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No products found
because this supplier's products are not listed.
Guillermo Valenzuela Nieto, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... between the W25 Nanobody and SARS-CoV-2 S1 of Spike RBD was measured by microscale thermophoresis (MST) using a Monolith NT.115PICO instrument (Nanotemper Technologies GmbH). Purified W25 was buffer exchanged into a PBS buffer ...
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No products found
because this supplier's products are not listed.
Chotiwat Seephetdee, et al.,
bioRxiv - Immunology 2022
Quote:
... Blotted proteins were detected with a rabbit polyclonal antibody that recognizes SARS-CoV-2 spike RBD (40592-T62, SinoBiological) and a donkey anti-rabbit IgG-HRP antibody (sc-2077, Santa Cruz Biotechnology).
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No products found
because this supplier's products are not listed.
Lucy G Thorne, et al.,
bioRxiv - Microbiology 2020
Quote:
... Calu-3 or Caco-2 cells were seeded and infected with SARS-CoV-2 in Optical 96-well plates (CellCarrier Ultra, PerkinElmer) and cells were fixed with 4% PFA at the indicated timepoints ...
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No products found
because this supplier's products are not listed.
Vania Passos, et al.,
bioRxiv - Microbiology 2022
Quote:
... SARS-CoV-2-infected and Mock-infected samples were treated with RNA Shield (Zymo Research) to preserve their genetic integrity and inactivate the virus ...
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No products found
because this supplier's products are not listed.
Annalisa Tito, et al.,
bioRxiv - Microbiology 2020
Quote:
... The cells were then infected with SARS-CoV-2 Spike-Pseudotyped Lentivirus (Firefly Luciferase SARS-CoV-2 lentiviral particles-GeneCopoeia) and the control VSV-G protein pseudotyped Lentivirus (HLUC-Lv201 Firefly luciferase − eGFP lentifect-GeneCopoeia ...
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No products found
because this supplier's products are not listed.
B Balakrishnan, K Lai,
bioRxiv - Biochemistry 2021
Quote:
... anti-SARS-CoV-2 Spike Protein was purchased from Origene Technologies Inc ...
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No products found
because this supplier's products are not listed.
Edward Sullivan, et al.,
bioRxiv - Microbiology 2021
Quote:
... as follows: SARS-CoV-2 Spike protein: 1:1000 (Leica, AR9961) 30minutes ...
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No products found
because this supplier's products are not listed.
Brett Stern, Peter Monteleone, Janet Zoldan,
bioRxiv - Bioengineering 2022
Quote:
Viral spike protein (either SARS-CoV-2 Spike Protein, S1 Subunit, RayBiotech, or MERS-CoV-1 Spike Protein, S1 Subunit, BioVision) was added to endothelial culture media of CD34+ iPSC-EP-laden hydrogels at a concentration of 10 μg/mL either one or five days after cell encapsulation ...
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No products found
because this supplier's products are not listed.
Benjamin N. Bell, et al.,
bioRxiv - Immunology 2021
Quote:
His-tagged SARS-CoV-2 RBD (300 nM) was loaded on His1K biosensors (Pall ForteBio) to a load threshold of 0.4-0.6 nm ...
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No products found
because this supplier's products are not listed.
Niayesh Razi, et al.,
bioRxiv - Microbiology 2023
Quote:
The spike-bearing pseudovirus particles (rVSVΔG/SARS-CoV-2-S-D614Gd21-NLucP; Cat No. EGA298-PM, Kerafast, Inc.) were previously described [30] and obtained commercially for this study ...
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No products found
because this supplier's products are not listed.
Romain Rouet, et al.,
bioRxiv - Immunology 2020
Quote:
... we biotinylated SARS-CoV-2 RBD using a terminal AviTag and BirA biotin ligase (Avidity) according to the manufacturer’s protocol ...
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No products found
because this supplier's products are not listed.
Chenjian Gu, et al.,
bioRxiv - Microbiology 2020
Quote:
... pCAGGS-SARS-CoV-2-S that encodes the SARS-CoV-2 Spike gene was generated by GENEWIZ (Suzhou, China). Recombinant adenovirus expressing human ACE2 (Ad5-hACE2 ...
