-
No products found
because this supplier's products are not listed.
Luke M. Oltrogge, et al.,
bioRxiv - Biochemistry 2019
Quote:
Solution protein-protein binding was monitored by microscale thermophoresis (MST) with a Monolith NT.115 (Nanotemper). The target proteins were portions of the CsoS2 NTD fused to Superfolder GFP and used at a concentration of 50 nM ...
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No products found
because this supplier's products are not listed.
Suchitra Joshi, Cedric L Williams, Jaideep Kapur,
bioRxiv - Neuroscience 2022
Quote:
... the animals were habituated to the arena (45 × 45 × 40 cm, Harvard Apparatus) for 10 min for two days (habituation ...
-
No products found
because this supplier's products are not listed.
Keisuke Shoji, Yukihide Tomari,
bioRxiv - Genetics 2024
Quote:
... Small RNA libraries were prepared from ∼20–45 nt total RNAs using Small RNA Library Preparation kit (Illumina) and analyzed by Illumina HiSeq 2500 platform.
-
No products found
because this supplier's products are not listed.
Yury Bykov, et al.,
bioRxiv - Cell Biology 2019
Quote:
... incubated for 45 minutes with goat anti-rabbit 15nm gold antibody (EMS) diluted 1:20 in filtered blocking solution ...
-
No products found
because this supplier's products are not listed.
Tetyana P. Buzhdygan, et al.,
bioRxiv - Neuroscience 2020
Quote:
... spike protein subunit S2 or receptor binding domain (RBD) sequence of S1 (RayBiotech, Cat No 230-01101 ...
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No products found
because this supplier's products are not listed.
Özgen Deniz, et al.,
bioRxiv - Genetics 2020
Quote:
... or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913, Diagenode #C15410193). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
Julius Brandenburg, et al.,
bioRxiv - Immunology 2020
Quote:
... unspecific protein binding sites were blocked by incubating sections with PBS containing 10% normal donkey serum (Pan-Biotek), 2% BSA and 0.2% Triton-X100 for 1 hour ...
-
No products found
because this supplier's products are not listed.
Mark Wunderlich, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Anti-mouse and anti-human FcyR blocking antibodies were used to prevent non-specific binding (Miltenyi Biotec). 7-aminoactinomycin D (BD ...
-
No products found
because this supplier's products are not listed.
Elisa Avolio, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Cells were stained with DAPI and antibodies against double-stranded RNA (dsRNA, J2 10010200, Scicons) and SARS-CoV-2 nucleocapsid (N) protein (200-401-A50, Rockland) followed by appropriate secondary antibodies conjugated with Alexa Fluor dyes 586 and 647 ...
-
No products found
because this supplier's products are not listed.
Jordana Griffiths, et al.,
bioRxiv - Immunology 2020
Quote:
... For domain binding determination PE-labelled secondary anti-mouse Fc antibody (Jackson Laboratories) was added ...
-
No products found
because this supplier's products are not listed.
Patrik Risteski, et al.,
bioRxiv - Cell Biology 2024
Quote:
... and human anti-centromere protein antibody (Antibodies Incorporated). The secondary antibodies used were donkey anti-mouse IgG-Alexa Fluor 488 (Abcam) ...
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No products found
because this supplier's products are not listed.
Christopher von Beek, et al.,
bioRxiv - Immunology 2023
Quote:
LUVA cells (45; Kerafast, #EG1701-FP) are derived from untransformed CD34+ enriched mononuclear hematopoietic progenitor cells ...
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No products found
because this supplier's products are not listed.
Florent Ailloud, et al.,
bioRxiv - Microbiology 2023
Quote:
... Among the motifs not validated by PacBio data ...
-
No products found
because this supplier's products are not listed.
Jia Chen, et al.,
bioRxiv - Microbiology 2019
Quote:
... The blots were washed with PBS and incubated with primary antibodies (anti-EBV gH/gL (a rabbit polyclonal antiserum;1:200) (45) and anti-His tag antibody (OB05, Calbiochem, 1:1000) for His-tagged KSHV gL ...
-
No products found
because this supplier's products are not listed.
Thomas C. Harper, et al.,
bioRxiv - Immunology 2023
Quote:
... 20 ng/ml SHH (PeproTech, 100-45) and 10 μg/ml Angiotensin II (Sigma-Aldrich ...
-
No products found
because this supplier's products are not listed.
Lorena Sanchez Felipe, et al.,
bioRxiv - Microbiology 2020
Quote:
... and HRP conjugated secondary antibody (Protein Simple). Chemiluminescence signals were analyzed using Compass software (Protein Simple) ...
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No products found
because this supplier's products are not listed.
Alexandra Lucas, et al.,
bioRxiv - Neuroscience 2021
Quote:
... solution (Liberate Antibody Binding Solution, Polysciences, Inc ...
