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No products found
because this supplier's products are not listed.
Matthew R. King, Sabine Petry,
bioRxiv - Biochemistry 2019
Quote:
80µL of a dilute solution of GMPCPP-stabilized microtubules containing 10% Alexa568-labled tubulin and 10% biotinylated-tubulin were attached via anti-biotin antibodies adhered to the surface of a blocked (κ-casein) coverslip-bottomed CultureWell (Grace BioLabs: 112359). The microscope stage was positioned and focused on MTs ...
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No products found
because this supplier's products are not listed.
Yulia Kiyan, et al.,
bioRxiv - Cell Biology 2020
Quote:
... and 10 U/ml recombinant mouse interferon gamma (Cell Sciences, Canton, MA) at 33 °C (permissive conditions) ...
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No products found
because this supplier's products are not listed.
Elissa K. Leonard, et al.,
bioRxiv - Bioengineering 2023
Quote:
... The heavy chain (HC) and light chain (LC) of the 602 antibody were separately cloned into the gWiz vector (Genlantis) (51) ...
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No products found
because this supplier's products are not listed.
Anca F. Savulescu, et al.,
bioRxiv - Cell Biology 2022
Quote:
... ± 100U/ml interferon gamma (PBL Assay Science) and incubated at 37 °C for 16-24 hours ...
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No products found
because this supplier's products are not listed.
Koushik Debnath, et al.,
bioRxiv - Bioengineering 2024
Quote:
... or biotin anti-mouse fibronectin (FN) antibody (#IRBAMSFBNGFBL; Innovative Research). 4.0 x 108 streptavidin-coated magnetic beads were used to incubate with 20 µg of the biotinylated antibody either at RT for 1 h or at 4 °C for 8 h with gentle tilting and rotating ...
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No products found
because this supplier's products are not listed.
Tetsuro Yamamoto, et al.,
bioRxiv - Immunology 2023
Quote:
... Monkey IFN-gamma ELISA Kit (U-CyTech biosciences), and Monkey IL-17 ELISA Kit (U-CyTech biosciences) ...
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No products found
because this supplier's products are not listed.
Sanne Duinkerken, et al.,
bioRxiv - Immunology 2019
Quote:
... EpCAM BV421(clone 9C4, Antibody Chain) and maturation markers CD86 FITC (clone BU63, ImmunoTools), CD80 FITC (clone 2D10 ...
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No products found
because this supplier's products are not listed.
Awatef Allouch, et al.,
bioRxiv - Immunology 2022
Quote:
... cell volumes and surfaces were measured by Volocity software (Quorum Technologies). For detection of CFSE+ and mCherry+ cells engrafted in mice tissues ...
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No products found
because this supplier's products are not listed.
Laura E. Doepker, et al.,
bioRxiv - Immunology 2020
Quote:
Antibody heavy and light chain variable regions were synthesized as FragmentGENES (GENEWIZ®, South Plainfield, NJ) and subsequently cloned into corresponding Igγ1 ...
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No products found
because this supplier's products are not listed.
Lu Lv, et al.,
bioRxiv - Biochemistry 2020
Quote:
... D-Biotin (Targetmol, T1116) was added to the transfected cells at a final concentration of 50 μM ...
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No products found
because this supplier's products are not listed.
Yaozong Chen, et al.,
bioRxiv - Microbiology 2021
Quote:
... and incubated with goat anti-guinea pig C3 antibody conjugated with biotin (Immunology Consultants Laboratory) at RT for 1 h followed by incubation with streptavidin R-Phycoerythrin (PE ...
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No products found
because this supplier's products are not listed.
NV DiBenedetto, et al.,
bioRxiv - Microbiology 2023
Quote:
... diluted at 4ng/ml in PBS was used for the capture antibodies and the T4G1 monoclonal antibodies previously coupled to biotin were used as detection antibodies (BBI solution, 1:10000 dilution) with streptavidin HRP (Thermo Fisher Scientific) ...
