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No products found
because this supplier's products are not listed.
Liu He, et al.,
bioRxiv - Biochemistry 2022
Quote:
... coli strain BL21 (DE3) with an N-terminal (His)6-SUMO tag from a pE-SUMO-pro expression vector (LifeSensors). Cells were grown in 2YT media at 24°C to an OD600 of 0.8 and then induced with 0.1 mM isopropyl β-D-thiogalactopyranoside (IPTG ...
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No products found
because this supplier's products are not listed.
Michael P. Doyle, et al.,
bioRxiv - Immunology 2022
Quote:
... his-tagged YFV E protein (Meridian Life Science) was associated to the tips at 5 μg/mL for 60 sec ...
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No products found
because this supplier's products are not listed.
Satoshi Imanishi, et al.,
bioRxiv - Pharmacology and Toxicology 2022
Quote:
... and the corresponding His tag (R06-32BH) were obtained from SignalChem Lifesciences (Richmond ...
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No products found
because this supplier's products are not listed.
Cathal Meehan, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Recombinant IL-6 with an N-terminal his tag (Fitzgerald, Biosynth Ltd.) was immobilized on His-Pur™ Ni-NTA Resin (ThermoScientific ...
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No products found
because this supplier's products are not listed.
Jun Liu, et al.,
bioRxiv - Biochemistry 2020
Quote:
... USA. β-Galactosidase (E. coli) was purchased from Abnova (catalog #P5269). Goat anti-rabbit IgG H&L-AF488 (Catalog #ab150077 ...
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No products found
because this supplier's products are not listed.
Anil Verma, et al.,
bioRxiv - Immunology 2023
Quote:
... were labeled with biotinylated anti-His tag monoclonal antibody (ThermoScientific) and used to capture His-tagged clade C gp120 Du151 protein (Immune Technologies). The gp120-expressing beads were then incubated with triplicate 5-fold dilutions of heat-inactivated serum samples in V-bottom plates for 1h at 37°C ...
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No products found
because this supplier's products are not listed.
Livia Mazzini, et al.,
bioRxiv - Immunology 2020
Quote:
ELISA plates were coated with 1µg/mL of purified recombinant Spike S1 Protein (aa 18-676) (eEnzyme, Gaithersburg, MD, USA) or with 1µg/mL Spike-RBD (Arg319-Phe541 ...
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No products found
because this supplier's products are not listed.
Rebecca J. Hall, et al.,
bioRxiv - Microbiology 2023
Quote:
... an overnight culture was grown in 5 ml LB (E. coli MG1655 recipient) or 5 ml LB (E & O Laboratories Ltd) + 4 µg/ml cefotaxime (Alfa Aesar) (donors ...
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No products found
because this supplier's products are not listed.
Zhongyan Lu, et al.,
bioRxiv - Immunology 2021
Quote:
... IE2 and envelope glycoprotein B (Mabtech, OH) at a final concentration of 1 mg/ml for each individual peptide (Supplemental Table 2) ...
-
No products found
because this supplier's products are not listed.
Jaganathan Subramani, et al.,
bioRxiv - Microbiology 2021
Quote:
... or RBD-C tag (Dyadic International, Jupiter, FL) or different variants of spike protein B.1.1.7 (Alpha; Cube Biotech # 28718), B.1.351 (Beta ...
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No products found
Fang Wu, et al.,
bioRxiv - Biochemistry 2023
Quote:
The following primary antibodies were used for Western blotting: anti-His Tag (Applied Biological Materials, #G020; 1:4000), anti-HA Tag (Applied Biological Materials ...
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No products found
because this supplier's products are not listed.
Daniel F. Hill, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Spike Triggered Averages: Spike-triggered averages (STAs) (Dayan and Abbott, 2001 ...
-
No products found
because this supplier's products are not listed.
Samad A. Ibitokou, et al.,
bioRxiv - Immunology 2022
Quote:
... or (15 ng/mouse) of recombinant-mouse IFN-γ (rIFN-γ expressed in E. coli; PeproTech, Rocky Hill, NJ), i.p ...
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No products found
because this supplier's products are not listed.
FWN Chow, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Quantification was carried by western blot analysis in comparison to known concentrations of recombinant full length exWAGO containing an N-terminal 3XFlag-His tag using Image Studio Lite (LI-COR) software ...
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No products found
because this supplier's products are not listed.
Aleksandra Luginina, et al.,
bioRxiv - Biophysics 2023
Quote:
... The final gene construct was completed from the 3’-terminus by KpnI restriction site followed by 9×His-tag nucleotide sequence and inserted into the integrative inducible expression vector pLEXSY_I-blecherry3 (Jena Bioscience, Germany) via the BglII(5’ ...
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No products found
because this supplier's products are not listed.