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No products found
because this supplier's products are not listed.
Angela M. Bosco-Lauth, et al.,
bioRxiv - Microbiology 2020
Quote:
... Positive control antibodies to the receptor-binding domain (RBD) and full-length spike protein were human MAb CR3022 antibody (Absolute Antibody, Oxford UK) and human IgG whole molecule (Jackson Immuno Research ...
-
No products found
because this supplier's products are not listed.
Yu-Le Wu, et al.,
bioRxiv - Biophysics 2021
Quote:
... Binding of primary antibody (Elys (catalog no. HPA031658, Atlas Antibodies, 1:50), Nup133 (catalog no ...
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No products found
because this supplier's products are not listed.
Magdalena Murr, et al.,
bioRxiv - Microbiology 2023
Quote:
... binding of peroxidase-conjugated species-specific secondary antibodies (Dianova) was detected by chemiluminescence substrate (Thermo Scientific ...
-
No products found
because this supplier's products are not listed.
Mahlon Collins, Yang Li, Robert Bowser,
bioRxiv - Neuroscience 2019
Quote:
... Tet-inducible EGFP-RBM45 stable cell lines (see below) were maintained in the presence of 50μg/ml Zeocin (Invivogen, San Diego, CA, USA). The expression of EGFP-RBM45 was induced with 1 μg/ml doxycycline hyclate (Sigma ...
-
No products found
because this supplier's products are not listed.
Maria Guirro, et al.,
bioRxiv - Systems Biology 2019
Quote:
... or a HFD (45% fat, 35% carbohydrate, and 20% protein; D12451, Research Diets, New Brunswick, USA). Two other groups were also fed either a LFD or a HFD for 11 weeks ...
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No products found
because this supplier's products are not listed.
Mina O. Seedhom, et al.,
bioRxiv - Immunology 2023
Quote:
... from 15% and 45% sucrose (MP Biomedicals) solutions in gradient buffer (20mM Tris-HCl pH 7.4 ...
-
No products found
because this supplier's products are not listed.
Robert W Hunter, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The binding sites of HRP-conjugated secondary antibodies were detected with Red Opal (1:100, AKOYA Biosciences) and a DAPI counterstain was applied (1:1000) ...
-
No products found
because this supplier's products are not listed.
Gang Wang, et al.,
bioRxiv - Microbiology 2021
Quote:
... and β-actin protein primary antibody (ABclonal, Lot#9100026001) respectively ...
-
No products found
because this supplier's products are not listed.
Matthew Prideaux, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Antibody against fatty acid binding protein 4 (FABP4) was purchased from ProSci, Inc ...
-
No products found
because this supplier's products are not listed.
Guojun Wu, et al.,
bioRxiv - Microbiology 2022
Quote:
... lipopolysaccharide-binding protein (Hycult Biotech, PA, USA), leptin (P&C ...
-
No products found
because this supplier's products are not listed.
Emilio Boada-Romero, et al.,
bioRxiv - Cell Biology 2023
Quote:
Lipid binding capacity of recombinant proteins or anti-PS antibody were analyzed using lipid arrays (Echelon Biosciences Inc ...
-
No products found
because this supplier's products are not listed.
James D. Hampton, et al.,
bioRxiv - Pharmacology and Toxicology 2021
Quote:
... the binding of the 10E4 antibody (clone F58-10E4, Amsbio) to sulfated HS can be quantitated using flow cytometry ...
-
No products found
because this supplier's products are not listed.
Miriam Kuhlmann, et al.,
bioRxiv - Immunology 2023
Quote:
Blocking of unspecific antibody binding was achieved with 2.4G2 (BioXCell) and dead cells were stained using the Zombie NIR™ Fixable Viability Kit (Biolegend) ...
-
No products found
because this supplier's products are not listed.
Parminder Kaur, et al.,
bioRxiv - Biophysics 2022
Quote:
... were titrated into the binding solution containing fluorescein-labeled DNA substrates (6 nM, 45 bp) using a Tecan Spark Multimode plate reader (Tecan Group Ltd) (40) ...
-
No products found
because this supplier's products are not listed.
Chengbao Ma, et al.,
bioRxiv - Microbiology 2023
Quote:
Protein binding kinetics was analyzed using BLI assays with the Octet RED96 instrument (Molecular Devices) following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Ina J. Andresen, et al.,
bioRxiv - Cell Biology 2020
Quote:
... RNA from the remaining 4 batches (batch 26, 27, 45 and 46) was isolated using the “Total RNA Purification Kit” (NORGEN BIOTEK CORP, Canada) with 40 ul elution ...
-
No products found
because this supplier's products are not listed.
Hongmei Qiao, et al.,
bioRxiv - Genomics 2020
Quote:
... AFP4/TMAC2 (ABI FIVE BINDING PROTEIN 4, AT3G02140), DOG1 (AT5G45830 ...
