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No products found
because this supplier's products are not listed.
Ruben Joseph Hermann, Lutz Becks,
bioRxiv - Evolutionary Biology 2022
Quote:
All IER experiments were started with 4 rotifer/ml and 3.85 * 105 algae cells/ml in tissue cultures flask (TC T-25 standard surface filter cap, Sarstedt) in 21 ml medium ...
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No products found
because this supplier's products are not listed.
Maurits Kok, et al.,
bioRxiv - Cell Biology 2021
Quote:
... the surface was consecutively functionalized with 0.5 mg/ml PLL-PEG-biotin(20%) (SuSoS AG, Switzerland), 0.2 mg/ml NeutrAvidin (Invitrogen) ...
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No products found
because this supplier's products are not listed.
Jonathan Perr, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Cell surface protein labeling was accomplished using sulfo-NHS-biotin (APExBio, no disulfide bond) as described above after which crude membrane fractions were isolated as described for the adherent cells ...
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No products found
because this supplier's products are not listed.
Denise Guerra, et al.,
bioRxiv - Immunology 2022
Quote:
... soluble avidin-tagged Gamma S protein was biotinylated with a BIrA500 biotin-ligase reaction kit according to the manufacturer’s instruction (Avidity). Biotinylated protein was then mixed with streptavidin fluorophores (AF647 ...
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No products found
because this supplier's products are not listed.
Tho Huu Nguyen, et al.,
bioRxiv - Genetics 2019
Quote:
... The surface attached cells were fixed in 4% formaldehyde (Polysciences) for 15 min ...
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No products found
because this supplier's products are not listed.
Hannah J. Gleneadie, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... we utilised male NOD/SCID/gamma (NSG) mice (Charles River) which were kept in 12 hour light and 12 hour dark cycles in individually ventilated cages ...
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No products found
because this supplier's products are not listed.
Mostafa Jarahian, et al.,
bioRxiv - Immunology 2020
Quote:
... sapiens-wt) glycoprotein from Advanced Biomart (San Gabriel, CA, USA) and the anti-Ebola surface glycoprotein [KZ52] from Absolute Antibody (Oxford, GB). The obtained EBOV-GP was expressed with a polyhistidine-tag at the C-terminus and consists of 629 amino acids ...
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No products found
because this supplier's products are not listed.
María Fernanda Gómez-Méndez, et al.,
bioRxiv - Plant Biology 2022
Quote:
... Clathrin heavy chain (CHC) antibodies were from Agrisera. Monoclonal antibodies against phosphorylated serine (pSer) ...
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No products found
because this supplier's products are not listed.
Julian J. Freen-van Heeren, et al.,
bioRxiv - Immunology 2019
Quote:
... 1*106 T cells/condition were harvested 72h after α-CD3/α-CD28 activation and transferred to DNA Lo-binding Eppendorf tubes (Eppendorf). Cells were washed once with PBS and supernatant was completely removed ...
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No products found
because this supplier's products are not listed.
Andrew R.M. Michael, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Biotin-X-NHS (Biotin-NHS, Cayman Chemical), Sulfo-NHS-LC-Biotin (Sulfo-Biotin-NHS ...
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No products found
because this supplier's products are not listed.
Idil Ulengin-Talkish, et al.,
bioRxiv - Biochemistry 2021
Quote:
... biotin incorporation was detected using fluorophore conjugated Streptavidin antibody (Licor IRDye 800CW Steptavidin, LI-COR Biosciences). Amount of ABHD17A and APT2 was probed using FLAG (1:2,500 ...
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No products found
because this supplier's products are not listed.
Jamin Liu, et al.,
bioRxiv - Microbiology 2022
Quote:
... 293T cells were transfected with arenavirus glycoprotein expression plasmids using TransIT-LT1 (Mirus Bio). Cells were transduced the following day with VSV-ΔG-GFP (Kerafast)(45 ...
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No products found
because this supplier's products are not listed.
Yiwen Zhou, et al.,
bioRxiv - Genomics 2019
Quote:
... RNA was isolated from different number of murine splenic CD3 positive T cell samples and human oocyte samples by using the Direct-zol™ RNA MiniPrep Kit (ZYMO, cat. R2050). The extracted RNA samples were dissolved into 30 µl of nuclease-free water (Qiagen ...
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No products found
because this supplier's products are not listed.
Sophie Stephenson, et al.,
bioRxiv - Immunology 2021
Quote:
... L-685,458 (gamma-secretase inhibitor, GSI) (Tocris).
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No products found
because this supplier's products are not listed.
Marissa D. Acciani, et al.,
bioRxiv - Microbiology 2020
Quote:
... and glycoprotein (G) proteins (courtesy of Dr. Michael Whitt; KeraFAST) (53) ...
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No products found
because this supplier's products are not listed.
Han Zhu, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 100 nM gamma secretase inhibitor XX (Calbiochem, 565789), 10 μg/ml heparin ...