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No products found
because this supplier's products are not listed.
Emanuele Andreano, et al.,
bioRxiv - Immunology 2021
Quote:
... NTD or S2 SARS-CoV-2 protein diluted in carbonate-bicarbonate buffer (E107, Bethyl laboratories) were coated in 384-well plates (microplate clear ...
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No products found
because this supplier's products are not listed.
Hoai Thi Thu Tran, et al.,
bioRxiv - Microbiology 2022
Quote:
SARS-CoV-2 Spike B.1.1.529 (Omicron) variant pseudotyped lentivirus particles were purchased from Biomol (Hamburg, Germany). The transduction assay was performed as described previously [12] ...
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No products found
because this supplier's products are not listed.
Tanay Bhatt, et al.,
bioRxiv - Pathology 2022
Quote:
About 0.1 to 0.2 MOI (Multiplicity of Infection) equivalent of VSV-G and pseudotype SARS-CoV-2 spike virus particles were treated with recombinant LL37 (Tocris) and niacinamide (vitamin B3 ...
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No products found
because this supplier's products are not listed.
Dora Pinto, et al.,
bioRxiv - Immunology 2020
Quote:
... His-tagged RBD of SARS-CoV or SARS-CoV-2 were loaded at 3 µg/ml in KB for 15 minutes onto anti-HIS (HIS2) biosensors (Molecular Devices, ForteBio). Association of mAb and Fab was performed in KB at 15 μg/ml and 5 μg/ml respectively for 5 minutes ...
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No products found
because this supplier's products are not listed.
Bennett J. Davenport, et al.,
bioRxiv - Immunology 2021
Quote:
... and probed with 1 μg/mL of chimeric human anti-SARS-CoV spike antibody (CR3022, Absolute Antibody, Ab01680) in Perm Wash (1X PBS/0.1% saponin/0.1% BSA ...
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No products found
because this supplier's products are not listed.
Kyung Ku Jang, et al.,
bioRxiv - Cell Biology 2022
Quote:
SARS-CoV-2-infected monolayers were fixed with 4% paraformaldehyde (Electron Microscopy Sciences) for 3 hrs at RT ...
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No products found
because this supplier's products are not listed.
Liguo Zhang, et al.,
bioRxiv - Genomics 2020
Quote:
Cells subject to SARS-CoV-2 infection were grown in μ-Slide 8 Well (#1.5 polymer, Ibidi 80826) and fixed with 4% paraformaldehyde/CMF-PBS at room temperature (RT ...
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No products found
because this supplier's products are not listed.
Meenakshi Kar, et al.,
bioRxiv - Immunology 2024
Quote:
... The impact of CD8+ T cell depletions on SARS-CoV-2 infection was also evaluated using anti-CD8β (clone 53-5.8, BioXCell) and isotype mAb IgG2b (clone LTF-2 ...
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No products found
because this supplier's products are not listed.
Bin Zhou, et al.,
bioRxiv - Microbiology 2020
Quote:
... RNA was extracted from Vero E6 cells infected with either recombinant SARS-CoV-2S-614D or SARS-CoV-2S-614G using the NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s guidelines ...
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No products found
because this supplier's products are not listed.
Krishna Gupta, Elisha Krieg,
bioRxiv - Synthetic Biology 2024
Quote:
... COVID_ssRNA & SARS-CoV-2 mimic) triggers were freshly prepared by serial dilution in DNA LoBind tubes (Eppendorf) using nuclease-free water ...
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No products found
because this supplier's products are not listed.
Ferdos Abid Ali, et al.,
bioRxiv - Cell Biology 2023
Quote:
Sf9 insect cells (VWR, #EM71104-3) were maintained in ExCell 420 serum-free media (Sigma ...
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SARS-CoV-2 Spike Glycoprotein is a recombinant protein expressed in Mammalian cells.
