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No products found
because this supplier's products are not listed.
Arash Khorrami Jahromi, et al.,
bioRxiv - Bioengineering 2023
Quote:
... human coronavirus (HCoV-229E) spike protein (RDC3141; Cedarlane), Middle East respiratory syndrome coronavirus (MERS-CoV ...
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No products found
because this supplier's products are not listed.
Asheley P. Chapman, et al.,
bioRxiv - Immunology 2020
Quote:
... His-S1 or ecto-spike proteins were plated on half-area high-binding 96-well polystyrene plates (Corning) at 1 μg/mL or 0.5 μg/mL ...
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No products found
because this supplier's products are not listed.
Bernard Vanhove, et al.,
bioRxiv - Immunology 2022
Quote:
... recombinant SARS-CoV-2 Spike S1-HIS proteins (Sino Biological Europe for the Wild Type Wuhan, or Omicron from Sino Biological Europe and from Covalab, France ...
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No products found
because this supplier's products are not listed.
Jing Zhao, et al.,
bioRxiv - Plant Biology 2023
Quote:
... His-tag antibody (Tiangen, Beijing, China) and GST antibody (Tiangen).
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No products found
because this supplier's products are not listed.
Karla M. Márquez-Nogueras, et al.,
bioRxiv - Physiology 2023
Quote:
... were crossed with αMHC Mer-Cre-Mer (Jackson laboratories) to obtain gCaMP6F-tdTomato-cardiomyocyte specific mice under tamoxifen induction ...
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No products found
because this supplier's products are not listed.
Sakshi Gera, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... LPS-B5 (Lipopolysaccharide E. coli 055:B5, Calbiochem, Catalog # 437625,), LPS-B4 (LPS-EB ...
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No products found
because this supplier's products are not listed.
Alexandra Atalis, et al.,
bioRxiv - Immunology 2022
Quote:
Adjuvanted PLGA-PEI NPs and the S1 subunit of the SARS-CoV-2 spike protein (Novus Biologicals, Cat# NBP2-90985 ...
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No products found
because this supplier's products are not listed.
Wander Van Breedam, et al.,
bioRxiv - Bioengineering 2021
Quote:
... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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No products found
because this supplier's products are not listed.
Matthew J. Burke, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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No products found
because this supplier's products are not listed.
Rayan Khaddaj-Mallat, et al.,
bioRxiv - Neuroscience 2021
Quote:
... The levels of S protein in different brain regions upon its intranasal infusion were measured using His-Tag protein ELISA kit that allows detection and quantification of poly-histidine-tagged proteins in a biological sample (Cell Biolabs Inc., CA, USA). The experimental procedure was performed as recommended by the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Feng Li, et al.,
bioRxiv - Genetics 2019
Quote:
A Hi-C library was constructed with the ProxiMeta Hi-C kit from Phase Genomics v 1.0 containing the enzyme Sau3A ...
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No products found
because this supplier's products are not listed.
Matthieu P.M.H. Benoit, et al.,
bioRxiv - Biochemistry 2023
Quote:
... the SNAPf-EGFP-6His tag was replaced with a strep-II tag (IBA Lifesciences GmbH) using Q5 mutagenesis (New England Biolabs Inc. ...
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No products found
because this supplier's products are not listed.
Natacha S. Ogando, et al.,
bioRxiv - Microbiology 2020
Quote:
... MERS-CoV nsp14 mutant expression plasmids were generated by Quikchange site-directed mutagenesis using Accuzyme DNA polymerase (Bioline) following the manufacturer’s instructions ...
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No products found
because this supplier's products are not listed.
Andre Watson, et al.,
bioRxiv - Bioengineering 2020
Quote:
... A neutralizing monoclonal IgG antibody against the SARS-CoV-2 spike glycoprotein (CR3022, antibodies-online), ACE2 (Sino Biological) ...
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No products found
because this supplier's products are not listed.
Aziz Gauhar, et al.,
bioRxiv - Bioengineering 2021
Quote:
... S1 protein was biotinylated at the AVI-tag using the BirA biotin-protein ligase kit (Avidity Biosciences) and premixed at 5 nM with serial dilutions of VNAR-hFc antibodies for 1 hr at 4°C ...
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No products found
because this supplier's products are not listed.
Camilla Tiezzi, et al.,
bioRxiv - Immunology 2022
Quote:
... p8.74 packaging vector, pseudotyping vector coding for Spike glycoproteins (Wuhan-Hu-1; B.1 Lineage, China) and pREV with PEI (Polysciences, Inc., Warrington, PA, USA) (1 mg/mL in PBS ...
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No products found
because this supplier's products are not listed.
James B. Winans, et al.,
bioRxiv - Microbiology 2022
Quote:
... was stained with Anti-6X His Epitope Tag (Rabbit) antibody conjugated to Dylight 405 (Rockland Immunochemicals, cat. # 600-446-382) added to the media at a concentration of .1μg/mL for the entirety of the experiment ...
