Labshake search
Citations for NanoTemper :
1 - 50 of 202 citations for RNA Binding Motif Protein 45 RBM45 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2019Quote: Solution protein-protein binding was monitored by microscale thermophoresis (MST) with a Monolith NT.115 (Nanotemper). The target proteins were portions of the CsoS2 NTD fused to Superfolder GFP and used at a concentration of 50 nM ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... The protein binding was measured by the microscale thermophoresis technique on a Monolith NT.115 instrument (Nanotemper). Protein (10 nM ...
-
bioRxiv - Plant Biology 2022Quote: ... Binding data were analyzed using MO.AFFINITY ANALYSIS software (NanoTemper Technologies).
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... Binding assays were performed using NanoTemper NT.LabelFree instrument (NanoTemper Technologies). All experiments were carried out at room temperature in hydrophobic capillaries with 20% light-emitting diode power (fluorescence lamp intensity ...
-
bioRxiv - Molecular Biology 2023Quote: The affinity between RNA and protein and between proteins was measured by MST on a Monolith NT.115 (NanoTemper, Germany). Replicates represent individual measurements performed on different days using proteins from the same prep ...
-
bioRxiv - Cell Biology 2021Quote: All binding data were analyzed using MO.Control and MO.Affinity software (NanoTemper).
-
bioRxiv - Biochemistry 2022Quote: ... Binding to EmrE3 was measured using microscale thermophoresis (Nanotemper, Munich, Germany). For these experiments ...
-
bioRxiv - Plant Biology 2022Quote: ... Binding was detected by a Monolith NT.115 instrument (NanoTemper Technologies) at 24 °C with 80 % excitation power and 40 % MST power ...
-
bioRxiv - Biochemistry 2022Quote: DNA-binding affinity was measured using a Monolith NT.115 (NanoTemper Technologies). The XPA samples were buffer exchanged into buffer containing 50 mM Tris-HCl (pH 7.8) ...
-
bioRxiv - Biochemistry 2020Quote: Binding affinities were measured using the Monolith NT.115 (NanoTemper Technologies, GmbH). Proteins were fluorescently labelled with Atto488 according to the manufacturer’s protocol ...
-
Receptor tyrosine kinases regulate signal transduction through a liquid–liquid phase separated statebioRxiv - Cell Biology 2019Quote: The binding affinities were measured using the Monolith NT.115 (NanoTemper Technologies, GmbH). Proteins were fluorescently labelled with Atto488 according to the manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2020Quote: DNA binding reactions were loaded into Monolith NT.115 Premium Capillaries (NanoTemper Technologies) and the thermophoretic response was monitored with 30 % infrared (IR ...
-
bioRxiv - Biophysics 2020Quote: Binding experiments were performed using a Monolith NT.115 Microscale Thermophoresis (MST) instrument (NanoTemper) at room temperature in phosphate buffer saline with 0.1% Tween-20 (PBS-T 0.1%) ...
-
bioRxiv - Cancer Biology 2019Quote: ... Binding measurements were performed on a Monolith NT.115 Blue/Red instrument (Nanotemper GmbH) at 40 % LED power and 40 % MST power ...
-
bioRxiv - Biochemistry 2022Quote: ... Binding curves were fitted and affinities (Kd) were calculated using MO.Affinity Analysis software (NanoTemper).
-
bioRxiv - Cell Biology 2021Quote: ... The RNA-protein mixtures were analyzed in premium capillaries using the Monolith NT.115T MST (NanoTemper Technologies). Measurements were carried out at 25 °C ...
-
bioRxiv - Biochemistry 2022Quote: Binding affinity analysis by microscale thermophoresis was performed using the Monolith NT instrument (Nanotemper Technologies). All reactions (in triplicates ...
-
bioRxiv - Cancer Biology 2023Quote: Binding affinities were estimated by microscale thermophoresis using a Monolith NT.115 device (NanoTemper, Germany). We measured the thermophoretic movements of labeled molecules within a temperature gradient ...
