-
No products found
because this supplier's products are not listed.
Tianyi Zhang, et al.,
bioRxiv - Immunology 2023
Quote:
... day 0 and day 21) with 5 μg/dose of recombinant SARS-CoV-2 Spike protein (S1+S2 extracellular domain) (Bon Opus Biosciences, #BP040) adjuvanted with 100 μg/dose alhydrogel adjuvant 2% (InvivoGen ...
-
No products found
because this supplier's products are not listed.
Chengjin Ye, et al.,
bioRxiv - Microbiology 2022
Quote:
... and SARS-CoV-2 M protein was purchased from ProteoGenix (Catalog# PX-COV-P025).
-
No products found
because this supplier's products are not listed.
Anne-Catherine Fluckiger, et al.,
bioRxiv - Immunology 2021
Quote:
The expression of SARS-CoV-2 S protein in eVLP preparations was analyzed by western blotting as described previously [18] using rabbit polyclonal Ab (pAb) anti-spike protein of SARS-CoV-2 (Immune Technology Corp) followed by detection with goat anti-rabbit IgG-Fc horseradish peroxydase-conjugated (Bethyl) ...
-
No products found
because this supplier's products are not listed.
Carmine Varricchio, et al.,
bioRxiv - Microbiology 2022
Quote:
RNA was extracted from isolated clinical SARS-CoV-2 with E.Z.N.A total RNA (Omega Bio-Tek). Maxima H Minus cDNA Synthesis (Thermofisher ...
-
No products found
because this supplier's products are not listed.
Meenakshi Kar, et al.,
bioRxiv - Immunology 2024
Quote:
SARS-CoV-2 RNA was isolated using RNAzol® RT Column Kit (Molecular Research Center, Inc.) as per manufacturer’s instructions from the B.1.351 stock samples (n=4 ...
-
No products found
because this supplier's products are not listed.
Beibei Wu, et al.,
bioRxiv - Microbiology 2021
Quote:
ELISA was performed on cell culture supernatants from indicated control or SARS-CoV-2-infected cells for detection of IFN-β (PBL Assay Science, #41410) or IL-6 (ThermoFisher Scientific ...
-
No products found
because this supplier's products are not listed.
Guoli Shi, et al.,
bioRxiv - Immunology 2020
Quote:
... All transient transfections for SARS-CoV-2 infection experiments were performed using LipoJet transfection reagent (Signagen Laboratories). siRNAs targeting IFITM1 (s16192) ...
-
No products found
because this supplier's products are not listed.
Mesfin Meshesha, et al.,
bioRxiv - Bioengineering 2023
Quote:
... and SARS-CoV-2 lineage B.1.1.529 (Omicron Variant) culture fluid (UV inactivated, 0810642UV, Zeptometrix LLC, USA) and (heat inactivated ...
-
No products found
because this supplier's products are not listed.
Raphael J. Eberle, et al.,
bioRxiv - Biochemistry 2021
Quote:
SARS-CoV-2 3CLpro activity assay was performed using a fluorogenic substrate DABCYL- KTSAVLQ↓SGFRKME-EDANS (Bachem, Switzerland) in a buffer containing 20 mM Tris pH 7.2 ...
-
No products found
because this supplier's products are not listed.
J. Rafael Ciges-Tomas, et al.,
bioRxiv - Biophysics 2022
Quote:
The synthetic gene for the SARS-CoV-2 NCTD (residues 256 to 364) cloned into the vector pET28a(+) was provided by Biomatik. The insert is in frame with the N-terminal His6-tag ...
-
No products found
because this supplier's products are not listed.
Nicholas You Zhi Cheang, et al.,
bioRxiv - Immunology 2023
Quote:
... were stimulated in a 96-well round-bottom plate with 5 µg/mL ancestral and variant SARS-CoV-2 RBD peptides and 1 µg/mL anti-CD28 (40-0281-M001; Tonbo Biosciences, San Diego, California, USA) at 37°C and 5% CO2 ...
-
No products found
because this supplier's products are not listed.
Reshmi Mukherjee, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Synthetic 2′-FL (s2′-FL) was purchased from Carbosynth (www.carbosynth.com). The c2′-FL contains 94.1 % 2′-FL ...
-
No products found
because this supplier's products are not listed.
Dominik Saul, et al.,
bioRxiv - Physiology 2021
Quote:
... An Insect Pin (Fine Science Tools, 26001-30 ...
-
No products found
because this supplier's products are not listed.
Ashok Pabbathi, et al.,
bioRxiv - Biophysics 2021
Quote:
... We incubated the washed beads with 0.25 mg/mL mouse monoclonal biotinylated anti-His Tag monoclonal antibody (AS-61250-BIOT, Anaspec Inc., Fremont, CA) for 2 hours at 22°C ...
-
No products found
because this supplier's products are not listed.