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No products found
because this supplier's products are not listed.
Saskia Meyer, et al.,
bioRxiv - Immunology 2021
Quote:
... and reacted successively with amine-reactive biotin (sulfo-NHS-LC-biotin, Proteochem, USA) and neutravidin (Thermo Fisher) ...
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No products found
because this supplier's products are not listed.
Fabio Palmieri, et al.,
bioRxiv - Microbiology 2020
Quote:
... in BronchiaLife™ B/T complete medium (Lifeline Cell Technology, USA) supplemented with 0.5% Phenol Red solution (Sigma-Aldrich ...
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No products found
because this supplier's products are not listed.
Rajendra Karki, et al.,
bioRxiv - Immunology 2020
Quote:
... or 500 μg of neutralizing antibody against TNF-α (Leinco Technologies, Inc., T-703) plus 500 μg of neutralizing antibody against IFN-γ (Leinco Technologies ...
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No products found
because this supplier's products are not listed.
Matthew J. Bovyn, et al.,
bioRxiv - Cell Biology 2020
Quote:
... diluted to ~20 nm was mixed with ~1 pm of penta-His-biotin antibody conjugated streptavidin polystyrene beads (0.43 μm or 0.56 μm or 0.89 μm diameter, Spherotech). This ratio produced the bead binding fraction of 10-15% and was maintained to maximize the probability of finding single motor beads in the solution ...
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No products found
because this supplier's products are not listed.
Fabrizia Zevolini, et al.,
bioRxiv - Immunology 2023
Quote:
... CTLs at 5 days of differentiation were transiently transfected using the Human T cell nucleofector kit and the program T-023 of the Nucleofector II system (Amaxa Biosystems, Euroclone, Milan, Italy) for activated cells with 1 μg/106 cells of pEGFP-EB1 plasmid ...
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No products found
because this supplier's products are not listed.
Azucena Bermejo-Nogales, José M. Navas,
bioRxiv - Zoology 2021
Quote:
... Products were sequenced by the deoxy chain termination method (ABI PRISM dRhodamine terminator cycle sequencing kit ...
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No products found
because this supplier's products are not listed.
Prabhu S. Arunachalam, et al.,
bioRxiv - Immunology 2021
Quote:
... or IgA (α-chain specific, Alpha Diagnostics, 1:4’000 dilution) in PBS-T containing 1% non-fat milk was added and incubated for 1 hour at RT ...
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No products found
because this supplier's products are not listed.
Daniela Di Girolamo, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... 3.6 ug of Sens Target DNA are fragmented and biotin labelled using Encore Biotin Module kit (Tecan). After control of fragmentation using Bioanalyzer 2100 ...
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No products found
because this supplier's products are not listed.
Magdalena Kasendra, et al.,
bioRxiv - Cell Biology 2019
Quote:
Images of microvilli on the surface of cells were captured with a scanning electron microscope (JSM-5600LV; JEOL). The morphological analysis and quantification of microvilli was performed using ImageJ ...
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No products found
because this supplier's products are not listed.
Brigette Y. Monroy, et al.,
bioRxiv - Biophysics 2019
Quote:
... or 50 mM D-biotin (CHEM-IMPEX) in lysis buffer ...
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No products found
because this supplier's products are not listed.
Eirini Tsekitsidou, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 50 μM D-biotin (Bio Basic, BB0078) was added to the media for 15 minutes at 37 °C ...
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No products found
because this supplier's products are not listed.
Naoki Iwanaga, et al.,
bioRxiv - Immunology 2020
Quote:
ELISA plates were coated with 2 μg/mL recombinant spike glycoprotein receptor binding domain (RBD) from SARS-CoV-2 (BEI RESOURCES) or recombinant S1 subunit (RayBiotech) overnight at 4 °C ...
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No products found
because this supplier's products are not listed.
