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Citations for PacBio :
1 - 50 of 264 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The SMRT sequencing was performed on the PacBio Sequel at the Genomics Core at KU Leuven (Belgium) using the Clontech SMARTer PCR cDNA Synthesis Kit (recommended by Pacbio). Multiple parallel PCR reactions were set after the first strand synthesis employing the PrimeSTAR GXL DNA Polymerase kit ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified using AMPure®PB beads (PacBio), following manufacturer’s guidelines ...
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bioRxiv - Microbiology 2022Quote: ... PCR product pools were purified using AMPure®PB beads (PacBio). Sequencing was performed at the Functional Genomic Center Zürich ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Using SMRTBell template Prep Kit 1.0 and SMRTbell Barcoded Adaptater kit 8A or 8B kits (PacBio), samples (1 µg ...
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bioRxiv - Bioengineering 2023Quote: ... 2 weeks later and subjected to large deletions detection (PCR and PacBio sequencing). Single cells were isolated from bulk edited cells into 96-well plates 2 weeks after electroporation and genotyped 2 weeks after isolation ...
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bioRxiv - Genomics 2020Quote: ... Sequencing was performed on a PacBio Sequel instrument using the Sequel® Binding Kit and Internal control Kit 3.0 and the Sequel® Sequencing Kit 3.0 (PacBio). Long read data was assembled using the HGAP4 assembler.
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bioRxiv - Genomics 2023Quote: ... and the Express Template Prep Kit v2 and SMRTbell Cleanup Kit v2 (PacBio). The library was size-selected on a PippinHT instrument (Sage Science ...
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bioRxiv - Microbiology 2021Quote: ... PCR chemicals and conditions (27 cycles) were performed as suggested by Pacific Biosciences (PacBio, 2020). PCR products were visualized on an 1 % (w/v ...
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bioRxiv - Microbiology 2021Quote: ... SMRTbell libraries were prepared using the SMRTbell Express Template Prep Kit 2.0 (PacBio kit). Long read data were assembled by canu 2.1.1(44) ...
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bioRxiv - Microbiology 2024Quote: ... The amplicon library was contracted with the SMRTbell® prep kit 3.0 library prep kit (PacBio) following the protocol ...
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bioRxiv - Plant Biology 2021Quote: ... A Template Prep Kit (PacBio, USA) was used to remove failed ligation products ...
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bioRxiv - Microbiology 2019Quote: ... The SMARTbell Template Prep Kit (PacBio) was used to add adaptors to amplicons ...
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bioRxiv - Microbiology 2019Quote: ... the 20 kb BluePippin kit (PacBio) was used and DNA libraries were sequenced on a PacBio Sequel System with Sequel Sequencing chemistry 2.1 ...
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bioRxiv - Developmental Biology 2023Quote: ... Iso-Seq Express Oligo Kit (PacBio) and SMRTbell Express Template Prep Kit 2.0 (PacBio) ...
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bioRxiv - Plant Biology 2019Quote: ... The DNA/Polymerase Binding Kit P6 (PacBio) was used for DNA synthesis after the sequencing primer annealed to the SMRTbell template ...
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bioRxiv - Genomics 2021Quote: ... Using SMRTBell template Prep Kit 1.0 (PacBio), a DNA and END damage repair step was performed on 5 μg of sample ...
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bioRxiv - Cancer Biology 2022Quote: ... Sequencing Kit 2.0 (PacBio, 101-389-001) and Instrument Chemistry Bundle Version 11.0 were used ...
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bioRxiv - Genomics 2023Quote: ... Using SMRTBell template Prep Kit 1.0 (PacBio), a DNA and END damage repair step was performed on 15µg of unshared sample ...
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bioRxiv - Genetics 2019Quote: ... Sheared DNA was barcoded and multiplexed for PacBio sequencing using Template Prep Kit 1.0-SPv3 and Barcoded Adapter Kit 8A (PacBio). Genomic DNA was sequenced using the PacBio Sequel system using version 3.0 sequencing reagents (PacBio ...
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bioRxiv - Genomics 2023Quote: ... An Iso-Seq cDNA library was generated with the Iso-Seq Express Oligo Kit and the SMRTbell Express Template Prep Kit 2.0 (PacBio). The Iso-Seq library was sequenced using a single SMRTcell on a Sequel I at the D-BSSE (Basel ...
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bioRxiv - Genomics 2020Quote: ... HMW DNA was used to generate HiFi libraries via the SMRTbell Express Template Prep Kit v2 and SMRTbell Enzyme Clean Up kits (PacBio). Size selection was performed with SageELF (Sage Science) ...
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bioRxiv - Genomics 2023Quote: ... The sheared material was subjected to SMRTbell library preparation using the Express Template Prep Kit v2 and SMRTbell Cleanup Kit v2 (PacBio). After checking for size and quantity ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted using either the Nanobind Tissue Big DNA kit (cell line and brain) or the Nanobind HT 1ml blood kit (blood) (PacBio). For the cell line and blood samples ...
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bioRxiv - Microbiology 2021Quote: The mCherry-E3L integration sites in the purified HGT virus genomes were located by inverse PCR amplification and by PacBio sequencing ...
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bioRxiv - Microbiology 2021Quote: ... using the Sequel II Sequencing Kit 2.0 (PacBio). Sequencing data quality were checked ...