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No products found
because this supplier's products are not listed.
A. Green, et al.,
bioRxiv - Microbiology 2020
Quote:
... Human Coronavirus Strain HCoV-229E (#VR-740) was sourced from ATCC (American Type Culture Collection) and high titer viral stocks were propagated and maintained by BioScience Laboratories Inc (Bozeman ...
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No products found
because this supplier's products are not listed.
Vilde Leipart, et al.,
bioRxiv - Biochemistry 2022
Quote:
... We made three sets of blank samples: buffer blank and two sets with SUMO-1 (human, His-tag, Enzo Life Sciences). One collection of SUMO-1 samples were incubated with 25uM ZnCl2 at 4 °C for 1 hour ...
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No products found
because this supplier's products are not listed.
Carolin Fritz, et al.,
bioRxiv - Cell Biology 2024
Quote:
... His (MP Biomedicals) plates ...
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No products found
because this supplier's products are not listed.
Yu-Feng Chien, et al.,
bioRxiv - Bioengineering 2020
Quote:
... a TAG lens (TAG lens 2.5β, TAG Optics) was placed directly on top of an objective (UPLFLN 10XP, Olympus) to enable high-speed axial scan of the focus ...
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No products found
because this supplier's products are not listed.
Pieter Nibbering, et al.,
bioRxiv - Plant Biology 2020
Quote:
... 18µl α-Fucosidase (E-FUCTM) and 20µl α-Arabinofuranosidase (E-ABFAN) from Megazyme in a 50 mM sodium acetate buffer pH5 at 37 °C for 4 hours (again ...
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No products found
because this supplier's products are not listed.
Yusong Guo, et al.,
bioRxiv - Biochemistry 2021
Quote:
... and STAMBPL1 (Boston Biochem #E-551). Reactions were measured with similar conditions as were used for measuring STAMBPJAMM inhibition ...
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No products found
because this supplier's products are not listed.
Seungyoon B. Yu, et al.,
bioRxiv - Neuroscience 2023
Quote:
... coverslips were transferred to Hibernate E (BrainBits) medium containing 5nM TMRM as described previously 60 ...
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MERS-CoV, Recombinant Protein, Subunit 1 of Spike Protein [His].
Cat# VReP-Wyb113,
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Xiaotian Tan, et al.,
bioRxiv - Bioengineering 2020
Quote:
... The human-cell-expressed SARS-CoV Spike S1-His recombinant protein was purchased from Creative Biolabs (VAng-Wyb7337). The recombinant CR3022 therapeutic antibody was purchased from Creative Biolabs (MRO-1214LC) ...
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No products found
because this supplier's products are not listed.
Bennett J. Davenport, et al.,
bioRxiv - Immunology 2021
Quote:
... or mouse anti-MERS-CoV spike antibody clone D12 (Absolute Antibody, Ab00696) prepared in a 2-fold dilution series in PBS-T with a starting dilution of 1:200 and signal was developed as described above.
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No products found
because this supplier's products are not listed.
Huan Wang, et al.,
bioRxiv - Biochemistry 2021
Quote:
... anti-His tag (Solarbio Life Sciences, China, K200060M), anti-toll-like receptor-4 (TLR-4 ...
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No products found
because this supplier's products are not listed.
P.D. Kirkland, M.J. Frost,
bioRxiv - Microbiology 2020
Quote:
... The probes for the coronavirus assays (E/IP4) were both labelled with a FAM reporter and black hole quencher 1 (Biosearch Technologies) and the XIPC probe with a Vic reporter and TAMRA quencher (Life Technologies ...
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No products found
because this supplier's products are not listed.
Annette Choi, et al.,
bioRxiv - Microbiology 2023
Quote:
... we designed and ordered linear peptides that mimic the S1/S2 domain of the S glycoprotein for selected coronaviruses (Biomatik, Kitchener, Ontario). Information about the series of amino acid residues within the S1/S2 domain were obtained from NCBI ...
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No products found
because this supplier's products are not listed.
Alina Remeeva, et al.,
bioRxiv - Biophysics 2021
Quote:
... with the mutation C85A and C-terminal 6×His tag) was obtained as a synthetic gene from Evrogen (Russia) and introduced into the pET11 expression vector (Novagen ...
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No products found
because this supplier's products are not listed.
Hayden T. Pacl, et al.,
bioRxiv - Microbiology 2021
Quote:
... was treated with 300 nM of purified recombinant SARS-Cov2 NSP13 with an N-terminal His-tag (Cayman Chemicals #30589) in the presence of 50 mM Tris pH-7.4 ...
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No products found
because this supplier's products are not listed.
Lydia M. Castelli, et al.,
bioRxiv - Systems Biology 2021
Quote:
... compound E 0.1 µM (Cpd E; Tocris), BDNF 10ng/mL (ThermoFisher Scientific) ...
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No products found
because this supplier's products are not listed.