Cat# abx620076-50UG,
50 µg USD $333.5
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Bálint Kiss, et al.,
bioRxiv - Biophysics 2020
Quote:
... 100 μl of 10 μg/ml SARS-CoV-2 Spike Glycoprotein Antibody (#abx376478, Abbexa Ltd, Cambridge, UK) was then added ...
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SARS-CoV-2, Recombinant Protein, Eta, variant B.1.525, Spike S1+S2 Protein, Q52R, A67V, HV69-70...
Cat# VReP-GCY091,
1.0 case, Inquiry
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Xiaotian Tan, et al.,
bioRxiv - Bioengineering 2020
Quote:
... The human-cell-expressed SARS-CoV Spike S1-His recombinant protein was purchased from Creative Biolabs (VAng-Wyb7337). The recombinant CR3022 therapeutic antibody was purchased from Creative Biolabs (MRO-1214LC) ...
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No products found
because this supplier's products are not listed.
Kevin W. Ng, et al.,
bioRxiv - Immunology 2021
Quote:
... mice were injected intraperitoneally with 25 µg recombinant SARS-CoV-2 S2 (CV2006; LifeSensors) in monophosphoryl lipid A adjuvant (Sigma Adjuvant System ...
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No products found
because this supplier's products are not listed.
Christopher J. Emig, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... SARS-CoV-2 Delta Variant pseudovirus (eEnzyme) was diluted 1:2 in DMEM complete media to a pseudoviral particle concentration of 5e7/ml ...
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No products found
because this supplier's products are not listed.
Mariana F. Tioni, et al.,
bioRxiv - Immunology 2021
Quote:
Viruses and control recombinant SARS-CoV-2 spike protein (LakePharma) were denatured with Laemmli sample buffer (Alfa Aesar) by heating at 95 °C for 10 minutes ...
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No products found
because this supplier's products are not listed.
Wael Bahnan, et al.,
bioRxiv - Immunology 2021
Quote:
... making them permissive to SARS-CoV-2 infection (Jackson laboratories). One day after infection ...
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No products found
because this supplier's products are not listed.
Gabriela Mattoso Coelho, et al.,
bioRxiv - Microbiology 2023
Quote:
... washed three times in PBS with 0.05% tween 20 and incubated with a commercial anti-SARS-CoV-2 Spike antibody (1/1000) (MP Biomedicals, Irvine, CA, USA) or the in-house anti-SARS-CoV-2 mAb (1/400) ...
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No products found
because this supplier's products are not listed.
Mehdi Moustaqil, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... coli C41(DE3) cells were transformed with the SARS-CoV-2 pET-28a-nsp5 plasmid (IDT DNA). Single colonies were used to inoculate LB media supplemented with kanamycin (50 μg/mL) ...
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No products found
because this supplier's products are not listed.
James M. Burke, et al.,
bioRxiv - Immunology 2021
Quote:
... and SARS-CoV-2 smFISH probes were designed using Stellaris smFISH probe designer (Biosearch Technologies) available online at http://www.biosearchtech.com/stellaris-designer ...
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No products found
because this supplier's products are not listed.
Alexandria N. Miller, et al.,
bioRxiv - Biophysics 2022
Quote:
... purified SARS-CoV-2 Orf3a was reconstituted in soybean polar lipid extract (Avanti Polar Lipids) at 1:10 and 1:100 wt:wt protein to lipid ratios ...
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No products found
because this supplier's products are not listed.
Claire T. Deakin, et al.,
bioRxiv - Immunology 2021
Quote:
Full-length SARS-CoV-2 and HCoV-OC43 nucleoproteins were produced by expression in Escherichia coli C43(DE3) cells (Lucigen) and purified as previously described (12) ...
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No products found
because this supplier's products are not listed.
Hayden T. Pacl, et al.,
bioRxiv - Microbiology 2021
Quote:
... was treated with 300 nM of purified recombinant SARS-Cov2 NSP13 with an N-terminal His-tag (Cayman Chemicals #30589) in the presence of 50 mM Tris pH-7.4 ...
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No products found
because this supplier's products are not listed.
