-
No products found
because this supplier's products are not listed.
Shubhangini Tiwari, et al.,
bioRxiv - Neuroscience 2022
Quote:
... The cDNA was obtained by RT-PCR and amplified through PCR using respective primers (OriGene Technologies) followed by agarose gel electrophoresis to assess the gene expression ...
-
No products found
because this supplier's products are not listed.
Jessica Avila Lopez, et al.,
bioRxiv - Neuroscience 2023
Quote:
... mRNA was reverse transcribed into single-stranded cDNA using the SensiFAST cDNA Synthesis Kit for RT-PCR (Cat. No BIO-65054, FroggaBio). After conversion to cDNA ...
-
No products found
because this supplier's products are not listed.
Leelavathi N Madhu, et al.,
bioRxiv - Neuroscience 2024
Quote:
... The qRT-PCR was performed using RT² SYBR Green qPCR Mastermix and Primer mix (GeneCopoeia, Rockville, Maryland, USA) for measuring human microglial gene expression in iMicroglia (tmem119 ...
-
No products found
because this supplier's products are not listed.
Line Jensen Ostenfeld, et al.,
bioRxiv - Microbiology 2022
Quote:
45μl PCR product was purified with Gene Clean Turbo for PCR kit (MP Biomedicals) according to the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Marcus V Merfa, et al.,
bioRxiv - Microbiology 2022
Quote:
... PCR products were purified using the Gel/PCR DNA Fragments Extraction kit (IBI Scientific). Correct cloning and amino acid exchanges in all constructs were confirmed by Sanger sequencing (Sequetech Corporation ...
-
No products found
because this supplier's products are not listed.
Samantha J. England, et al.,
bioRxiv - Genetics 2020
Quote:
... The PCR product was purified using EZ-10 Spin Column PCR Products Purification Kit (BS664, Bio Basic) and eluted in 30 µl sterile water prior to sequencing.
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No products found
because this supplier's products are not listed.
Marisa Connell, Yonggong Xie, Rui Chen, Sijun Zhu,
bioRxiv - Cell Biology 2021
Quote:
... HRP-Rt (Jackson Immunoresearch; 1:2000), and HRP-Rb (Cell Signaling Technology ...
-
No products found
because this supplier's products are not listed.
Virginia Andrade, et al.,
bioRxiv - Cell Biology 2022
Quote:
... 3 % milk at RT and revealed by LI-COR (Biosciences).
-
No products found
because this supplier's products are not listed.
Bailey Layish, et al.,
bioRxiv - Microbiology 2023
Quote:
... Cells were monitored quarterly for retroviral contamination by SYBR-Green based PCR RT assay (43, 44) and tested for mycoplasma contamination by MycoStrip® detection kit (InvivoGen). Derivatives of HeLa and HT1080 cells containing doxycycline-inducible Mx2 ...
-
No products found
because this supplier's products are not listed.
Linda Scaramuzza, et al.,
bioRxiv - Neuroscience 2020
Quote:
... EXcellence RT software (Olympus) was used to set up experiments and samples’ recordings ...
-
No products found
because this supplier's products are not listed.
Klaudyna Borewicz, et al.,
bioRxiv - Microbiology 2024
Quote:
... PCR products were then purified with the HighPrep® PCR kit (MagBio Genomics, Gaithersburg, MD, USA) and concentrations of indexed cDNA were measured using the Qubit®dsDNA BR Assay Kit (Invitrogen ...
-
No products found
because this supplier's products are not listed.
Michael S. Breen, et al.,
bioRxiv - Neuroscience 2019
Quote:
... The e-Myco Mycoplasma PCR Detection Kit (Bulldog Bio, 25233) and the MycoAlert Mycoplasma Detection Kit (Lonza ...
-
No products found
because this supplier's products are not listed.
Uxoa Fernandez-Pelayo, et al.,
bioRxiv - Cell Biology 2024
Quote:
... using the Venor Gem Classic Mycoplasma PCR Detection Kit (Minerva Biolabs).
-
No products found
because this supplier's products are not listed.
Abeba Zewdu, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... supernatant was collected and centrifuged (3000 rpm, 5 min, RT) and Secreted IL6 was measured using the Quantikine® ELISA kit (R&D Systems), following manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Gloria Ha, et al.,
bioRxiv - Cell Biology 2024
Quote:
... Cells were regularly validated as mycoplasma free by a PCR-based mycoplasma detection kit (Southern Biotech).
-
No products found
because this supplier's products are not listed.
Bence Daniel, et al.,
bioRxiv - Genomics 2024
Quote:
... Bead-bound libraries were amplified with the PCR reagents from the Ovation Ultralow Library V2 Kit (Tecan). Libraries were quantified and sequenced on a Novaseq 6000 with 2×150bp configuration.
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No products found
because this supplier's products are not listed.
Nadine Honke, et al.,
bioRxiv - Immunology 2020
Quote:
... at RT and permeabilized with InsidePerm at 4°C (Miltenyi Biotec) followed by incubation with anti-IL-10-PE-conjugated antibody (Miltenyi Biotec) ...