MS Dyer, et al.,
bioRxiv - Neuroscience 2020
Quote:
... spike threshold in Spike 2 v7.10 (Cambridge Electronic Design) and action potential properties in pCLAMP (Molecular Devices). A total of 20 mice were used for intrinsic excitability experiments.
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No products found
because this supplier's products are not listed.
Jamin Liu, et al.,
bioRxiv - Microbiology 2022
Quote:
... 293T cells were transfected with arenavirus glycoprotein expression plasmids using TransIT-LT1 (Mirus Bio). Cells were transduced the following day with VSV-ΔG-GFP (Kerafast)(45 ...
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No products found
because this supplier's products are not listed.
Laura Fumagalli, et al.,
bioRxiv - Neuroscience 2019
Quote:
... and an S1 Environmental System incubation chamber (Carl Zeiss) maintained at 37 °C and 5% CO2 ...
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No products found
because this supplier's products are not listed.
Kirsi Savijoki, et al.,
bioRxiv - Microbiology 2021
Quote:
... Rifampicin solution (TOKU-E) in water at a final concentration of 400 µg mL-1 corresponding to 64 x MIC (minimum inhibitory concentration ...
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No products found
because this supplier's products are not listed.
Kari Martyniak, et al.,
bioRxiv - Bioengineering 2022
Quote:
... coli (GCI-L3; Genecopoeia) using a commercial kit (ZymoPURE II Plasmid Maxiprep Kit ...
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No products found
because this supplier's products are not listed.
Till Stephan, et al.,
bioRxiv - Cell Biology 2023
Quote:
... Spot tag (ChromoTek, Planegg-Martinsried, Germany), TOMM22 (Merck ...
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No products found
because this supplier's products are not listed.
Violaine Tribollet, et al.,
bioRxiv - Cell Biology 2020
Quote:
E-plates 16 PET (ACEA Biosciences) were coated with 1.5 µg/cm2 collagen I (Corning ...
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No products found
because this supplier's products are not listed.
Anna Onnis, et al.,
bioRxiv - Immunology 2022
Quote:
... and E (SEE; Toxin Technologies, #ET404) for 2 h and labelled with 10 μM Cell Tracker Blue for the last 15 min of the incubation with the superantigens (+ SAgs) ...
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No products found
because this supplier's products are not listed.
Anthony N. Cutrupi, et al.,
bioRxiv - Neuroscience 2022
Quote:
... 0.1 µM Compound-E (StemCell Technologies), 2ng/mL BDNF ...
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No products found
because this supplier's products are not listed.
Prashant P. Damke, et al.,
bioRxiv - Microbiology 2022
Quote:
... using a microplate reader (BioTek Synergy HI) to record the absorbance at 650 nm ...
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No products found
because this supplier's products are not listed.
Bharti Bhatia, et al.,
bioRxiv - Microbiology 2021
Quote:
... and a FluorChem E system (Protein simple).
-
No products found
because this supplier's products are not listed.
Ana Zúñiga, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... into 500μL of Azure Hi-Def medium (Teknova, 3H5000) supplemented with 0.4% of glycerol ...
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No products found
because this supplier's products are not listed.
Michael G. Spelios, et al.,
bioRxiv - Immunology 2021
Quote:
... and His-tagged SARS-CoV-2 S1 RBD protein lacking the S1/S2 boundary furin site (EpiGentek) were added at a concentration of 10 ng/well to the Ni-NTA-coated strips and incubated for 1h at 37°C ...
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No products found
because this supplier's products are not listed.
John N. Koberstein, et al.,
bioRxiv - Biochemistry 2020
Quote:
... coli MBP (malE) was obtained as a gBlock (IDT DNA, Table S1). BsaI sites were added to ends by PCR with complementary overhangs for golden gate cloning into pATT-Dest (Addgene plasmid #79770 ...
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No products found
because this supplier's products are not listed.
Daisuke Ogasawara, et al.,
bioRxiv - Biochemistry 2024
Quote:
... N-term strep-His tag was first inserted into phCMV3 vector (Genlantis, cat # P003300) using Q5® Site-Directed Mutagenesis Kit (New England BioLabs ...
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No products found
because this supplier's products are not listed.
Tomas Kouba, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... 21-mer-sd or 21-mer-d 3′ and 14-mer 5′ ends vRNAs (IBA) were used in combination with synthetic 12-or 13-mer capped RNA (TriLink Biotechnologies) as primer (Table 2 ...
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No products found
because this supplier's products are not listed.
Saroj Khadka, et al.,
bioRxiv - Microbiology 2022
Quote:
... or 1:5,000 mouse anti-His-Tag (primary) then 1:5000 goat anti-mouse IgG(H+L)-HRP (secondary) (SouthernBiotech). Blots were developed with ECL Western Blotting Substrate (Pierce ...
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No products found
because this supplier's products are not listed.
Antonios Apostolopoulos, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... (e-Myco VALiD Mycoplasma PCR Detection Kit, iNtRON Biotechnology).