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No products found
because this supplier's products are not listed.
Jyotirmoy Das, et al.,
bioRxiv - Genomics 2021
Quote:
The DNA methylome data of HLA-DR+/CD3- and CD3+ cells was analyzed using the HumanMethylation450K (450K) BeadChip (Illumina, USA) array as per the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Lauren E. Mehanna, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The biotin-streptavidin conjugations on the substrate surface were verified using fluorescent microscopy on an Eclipse Ti-U inverted microscope (Nikon, Minato City, Tokyo, Japan). Pattern sizes of 50 μm,100 μm ...
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No products found
because this supplier's products are not listed.
N Pollock, et al.,
bioRxiv - Physiology 2022
Quote:
... and subsequently incubated with primary antibody against DNP (Cell Biolab; 1:1000 dilution 5% FSG TBS-T) overnight at 4°C ...
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No products found
because this supplier's products are not listed.
Mirjam Pennauer, et al.,
bioRxiv - Cell Biology 2021
Quote:
... we used the surface tool of the Imaris Cell Imaging software (Oxford Instruments).
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No products found
because this supplier's products are not listed.
Jeffrey B. McMahan, John T. Ngo,
bioRxiv - Biochemistry 2022
Quote:
... Stock solutions of biotin-tetrazine (biotin-PEG4-methyltetrazine, Click Chemistry Tools), bis-biotinamide (bis-dPEG3-biotin ...
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No products found
because this supplier's products are not listed.
John E G Lawrence, et al.,
bioRxiv - Bioengineering 2023
Quote:
... and Opal 650 and TSA-biotin (TSA Plus Biotin Kit) (Akoya Biosciences) and streptavidin-conjugated Atto 425 (Sigma ...
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No products found
because this supplier's products are not listed.
Daisuke Nakane, et al.,
bioRxiv - Microbiology 2021
Quote:
Cell behaviour on the glass surface was visualized under an inverted microscope (IX83; Olympus) equipped with 10× objective lenses (UPLFLN10×2PH ...
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No products found
because this supplier's products are not listed.
Lena Le Quellec, et al.,
bioRxiv - Bioengineering 2023
Quote:
... the chips are surface treated with hydrophobic solution (NOVEC 1720 surface modifier/electronic grade coating 3M). To do so ...
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No products found
because this supplier's products are not listed.
Jerry Yan, et al.,
bioRxiv - Bioengineering 2023
Quote:
... plated at 800,000 splenocytes/well and restimulated with 10 μg rabies virus glycoprotein peptide (AnaSpec) for 24h at 37 °C ...
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No products found
because this supplier's products are not listed.
Nicolás Cano-Astorga, et al.,
bioRxiv - Neuroscience 2024
Quote:
... All surfaces of the blocks except the top surface were covered with silver paint (Electron Microscopy Sciences, USA) to prevent any charging of the resin ...
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No products found
because this supplier's products are not listed.
Rui Tang, et al.,
bioRxiv - Bioengineering 2020
Quote:
... A549 cells were co-cultured with CD4 pos T cells in glass-bottom plates (MatTek Corporation) and imaged on an LSM 700 confocal microscope (Zeiss).
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No products found
because this supplier's products are not listed.
M. Gordon Joyce, et al.,
bioRxiv - Immunology 2021
Quote:
... gamma chain specific (The Binding Site) was added and incubated at RT for an hour ...
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No products found
because this supplier's products are not listed.
Jordan A. Stinson, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... specifically positive for CD3 (T lymphocyte; Biocare CP215C), Iba-1 (macrophage ...
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No products found
because this supplier's products are not listed.
Jovana Vasiljević, et al.,
bioRxiv - Cell Biology 2021
Quote:
... where anti-mouse light chain specific HRP-conjugated antibodies (Dianova) were used to avoid interference from the heavy chain of the precipitated IgG ...
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No products found
because this supplier's products are not listed.
Cindy F. Yang, et al.,
bioRxiv - Neuroscience 2019
Quote:
Anti-somatostatin-14 antibody (Peninsula Laboratories, Cat# T-4103.0050, RRID: AB_518614) was raised in rabbit against the first 14 aa of the synthetic peptide SST ...
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No products found
because this supplier's products are not listed.
Jennifer Osten, et al.,
bioRxiv - Physiology 2022
Quote:
... The atrial regulatory light chain (MLC2a) was labelled with a MLC2a specific antibody (Synaptic Systems GmbH, 311011).
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No products found
because this supplier's products are not listed.
Kyeongseon Min, et al.,
bioRxiv - Biophysics 2024
Quote:
The rat with a cranial chamber installed on the cortical surface was placed on a 7 T MRI system (BioSpec 70/20 USR, Bruker BioSpin), fixed in a customized cradle with two ear-bars and a bite-bar ...
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No products found
because this supplier's products are not listed.
