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Citations for Eppendorf :
1 - 50 of 753 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... COVID_ssRNA & SARS-CoV-2 mimic) triggers were freshly prepared by serial dilution in DNA LoBind tubes (Eppendorf) using nuclease-free water ...
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bioRxiv - Microbiology 2020Quote: ... mouth rinse-treated SARS-CoV-2 viruses were concentrated by centrifugation at 14,000 rpm in a centrifuge (Eppendorf) at 4°C for 2 h as described previously (Holmes et al ...
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bioRxiv - Immunology 2021Quote: ... 293T-hACE2 cells were transfected with 0.5 μg of SARS-CoV-2-RBD plasmid (a gift from Dr. Abraham Pinter) in each well in a 24-well plate (Eppendorf) for 48 hours at 37°C under 5% (v/v ...
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bioRxiv - Immunology 2020Quote: ... 293T-hACE2 cells were transfected with 0.5 μg of SARS-CoV-2-RBD plasmid (a gift from Dr. Abraham Pinter) each well in a 24-well plate (Eppendorf) for 48 hours at 37°C under 5% (v/v ...
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bioRxiv - Immunology 2020Quote: ... 293T-hACE2-FFLuc-GFP-RBD cells were transfected with 0.25 μg of SARS-CoV-2-RBD plasmid and 0.25 μg of pHAGE-FFLuc-GFP each well in a 24-well plate (Eppendorf) for 48 hours at 37°C under 5% (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... 1 mL of 200 µM monomeric αSyn was incubated in fibrillation buffer (Supplementary Table S2) in a 1.5 mL Protein LoBind Eppendorf tube (Eppendorf, Germany) on an orbital shaker (PCMT Thermoshaker Grant-bio ...
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bioRxiv - Microbiology 2022Quote: ... the cells were transferred to 2 mL tubes (cat: 0030120094, Eppendorf) in 1.5 mL aliquots for Unstained Control cells ...
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bioRxiv - Cancer Biology 2023Quote: SK-N-BE(2) cells (6000-8000 cells/well) were seeded in 24-well plates (Eppendorf) overnight and prior to treatment with retinoic acid (RA)(10 μM ...
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bioRxiv - Neuroscience 2020Quote: ... Individual brown planthoppers were then injected with 40 nl of peptide solution (2.25 pmol/insect) or 40 nl of PBS in the lateral side of the abdomen using a FemtoJet system (Eppendorf-Nethler-Hinz, Hamburg, Germany). Immediately after injection ...
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bioRxiv - Biophysics 2023Quote: ... The blood was centrifuged at 250 RCF (relative centrifugal force) and 7 rad/s2 acceleration for 20 min (5810 R, Eppendorf, Hamburg, Germany). The platelet rich plasma (PRP ...
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bioRxiv - Genomics 2020Quote: ... The cell-free supernatant was carefully pipetted into 2 mL LoBind tubes (Eppendorf LoBind microcentrifuge tubes ...
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bioRxiv - Biophysics 2021Quote: ... tfb6Δ strain containing TAP tags on TFIIH subunits Tfb4 and Ssl2 was grown by fermenter (Eppendorf) in 100 L of YPAD medium to OD 10.0 ...
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bioRxiv - Systems Biology 2021Quote: ... Next the spheroplasted cells were centrifuged at 1500 rpm for 2 min (Eppendorf centrifuge), the supernatant was removed and the cells were resuspended in 400 µL of breaking buffer which contained 10 mM Tris ...
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bioRxiv - Genomics 2021Quote: ... 2 mL of cells were aliquoted to a 15 mL conical and centrifuged (Eppendorf 5424R) at 500 g for 5 min ...
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bioRxiv - Microbiology 2022Quote: Cells (2 ml cultures) were spun down (30 s Eppendorf centrifuge, 14,000 rpm, 4 °C), resuspended in 0.4 ml ice-cold growth medium and added to a screw cap Eppendorf tube containing 1.5 g glass beads (0.1 mm) ...
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... Cells (2 OD600) were harvested at 600 x g for 1 min (MiniSpin Centrifuge, Eppendorf) and the supernatants were discarded ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 million cells were added to a 5 mL DNA LoBind tube (Eppendorf, cat. no. 0030108310), centrifuged at 400 x g for 4 min ...
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bioRxiv - Biophysics 2019Quote: ... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
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bioRxiv - Physiology 2023Quote: ... for EVs isolation on cell culture flask T-75 (1.5 − 2 × 106/ per flask; Eppendorf, Hamburg, Germany) and for immunostaining on Cell Imaging Cover Glasses (0.1 × 106 / per chamber ...
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bioRxiv - Cell Biology 2022Quote: For esiRNA transfection 35,000 U2OS cells were seeded in 2 ml medium in 6-well plates (Eppendorf) the evening before transfection ...
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bioRxiv - Cell Biology 2022Quote: U-2 OS cells were grown in 24-well glass bottom plates (170 µm coverglass bottom; Eppendorf #0030741021) coated with poly-l-lysine and treated with 200 µM oleate-BSA complex for 24 hr ...
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bioRxiv - Microbiology 2022Quote: ... 0.5 mL subsamples of cell cultures normalized as above were dispensed into 2 mL tubes (cat: 0030120094, Eppendorf) and stained with MitoTracker Orange CMTMRos (cat ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... pH 7.6) and injected into 1 to 2-cell-stage embryos by using a microinjector FemtoJet 5247 (Eppendorf). The sequences and the concentrations of MOs (Gene Tools ...
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bioRxiv - Cell Biology 2020Quote: ... and injected into the nucleus of HeLa or U-2 OS cells using an Eppendorf FemtoJet® Microinjector (Eppendorf). After media were changed ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were sorted using a 100 um nozzle (∼20 PSI) into 2 ml protein LoBind Eppendorf tubes (Eppendorf 0030108132) placed in a cooling holder ...
