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Citations for Lucigen :
1 - 50 of 456 citations for T Cell Surface Glycoprotein CD3 Gamma Chain CD3G Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were collected and gDNA was isolated with Quick Extract (Lucigen) following manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: Genomic DNA from 104 to 105 T cells was was extracted using QuickExtract buffer (Lucigen). The resulting product was used as a DNA template for a first PCR amplification reaction using primers F2(RB198)* and R2(RB199)* primers (Supplemental Table 1) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Genomic DNA from 5,000 - 50,000 CAR-expressing T cells was extracted using Quick Extract (Lucigen) and used as the template for a 2-step PCR strategy ...
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bioRxiv - Neuroscience 2022Quote: ... Genotyping was achieved by polymerase chain reaction with EconoTaq® DNA polymerase (Lucigen). Primers for genotyping of CEP164 mice ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.75 mM biotinylated UTP (Biotin-16-UTP, Lucigen BU6105H) and either 7.5 mM GTP or 6.75 mM 7-deazaguanine (TriLink N-1044 ...
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bioRxiv - Synthetic Biology 2021Quote: ... genomic DNA was extracted from 104 to 105 CAR-expressing T cells using QuickExtract buffer (Lucigen). The resulting product was used as a DNA template for a first PCR amplification reaction using F5 and R5 primers (Supplementary Table 2 ...
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bioRxiv - Genomics 2022Quote: ... including a 1:4 ratio of biotin-16-UTP (BU6105H, Lucigen) to UTP at 5 mM ...
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bioRxiv - Molecular Biology 2021Quote: ... and T erminator 5’-Phosphate Dependent Exonuclease (Lucigen) (1 U per 5 μg of RNA ...
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bioRxiv - Biochemistry 2022Quote: ... the electroporated T cells were pelleted and gDNA was isolated by adding 50 μL/well QuickExtract DNA extraction solution (Epicentre), followed by incubation at 37 °C for 10 minutes ...
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bioRxiv - Biophysics 2022Quote: ... The WT NPSL2 DNA template was amplified via polymerase chain reaction (PCR) using a set of four primers (Table S4) with EconoTaq PLUS 2x Master Mix (Lucigen). All the primers were ordered from Integrated DNA Technologies ...
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bioRxiv - Immunology 2023Quote: ... HRV amplicons (408bp) were generated by polymerase chain reactions (PCR) using EconoTaq PLUS Green 2X Master Mix (Lucigen; 30033-1) and HRV serotype-specific primers (Fwd 5’- -3’ ...
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bioRxiv - Biophysics 2023Quote: The templates for preparation of the extended pre-miR-31 for DMS-MaPseq and FL pre-miR-31 for NMR studies were generated by overlap-extension (OE) polymerase chain reaction (PCR) using EconoTaq PLUS 2x Master Mix (Lucigen) with primers listed in Tables S6 and S7 ...
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bioRxiv - Biochemistry 2024Quote: ... GG-pre-miR-19a and pre-miR-143 were generated by overlap-extension (OE) polymerase chain reaction (PCR) using EconoTaq PLUS 2x Master Mix (Lucigen) with primers listed in Table S1 ...
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bioRxiv - Cell Biology 2019Quote: ... and in vitro transcription performed using the T7-Flash BiotinRNA Transcription Kit (Epicentre, biotin labelling) or TranscriptAid T7 High Yield Transcription Kit (ThermoScientific ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatants were used as DNA templates for polymerase chain reactions (PCRs, EconoTaq Plus Green 2X mater mix, Lucigen, Middleton, WI, USA). The genotyping primers were as described previously29,30,92
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were used as DNA templates for polymerase chain reactions (PCRs, EconoTaq Plus Green 2X mater mix, Lucigen, Middleton, WI, USA). For embryonic genotyping during primary culture preparation ...
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bioRxiv - Genomics 2020Quote: ... both transcripts were biotin-labeled after in vitro transcription from 1µg linearized pcDNA3.1-LETR1-1 and pcDNA3.1-LETR1-1-antisense plasmids for 1h at 37°C using Ampliscribe T7-flash biotin-RNA kit (Lucigen). Biotinylated LETR1 sense and antisense RNA were then treated with RNase-free DNase I for additional 15min at 37°C ...
