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Citations for VWR :
1 - 50 of 54 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... 6xHis-tag- and Fc-specific Dylight 800-labeled detection antibodies (6X His Tag Antibody Dylight™ 800 Conjugated (VWR) and Human IgG (H&L ...
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bioRxiv - Evolutionary Biology 2023Quote: ... containing 6-His tag and TEV cleaving site.19 In 50 mM HEPES (VWR, PA, USA), pH 7.5 including 50 mM potassium sulfate (Honeywell ...
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bioRxiv - Immunology 2022Quote: ... gentamycin (10 μg/ml for plasmid maintenance in E. coli; 100 μg/ml for plasmid maintenance in P. aeruginosa, VWR, Cat. No. 97062-974).
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bioRxiv - Genetics 2021Quote: ... anti V5-tag (VWR, MEDMMM-) and Anti-pgk1(Invitrogen ...
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bioRxiv - Biophysics 2022Quote: ... His-bind resin was purchased from VWR.
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bioRxiv - Developmental Biology 2022Quote: ... H&E routine stain was performed using Leica ST5010 Autostainer XL and Leica ST Infinity H&E Staining System kit (VWR#3801698) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% FBS (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (GIBCO ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21(DE3) (Stratagene Europe/VWR, Darmstadt, Germany). Protein expression was induced with 0.5 mM isopropyl β-d-thiogalactopyranoside for 18 h at 20 °C ...
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bioRxiv - Cell Biology 2022Quote: ... and digested with 1 mg/mL pronase E (39052, VWR) per 1 g tissue for 6-8 h at 37 °C and 5% CO2 under constant agitation ...
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bioRxiv - Plant Biology 2019Quote: 6x-His purification was conducted in 20 mL gravity columns (VWR) loaded with 4 mL HisPur™ Ni-NTA resin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... mouse anti-HIS his.h8 monoclonal (1:1000 dilution, VWR #101981-852); goat HRP-anti-rabbit and goat HRP-anti-mouse (1:1000 dilutions ...
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bioRxiv - Microbiology 2023Quote: ... coli strains were grown on Luria–Bertani agar (VWR) or in Luria–Bertani broth (VWR ...
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bioRxiv - Developmental Biology 2022Quote: ... Cryosections (10 µm thickness) were collected on His-bond glass slides (VWR) and processed following (Jacobson et al. ...
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bioRxiv - Molecular Biology 2021Quote: ... His-tagged recombinant proteins were retained on nickel magnetic beads (VWR International) and GST-tagged recombinant proteins were retained on glutathione magnetic beads (Fisher Scientific) ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 10% fetal bovine serum (Seradigm Premium Grade HI FBS, VWR), 1X penicillin-streptomycin (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... coli DH5α was grown in Luria Bertani (LB) broth (VWR), and E ...
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bioRxiv - Bioengineering 2019Quote: ... coli loaded microbeads were cultures in Lysogeny broth (LB; VWR). For the generation of degradable alginate microbeads ...
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bioRxiv - Microbiology 2021Quote: ... coli Rossetta2 (DE3) was grown in Terrific Broth (TB, VWR) for protein purification ...
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bioRxiv - Immunology 2022Quote: 3 µg/ml of spike antigens were coated onto the 384-well ELISA plates (VWR, Cat # 82051-300) overnight at 4 degree ...
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bioRxiv - Microbiology 2019Quote: ... coli BL21 (DE3) was grown in Terrific broth (VWR, PA, USA) for protein purification ...
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bioRxiv - Microbiology 2024Quote: ... Escherichia coli DH5α strains were grown in Luria-Bertani Broth (VWR) at 37°C and supplemented with 200 μg/mL hygromycin and 50 μg/mL kanamycin ...
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bioRxiv - Microbiology 2024Quote: Cells from bone marrow of 6-10-week-old male or female wild-type or Myd88-/- Ticam-/- Mavs-/- (49) C57BL/6 mice (see Table S1) were cultivated in RPMI 1640 (VWR) with 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2024Quote: ... 5 mM Na3 citrate 2H2O) with ∼75u/mL Pronase E (VWR, Radnor, PA) was added to each sample ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Tygon tubing was replaced by flexible pumping tubing (Masterflex Tygon E-3603, VWR). Outflow tubing were connected to outlet ports ...
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bioRxiv - Bioengineering 2020Quote: ... coli strains were cultured in lysogeny broth (LB) (VWR International, Randor, PA) at 37 °C with rotary shaking at 250 rpm ...
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bioRxiv - Microbiology 2019Quote: ... coli DH5α was grown in Luria Bertani (LB) broth (VWR, PA, USA), and E ...
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bioRxiv - Microbiology 2021Quote: ... coli BL21 Star (DE3) or OverExpress C43 (DE3) (Lucigen/VWR, Radnor, PA). Following induction with IPTG ...