Jonathan Richard, et al.,
bioRxiv - Immunology 2021
Quote:
... CD4-conjugated antibodies were produced in stable cell lines via transient transfection of plasmid coding light chains using EndoFectin™ Max transfection agent (GeneCopoeia). Transfected cells were incubated at 37°C ...
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Cell strainers (Falcon), components of the NCIS kit. Suitable for removal of tissue debris in...
Cat# LK003265,
5 ea, $50.00
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Jacob W. Freimer, et al.,
bioRxiv - Genomics 2021
Quote:
... and treated each T cell culture with 200U/mL of DNase (Worthington Cat # LS002007) for 30 mins at 37 degrees ...
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No products found
because this supplier's products are not listed.
Shipra Das, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... the cells were washed twice in Cytoporation buffer T (BTX Harvard Apparatus, Holliston, Massachusetts), and resuspended at a final concentration of 28□×□106 cells/mL in the same solution ...
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No products found
because this supplier's products are not listed.
Matthew E. Griffin, et al.,
bioRxiv - Cell Biology 2023
Quote:
Biotin-PEG4-CO2H (BroadPharm, 400 mg, 0.814 mmol) and N,N’-dimethylbarbituric acid (165 mg ...
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No products found
because this supplier's products are not listed.
José Javier Conesa, et al.,
bioRxiv - Microbiology 2020
Quote:
... 105 fluorescence-stained cells were deposited on the surface of Au-G200F1 finder grids coated with holey carbon (R 2/2; Quantifoil) and functionalized with Poly-L-Lysine (Merck ...
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No products found
because this supplier's products are not listed.
Nairi Hartooni, et al.,
bioRxiv - Biophysics 2021
Quote:
... Excess NeutrAvidin was washed away and incubated with either biotin-conjugated mouse monoclonal anti-Strep-Tag II antibody (LifeSpan BioSciences, Inc.) or biotin-conjugated rabbit polyclonal anti-GFP antibody diluted in buffer C + BSA (0.1 mg/ml Molecular Biology Grade Bovine Serum Albumin) ...
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No products found
Hui-Hsuan Kuo, et al.,
bioRxiv - Cell Biology 2019
Quote:
... thiazovivin (LC Labs, T-9753), recombinant human TGFβ3 (Cell Guidance Systems ...
-
No products found
because this supplier's products are not listed.
Brian G. Pierce, et al.,
bioRxiv - Microbiology 2020
Quote:
The interaction of recombinant sE2 glycoproteins with CD81 and HMAbs in was measured using an Octet RED96 instrument and Ni2+-NTA biosensors (Pall ForteBio). The biosensors were loaded with 5 μg/mL of purified His6-tagged wild-type or mutant sE2 for 600 sec ...
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No products found
because this supplier's products are not listed.
Paulus Mrass, et al.,
bioRxiv - Immunology 2022
Quote:
... we loaded cytotoxic T cells for 30 minutes with 4 μM Cal-520 (AAT Bioquest), and transferred 2 x 104 cells per well into flat-bottom 96 well plates coated with a CD3 antibody ...
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No products found
because this supplier's products are not listed.
Biyong Liu, et al.,
bioRxiv - Cell Biology 2024
Quote:
The BC0510 Mitochondrial Respiratory Chain Complex I Activity Assay Kit produced by Solarbio was used to detect the activity of respiratory chain complex I ...
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12 well plate with tissue culture treated #1.5 glass-like polymer cover slip (0.175±0.010mm)....
Cat# P12-1.5P,
20/case, $221.00
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Mike T. Veling, et al.,
bioRxiv - Synthetic Biology 2022
Quote:
HEK293-T cells were seeded on 96 well glass bottom plates from Cellvis (P96-1.5H-N). Cells within each well were transfected with 50 ng of the LLPS1-iLID::EGFP::FTH1 (Addgene plasmid #122149 ...
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No products found
because this supplier's products are not listed.