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bioRxiv - Genetics 2021Quote: ... and the Express Library Prep Kit v2 (PacBio), size selecting on the SageELF platform (Sage Science ...
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bioRxiv - Plant Biology 2022Quote: ... and SMRTbell Express Template Prep Kit 2.0 (PacBio). Thereafter ...
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bioRxiv - Genetics 2022Quote: ... The SMRTBell® Template Prep Kit 1.0 (PacBio) was then used to prepare 14-kilobase libraries ...
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bioRxiv - Genomics 2023Quote: ... using the Nanobind CBB DNA extraction kit (PacBio), according to the manufacturer’s instructions with modifications ...
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bioRxiv - Developmental Biology 2023Quote: ... and SMRTbell Express Template Prep Kit 2.0 (PacBio). Samples were sequenced using PacBio Sequel II 8M SMRT cell (version 2.1 ...
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bioRxiv - Genomics 2024Quote: ... The SMRTbell Express Template Prep Kit 2.0 (PacBio) was used to construct the PacBio sequencing library ...
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bioRxiv - Genomics 2022Quote: ... Barcoded PacBio SMRTBell adapters from the PacBio 8A barcoded adapter kit (Barcoded Overhang Adapter Kit - 8A, PacBio PN: 101-628-400) were added to ligation products following an adapted version of the PacBio Microbial Multiplexing PacBio protocol (Part Number 101-696-100 Version 07 (July 2020) ...
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bioRxiv - Microbiology 2021Quote: ... using the Sequel II Sequencing Kit 1.0 (PacBio, USA) with the Sequel II SMRT Cell 8M (PacBio ...
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bioRxiv - Genomics 2022Quote: ... It’s recommended to use the barcode kit by PacBio to achieve the best sequencing result ...
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bioRxiv - Genomics 2023Quote: ... and Iso-Seq Express Oligo Kit (Pacific Biosciences (PacBio), CA ...
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bioRxiv - Genomics 2023Quote: ... or Circulomics Nanobind Tissue kit (Pacbio 102-302-100). The linked read library was prepared using the Chromium Genome Reagent Kit v2 following the protocol provided by 10x Genomics ...
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bioRxiv - Genomics 2023Quote: ... The Sequel® II Binding Kit 2.2 (PacBio, USA) was used to prepare the libraries for loading ...
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bioRxiv - Genomics 2023Quote: ... using the Sequel® II Sequencing Kit 2.0 (PacBio) on the Sequel® II Sequencer (PacBio).
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bioRxiv - Bioengineering 2021Quote: ... The genomic region containing integrated BiTE sequences amplified by PCR were gel purified and analyzed by long read amplicon sequencing (PacBio SMRT®). The circular consensus reads (∼3 million CCS reads ...
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bioRxiv - Genomics 2021Quote: HiFi SMRTbell Libraries of FCtx1 and FCtx2 were constructed according to the manufacturer’s manual (entitled “Preparing HiFi SMRTbell Libraries using SMRTbell Express Template Prep Kit 2.0”) by using SMRTbell Express Template Prep Kit 2.0 (PacBio, catalog no. 100-938-900). For both samples ...
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bioRxiv - Genomics 2023Quote: ... a PacBio SMRT Bell continuous long read (CLR) library was prepared using the SMRTbell Express Prep kit v3.0 kit (Pacific Biosciences – PacBio, Menlo Park, CA, USA) and sequenced on the PacBio Sequel IIe platform ...
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bioRxiv - Microbiology 2021Quote: ... library was prepared using SMRTbell template prep kit (PacBio, USA) according to manufacturer instructions ...
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bioRxiv - Plant Biology 2022Quote: ... The library was prepared using the SMRTBell express Kit (PacBio) and sequenced on a PacBio Sequel platform (PacBio ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and nuclease treated with SMRTbell enzyme cleanup kit 1.0 (PacBio). The resulting libraries were quantified by fluorescence (Invitrogen Qubit 3.0 ...
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bioRxiv - Neuroscience 2024Quote: ... with the Nanobind CBB Kit (PacBio, SKU 102-301-900) and the UHMW DNA Aux Kit (Circulomics/PacBio ...
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bioRxiv - Microbiology 2019Quote: ... a second round of PCR was done with distinct barcoded universal F/R primers as provided by the manufacturer (PacBio, Menlo Park, CA). SMRTbell Library preparation and SMRT sequencing on a PacBio Sequel System was also done according to manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: The following section of the sequencing preparation was completed using kit components from the MAS-Seq for 10x Single Cell 3’ kit (PacBio, cat. no. 102-659-600), as well as individually created oligos ...
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bioRxiv - Cancer Biology 2024Quote: The following section of the sequencing preparation was completed using kit components from the MAS-Seq for 10x Single Cell 3’ kit (PacBio, cat. no. 102-659-600), as well as individually created oligos ...
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bioRxiv - Biochemistry 2020Quote: ... SMRTbell Template Prep Kit 1.0 (PN 100-259-100) from PacBio was used for preparing the libraries ...
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bioRxiv - Genomics 2021Quote: ... Libraries were prepared using the SMRTbell Template Prep Kit 1.0 (PacBio), loaded using either magbeads or diffusion ...