Labshake search
Citations for Diagenode :
1 - 50 of 302 citations for RNA Binding Motif Protein 45 RBM45 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2020Quote: ... or 15 µg of chromatin and 2.5 µg of H3K27ac and H3K9me3 antibody (Active Motif #3913, Diagenode #C15410193). Final DNA purification was performed using the GeneJET PCR Purification Kit (Thermo Scientific ...
-
bioRxiv - Developmental Biology 2019Quote: ... The lysate was sonicated on ice at 30% amplitude for 3 minutes (15 seconds on 45 seconds off) followed by 40% amplitude for 30 seconds (15 seconds on 45 seconds off) in a Bioruptor (Diagenode). The sonicated lysate was supplemented with 1% NP-40 alternative and incubated rotating for 1 hour at 4C ...
-
bioRxiv - Developmental Biology 2020Quote: ... All ChIPs were incubated overnight with the respective antibodies (CTCF Active Motif 61311; H3K27me3 Millipore 17-662; H3K27ac Diagenode C15410196; RAD21 abcam ab992) and precipitated after a 2h long incubation with magnetic beads (Dynabeads Protein A ...
-
bioRxiv - Cancer Biology 2021Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
-
bioRxiv - Cancer Biology 2021Quote: ... The DNA-protein-antibody complexes were captured by DiaMag protein A-coated magnetic beads (Diagenode; Cat#: C03010020) by incubating at 4 °C for 2 hours ...
-
bioRxiv - Molecular Biology 2023Quote: ... Antibody-bound chromatin was then coupled to protein A beads (Diagenode, C03020002) for three hours at 4° C ...
-
bioRxiv - Immunology 2020Quote: ... and DNA sheared by sonication (45 cycles; 30 s on/off; Bioruptor Pico, Diagenode) in aliquots (0.2 ml) ...
-
bioRxiv - Genomics 2021Quote: ... Primary antibodies against the following proteins were applied: H3K4me1 (1:100, C15410037, Diagenode), H3K27ac (1:100 ...
-
bioRxiv - Genomics 2021Quote: ... Binding was stopped by adding 57 µL 2 M glycine (Diagenode, cat. C01011000) and incubating the sample for 5 min at room temperature ...
-
bioRxiv - Microbiology 2021Quote: ... and sonicated for 45 min (intervals: 20 s on, 40 s off) using a Bioruptor (Diagenode). After clearing the lysates ...
-
bioRxiv - Molecular Biology 2020Quote: ... 1 µg of mouse anti-RNA Polymerase II CTD monoclonal antibody (CMA601)35 or Monoclonal Pol II Antibody – ChIP-seq grade (Diagenode) for RNAPII ...
-
bioRxiv - Developmental Biology 2023Quote: ... Immunolabeling of Cas9 protein by monoclonal anti-mouse Cas9 antibody at 1:500 (4G10; Diagenode) was blocked in PBS Super Block (37580 ...
-
bioRxiv - Genetics 2022Quote: ... The antibodies used were mouse anti-RNA Pol II (Diagenode C15100055-100 RRID: AB_2750842) and goat anti-KLF1 (Origene TA305808) ...
-
bioRxiv - Microbiology 2023Quote: ... the different antibodies were previously incubated at 4°C with protein G-coated magnetic beads (DiaMag, Diagenode), protease inhibitor cocktail and 0.1% BSA ...
-
bioRxiv - Cell Biology 2023Quote: ... Chromatin was fragmented by sonication at 4°C using a Bioruptor 300 (Diagenode; 45 cycles, 30s on/30s off). Immunoprecipitation used 4 μg ChIP validated antibody against NF-κB p65 (Millipore ...
-
bioRxiv - Developmental Biology 2023Quote: ... Chromatin preparation continued as per Active Motif protocol except for the sonication steps which were performed in a Bioruptor® Plus sonication device by Diagenode with the following settings ...
-
bioRxiv - Molecular Biology 2019Quote: ... first with a single 30s pulse with a probe sonicator (labtech Soniprep 150) on ice followed by a further 45 cycles using a cooled Bioruptor (Diagenode; 1 min cycles of 30sec on / 30 sec off on ‘high’ setting at 4 °C) ...
-
bioRxiv - Genomics 2022Quote: ... DNase I treatment was replaced by a step of chromatin shredding by sonication in an ice-cold bath (15 min, High, 45 sec ON/OFF, Diagenode). The GelFree fractionation was omitted to avoid loss of material because the eluate after the second purification step was judged sufficiently pure.
-
bioRxiv - Microbiology 2022Quote: ... and incubation on ice for 30 min followed by sonication for 45 min (30 s on, 30 s off) in a Bioruptor Pico (Diagenode) at 4°C ...
-
bioRxiv - Developmental Biology 2023Quote: ... these were then lysed in 1% SDS buffer and the chromatin was sheared into 300-1000 bp fragments by sonicating for 40 cycles (30 sec on and 45 sec off) using a bioruptor (Diagenode). Immunoprecipitation (IP ...
-
bioRxiv - Molecular Biology 2021Quote: ... Fixed cells were resuspended in 1 ml RIPA Binding Buffer and placed in the Bioruptor Pico (B01060010, Diagenode) for 10 cycles of 30 seconds on ...
-
bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196 ...
