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Citations for KeraFAST :
1 - 50 of 142 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: The spike-bearing pseudovirus particles (rVSVΔG/SARS-CoV-2-S-D614Gd21-NLucP; Cat No. EGA298-PM, Kerafast, Inc.) were previously described [30] and obtained commercially for this study ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 pseudovirus (prepared by Nexelis, using Kerafast system ...
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bioRxiv - Microbiology 2020Quote: ... transfected with SARS-CoV-2 S protein were inoculated with VSV-G pseudotyped ΔG-luciferase VSV (Kerafast, MA). After a 2 hour incubation at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... and glycoprotein (G) proteins (courtesy of Dr. Michael Whitt; KeraFAST) (53) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 293T/Spike cells were incubated with pseudotyped G*ΔG-GFP rVSV (Kerafast EH1024-PM) at MOI ∼3 for 1 h ...
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bioRxiv - Immunology 2023Quote: ... and the pcDNA3.1-spike protein plasmids and G*ΔG-VSV (Kerafast) with firefly luciferase in place of VSV-G were transfected into 293T cells (American Type Culture Collection [ATCC] ...
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bioRxiv - Neuroscience 2021Quote: ... α AP tag (1 µg/mL; Kerafast, EGO016). After three washes in a buffer containing (in mM ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were infected at a multiplicity of infection of 3-5 for 2 hours at 37°C with rVSV-ΔG pseudovirus complemented in trans with the G glycoprotein and bearing a luciferase gene (Kerafast). Cells were then washed 6 times with DMEM + 10% FBS and returned to culture ...
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bioRxiv - Microbiology 2023Quote: ... a BlueScript backbone plasmid under T7 polymerase promoter control encoding VSV Indiana backbone in which the VSV glycoprotein (G) gene has been replaced with GFP (pVSV-ΔG-GFP) was purchased from Kerafast. The pVSV-ΔG-GFP was modified by cloning the codon optimized ORF of RABV G protein of Egyptian strain (GenBank accession number MK760770.1 ...
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bioRxiv - Microbiology 2022Quote: ... BHK21/WI-2 cells (Kerafast #EH1011) were maintained in DMEM (Thermo Fisher #11965092 ...
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bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells (Kerafast) were transfected with the S-expression plasmid and subsequently infected with VSVΔG-firefly-luciferase as previously described.6 For the neutralization assay ...
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bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells (Kerafast) were transfected with the S expression plasmid and subsequently infected with VSV△G-firefly-luciferase as previously described [40] ...
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bioRxiv - Immunology 2021Quote: ... BHK21/WI-2 cells (Kerafast, EH1011) were transfected with the Wuhan-1 strain (Genbank # ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21/WI-2 cells (Kerafast, MA) were maintained in DMEM (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... BHK-21/WI-2 cells (Kerafast, MA) transfected with SARS-CoV-2 S protein were inoculated with VSV-G pseudotyped ΔG-luciferase VSV (Kerafast ...
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bioRxiv - Immunology 2021Quote: ... BHK-21/WI-2 cells (Kerafast, EH1011) were transfected with the spike expression plasmid and subsequently infected with VSVΔG-firefly-luciferase as previously described (Whitt ...
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bioRxiv - Immunology 2021Quote: Baby Hamster kidney BHK-21/WI-2 cell line (Kerafast, EH1011), African Green monkey kidney-derived Vero E6 cell line (ATCC ...
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bioRxiv - Immunology 2022Quote: SARS-CoV-2 Spike pseudotyped ΔG-VSV-luciferase was generated by nucleofection of BHK cells (maintained in DMEM/F12 with 10%FBS and 5%TPB) with Spike-expressing plasmid followed by transduction with G-pseudotyped ΔG-luciferase (G*ΔG-luciferase) rVSV (Kerafast) 18-24 hours later ...
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bioRxiv - Microbiology 2021Quote: ... BHK-21/WI-2 cells were purchased from Kerafast (Boston, MA, USA) and cultured in D5+ media ...
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bioRxiv - Immunology 2023Quote: ... CRL-3216) was transfected with the spike protein-expressing plasmids and then infected with G*ΔG-VSV virus (Kerafast, EH1020-PM). After culturing ...
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bioRxiv - Microbiology 2022Quote: Baby hamster kidney cells (BHK-21/WI-2, Kerafast # EH1011, Boston, MA, USA) and African Green Monkey Cell Line (VeroE6 ...
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bioRxiv - Immunology 2022Quote: VSV pseudotyped with S of SARS-CoV-2 were generated in BHK-21/WI-2 cells using a the ΔG-GFP (G*ΔG-GFP) rVSV kit (Kerafast, Boston, MA, USA) and S sequences cloned into an expression plasmid under the control of the CMV promoter as described previously.66 BHK-21/WI-2 cells were plated into a 6 well plate ...
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bioRxiv - Immunology 2022Quote: VSV vectors pseudotyped with S of SARS-CoV-2 were generated in BHK-21/WI-2 cells using a the ΔG-GFP (G*ΔG-GFP) rVSV kit (Kerafast, Boston, MA, USA) and S sequences cloned into an expression plasmid under the control of the CMV promoter as described previously [51] ...