-
No products found
because this supplier's products are not listed.
Giulia Iacono, et al.,
bioRxiv - Cell Biology 2023
Quote:
... RT-reactions and RNA sequencing was performed by GENEWIZ (NJ, USA) using random hexamers ...
-
No products found
because this supplier's products are not listed.
Andrea S. Baez-Gonzalez, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Quantitative RT-PCR (qPCR) was performed with FastSYBR mixture (CoWin Biosciences) on the AriaMx Real-Time PCR System (Agilent Technologies ...
-
No products found
because this supplier's products are not listed.
Ana Martín-Leal, et al.,
bioRxiv - Immunology 2020
Quote:
... Quantitative RT-PCR was performed using FluoCycle II SYBR Master Mix (EuroClone) with specific primers (Supplementary Table S1 ...
-
No products found
because this supplier's products are not listed.
Juhee Son, et al.,
bioRxiv - Microbiology 2021
Quote:
... All piglets were confirmed negative for TGEV by RT-PCR and ELISA (IDEXX, USA). The pigs were randomly separated into three groups ...
-
No products found
because this supplier's products are not listed.
Sushama Telwatte, et al.,
bioRxiv - Microbiology 2021
Quote:
... The log-linear relationship between viral load measured by RT-PCR (Abbott Real Time SARS-CoV-2 assay) and RT-ddPCR was determined using GraphPad Prism (version 8.4.1).
-
No products found
because this supplier's products are not listed.
Debjani Pal, et al.,
bioRxiv - Cell Biology 2021
Quote:
... and reverse transcription was performed using the SuperScript II RT kit (Integrative DNA Technologies) with total RNA (1 μg ...
-
No products found
because this supplier's products are not listed.
Haichuan Wang, et al.,
bioRxiv - Genomics 2022
Quote:
... the pooled PCR products were purified using sbeadex PCR clean-up kits (LGC, Biosearch Technologies) by following the manufacturer’s instructions to purify the PCR band at 286 bp ...
-
No products found
because this supplier's products are not listed.
Heather J. Painter, et al.,
bioRxiv - Microbiology 2020
Quote:
... Mycoplasma PCR Detection kit (Boca Scientific; cat. no. 25235). If parasite cultures tested positive for Mycoplasma ...
-
No products found
because this supplier's products are not listed.
Dawei Qiu, et al.,
bioRxiv - Cell Biology 2024
Quote:
... 1 μg RNA was analyzed by using a TaqMan One Step RT-qPCR Kit (Solarbio, T2210) on LightCycle 96 Instrument (Roche ...
-
No products found
because this supplier's products are not listed.
Wakana Sato, et al.,
bioRxiv - Synthetic Biology 2021
Quote:
... and then purified with GenCatch PCR Purification Kit (Epoch Life Science, Inc., No. 2360250). pKDsgRNA-trml was transformed into NEB® 5-alpha Competent E ...
-
No products found
because this supplier's products are not listed.
Luis C. Santos, et al.,
bioRxiv - Cell Biology 2021
Quote:
mgWAT from RT or cold exposed mice (Jackson Laboratory, #000664) was fixed in 10% formalin overnight ...
-
No products found
because this supplier's products are not listed.
Casper Sahl Poulsen, et al.,
bioRxiv - Microbiology 2021
Quote:
... and followed by amplification-free library preparation using NEXTflex PCR Free DNA prep kit (Bioo Scientific) according to standard manufacturer protocols ...
-
No products found
because this supplier's products are not listed.
Paul C Campbell, Christopher L de Graffenried,
bioRxiv - Microbiology 2023
Quote:
... sgRNA PCRs were pooled and purified with a Zymo Research DNA Clean and Concentrator kit (Genesee Scientific) and resuspended in 25 μL of Ultra-Pure Water (Genesee) ...
-
No products found
because this supplier's products are not listed.
Ana Elena Pérez-Cobas, et al.,
bioRxiv - Microbiology 2019
Quote:
... pneumophila in the samples was detected by diagnostic PCR using primers and probes of the R-DiaLegTM kit (Diagenode, Belgium). The PCRs were prepared in a final volume of 20 μL by adding the Master Mix 5X (TaqMan Probe LC2.0 ...
-
No products found
because this supplier's products are not listed.
Rajagopal Ayana, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The SMRT sequencing was performed on the PacBio Sequel at the Genomics Core at KU Leuven (Belgium) using the Clontech SMARTer PCR cDNA Synthesis Kit (recommended by Pacbio). Multiple parallel PCR reactions were set after the first strand synthesis employing the PrimeSTAR GXL DNA Polymerase kit ...
-
No products found
because this supplier's products are not listed.
Robert Edwards, et al.,
bioRxiv - Microbiology 2019
Quote:
... Samples were tested by PCR and all PCR products were sequenced by Eton Bioscience, San Diego ...
-
No products found
because this supplier's products are not listed.
Hironori Abe, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... at RT for 30 min before incubation with anti-RNF8 antibody (Proteintech, 14112-1-AP), diluted 1/2000 in Tris-buffered saline containing 0.1% Tween 20 detergent (TBST) ...