-
bioRxiv - Microbiology 2023Quote: ... 10 μg total RNA was incubated overnight at 4 °C with Dynabeads™ Protein G magnetic beads pre-bound with anti-Ψ (Diagenode) according to manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2022Quote: ... RNA was then incubated at 4°C overnight with or without 12.5 μg of anti-5hmC antibody (C15220001, Diagenode) in freshly prepared 1X Immunoprecipitation (IP ...
-
bioRxiv - Cancer Biology 2020Quote: ... samples were incubated by rotating overnight at 4°C with primary antibody or irrelevant IgGs and then incubated with protein-A agarose beads (Diagenode; C03020002) previously blocked with 0.5% BSA for 3 hr at 4 °C with rotation ...
-
bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196; H3K4me3, Diagenode 3C15410003). Reverse-crossed IP sample were pulled down with streptavidin C1 beads (Life Technologies) ...
-
bioRxiv - Neuroscience 2021Quote: ... equal volume of sheared chromatin was incubated overnight with 3 μg of antibody in iC1b buffer with protease inhibitors and BSA and washed DiaMag Protein A-coated magnetic beads (Diagenode Cat# C03010020-220) on a tube rotator at 4°C overnight ...
-
bioRxiv - Neuroscience 2023Quote: ... RNA samples were incubated overnight at 4°C with 12.5 µg of anti-5-hmC antibody (Diagenode rat monoclonal MAb-633HMC) or without antibody as negative control in IP buffer (750 mM NaCl ...
-
bioRxiv - Cancer Biology 2023Quote: ... incubated in RNA Extracol with RNA extraction beads (Diagenode), sonicated using Bioruptor Plus (Diagenode) ...
-
bioRxiv - Genomics 2021Quote: ... Dynal Protein A magnetic beads (Diagenode) and ChIP-graded anti-myc antibody (ab9132 ...
-
bioRxiv - Biochemistry 2023Quote: ... Unblocked protein A beads (Diagenode, C03020003) were used to pulldown the antibody-protein complexes ...
-
bioRxiv - Cancer Biology 2022Quote: ... RNA extraction beads (Diagenode) in a Diagenode Bioruptor Plus sonicator ...
-
bioRxiv - Neuroscience 2023Quote: ... and 20 ng of BH RNAs were used for small RNA library construction by the D-Plex Small RNA-seq Kit (Diagenode C05030001). D-Plex Single Indexes for Illumina - Set A (DiagenodeC05030010 ...
-
bioRxiv - Neuroscience 2023Quote: ... and protein extraction beads (Diagenode, NJ, USA) according to the manufacturer instructions ...
-
bioRxiv - Genomics 2023Quote: ... The RNA-seq experiment has been conducted by RNA-seq services (Diagenode Cat# G02030000). RNAs have been firstly quantified using Qubit™ RNA HS Assay Kit RNA HS Assay Kit (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2021Quote: ... RNA Polymerase II (Diagenode, C15100055), RNA Polymerase II (gift from H ...
-
bioRxiv - Genetics 2023Quote: ... H3K27ac antibody (Diagenode) and isotype control IgG (provided in the kit ...
-
bioRxiv - Genetics 2023Quote: ... H3K4me1 antibody (Diagenode), H3K27ac antibody (Diagenode ...
-
bioRxiv - Immunology 2019Quote: ... Poly(A)+ RNA-seq libraries were constructed using CATS RNA-seq kit v2 (Diagenode, C05010041). Pooled libraries were sequenced on an Illumina HiSeq 2500 in rapid-run mode with paired-end reads.
-
bioRxiv - Neuroscience 2022Quote: ... Chromatin-protein complexes were immunoprecipitated with protein A/G magnetic beads and washed sequentially according to the manufacturer (Diagenode, C01010055). DNA fragments were then purified using IPure beads v2 (Diagenode ...
-
bioRxiv - Developmental Biology 2019Quote: ... overnight and Protein A magnetic beads (Diagenode C03010020) the following day for 2 hours ...
-
bioRxiv - Cancer Biology 2022Quote: ... homogenization was performed using protein extraction beads (Diagenode) and sonication in the lysis buffer followed by centrifugation to remove solid remains (Diagenode Bioruptor Plus sonicator manufacturer’s protocol for protein extraction from tissue fragments) ...
-
bioRxiv - Developmental Biology 2019Quote: ... ChIP was performed using Protein G beads (Diagenode) O/N using a H3K27ac antibody (Active Motif ...
-
bioRxiv - Microbiology 2023Quote: ... magnetic beads coated with DiaMag protein A (Diagenode), and antibodies against epitope tags (HA or MYC ...
-
bioRxiv - Genomics 2023Quote: ... RNA-seq was done by Diagenode Inc ...
-
bioRxiv - Cancer Biology 2019Quote: ... Antibodies used for this study included: 5mC-33D3 monoclonal antibody (Diagenode C15200081), 5hmC antibody (developed in-house by EDL) ...
-
bioRxiv - Molecular Biology 2021Quote: ... H3 antibody (Diagenode C15200011), HAtag antibody (abcam 9110) ...
-
bioRxiv - Plant Biology 2021Quote: ... Protein extract was sonicated using a cooled Biorupter (Diagenode) for 10 minutes using high power with 30 seconds on 30 seconds off cycle ...
-
bioRxiv - Plant Biology 2020Quote: ... magnetic beads (DiaMag protein A-coated magnetic beads: Diagenode) were incubated with each antibody overnight ...
-
bioRxiv - Molecular Biology 2023Quote: ... Total proteins were sonicated on a Bioruptor Plus (Diagenode) (position high ...