Costanza Borrelli, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... 96 or 384 well plates were coated with rat tail collagen with the Collagen-I Cell Culture Surface Coating Kit (ScienCell Research Laboratories) following manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Anthony Kischel, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... which was blocked for 30 min and incubated with primary antibody in 5% nonfat dry milk in TBS-T (20 mM Tris base (Cat#26-128-3094-B, Euromedex), 150 mM NaCl ...
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No products found
because this supplier's products are not listed.
Lucas Sire, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Coleoptera specimens recovered from WFTs were morphologically sorted and identified to species level by expert taxonomists while MT recovered arthropod communities were ground to fine powder using BMT-50-S-M gamma sterile tubes with 10 steel beads (IKA®-Werke GmbH & Co KG ...
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No products found
because this supplier's products are not listed.
B. Afzali, et al.,
bioRxiv - Immunology 2019
Quote:
... and used to transduce pre-activated primary human CD4+ T cells in combination with Transdux (System Biosciences). GFP+ cells were FACS sorted 48h later ...
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No products found
because this supplier's products are not listed.
Elijah A. Petter, et al.,
bioRxiv - Neuroscience 2022
Quote:
... DV: 2.0 mm from skull surface) using a microinjector (Nanoject 3000, Drummond Scientific). Optic fibers (SFLC230-10 ...
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No products found
because this supplier's products are not listed.
Emanuel J. Novais, et al.,
bioRxiv - Cell Biology 2024
Quote:
... deparaffinized sections were blocked in 5% normal serum in PBS-T and incubated with antibodies against COL2 (1:400, Fitzgerald 70R-CR008), COMP (1:200 ...
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No products found
because this supplier's products are not listed.
Alisa Fox, et al.,
bioRxiv - Immunology 2021
Quote:
... cells were washed and incubated in Accutase cell detachment solution for 10 min at room temperature to gently remove any surface-bound virus while preserving epitopes for flow cytometry analysis (Innovative Cell Technologies; as described in (39)) ...
-
No products found
because this supplier's products are not listed.
Dilara Ayyildiz, et al.,
bioRxiv - Genetics 2021
Quote:
Quantitative polymerase chain reaction (qPCR) was performed using SensiFAST™ SYBR No-ROX Kit (Bioline, BIO-98020) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Yannick O Alexandre, et al.,
bioRxiv - Immunology 2020
Quote:
Endogenous HSV and LCMV-specific CD8 T cells were identified with H-2Kb-gB498-505 and H-2Db-gp33-41 (MBL international)-restricted tetramer staining respectively.
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No products found
because this supplier's products are not listed.
Dorothee Jakob, et al.,
bioRxiv - Molecular Biology 2021
Quote:
Cells were stained for vimentin (fibroblasts, myofibroblasts, endothelial cells; antibody from Progen, Germany), CD31 (endothelial cells ...
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No products found
because this supplier's products are not listed.
Alice Mazzagatti, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... cells were incubated with primary antibodies according to suppliers’ instructions: CREST (Antibodies Incorporated, 15-234-0001), γH2aX (Millipore ...
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No products found
because this supplier's products are not listed.
Zaili Luo, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Cells were then resuspended in 100 μl cold Antibody Buffer and 1□0μl antibody (H3K4me3, EpiCypher #13-0041 ...
-
No products found
because this supplier's products are not listed.
Alok Nath Mohapatra, et al.,
bioRxiv - Neuroscience 2023
Quote:
... The EAr was lowered onto the surface of the exposed brain using a motorized manipulator (MP200; Sutter instruments). The dorsoventral coordinates were marked using the depth of the electrode targeting the PVN (AP= -1 mm ...
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No products found
because this supplier's products are not listed.
Stefano Musardo, et al.,
bioRxiv - Neuroscience 2021
Quote:
... cells were incubated with primary antibody (Oxytocin, 1/10000 dilution, Immunostar #20068 ...
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No products found
because this supplier's products are not listed.
Rafael M. Costa, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... Cells were maintained in Complete Mouse Endothelial Cell Medium (Cell Biologics) containing Endothelial Cell Medium Supplement Kit (Cell Biologics) ...