Valentina Fajner, et al.,
bioRxiv - Animal Behavior and Cognition 2020
Quote:
... fly ovaries were dissected in Drosophila S2 medium with 10 % of FBS (Euroclone) and incubated with 2.5% 1,6-hexanediol on an orbital shaker at room temperature for 5 minutes (Fig ...
-
No products found
because this supplier's products are not listed.
Marissa I. Nufer, et al.,
bioRxiv - Genetics 2024
Quote:
... Two 3 mm stainless steel beads were added to the tubes containing insect legs and homogenized using a bead beater (Biospec Products, Bartlesville, OK) for 1 min at the highest speed ...
-
No products found
because this supplier's products are not listed.
Piotr T. Wysocki, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... and MCDB-105 (Cell Applications, San Diego, CA, USA; ratio 2:1:1). Culture media were supplemented with 10% fetal bovine serum (FBS ...
-
No products found
because this supplier's products are not listed.
Camilla Margaroli, et al.,
bioRxiv - Pathology 2022
Quote:
... Anti-SARS-CoV-2 was conjugated to PE / R-Phycoerythrin (Expedeon Lightning-Link R-PE Conjugation Kit / Abcam ...
-
No products found
because this supplier's products are not listed.
Daniela Bottero, et al.,
bioRxiv - Immunology 2023
Quote:
... LightMix® Modular SARS-CoV (COVID19) RdRp (TIB MOLBIOL), and RNAsa P following the protocol developed by Dr ...
-
No products found
because this supplier's products are not listed.
Fred D. Mast, et al.,
bioRxiv - Biochemistry 2021
Quote:
... with triplicate assays of harvested RNA from each SARS-CoV-2-infected AEC donor cell line (Genesig® Coronavirus Strain 2019-nCoV Advanced PCR Kit, Primerdesign®, Southampton, UK). The concentration of RNA harvested from AECs was used to normalize the qPCR data and was measured on a spectrophotometer (Nanodrop®).
-
No products found
because this supplier's products are not listed.
Ye Qiu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Mouse anti-His tag monoclonal antibody (Bioworld, USA, 1:5000) was used as the first antibody ...
-
WB,IF,IP,IHC,FC,ELISA
Cat# A5712, SKU# A5712-20ul,
20ul, $47.00
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Xuxiao He, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... and Nickel Magnetic Beads for His tag protein purification (Bimake, B23602) according to the manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Christine Salaun, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Insect cells Sf9 (600100, Oxford Expression Technologies, UK) and Sf9 TriEx (71023-3 ...
-
No products found
because this supplier's products are not listed.
Nancy G. Azizian, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... 5 μl of 2 mM biotin-alkyne-tag (Click Chemistry Tools, 1266), and 5 μl of 200 mM CuSO4 were added in the specified order to 5 ml of PBS (pH 7.8) ...
-
No products found
because this supplier's products are not listed.
Anthony C. Fredericks, et al.,
bioRxiv - Microbiology 2019
Quote:
... at the time of cell seeding using TransIT-insect transfection reagent (Cambridge Biosciences, Cambridge, UK) as per manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Ferdinand Roesch, et al.,
bioRxiv - Microbiology 2022
Quote:
... Aag2 were maintained in Schneider’s insect medium (Genesee Scientific) with 7% FBS ...
-
No products found
because this supplier's products are not listed.
Jillian M. DiMuzio, et al.,
bioRxiv - Immunology 2021
Quote:
Spike-expressing pseudovirus was generated with System Bioscience’s pPACK-SPIKE packaging system (System Biosciences, Cat # CVD19-50A-1) as per manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Jimena Leyria, Ian Orchard, Angela B. Lange,
bioRxiv - Molecular Biology 2020
Quote:
... female insects were fed on defibrinated rabbit blood (Cedarlane, Burlington, CA) to initiate egg growth ...
-
No products found
because this supplier's products are not listed.
Dennis Botman, et al.,
bioRxiv - Microbiology 2023
Quote:
... Cells were grown in either a CLARIOstar (all growth assays except for Fig. S2) or Fluostar plate reader (for Fig. S2, BMG LABTECH, Ortenberg, Germany) at 30°C and 700 rpm orbital shaking and OD600 was measured every 5 minutes.
-
No products found
because this supplier's products are not listed.
Nanami Sakata, et al.,
bioRxiv - Plant Biology 2022
Quote:
... L-His (Tokyo Chemical Industry), and L-Lys (Nacalai tesque ...
-
No products found
because this supplier's products are not listed.
Ana Zúñiga, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... For experimental measurements the cells were grown in Azure Hi-Def medium (Teknova, 3H5000) supplemented with 0.4% of glycerol ...
-
No products found
because this supplier's products are not listed.
Yunye Zhu, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Transformants scraped from densely plated transformation plates were inoculated into fresh SC-His medium with 2% raffinose (Amresco, J392) at 0.25 x 107 cells/ml and grown until 0.5-0.8 x 107 cells/ml ...
-
No products found
because this supplier's products are not listed.