-
No products found
because this supplier's products are not listed.
Leslie M. Stevens, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... 0.325 μg/μl Maltose Binding Protein (MBP)-AviTag substrate (Avidity, L.L.C.), 8.3 mM ATP and 42 μM biotin ...
-
No products found
because this supplier's products are not listed.
Aswini Panigrahi, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... Galectin-3-binding protein ORF was subcloned from its source vector (Origene # RC204918) into bicistronic lentiviral transfer vector pHR-CMV-TetO2 3C-TwinStrep-IRES-EmGFP vector (Addgene ...
-
No products found
because this supplier's products are not listed.
Marina C. Nocente, et al.,
bioRxiv - Genomics 2023
Quote:
... We incubated the chromatin with either 45 µg antibodies against OCT4 (MBS420786, MyBioSource) or 45 µg of non-specific IgG as a control ...
-
No products found
because this supplier's products are not listed.
Chen Su, Yuxin Wang, Xiaoke Yang, Junyu Xiao,
bioRxiv - Biochemistry 2023
Quote:
... The bound proteins were eluted with the binding buffer supplemented with 10 mM desthiobiotin (IBA Lifesciences). The samples were analyzed by SDS-PAGE and detected by immunoblotting using antibodies to the strep tag (1:3,000 ...
-
No products found
because this supplier's products are not listed.
Marc Duque, et al.,
bioRxiv - Neuroscience 2021
Quote:
... A78416874 (20μM; Tocris, #4319, 45 min incubation) in 0.1% DMSO ...
-
No products found
because this supplier's products are not listed.
Maaike Allers, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... Purified protein was shipped for antibody production (custom polyclonal antibodies, Eurogentec). Rabbit polyclonal anti-Shp1 was purified in house by affinity purification ...
-
No products found
because this supplier's products are not listed.
Jamie Guenthoer, et al.,
bioRxiv - Immunology 2023
Quote:
... A SARS-CoV-2 nucleocapsid protein monoclonal antibody (Bioss Antibodies, clone 1C7) solution was prepared at 1 μg/mL and added to all wells for one hour at room temperature ...
-
No products found
because this supplier's products are not listed.
Takamasa Kudo, et al.,
bioRxiv - Systems Biology 2023
Quote:
... cells were blocked with 3% BSA in PBS for 45 minutes and then incubated with fluorophore direct-conjugated antibodies: Irf-1 conjugated with CF430 (Biotium #92118) with maleimide chemistry (Cell Signaling Technology #8478) ...
-
No products found
because this supplier's products are not listed.
Mariska S. Simpson, et al.,
bioRxiv - Cell Biology 2023
Quote:
... His-tagged proteins were affinity purified by binding to cobalt resin (GoldBio) equilibrated in 1X PBS ...
-
No products found
because this supplier's products are not listed.
Yingyan Zhou, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
C1-INH protein levels were measured using the SPAplus automated protein analyzer (The Binding Site Group Ltd, Birmingham, UK) – which utilizes turbidimetric measurement to determine protein level – according to manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Valérie Pinarello, et al.,
bioRxiv - Microbiology 2022
Quote:
... expression of protein was induced in 45 mL BHI medium supplemented with bleomycin and tetracyclin 10 mg/mL (Jena Bioscience). 5 ml of culture from nonclonal selection was added incubated for 72 h at 26°C with shaking at 100 rpm ...
-
No products found
because this supplier's products are not listed.
Anna L. Tyler, et al.,
bioRxiv - Genomics 2022
Quote:
... or a high-fat, high-sucrose (HF/HS) diet (45% fat, 40% carbohydrates, and 15% protein) (Envigo Teklad TD.08811, Envigo, Madison, WI). Mice were maintained on this diet for 26 weeks.
-
No products found
because this supplier's products are not listed.
Albrecht Fröhlich, et al.,
bioRxiv - Neuroscience 2022
Quote:
... RNA was extracted using the ISOLATE II RNA/DNA/Protein kit (Bioline, Germany) following the manufacturer’s instructions ...
-
No products found
because this supplier's products are not listed.
Aisha A. AlJanahi, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... RNA-protein complexes (RNPs) were prepared by mixing Cas9 protein 30-60 ug (PNA Bio) and 15-30 ug of gRNA per aliquot followed by incubation of 10 minutes at room temperature ...
-
No products found
because this supplier's products are not listed.
Tiffany A. Fillier, et al.,
bioRxiv - Neuroscience 2021
Quote:
... 45-50 day old Long-Evans rats from Charles River Laboratories (Canada ...
-
No products found
because this supplier's products are not listed.
Neil P. Blackledge, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Antibody-bound nucleosomes were captured using protein A agarose (Repligen) beads ...