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bioRxiv - Systems Biology 2021Quote: ... Cell lysates were transferred to a new tube and were centrifuged for 2 min at full speed (Eppendorf centrifuge) and the supernatant was carefully mixed with 1 volume of 70% HPLC-grade ethanol ...
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bioRxiv - Developmental Biology 2020Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using FemtoJet or FemtoJet 4i (Eppendorf; 5252000013) micro-injectors ...
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bioRxiv - Neuroscience 2022Quote: ... cell pellets were resuspended in 1mL of ice-cold PBS and transferred to 2 mL LoBind Eppendorf tubes (Eppendorf #022431102). Cells were centrifuged for 2 minutes at 800rpm before being flash-frozen on dry ice ...
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bioRxiv - Molecular Biology 2020Quote: The cell cultures were then transferred into 2 ml reaction tubes and placed in slots in a table-top thermomixer (Eppendorf) set to 37° C and shaking at 700 rpm for five minutes prior to pulse-labelling ...
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bioRxiv - Microbiology 2022Quote: ... The cell suspension was then transferred into 2 mL tubes and centrifuged at 16 000g and 4°C (Eppendorf, Germany) to pellet the cells ...
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bioRxiv - Developmental Biology 2024Quote: ... One nL morpholino (dissolved in 0.2 M KCl with 0.05% phenol red at 3 mg/mL) was microinjected into haploid embryos at the 1 or 2-cell stage using FemtoJet and InjectMan NI2 (Eppendorf).
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bioRxiv - Evolutionary Biology 2023Quote: ... and InjectMan NI 2 (Eppendorf). Cas9 protein (500 ng/μL) ...
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bioRxiv - Neuroscience 2023Quote: ... The plasmid injection solution was injected into Hydra vulgaris AEP 1-cell stage embryos using an Eppendorf FemtoJet 4x and Eppendorf InjectMan NI 2 microinjector (Eppendorf; Hamburg, Germany) under a Leica M165 C stereo microscope (Leica Microscopes ...
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bioRxiv - Bioengineering 2024Quote: ... Samples were diluted to 2 x 107 cells per mL and 50 µL of each sample was added to 5 mL tubes (Eppendorf, Hamburg, DE), to constitute 1 million cells per flow sample.
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bioRxiv - Molecular Biology 2019Quote: ... using a Realplex 2 thermocycler (Eppendorf). The PCR conditions were 95°C for 3 min ...
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bioRxiv - Immunology 2022Quote: ... The micromanipulator (Eppendorf InjectMan NI 2) was mounted on an Andor Spinning Disc Microscope and microinjection was performed using the microinjector FemtoJet with a 100X oil immersion objective to facilitate immediate visualization and image acquisition after microinjection ...
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bioRxiv - Cell Biology 2023Quote: ... The micromanipulator (Eppendorf InjectMan NI 2) was positioned and microinjection was performed using microinjector (Eppendorf FemtoJet ...
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bioRxiv - Microbiology 2024Quote: ... and Mastercycler ep Realplex 2 (Eppendorf). Data was normalized by GAPDH or sigA expression level and all primers were designed using GenScript primer design software ...
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bioRxiv - Cancer Biology 2024Quote: ... on a RealPlex 2 Thermocycler (Eppendorf).
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bioRxiv - Developmental Biology 2024Quote: ... One- or 2-cell stage embryos were injected with approximately 20 nl of the injection mixture (manually or using an Eppendorf FemtoJet 4x microinjector) and maintained at 20°C until the 64-cell stage ...
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bioRxiv - Cancer Biology 2023Quote: ... using a RealPlex 2 thermocycler (Eppendorf, 2894). All primers were designed and purchased from Integrated DNA Technologies and their sequences are listed in Supplementary file 1.
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bioRxiv - Developmental Biology 2021Quote: ... or double (for qRT-PCR) blastomere microinjections of 2-cell (E1.5) stage embryos were performed using the FemtoJet 4i (Eppendorf, Hamburg, Germany; Cat. No. 5252000013) micro-injector ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were spun down (5 min, 800 g•, 2 °C, minimal acceleration and break, Eppendorf 5810 R with swing-×bucket rotor A-4-44). The medium was discarded and the cells were dissociated in 15 mL of ice cold ACK solution (0.15 M NH4Cl ...
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bioRxiv - Cancer Biology 2021Quote: ... 30-70 kDa lane portions were excised into 2×2 mm cubes and transferred to Protein Lo-Bind tubes (Eppendorf). Excised gels were partitioned into tubes ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were fixed in 2% paraformaldehyde for 55 minutes at room temperature in 2 mL Protein LoBind tubes (Eppendorf 022431064). Fixative was removed and tissues were washed 4x 10 minutes with 1.5 mL PBS with 0.5% Triton X-100 (PBT) ...
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bioRxiv - Biochemistry 2019Quote: ... in 2 mL Protein Lo Bind Tubes (Eppendorf) using the following protocol ...
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bioRxiv - Cell Biology 2021Quote: ... in 2 mL Protein Lo Bind Tubes (Eppendorf) using the following protocol ...
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bioRxiv - Immunology 2021Quote: ... and collected into sterile 2 ml tubes (Eppendorf). All samples were immediately snap frozen in dry ice and stored at –80 °C until DNA extraction.
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bioRxiv - Biochemistry 2023Quote: ... in 2 mL Protein Lo Bind Tubes (Eppendorf) using the following protocol ...
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bioRxiv - Plant Biology 2023Quote: ... in individual 2 mL safe-lock tubes (Eppendorf). The suspensions were briefly vortexed to homogeneity and incubated at room temperature for 2 hours ...