Brian M. Debo, Ben Mallory, Andrew B. Stergachis,
bioRxiv - Genomics 2022
Quote:
The absolute quantity of DNA-m6A in S2 cell nuclei treated with 20 U of Hia5 for 10 min was determined using Pacific Biosciences (PacBio) Circular consensus sequencing ...
-
No products found
because this supplier's products are not listed.
Yanzhou Zhang, et al.,
bioRxiv - Cell Biology 2021
Quote:
His-tagged or His-sumo-tagged protein was purified using NiNTA beads (Anatrace, Cat. No. SUPER-NINTA25). Briefly ...
-
No products found
because this supplier's products are not listed.
Viviane Kremling, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... baculovirus gp64 signal sequence under control of the very late polyhedrin promoter was inserted into the insect cell vector plasmid pACEBac1 (Addgene, LGC Standards Teddington, UK) by using another synthetic gene (VK18 ...
-
No products found
because this supplier's products are not listed.
Nathan H. Murray, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Three 10 cm plates were seeded with 1.4×106 cells for each cell line in IMDM (Thermo 12440053) supplemented with 10% heat inactivated FBS (HI FBS)(Atlanta Biologicals) and 100 U/mL Penicillin-Streptomycin (Pen/Strep)(Thermo 15140122) ...
-
CSC 2F0 cells were isolated by centrifugal counterflow elutriation from 250 individual donor...
Cat# CSC 2F0 V,
1.0 Units, $405.0
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Lorna Ewart, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... 2% Culture-Boost (Cell Systems), and 10% Fetal Bovine Serum (FBS ...
-
No products found
because this supplier's products are not listed.
Sarah J. Jefferson, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The mouse was briefly anesthetized with isofluorane and the magnetic ear tag was placed on a mouse’s ear using the ear tag applicator (#56791 Stoelting). Each mouse received one ear tag because signal was sufficiently strong with one tag and ears tended to get stuck together when both received ear tags ...
-
No products found
because this supplier's products are not listed.
Nadiya Khyzha, Steven Henikoff, Kami Ahmad,
bioRxiv - Genomics 2022
Quote:
... S2 nuclei were isolated and bound to paramagnetic Concanavalin A (ConA) beads (Bangs Laboratories). To do so ...
-
No products found
because this supplier's products are not listed.
Andrew J. Stout, et al.,
bioRxiv - Bioengineering 2022
Quote:
... or Beefy-R (Hi-Def B8 supplemented with 0.4 mg/mL RPI). Beefy-9 and Beefy-R were prepared immediately before use ...
-
No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
-
No products found
because this supplier's products are not listed.
Ben S. Ou, et al.,
bioRxiv - Bioengineering 2023
Quote:
... HIS Lite Cy3 Bis NTA-Ni Complex was purchased from AAT Bioquest. Unless otherwise stated ...
-
No products found
because this supplier's products are not listed.
Ranjan Ramachandra, et al.,
bioRxiv - Systems Biology 2020
Quote:
... cells were fixed with 2% glutaraldehyde (18426, Ted Pella Incorporated) in 0.1M sodium cacodylate buffer ...
-
No products found
because this supplier's products are not listed.
Binayak Sarkar, et al.,
bioRxiv - Microbiology 2024
Quote:
... while DAGs/TAGs were resolved using a Luna 5U C-5 column (Phenomenex) using established solvent systems.
-
No products found
because this supplier's products are not listed.
Sara Y. Brucker, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... and the desired amount of cell suspension mixed with Basement Membrane Extract (Type 2, 3533-001-2, Trevigen; BME) at a ratio of 65% BME to 35% cell suspension ...
-
No products found
because this supplier's products are not listed.
Nora Kory, et al.,
bioRxiv - Cell Biology 2020
Quote:
... cells were incubated in RPMI containing 2 mM 13C5,15N2-glutamine (Cambridge Isotope Labs) as the sole glutamine source for 2 hours before metabolites were extracted and quantified as described above with the exception that no internal standards were added to the extraction buffer ...
-
No products found
because this supplier's products are not listed.
Simon P. Fraessle, et al.,
bioRxiv - Immunology 2022
Quote:
... 2×104 Target cells were seeded into 96 well E-Plate (ACEA Biosciences Inc.) and rested overnight ...
-
No products found
because this supplier's products are not listed.
Inga Kristaponyte, et al.,
bioRxiv - Neuroscience 2020
Quote:
Single-unit extracellular spikes were recorded with micropipettes which were pulled from quartz glass tubes (Sutter Instrument, Novato, CA; product QF100-50-10) using a laser electrode puller (Sutter Instrument ...
-
No products found
because this supplier's products are not listed.
Brian Kang, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... 6e6 cells were then electroporated in a 2 mm path length electroporation cuvette (BTX Harvard Apparatus; Holliston, MA) with 5μg (HCV ...
-
No products found
because this supplier's products are not listed.
Laura Maria Florez, et al.,
bioRxiv - Immunology 2023
Quote:
... The muMECs were seeded in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min (from Cell Biologics, Euromedex) and cultures were divided twice a week or as necessary ...