Galen J. Correy, et al.,
bioRxiv - Biochemistry 2022
Quote:
The gene encoding SARS-CoV-2 2-170 NSP3 Mac1 was cloned into a pET-11a plasmid (Bio Basic) and transformed into E ...
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No products found
because this supplier's products are not listed.
Mohamed Fareh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 RNA was converted to cDNA using the SensiFAST cDNA kit (#BIO-65053, BioLine) with 10 μL of RNA extract per reaction following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Huan Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... anti-His tag (Solarbio Life Sciences, China, K200060M), anti-toll-like receptor-4 (TLR-4 ...
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No products found
because this supplier's products are not listed.
Anna C. Fagre, et al.,
bioRxiv - Microbiology 2020
Quote:
... Horizontal whole-lung slides of consecutive planes of SARS-CoV-2-infected and antibody-treated lungs were digitalized by Olympus histoslide scanner to create image data files that can be used for further digital image analysis (DIA) ...
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No products found
because this supplier's products are not listed.
Bert Schepens, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... spiked controls and diluted samples were mixed with 3 nM VHH72-h1 (S56A)-Flag3-His3 and 2.5 nM biotinylated SARS-CoV-2 RBD protein in white low binding 384-well microtitre plates (F-bottom, Greiner Cat nr 781904). After an incubation for 1 hour at room temperature ...
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No products found
because this supplier's products are not listed.
Ge Song, et al.,
bioRxiv - Immunology 2020
Quote:
... Proteins without His tags were purified with GNL columns (Vector Labs). The bound proteins were washed with PBS and then eluted with 50 mL of 1M Methyl α-D-mannopyranoside (Sigma M6882-500G ...
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No products found
because this supplier's products are not listed.
Biao Zhou, et al.,
bioRxiv - Microbiology 2021
Quote:
... immunofluorescence staining was performed on deparaffinized and rehydrated tissue sections using a rabbit anti-SARS-CoV-2-NP protein antibody together with an AF488-conjugated anti-rabbit IgG (Jackson ImmunoResearch, PA, USA). Briefly ...
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No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The levels of S protein in different brain regions upon its intranasal infusion were measured using His-Tag protein ELISA kit that allows detection and quantification of poly-histidine-tagged proteins in a biological sample (Cell Biolabs Inc., CA, USA). The experimental procedure was performed as recommended by the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Michele F. Buono, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Cells were lysed and sonicated with the S2 sonicator (Covaris) to shear the chromatin into 100-300 bp fragments following the program stated in the protocol ...
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No products found
because this supplier's products are not listed.
Punn Augsornworawat, et al.,
bioRxiv - Bioengineering 2019
Quote:
Stage 2: 3 days in S2 basal media with 50 ng/ml KGF (Peprotech; AF-100-19).
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No products found
because this supplier's products are not listed.
K.M. Martin, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Microscopy of insect cells was performed at 72 hours post-transfection using an Eclipse Ts2R (Nikon) epifluorescent microscope using an S Plan Fluor ELWD 40x/.060 objective with a CFI 10x/22 eyepiece ...
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No products found
because this supplier's products are not listed.
David Ollitrault, et al.,
bioRxiv - Cell Biology 2021
Quote:
... cells were placed for 2 days in Endothelial Cell Growth Medium 2 (EGM-2; PromoCell) supplemented with 0.5 ng/ml of vascular endothelial growth factor 165 (VEGF) ...
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No products found
because this supplier's products are not listed.
Yi Xie, J. Todd Blankenship,
bioRxiv - Cell Biology 2020
Quote:
... after genome extraction from larva or adults (E.Z.N.A insect DNA kit, Omega Biotek).
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No products found
because this supplier's products are not listed.
Donghwi Bae, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 2% SM1 (Stem Cell Technologies), 15mM HEPES (Gibco) ...
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No products found
because this supplier's products are not listed.
Aram Avila-Herrera, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Human Anti-SARS-CoV-2 Spike-RBD Clone S309 mAb and Human Anti-SARS-CoV-2 Spike-RBD REGN10987 mAb (Imdevimab) were purchased from Cell Sciences (Newburyport MA, USA). 2130-1-0114-112 (LLNL-112 ...