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bioRxiv - Microbiology 2022Quote: ... coli DH5α was propagated in Luria Bertani (LB) broth (VWR, PA, USA), and E ...
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bioRxiv - Microbiology 2023Quote: ... coli were grown overnight to stationary phase in LB Lenox Broth (VWR) before cells were washed 2x with PBS and OD600 determined by NanoDrop 2000c (Thermo Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... coli DB (DBS0350636) in a type 25384-302 petri dish (VWR, Radnor, PA). 100 μg/ml dihydrostreptomycin and 100 μg/ml ampicillin were used to kill E ...
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bioRxiv - Microbiology 2022Quote: ... coli BL21 (DE3) was propagated in Terrific broth (TB) medium (VWR, PA, USA) for protein purification.
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bioRxiv - Cell Biology 2023Quote: ... coli DB (DBS0350636) in a type 2538-4302 petri dish (VWR, Radnor, PA). 100 μg/ml dihydrostreptomycin and 100 μg/ml ampicillin were used to kill E ...
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bioRxiv - Biophysics 2019Quote: ... The vial with the cell suspension was connected via Tygon S3 E-3603 tubing (VWR, inner diameter 1/32" ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 100 μg/mL of 5-florocystosine (5-FC) and grown at 24°C as listed in Supplemental Table S1 (Stork et al. 2008) (VWR, Radnor, PA, USA). A 10 mg/mL stock solution of 5-FC was prepared in distilled water and filtered sterilized ...
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bioRxiv - Bioengineering 2020Quote: All Escherichia coli strains were cultured in lysogeny broth (LB) (VWR International, Randor, PA) at 37 °C with rotary shaking at 250 rpm ...
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bioRxiv - Microbiology 2023Quote: ... coli E1 strain was grown overnight to stationary phase in LB Lenox Broth (VWR) before cells were washed 2x with PBS and OD600 determined by NanoDrop 2000c (Thermo Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... coli expression constructs were grown overnight shaking at 37 °C in Terrific Broth (VWR) supplemented with 5 mM MgSO4 and kanamycin (50μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... then treated with Pronase E (100µg/ml in Tris-HCl (pH 7.2) + 0.06% NaCl) (VWR Chemicals) for 2 hours at 60C to remove proteins covalently bound to PG ...
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bioRxiv - Cell Biology 2022Quote: ... were grown to O.D 0.3-0.6 and incubated with non-fluorescent Halo tag ligand 7-bromo-1-heptanol (7BRO, 100μM, VWR #AAH54762) for 10 min to irreversibly mask all the pre-existing ribosomal proteins ...
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bioRxiv - Plant Biology 2023Quote: ... coli DH5α and positive clones were selected via Colony PCR using Taq polymerase (VWR, Radnor, USA). Correct insertion was checked via Sanger sequencing at Eurofins Genomics (Ebersberg ...
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bioRxiv - Microbiology 2023Quote: ... coli cells were grown at 37 °C with aeration in either LB (Luria-Bertani broth; VWR) or LA (Luria Agar ...
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bioRxiv - Cell Biology 2023Quote: ... coli Gold bacteria were cultured in Luria-Bertani (LB) medium (Broth, Miller, VWR, Radnor, PA, USA) supplemented with 150 μg/ml ampicillin for 4.5 and 6 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... purification and validation of GST or His-tagged recombinant proteins from BL21(DE3) E.coli cells (VWR Cat#80030-326) were performed following vendor’s standard protocol ...
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bioRxiv - Genetics 2019Quote: ... Eluates were analysed by SDS-PAGE followed by western blotting with antibodies anti-V5 tag (VWR, MEDMMM-0168-P), anti-Pgk1 (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... 5 μL of each rescuer strain was inoculated into 500 μL of SC -his media in 96 well 2 mL deep well plate format (VWR) and shaken at 900 rpm at 30°C ...
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bioRxiv - Biochemistry 2022Quote: ... and were treated for 2hrs at 60°C with activated Pronase E (100µg/ml final concentration, pre-treated for 2hrs at 60°C in the digestion buffer; supplier: VWR). The sacculi were incubated at 100°C for 10min to inactivate the Pronase E and were washed 3 times with 1ml of MQ water ...
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bioRxiv - Biochemistry 2022Quote: ... and were treated for 2hrs at 60°C with Pronase E (100µg/ml final concentration, pre-treated for 2hrs at 60C in the digestion buffer; supplier: VWR). The sacculi were incubated at 100°C for 10min to inactivate the Pronase E ...
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bioRxiv - Microbiology 2023Quote: ... coli (E23) CRISPRi cell lines (details below) were grown overnight to stationary phase in LB Lenox Broth (VWR) before dilution 1:50 into fresh media in the morning ± 2 µM anhydrotetracycline ...