Madeleine F. Jennewein, et al.,
bioRxiv - Immunology 2021
Quote:
... pαH-MERS RBD-Fc using 2 mg of polyethyleneimine (Polyscience, Cat# 24765). After 6 days of growth ...
-
No products found
because this supplier's products are not listed.
Ashok Pabbathi, et al.,
bioRxiv - Biophysics 2021
Quote:
... We incubated the washed beads with 0.25 mg/mL mouse monoclonal biotinylated anti-His Tag monoclonal antibody (AS-61250-BIOT, Anaspec Inc., Fremont, CA) for 2 hours at 22°C ...
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No products found
because this supplier's products are not listed.
Bob J. Ignacio, et al.,
bioRxiv - Cell Biology 2022
Quote:
... bacteria were collected by centrifugation (5,000 g, 3 min). E. coli B834 (a gift from S. van Kasteren, Leiden University) were incubated in SelenoMet medium (Molecular Dimensions) at 37 °C for 30 min to deplete intracellular methionine stores ...
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No products found
because this supplier's products are not listed.
Sharon R. Stevens, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Flag-tag or DDDDK-tag (MBL International Cat# M185-3L, RRID:AB_11123930); rabbit polyclonal antibodies against AnkR(Ho et al. ...
-
No products found
because this supplier's products are not listed.
Jillian M. DiMuzio, et al.,
bioRxiv - Immunology 2021
Quote:
Spike-expressing pseudovirus was generated with System Bioscience’s pPACK-SPIKE packaging system (System Biosciences, Cat # CVD19-50A-1) as per manufacturer’s protocol ...
-
No products found
because this supplier's products are not listed.
Josephine Herbst, et al.,
bioRxiv - Plant Biology 2023
Quote:
... The gRNA oligonucleotides (Table S1) were synthesized by Eurogentec. Both gRNAs were introduced into the entry vector pEn-RZ_Lb-Chimera ...
-
No products found
because this supplier's products are not listed.
Andrés López-Perrote, et al.,
bioRxiv - Biophysics 2020
Quote:
... coli cells (NZYTech) grown in LB medium ...
-
No products found
because this supplier's products are not listed.
Shuzhao Li, et al.,
bioRxiv - Biochemistry 2022
Quote:
... coli (Cambridge Isotope Laboratories ...
-
No products found
because this supplier's products are not listed.
Elisabet Bjånes, et al.,
bioRxiv - Microbiology 2024
Quote:
... coli MC1061 (MCLab). A pink ...
-
No products found
because this supplier's products are not listed.
David Swygart, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Spike trains were recorded using glass pipettes (2–3MΩ) filled with AMES solution in cell-attached configuration ...
-
Cat# HY-N6680-10 mg,
10 mg, USD $960.0
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Jie Hu, et al.,
bioRxiv - Microbiology 2020
Quote:
... and aloxistatin (E-64d; MedChemExpress) were dissolved in dimethyl sulfoxide (DMSO ...
-
No products found
because this supplier's products are not listed.
Christian W. Johnson, et al.,
bioRxiv - Biochemistry 2021
Quote:
... coli expression by GENEWIZ. Expressed K-RAS proteins were full-length with the C-terminal two amino acids removed to mimic post-translational processing (Hancock et al. ...
-
No products found
because this supplier's products are not listed.
Steven J. Kunnen, et al.,
bioRxiv - Pharmacology and Toxicology 2024
Quote:
... in INVITROGRO HI medium (BioIVT, #Z99009) supplemented with TORPEDO antibiotics mix (BioIVT ...
-
No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
The in vitro kinase assay (HTRF KinEASE-STK S1, CisBio 62ST1PEB) was optimized to the linear reaction range of the enzymes Lats1 (Carna 01 −123 ...
-
No products found
because this supplier's products are not listed.
Taylor Moncrief, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... 0.4 μM of S1 Fragment (Rabbit Skeletal Muscle, Cytoskeleton #CS-MYS04) was incubated either alone or with 2 μM UNC-45B proteins for 10 minutes either at 42°C or on ice in Mg-buffer (10 mM PIPES-KOH ...
-
No products found
because this supplier's products are not listed.
Haijing Guo, Jen-Hsuan Wei, Joachim Seemann,
bioRxiv - Cell Biology 2020
Quote:
... 2 µM Phos-tag Biotin (ApexBio) and 2 ng/ml streptavidin-HRP (Jackson ImmunoResearch)] ...
-
No products found
because this supplier's products are not listed.
Joury S van ‘t Klooster, et al.,
bioRxiv - Biochemistry 2020
Quote:
... 10μM E-64 (Apollo Scientific, UK), 2 mM PMSF and protease inhibitor cocktail (cOmplete Mini EDTA-free™ ...