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bioRxiv - Molecular Biology 2020Quote: ... were mounted on the slides and sections were preconditioned for 30 minutes at room temperature (R/T) with hybridization-reaction mixture of 1X Ampligase Buffer (Lucigen, A1905B), 0.05M KCl (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2023Quote: Fosmids from each hit were isolated by BioS&T (Quebec, Canada) or using the FosmidMAX™ DNA Purification Kit (Lucigen Corporation). Isolated fosmids were prepared for Illumina sequencing using the plexWell™ 96 kit (seqWell ...
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bioRxiv - Developmental Biology 2023Quote: ... coli cells (Endura electrocompetent cells, Lucigen) following the manufacturer’s instructions transforming 90 ng of library DNA into 25 μL of electrocompetent cells for each electroporation reaction for a total of 6 reactions and the cells were pooled and plated on 6 x LB agar plates (245-mm square bioassay dish ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (Lucigen) and purified as described by Urbanek et al ...
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bioRxiv - Biochemistry 2019Quote: ... coli cells (Lucigen) by electroporation ...
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bioRxiv - Biochemistry 2019Quote: ... coli cells (Lucigen) were transformed by electroporation ...
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bioRxiv - Biochemistry 2019Quote: ... coli cells (Lucigen) were transformed with the resulting plasmid by electroporation ...
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bioRxiv - Biochemistry 2019Quote: ... coli cells (Lucigen) by electroporation ...
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bioRxiv - Biochemistry 2019Quote: ... coli cells (Lucigen) by electroporation ...
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bioRxiv - Biochemistry 2021Quote: ... coli cells (Lucigen). Cells cultured in SOC media and recovered for 45 minutes at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... TG1 cells (Lucigen) were transformed with this library by electroporation.
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells (Lucigen) and then cultivated at 37°C in LB medium supplemented with kanamycin and streptomycin ...
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bioRxiv - Systems Biology 2020Quote: ... coli cells (Lucigen) using a GenePulserXCell (Biorad ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (Lucigen) were transformed with expression plasmid and a single colony was used to start an overnight culture in LB media ...
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bioRxiv - Genomics 2022Quote: ... coli cells (Lucigen) using a MicroPulser Electroporator (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells (Lucigen). To allow for fluorescent labeling of Wsp1p-PRD ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli cells (Lucigen), which were plated on LB agar supplemented with 100 µg mL−1 ampicillin ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (Lucigen) harboring the pTA-MOB conjugation helper plasmid (Strand et al. ...
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bioRxiv - Genomics 2022Quote: ... coli cells (Lucigen) using a MicroPulser Electroporator (Bio-Rad) ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells (Lucigen). Integration ...
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bioRxiv - Systems Biology 2023Quote: ... coli cells (Lucigen), and recovered for one hour at 37°C in Recovery Media (Lucigen) ...
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bioRxiv - Microbiology 2019Quote: ... cloni electrocompetent cells (Lucigen) according to manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2020Quote: ... cloni® cells (Lucigen) and plated on 37 large agar plates with Kanamycin antibiotics in order to conserve library complexity ...
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bioRxiv - Genetics 2021Quote: Endura electrocompetent cells (Lucigen) were electroporated with the genome-scale CRISPR KO (Brunello ...
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bioRxiv - Bioengineering 2021Quote: ... coli electrocompetent cells (Lucigen) then plated on a 245 × 245 mm LB and kan agar plate ...
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bioRxiv - Biochemistry 2022Quote: ... coli competent cells (Lucigen). A single colony of transformed cells was grown in 3 mL lysogeny broth (LB ...
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bioRxiv - Molecular Biology 2020Quote: C41(DE3) cells (Lucigen) were transformed with the expression plasmid using standard techniques ...
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bioRxiv - Biochemistry 2022Quote: ... cloni 10G cells (Lucigen) by electroporation (1.8 kV) ...
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bioRxiv - Bioengineering 2022Quote: ... coli SS320 cells (Lucigen) containing the circular phagemid comprising the target scaffold sequence and a M13cp helper plasmid ...
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bioRxiv - Genetics 2019Quote: ... Copy cutter cells (Epicentre) were used for propagation as the slc12a7a/kcc4a gene product was toxic to commonly used bacteria when at high copy number ...
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bioRxiv - Genetics 2022Quote: ... cloni 10G cells (Lucigen) and purified using the ZR miniprep kit (Zymo Research) ...
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bioRxiv - Plant Biology 2022Quote: ... coli C41 cells (Lucigen). KTI1 ...
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bioRxiv - Genetics 2023Quote: EPI300 bacterial cells (Lucigen) harboring the BAC clone CH322-154G8 (BACPAC Genomics ...