-
bioRxiv - Biochemistry 2020Quote: ... the binding affinity between SpaKC and SpaAleader was measured using Monolith NT.115 Pico (Nanotemper Technologies). SpaKC was fluorescently labelled by the labeling kit (Monolith Protein Labeling Kit RED-NHS 2nd Generation (Amine Reactive) ...
-
bioRxiv - Plant Biology 2022Quote: Binding experiments were performed by microscale thermophoresis with a Monolith NT.115 (NanoTemper Technologies, Munich, Germany). MBP fusions to the regulatory loop of IRT1 or IRT14HA were labelled with the Monolith NT™ Protein Labeling Kit RED according to the instructions provided by the manufacturer ...
-
bioRxiv - Plant Biology 2022Quote: ... cAMP binding assays to G-actin were performed with the Monolith NT.115 (Nanotemper, Munich, Germany) using 50 nM NTred-maleimid 2nd generation labelled G-actin and increasing concentrations of cAMP ...
-
bioRxiv - Molecular Biology 2022Quote: Binding assays were performed by electrophoretic mobility shift assay (EMSA) and Microscale thermophoresis (MST, NanoTemper Munich). Dilution series (1.5 fold ...
-
bioRxiv - Molecular Biology 2023Quote: Binding affinity comparisons via microscale thermophoresis were performed using the Monolith NT.115 instrument (NanoTemper Technologies). The CTD polypeptides (CTD ...
-
bioRxiv - Biochemistry 2023Quote: ... binding affinities were determined via microscale thermophoresis on a Monolith NT.115 instrument (NanoTemper Technologies, Germany). Briefly ...
-
bioRxiv - Molecular Biology 2024Quote: Binding affinity comparisons via microscale thermophoresis were performed using the Monolith NT.115 instrument (NanoTemper Technologies). The CTD polypeptides (CTD ...
-
bioRxiv - Cancer Biology 2019Quote: ... Fluorescence values from the binding reactions were determined using the Monolith NT.115 (NanoTemper Technologies, München, Germany). Binding data was analyzed using GraphPad Prism (GraphPad Software ...
-
bioRxiv - Biochemistry 2022Quote: ... The binding affinity measurements were carried out using the modified manufacturer’s protocol (Monolith NT.115pico, NanoTemper Technologies) in the Center for Macromolecular Interactions in Harvard Medical School (Boston ...
-
bioRxiv - Molecular Biology 2023Quote: ... Each binding experiment was performed in three independent runs and resulting MST data loaded to MO.Analysis (NanoTemper) software to estimate the Kd for MRE11 dimerization ...
-
bioRxiv - Biochemistry 2022Quote: ... and contained constant concentration of 45 nM RED-NHS labelled Mer3 (labelling was performed according to the manufacturer’s protocol - Nanotemper Technologies) and increasing concentrations of Top3/Rmi1 ...
-
bioRxiv - Molecular Biology 2022Quote: The kinetic measurements of PfCoSP-gDNA binding were conducted using Monolith NT.115 instrument (NanoTemper Technologies, Munich, Germany). 10 μM PfCoSP was labeled using NanoTemper’s Protein Labeling Kit RED-NHS (L001 ...
-
bioRxiv - Biochemistry 2023Quote: Binding experiments were performed using microscale thermophoresis (MST).39 Experiments were performed on a Monolith NT.115 (NanoTemper) using 20% light-emitting diode power ...
-
bioRxiv - Biophysics 2021Quote: Thermal stability and binding affinity were determined using nanoscale differential scanning fluorimetry on a Prometheus NT.48 (NanoTemper Technologies). Capillaries were filled with 10 μL of respective samples in duplicates and loaded into the reading chamber ...
-
bioRxiv - Microbiology 2023Quote: Nucleotide binding assays based on microscale thermophoresis were performed with a Monolith NT.115 instrument (NanoTemper Technologies GmbH, Germany) using Monolith NT Premium Capillaries ...