Neil Portman, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... T-47D and ZR75-1 cells (American Type Culture Collection) lines were verified through short tandem repeat profiling and tested negative for mycoplasma contamination at the commencement of the project ...
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No products found
because this supplier's products are not listed.
Alma-Martina Cepika, et al.,
bioRxiv - Immunology 2024
Quote:
... CD4+ T cells were collected and gDNA was isolated with Quick Extract (Lucigen) following manufacturer instructions ...
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No products found
because this supplier's products are not listed.
Xiaozheng Xu, et al.,
bioRxiv - Immunology 2021
Quote:
... 2% biotin-DPPE (Avanti Polar Lipids, #870285P), 2% DGS-NTA-Ni (Avanti Polar Lipids ...
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No products found
because this supplier's products are not listed.
Kosuke Hamaguchi, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The surface was covered by silicon elastomer (Kwik-Sil, WPI) to protect the craniotomy until the recording ...
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No products found
because this supplier's products are not listed.
Yanfei Ma, et al.,
bioRxiv - Plant Biology 2019
Quote:
... 50 using RFP-Trap single chain antibodies (Chromotek). Enrichment of specific DNA fragments was validated by qPCR at the ARR7 promoter region24 ...
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No products found
because this supplier's products are not listed.
Elisa Arthofer, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... T cells were electroporated in Neon Buffer T with 15μg Cas9 mRNA (TriLink) and 10μg total sgRNA (Synthego ...
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No products found
because this supplier's products are not listed.
John Sturgis, et al.,
bioRxiv - Cell Biology 2023
Quote:
... DNA Polymerase gamma Rabbit mAb (AbClonal, A1323, 1:500) followed by washing and incubation with anti-mouse IRDye®680RD ...
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No products found
because this supplier's products are not listed.
Jacob Duran, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Cells were next incubated in 500 mM biotin-phenol (AdipoGen) for the last 45 min of LLOMe incubation ...
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No products found
because this supplier's products are not listed.
Marie-Ève Lebel, et al.,
bioRxiv - Immunology 2024
Quote:
... 50 µg NP-CGG (Chicken Gamma Globulin, LGC Biosearch Technologies) precipitated in alum (Cedarlane) ...
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No products found
because this supplier's products are not listed.
Eleanor M Denham, et al.,
bioRxiv - Immunology 2019
Quote:
Cells were analysed for receptor surface expression by flow cytometry using anti-Strep-tag II antibody Oyster 645 (IBA Lifesciences # 2-1555-050), or anti-Strep-tag II antibody (IBA Lifesciences # 2-1507-001 ...
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No products found
because this supplier's products are not listed.
Immacolata Porreca, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... T cells were lysed using DirectPCR (cell) (Viagen Biotech, LA, USA) lysis buffer supplemented with proteinase K (10 µg/mL ...
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No products found
because this supplier's products are not listed.
Venetia Bazioti, et al.,
bioRxiv - Immunology 2022
Quote:
Splenic CD4+ and CD8+ T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated and labeled using Incucyte Caspase3/7 Red Reagent (Sartorius). Cells were cultured in RPMI 1640 medium (Gibco ...
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No products found
because this supplier's products are not listed.
Jessica M. Ross, et al.,
bioRxiv - Neuroscience 2023
Quote:
... using two surface electrodes (Ambu Neuroline 715) in order to approximate the somatosensory sensations arising from skin mechanoreceptors and scalp muscles during the active TMS condition [50] ...
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No products found
because this supplier's products are not listed.
Kento Ojima, et al.,
bioRxiv - Neuroscience 2021
Quote:
... iRFP 670 positive cells were selected and the maximam signal intensity of the cell surface was calculated by ZEN blue software (Carl Zeiss). The membrane intensity was fitted with KaleidaGraph (Synergy Software) ...
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No products found
because this supplier's products are not listed.
Akiko Uyeda, et al.,
bioRxiv - Neuroscience 2019
Quote:
... The membrane was incubated with the following primary antibodies diluted in 5% nonfat dry milk/TBS-T overnight at 4°C: rabbit polyclonal anti-5-hydroxymethylcytosine antibody (Active Motif, 39769) at 1:5000 and mouse monoclonal anti-5-methylcytosine antibody (Active Motif ...
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No products found
because this supplier's products are not listed.
Heidi Peussa, et al.,
bioRxiv - Bioengineering 2022
Quote:
... transmitted light (T-PMT) was used to focus the stimulation laser to the DR1-glass layer and defined regions of interest (3 x 5 rectangles á 7×400 pixels ...
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No products found
because this supplier's products are not listed.
Brandon M Bensel, et al.,
bioRxiv - Biophysics 2023
Quote:
... Liposomes were extruded through a 1 μm pore diameter extruder (T&T Scientific) and incubated overnight with 1 μM thiolated neutravidin (SH-NaV) ...