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bioRxiv - Microbiology 2021Quote: ... The blots were then incubated over night at 4 °C with primary antibody solution (all antibodies were diluted in PBS-T containing 5 % skim milk; mouse anti-HA tag [Sigma-Aldrich, H3663, 1:2,500] or VSV matrix protein [Kerafast, EB0011, 1:2,500]). Following this incubation ...
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bioRxiv - Microbiology 2021Quote: ... Pseudoviruses were generated in BHK-21/WI-2 cells using a pseudotyped ΔG-GFP (G*ΔG-GFP) rVSV (Kerafast, Boston, MA, USA) in addition to spike sequences cloned into the paT7- Spike plasmid as described previously (21) ...
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bioRxiv - Microbiology 2021Quote: ... or BHK-21/WI-2 (Kerafast) cells were transfected with SARS-CoV-2 Spike expression plasmid (pTT5 SARS-CoV-2 SΔ21) ...
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bioRxiv - Biochemistry 2023Quote: ... 20% glycerol] and 2 µL of Marathon (Kerafast) were added to the solution ...
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bioRxiv - Microbiology 2020Quote: ... and was ligated into the pVSV-FL+(2) vector (Kerafast), pre-cut by the same enzymes to remove the VSV-G gene ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells (Kerafast) were transformed with the expression vector and grown at 37 °C in LB medium with 0.4% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... A2EN cells (Kerafast) were propagated in keratinocyte-serum free media (K-SFM ...
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bioRxiv - Cell Biology 2023Quote: ... coli cells (Kerafast) and Get1 was expressed in E ...
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bioRxiv - Immunology 2023Quote: ... A2EN cells (Kerafast) were grown in phenol red-free serum-free medium (EpiLife ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... UNCN1T cells (a human bronchial epithelial cell line; Kerafast; cat# ENC011) were cultured using BEGM media (Bronchial Epithelial Cell Growth Medium ...
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bioRxiv - Cell Biology 2023Quote: ... coli LOBSTR cells (Kerafast), and grown in 2XYT media supplemented with 10 μM ZnCl2 ...
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bioRxiv - Immunology 2021Quote: ... and one day later infected (MOI = 2) with GFP-encoding VSVΔG backbone virus (purchased from Kerafast). Two hours later ...
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bioRxiv - Immunology 2022Quote: ... mouse colorectal cancer MC38 cells and melanoma B16 cells were purchased from Kerafast, luciferase-reporter Jurkat-dual cells were purchased from Invivogen ...
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bioRxiv - Immunology 2022Quote: ... mouse colorectal cancer MC38 cells and melanoma B16 cells were purchased from Kerafast. luciferase-reporter THP-1 dual cells were purchased from Invivogen ...
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bioRxiv - Neuroscience 2020Quote: ... HEK293T cells (ATCC; CRL-11268) and IMG (Immortalized adult mouse microglia) cells (Kerafast; EF4001) were grown in DMEM medium containing 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... and were infected 2 days post-transfection with >107 infectious units/well of VSVΔG-GFP (Kerafast, (22)) ...
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bioRxiv - Immunology 2021Quote: ... 2021) and one day later infected (MOI = 2) with GFP-encoding VSVΔG backbone virus (purchased from Kerafast). Two hours later ...
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bioRxiv - Bioengineering 2020Quote: ... MC38 cells purchased from Kerafast, Inc ...
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bioRxiv - Biochemistry 2021Quote: ... coli LOBSTR cells (EC1001, KeraFast) were used for the expression of PRAS40 ...
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bioRxiv - Immunology 2020Quote: ... HEK293T/17 monolayers transfected to express SARS-CoV-2 S truncated of the C-terminal cytoplasmic 19 amino acids (SARS-CoV-2-S-CΔ19) were inoculated with VSVΔG-GFP vector (rescued from pVSVΔG-GFP plasmid expression vector; Kerafast Inc.). After incubation for 1 h at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... at a density of one cell/per well with pre-coated EL4-B5 feeder cells (Kerafast). The feeder cells were irradiated with 50 Gy in a gamma radiation chamber before use ...
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bioRxiv - Physiology 2019Quote: MLO-Y4 osteocyte like cells (Kerafast) [63] were cultured ...
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bioRxiv - Microbiology 2021Quote: ... coli LOBSTR-BL21(DE3) cells (Kerafast) harbouring the pET28a-LdtE-his plasmid were grown in 2 L of LB-autoinduction medium (LB supplemented with 0.5% glycerol ...
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bioRxiv - Bioengineering 2021Quote: MLO-Y4 osteocyte like cells (Kerafast) [57] were maintained as previously described [58] in α-MEM growth medium with 2.5% fetal bovine serum (FBS) ...
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bioRxiv - Cancer Biology 2021Quote: ... MC38 cells (Kerafast, Boston MA, USA), was maintained in DMEM with 10% FBS ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21(DE3) LoBSTr cells (Kerafast) to generate NEC-CBM ...
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bioRxiv - Immunology 2023Quote: LUVA cells (45; Kerafast, #EG1701-FP) are derived from untransformed CD34+ enriched mononuclear hematopoietic progenitor cells ...