-
bioRxiv - Biochemistry 2020Quote: The binding affinities of compound 20 to Sc-Dyn-lysoMut was measured using Monolith NT.115 (NanoTemper Technologies, Munich, Germany). Sc-Dyn-lysoMut was labeled with the Monolith NT Protein Labeling Kit RED-NHS ...
-
bioRxiv - Biophysics 2020Quote: The binding affinity of spike to Sb23 was measured by microscale thermophoresis using the MST NT.115 device (NanoTemper Technologies). Sb23 was labelled via the His6-tag with RED-tris-NTA dye (NanoTemper Technologies ...
-
bioRxiv - Molecular Biology 2021Quote: ... Signal to noise ratio was used to evaluate the quality of the binding data according to NanoPedia instructions (NanoTemper Technologies). Only a signal-to-noise ratio of more than 5 was considered acceptable while a signal to noise of more than 12 was considered excellent.
-
bioRxiv - Cell Biology 2021Quote: Binding of cevipabulin to the seventh site was detected with an MST assay with a Monolith NT.115 instrument (NanoTemper Technologies). Purified tubulin was labeled using the Monolith protein labeling kit RED-NHS (NanoTemper Technologies ...
-
bioRxiv - Biochemistry 2023Quote: ... The best data quality was obtained at 90% MST power which was thus used for quantifying the binding affinities using the MO.Affinity Analysis software (NanoTemper Technologies, Germany).
-
bioRxiv - Biochemistry 2023Quote: Microscale thermophoresis (MST) analysis was carried out to determine the binding affinity between hexon and hLF using Monolith NT.115 (Nanotemper Technologies). Hexon was labelled with AlexaFluor594 (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: Proteins were labeled using the Monolith NT Protein Labeling Kit RED (NanoTemper Technologies) according to the manufacturer’s protocol and using a buffer consisting of 20 mM HEPES ...
-
bioRxiv - Molecular Biology 2023Quote: MST experiments to measure the binding affinities between TadAs and the 19-nt DNA substrate were performed on a Monolith NT.115 system (NanoTemper Technologies, Germany) using the nano BLUE detector ...
-
bioRxiv - Immunology 2019Quote: ... ILT7 purified protein was labeled using the Protein Labeling Kit RED-NHS (NanoTemper Technologies). The labeling reaction was performed according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... EMCV 2A protein was labelled using the Protein Labelling Kit RED-Maleimide (NanoTemper Technologies) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: Protein-protein interactions were monitored by MST using a Monolith NT.115 instrument (Nanotemper Technologies). His6-MyoVa GTD was labeled using RED-NHS 2nd Generation (Amine Reactive ...
-
bioRxiv - Cell Biology 2020Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein labeling kit (Nanotemper Technologies). Labelled protein was added to a serial dilution of unlabeled DAG ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were labeled using the RED-tris-NTA His tag protein-labeling kit (Nanotemper Technologies). Labeled proteins were added to a serial dilution of unlabeled DAG or liposomes prepared in binding buffer (20 mM Tris ...
-
bioRxiv - Cancer Biology 2023Quote: ... EED and JMJD3 proteins were labeled with Protein Labeling kit RED-NHS (NanoTemper, MO-L001). At least three independent experiments were performed for each binding reaction.
-
Long-Range Electrostatic Interactions Significantly Modulate the Affinity of Dynein for MicrotubulesbioRxiv - Biophysics 2021Quote: We measured the binding affinity of MTBDs (fluorescently labeled target) to microtubules (unlabeled ligand) using a Monolith NT.115 MST system (NanoTemper Technologies, GmbH, Munich, Germany). We labeled the MTBDs with a 2nd Generation RED-tris-NTA His-Tag Labeling Kit (MO-L018 ...
-
bioRxiv - Biochemistry 2022Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (Nanotemper Technologies). His LRRK2 Armadillo (1-552) ...
-
bioRxiv - Biochemistry 2023Quote: Protein–protein interactions were monitored by microscale thermophoresis using a Monolith NT.115 instrument (NanoTemper Technologies). His LRRK2 Armadillo (1–552 ...