-
No products found
because this supplier's products are not listed.
Seungyoul Oh, et al.,
bioRxiv - Immunology 2022
Quote:
... in Direct PCR Lysis Buffer (Viagen) at 55° C for 2h ...
-
No products found
because this supplier's products are not listed.
Ian C Tobias, et al.,
bioRxiv - Genetics 2023
Quote:
... All cultures were confirmed to be free of mycoplasma contamination during routine testing with the Mycoplasma PCR Detection Kit (BioVision Inc. K1476-100).
-
No products found
because this supplier's products are not listed.
Davide Monteferrario, et al.,
bioRxiv - Bioengineering 2024
Quote:
Chromatin immunoprecipitation followed by real-time quantitative PCR (ChIP-qPCR) was performed using the ChIP-IT PBMC kit (53042; Active Motif, Carlsbad CA) according to manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Zhaohua Guo, et al.,
bioRxiv - Neuroscience 2019
Quote:
Whole cell patch-clamp recordings were performed at RT with a MultiClamp 700B amplifier (Molecular Devices). The recording chamber was perfused with Tyrode’s solution (0.5 ml/min) ...
-
No products found
because this supplier's products are not listed.
Monique G.P. van der Wijst, et al.,
bioRxiv - Immunology 2021
Quote:
Peripheral blood mononuclear cells (PBMCs) were isolated at RT using SepMate PBMC Isolation Tubes (STEMCELL Technologies) by the UCSF Biospecimen Resource Program ...
-
No products found
because this supplier's products are not listed.
Susan Paton, et al.,
bioRxiv - Microbiology 2021
Quote:
... RT-PCR was performed using the VIASURE SARS-CoV-2 Real Time PCR Detection Kit (Viasure; CerTest Biotec, Zaragoza, Spain), following the methods provided ...
-
No products found
because this supplier's products are not listed.
Takenori Kanai, et al.,
bioRxiv - Cell Biology 2019
Quote:
... The RT-PCR products were separated on 2% agarose gel (NuSieve; FMC, Rockland, ME, USA) and visualized by Syber Green (Takara) ...
-
No products found
because this supplier's products are not listed.
Alexia Carré, et al.,
bioRxiv - Immunology 2023
Quote:
... INS KO was validated by RT-PCR and by INS ELISA (Mercodia, #10-1113-01) according to the manufacturer’s protocol.
-
No products found
because this supplier's products are not listed.
Hirokazu Kimura, et al.,
bioRxiv - Genetics 2023
Quote:
... and the PCR-based MycoDtect kit (Greiner Bio-One ...
-
No products found
because this supplier's products are not listed.
Will Macnair, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Fluorophore reaction was performed using OPAL 570 and OPAL 650 reaction kits for 10 min at RT (Akoya Biosciences ...
-
No products found
because this supplier's products are not listed.
Anissa A. Widjaja, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Telomere length and mitochondrial copy number for HCFs were evaluated by RT-qPCR with the Relative Human Telomere Length Quantification qPCR Assay Kit (8908, ScienCell) and Relative Human Mitochondrial DNA copy number Length Quantification qPCR Assay Kit (8938 ...
-
A component of the Papain Dissociation System, for use in the tissue dissociation method of...
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Sumit Handa, et al.,
bioRxiv - Biochemistry 2020
Quote:
Reverse transcription reactions with Moloney Murine Leukemia Virus (MMLV) RT (BioBharati Life Sciences Pvt. Ltd) and HIV RT (Worthington Biochemical) were carried out according to the manufacturer’s directions using primer P5 (Table S1) ...
-
No products found
because this supplier's products are not listed.
Joseph I Aubee, et al.,
bioRxiv - Microbiology 2024
Quote:
... All PCR reactions were purified using The Column-PureTM Clean-Up Kit (Lamda Biotech), according to the manufacturer’s instructions (Lamda Biotech).
-
No products found
because this supplier's products are not listed.
Johanna Hol Fosse, et al.,
bioRxiv - Microbiology 2023
Quote:
... treated with blocking buffer (Background sniper, Biocare Medical, 30 min, RT), incubated with mouse IgG1 specific to ISAV HE (clone 3H6F8 [52] ...
-
No products found
because this supplier's products are not listed.
Aljawharah Alrubayyi, et al.,
bioRxiv - Immunology 2021
Quote:
... followed by two washes with PBS and then incubated for 2 hr at RT with biotinylated anti-IFN-γ (1 μg/mL; clone mAb-7B6-1; Mabtech). After six further washes ...
-
No products found
because this supplier's products are not listed.
Joana Rajão-Saraiva, et al.,
bioRxiv - Neuroscience 2022
Quote:
... membranes were incubated with horseradish peroxidase (HRP)—conjugated anti-mouse or anti-rabbit secondary antibodies for 1 h at RT: Goat Anti-Mouse IgG HRP (1:4000, 10004302, Cayman Chemicals) or Goat Anti-Rabbit IgG HRP (1:10.000 ...