-
No products found
because this supplier's products are not listed.
Antwi-Boasiako Oteng, et al.,
bioRxiv - Physiology 2021
Quote:
... and cholesterol (Cholesterol E, Wako Diagnostics). Plasma alanine aminotransferase (ALT ...
-
No products found
because this supplier's products are not listed.
Brian Czaya, et al.,
bioRxiv - Cell Biology 2021
Quote:
... H&E or Masson’s trichrome (IDEXX) and used for representative images ...
-
No products found
because this supplier's products are not listed.
Dennis Segebarth, et al.,
bioRxiv - Neuroscience 2020
Quote:
Male 3-month-old 129S1/SvImJ (S1) mice (Charles River, Sulzfeld, Germany) were housed (four per cage ...
-
No products found
because this supplier's products are not listed.
Neha Upadhyay-Tiwari, et al.,
bioRxiv - Plant Biology 2024
Quote:
... S1) was obtained by forward genetic screen using an ethyl methanesulphonate (EMS)-mutagenized ProDH1pro:LUC2 population as previously described (20) ...
-
No products found
because this supplier's products are not listed.
Or-Yam Revach, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... S1 and S3 were banked using BAMBANKER freezing media (Bulldog Bio, BB02) and kept for downstream analysis by flow cytometry (see section ...
-
No products found
because this supplier's products are not listed.
Beltrán Ortolá, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... coli was co-electroporated (Eporator, Eppendorf) with p15LtRnlSm along with pLPP ...
-
No products found
because this supplier's products are not listed.
María Florencia Pignataro, et al.,
bioRxiv - Biochemistry 2022
Quote:
... coli BL21 (DE3) by BIO BASIC Inc (Markham ON ...
-
No products found
because this supplier's products are not listed.
Brian G. Pierce, et al.,
bioRxiv - Microbiology 2020
Quote:
The interaction of recombinant sE2 glycoproteins with CD81 and HMAbs in was measured using an Octet RED96 instrument and Ni2+-NTA biosensors (Pall ForteBio). The biosensors were loaded with 5 μg/mL of purified His6-tagged wild-type or mutant sE2 for 600 sec ...
-
No products found
because this supplier's products are not listed.
Miguel Hermida, et al.,
bioRxiv - Genomics 2022
Quote:
... E-96 mollusc DNA kit (Omega Bio-tek) following manufacturer recommendations ...
-
No products found
because this supplier's products are not listed.
Ankita B. Jaykumar, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... and mycoplasma-free (e-Myco Kit, Boca Scientific or Universal Mycoplasma Detection Kit 30-1012K ...
-
No products found
because this supplier's products are not listed.
Min Zeng, et al.,
bioRxiv - Synthetic Biology 2023
Quote:
... coli were 50 µg/ml kanamycin (GoldBio) or 100 µg/ml carbenicillin (GoldBio) ...
-
No products found
because this supplier's products are not listed.
Guangda Shi, et al.,
bioRxiv - Cell Biology 2021
Quote:
... 50 μM Phos-Tag reagent (Nard Institute AAL-107), and 100 μM MnCl2 ...
-
No products found
because this supplier's products are not listed.
Anna Jeffery-Smith, et al.,
bioRxiv - Immunology 2021
Quote:
... S1 (1μg/ml) and RBD (10μg/ml) and anti-human IgG (1μg/ml Jackson Immunoresearch) overnight at 4°C ...
-
No products found
because this supplier's products are not listed.
Enriqueta Garcia-Gutierrez, et al.,
bioRxiv - Microbiology 2019
Quote:
... 10 mg Strata–E C18 SPE column (Phenomenex, UK) pre-equilibrated with methanol and water ...
-
No products found
because this supplier's products are not listed.
Jason Derks, Nikolai Slavov,
bioRxiv - Systems Biology 2023
Quote:
... and mTRAQ-Δ8 mass tags (SciEx, 4440015, 4427698 and 4427700), respectively ...
-
No products found
because this supplier's products are not listed.
Xiangfeng Chen, et al.,
bioRxiv - Bioengineering 2022
Quote:
... and the EGM-e Bullet Kit was purchased from Lonza (USA). The Cell Proliferation Kit I (MTT ...
-
No products found
because this supplier's products are not listed.
Kui K. Chan, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and anti-HIS-FITC (chicken polyclonal, 1/100 dilution; Immunology Consultants Laboratory) secondary antibodies for 20 minutes at 4°C ...
-
No products found
because this supplier's products are not listed.
Lara K. Krüger, et al.,
bioRxiv - Cell Biology 2021
Quote:
... an TIRF-E motorized TIRF illuminator and an EMCCD Evolve camera (Photometrics). For excitation 491 nm 42 mW and 642 nm 480 mW (Gataca Systems ...