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Citations for SouthernBiotech :
1 - 50 of 50 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cells were regularly validated as mycoplasma free by a PCR-based mycoplasma detection kit (Southern Biotech).
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bioRxiv - Microbiology 2024Quote: ... Cells were routinely confirmed to be mycoplasma free by conducting PCRs using a mycoplasma detection kit (SouthernBiotech).
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bioRxiv - Cell Biology 2020Quote: ... for 30 min at RT and cover-slipped with Fluoromount (SouthernBiotech). Paraffin sections (3 µm ...
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bioRxiv - Immunology 2023Quote: ... Membranes were washed with TBST (1x, 5 min., RT) and incubated with goat anti-rabbit IgG- HRP (Southern Biotech #1021-05, 10 mL, 1:5,000, 1 h, RT). Membranes were washed with TBST (3x ...
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bioRxiv - Physiology 2020Quote: ... Cells were also regularly checked for mycoplasma contamination using a PCR based mycoplasma detection kit from SouthernBiotech (Birmingham, AL) and were confirmed to be negative ...
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bioRxiv - Immunology 2020Quote: ... for 30 min RT before being mounting using Fluoromount-G containing DAPI (SouthernBiotech). Fluorescent slides were imaged using an EVOS™ FL Imaging System (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: ... incubated in Hoechst for 5 min at RT and mounted with coverslips using Fluoromount-G (Southern Biotech). For wax preservation ...
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bioRxiv - Immunology 2020Quote: ... or with goat anti-mouse IgM-HRP (Southern Biotech #1021-05, 10 mL, 1:5,000, 1 h, RT) for detection of M86 mAb ...
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bioRxiv - Developmental Biology 2020Quote: ... slides were washed in PBS at RT 3×5min and mounted in Fluoromount-G (SouthernBiotech, Cat: 0100-01) before confocal imaging.
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bioRxiv - Microbiology 2022Quote: ... then incubated in 0.1% TBST containing 1:4000 goat anti-rabbit antibody conjugated to HRP for 1 h at RT (SouthernBiotech).
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bioRxiv - Cancer Biology 2023Quote: ... the coverslips were fixed in 4% paraformaldehyde for 20 min at RT and mounted in DAPI Fluoromount-G (SouthernBiotech, USA). Slides were subjected to confocal imaging in a Zeiss LSM 700 microscope (Zeiss ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated with secondary antibodies conjugated to Alexa Fluor for one hour at RT and washed with PBS before mounting onto microscope slides using fluoromount-g (Southern Biotech).
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bioRxiv - Systems Biology 2019Quote: ... to stain DNA for 10 min at RT before they were covered with coverslips using Fluoromount-G® (SouthernBiotech; 0100-01). Samples were dried and fluorescence was analysed with a LSM 710 laser scanning microscope (Carl Zeiss ...
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bioRxiv - Bioengineering 2021Quote: ... arrays were incubated for an additional 2 h at RT and 60 rpm with goat anti-mouse IgG conjugated to HRP (Southern Biotech) diluted 1:5000 in blocking solution ...
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bioRxiv - Genetics 2023Quote: ... and verified by PCR to be Mycoplasma free (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... was added to each well and incubated at RT for 3 minutes and the reaction was terminated with 50 μL of TMB Stop solution (SouthernBiotech, 0412-01). Absorbance at 450 nm (OD450 ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were stained using the ACD supplied DAPI stain for 30 s at RT and the slides were mounted using Fluromount-G (SouthernBiotech, # 0100-01). ACD’s 4-plex positive (#321811 ...
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bioRxiv - Immunology 2023Quote: ... Plates were then incubated for 90min at RT with goat anti-mouse IgG conjugated to horseradish peroxidase (HRP) (Southern Biotech, #1030-05) at 1:5000 dilution in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... Coverslips were washed thoroughly with 1xPBS at RT 3x 5min each and mounted in mounting medium containing DAPI (DAPI Fluoromount-G™ - Southern Biotech). Antibodies used and concentrations can be found in the Key Resources Table (KRT).
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bioRxiv - Cell Biology 2022Quote: ... Cells were routinely tested for mycoplasma contamination by PCR (Southern Biotech).
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bioRxiv - Cell Biology 2020Quote: ... Then samples were washed three times with 0.1% Triton X-100 in PBS for 5 minutes and were incubated 30 minutes at RT with the following secondary antibody diluted 1:200: goat anti-rabbit Alexa Fluor 488 (Southern Biotech 4050-30), goat anti-mouse Alexa Fluor 488 (Southern Biotech 1030-30 ...
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bioRxiv - Microbiology 2021Quote: ... secondary antibody at RT for 2h and stained with 4’,6-diamidino-2-phenylindole (DAPI) before mounting onto slides with Fluoromount-G (Southern Biotech, Birmingham, AL).
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bioRxiv - Immunology 2020Quote: ... with excess of PBS-T before being incubated for one hour at RT with an alkaline phosphatase-conjugated goat anti-human IgG (Southern Biotech, Birmingham, AL), diluted 3000 × in PBS-T ...
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bioRxiv - Immunology 2020Quote: ... the plates were first washed 5 times with PBS-T and then incubated for 1 hour at RT with HRP-conjugated anti-human IgG (Fc-specific, clone JDC-10, Southern Biotech, Birmingham, AL) diluted 3000 × in PBS-T ...
