18hrs at RT (Rat anti-Substance P, 1:400, EMD Millipore, Cat # Mab356; Chicken anti-TH, 1:1000, Aves Laboratories, Cat # TYH). Slices were incubated in goat or donkey Alexa Fluor secondary antibodies (1:500 ...
-
No products found
because this supplier's products are not listed.
Ji Zhang, et al.,
bioRxiv - Pharmacology and Toxicology 2020
Quote:
... and Primary Human IPF Lung Parenchymal Fibroblasts (Donor2) (BioIVT, #PCR-70-0214) were incubated at 37°C and 5% CO2 ...
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No products found
because this supplier's products are not listed.
Emily M. Mace, et al.,
bioRxiv - Immunology 2019
Quote:
... selected with puromycin and confirmed by PCR for SV40 expression (Applied Stemcell). 293T fibroblast lines were maintained in DMEM supplemented with 10% FBS ...
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No products found
because this supplier's products are not listed.
Nora Hartig, Kathrin M. Seibt, Tony Heitkam,
bioRxiv - Plant Biology 2022
Quote:
... PCR products were purified (As One International,Invisorb Spin DNA Extraction Kit) and ligation into pGEM-T plasmids (ThermoFisher Scientific ...
-
No products found
because this supplier's products are not listed.
Jennifer Hampton Hill, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... all contain 6X-His tags and were incubated with individual membrane strips or arrays at a final concentration of 0.75 μg/mL for 1 hr at RT as directed by the manufacturer (Echelon Biosciences, Inc., Salt Lake City, UT). For visualization ...
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No products found
because this supplier's products are not listed.
Paul Mollenkopf, et al.,
bioRxiv - Biophysics 2021
Quote:
... These strands were hybridized in a Professional Standard PCR Thermocycler (Core Life Sciences Inc., USA) by denaturation for 10min at 90°C and complementary base pairing for 20h between 80° C and 20° C ...
-
No products found
because this supplier's products are not listed.
Ming Zhang, et al.,
bioRxiv - Neuroscience 2021
Quote:
... we tested 5hmC level by qPCR with BisulPlus™ Loci 5mC & 5hmC Detection PCR Kit (EpiGentek, #P-1067). Briefly ...
-
No products found
because this supplier's products are not listed.
Drake Jensen, et al.,
bioRxiv - Biochemistry 2023
Quote:
... These templates underwent an additional PCR step with Klentaq LA DNA Polymerase (84) (DNA Polymerase Technology; catalog # 110), using a biotinylated primer (Integrated DNA Technologies ...
-
No products found
because this supplier's products are not listed.
Nii-Ayi Ankrah, et al.,
bioRxiv - Plant Biology 2023
Quote:
... The telomeric probe pLT11 from Arabidopsis (Richards & Ausubel 1988) was labeled with DY-415-aadUTP by PCR (Dyomics). PCR labeling was performed with standard M13 primers using the following program ...
-
No products found
because this supplier's products are not listed.
Tyree Wilson, Susana Mouriño, Angela Wilks,
bioRxiv - Microbiology 2020
Quote:
... PCR products were analyzed via agarose electrophoresis and visualized under UV light in an AlphaImager gel doc (Protein Simple). PCR amplified products were sequenced to confirm specificity.
-
No products found
because this supplier's products are not listed.
Adeyemi Temitayo Adeyemo, et al.,
bioRxiv - Epidemiology 2019
Quote:
... and template DNA of the bacteria extracted by boiling method.9 Electrophoresis of each PCR product (5μl) was carried out in 1.5% (w/v) Agarose gel (Biomatik, Ontario, Canada) in 1X Tris-Acetate-EDTA (TAE ...
-
No products found
because this supplier's products are not listed.
GAC Singer, et al.,
bioRxiv - Genomics 2019
Quote:
Filtration and DNA extraction was done in PCR clean laminar flow hoods (AirClean Systems) thoroughly decontamination with ELIMINase (Decon Labs) and 70% EtOH prior to each set of three sample replicates ...
-
No products found
because this supplier's products are not listed.
Steffan P. Jones, et al.,
bioRxiv - Neuroscience 2022
Quote:
... qRT-PCR was performed in triplicate with oligos targeting Scn1a and 18s mRNA using Titan HotTaq EvaGreen qPCR Mix 5x (BIOATLAS). Delta Ct was determined ...
-
No products found
because this supplier's products are not listed.
Adil R Wani, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 4 transgenic lines were identified through the loss of y and were PCR-tested for orientation of insertion by Bestgene. We then tested these lines for expression of TdTomato and gene disruption after conditional expression of FLP Recombinase (Figure S3A-D) ...
-
No products found
because this supplier's products are not listed.
Isik Kantarcioglu, et al.,
bioRxiv - Biophysics 2024
Quote:
... PCR amplified tolc gene was then cloned into the pSF-Oxb14 plasmid which was obtained from Oxford Genetics (OGS557, Sigma) using the NEBuilder HiFi DNA Assembly kit (New England Biolabs) ...
-
No products found
because this supplier's products are not listed.
S.G. Badman, et al.,
bioRxiv - Microbiology 2020
Quote:
Four commercially available non-volatile transport media suitable for PCR were chosen to compare against ThinPrep-PreservCyt (Hologic Inc., Marlborough, MA): Phosphate Buffered Saline (PBS ...
-
No products found
because this supplier's products are not listed.
Rebecca H Gellman, et al.,
bioRxiv - Microbiology 2023
Quote:
... Visible colonies from the initial plates were identified via colony PCR and re-plated onto BBE and LKV plates (Anaerobe Systems).
-
No products found
because this supplier's products are not listed.
Jiangshan Wang, et al.,
bioRxiv - Bioengineering 2023
Quote:
... A 30 µL of the final master mix was added to each paper pad and left inside a PCR workstation (Mystaire, MY-PCR32) to dry for 2h ...
-
No products found
because this supplier's products are not listed.
Aleksandar Vještica, et al.,
bioRxiv - Cell Biology 2019
Quote:
... The pnmt41 and pnmt81 promoters were obtained by subsequently mutating the pnmt1 using nested PCR (with mutations as reported by Basi et al., 1993). pmam1* includes 1751bp and was point mutated to remove the AfeI site.
-
No products found
because this supplier's products are not listed.
Hesam Montazeri, et al.,
bioRxiv - Genomics 2019
Quote:
... All cell lines were confirmed negative for mycoplasma infection using the PCR-based Universal Mycoplasma Detection kit (American Type Culture Collection, Manassas, VA) as previously described [42].
-
No products found
because this supplier's products are not listed.
Ian C Tobias, et al.,
bioRxiv - Genetics 2023
Quote:
... All cultures were confirmed to be free of mycoplasma contamination during routine testing with the Mycoplasma PCR Detection Kit (BioVision Inc. K1476-100).
-
No products found
because this supplier's products are not listed.
Rani Cathrine. C, Bincy Lukose, P. Rani,
bioRxiv - Neuroscience 2019
Quote:
... PCR was performed and the product was purified from the 1% agarose gel using HiYield™ Gel/PCR DNA Mini Kit (Real Biotech Corporation, Taiwan).
-
Stem-loop RT primer and PCR primer set to detect mature miRNA hsa-mir-30b expression.
Cat# CPK3045,
1 nmol USD $100.0
No citation found on bioRxiv
-
Human hsa-miR-30b-5p miRNA Agomir is a chemically modified duplex miRNA designed to mimic the...
Cat# abx880093-5NMOL,
5 nmol USD $493.0
No citation found on bioRxiv