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bioRxiv - Immunology 2020Quote: ... the plates were washed at least 5 times with PBS-T and then incubated for 1 hour at RT with HRP-conjugated anti-human IgG (Fc-specific, clone JDC-10, Southern Biotech, Birmingham, AL) diluted 3000 × in PBS-T ...
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bioRxiv - Microbiology 2023Quote: ... Coverslips were then incubated in secondary antibody in blocking buffer for 1 h at RT followed by 3×5 min washes in PBS and then mounted in Fluoromount G with DAPI (Southern Biotech, Birmingham, AL).
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bioRxiv - Cancer Biology 2020Quote: ... All cell lines were mycoplasma free as determined PCR assay (Southern Biotech).
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bioRxiv - Cell Biology 2023Quote: ... and incubated at RT for 1 h with the following HRP-conjugated secondary antibodies: goat anti-rabbit IgG conjugated to HRP (Southern Biotech, 4010-05; 1:5000), and goat anti-mouse IgG conjugated to HRP (Southern Biotech ...
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bioRxiv - Microbiology 2023Quote: ... All cell cultures were verified by PCR to be Mycoplasma-free (Southern Biotech).
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bioRxiv - Evolutionary Biology 2021Quote: ... Plates were incubated with serially diluted serum or gut content (50 μL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgA (Southern Biotech #0106-01, prepared in duplicates) and washed (5x ...
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bioRxiv - Immunology 2020Quote: ... Plates were incubated with serially diluted mouse sera (50 µL, PBS 1% BSA, wt/vol, 2 h, RT) and standard mouse IgG (Southern Biotech #0107-10, prepared in duplicates) and washed (5x ...
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bioRxiv - Cancer Biology 2019Quote: ApoScreen Annexin V-FITC kit from Southern Biotech was used and protocol was followed according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... All cells were maintained at 37 ◻C with 5% CO2 and were mycoplasma free as determined a PCR assay (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... Antibody isotyping kit was purchased from Southern Biotech (Cat#5300-05). FITC-conjugated streptavidin was purchased from BD Biosciences (Cat#554060) ...
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bioRxiv - Immunology 2022Quote: ... kit following manufacturer’s protocol for intracellular staining of FITC IgA (Southern Biotech), IgG2b ...
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bioRxiv - Molecular Biology 2022Quote: ... Mycoplasma test was performed using Mycoplasma detection kit by Southern Biotech (13100-01), according to manufacturer’s instructions.
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bioRxiv - Biophysics 2021Quote: ... Cell lines were tested for mycoplasma contamination via the Mycoplasma Detection Kit (Southern Biotech). Transient transfections were performed with Lipofectamine 2000 (Life Technologies) ...
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bioRxiv - Cell Biology 2020Quote: ... and were regularly checked for mycoplasma contamination using a Mycoplasma Detection Kit (SouthernBiotech; 131001). Cells were transfected with 3 μg of DNA using FuGENE HD (Promega ...
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bioRxiv - Immunology 2020Quote: ... Total serum immunoglobulin levels were detected using SBA Clonotyping System HRP kit (Southern Biotech).
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bioRxiv - Immunology 2022Quote: ... Class-switched serum immunoglobulin levels were detected using SBA Clonotyping System HRP kit (Southern Biotech).
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bioRxiv - Cancer Biology 2023Quote: ... followed by quantification of IgG1 abundance using the SBA Clonotyping System-HRP kit (SouthernBiotech; 5300-05) following the manufacturer’s instruction ...
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bioRxiv - Genomics 2023Quote: ... Cells were checked for mycoplasma contamination using the Mycoplasma detection kit (Southern Biotech, catalog #13100-01) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: The apoptosis level of PC12 cells was determined by ApoScreen Annexin V kit (SouthernBiotech, Birmingham, AL, USA) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Antibody subtype identification was performed using the SBA ClonotypingTM System/HRP Kit (Southern Biotech, Birmingham, AL, USA). To minimize the background impurities observed during immunoelectron microscopy ...
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bioRxiv - Microbiology 2019Quote: ... This cell line was routinely tested for mycoplasma contamination using a Mycoplasma Detection Kit (SouthernBiotech, Cat# 13100-01).
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bioRxiv - Immunology 2022Quote: Serum from uninfected mice was analyzed for total IgG1 using a mouse clonotyping kit and standards (Southern Biotech) according to manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2021Quote: ... Cells lines were authenticated and tested for mycoplasma contamination before use via the Mycoplasma Detection Kit (SouthernBiotech; 13100-01). Cells were maintained in a humidified atmosphere of 5% CO2 and 37 °C and were passaged every 2-3 days into 10 cm polystyrene coated plates (Eppendorf ...
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bioRxiv - Immunology 2021Quote: ... the same ELISA was performed but using the secondary antibodies from SBA Clonotyping System-HRP kit (SouthernBiotech, 5300-05, RRID:AB_2796080) diluted accordingly to manufacturer’s instructions.
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bioRxiv - Systems Biology 2023Quote: HEK293T was ordered from ATCC (ATCC CRL-11268) and tested negative for Mycoplasma using a Mycoplasma detection kit (Southern Biotech). HEK293T cells were cultured in Dulbecco minimal essential medium (DMEM ...
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bioRxiv - Cancer Biology 2020Quote: ... China).The apoptosis rate of U251 cells inoculated in the lower compartment was measured using a membrane-bound V-PE/7AAD kit (Southern Biotech, Birmingham, Ala, USA) after 12h.Then washing with phosphate buffer and centrifugation twice ...