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Citations for Corning :
1 - 46 of 46 citations for MERS Coronavirus Spike Glycoprotein S1 His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... His-S1 or ecto-spike proteins were plated on half-area high-binding 96-well polystyrene plates (Corning) at 1 μg/mL or 0.5 μg/mL ...
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bioRxiv - Bioengineering 2021Quote: 6x-His tag antibodies were coated at 2 ug/mL in PBS onto 96-well half-area high binding plates (Corning, 3690) overnight at 4°C or 2 h at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 3% HI-FBS (Corning®), 1% (v/v ...
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bioRxiv - Microbiology 2021Quote: ... or ectodomain of trimeric Spike were immobilized onto 96-well plates (9018, Corning) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... 4% heat-inactivated FBS (HI-FBS, FBS from Corning, #35-010-CV ...
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bioRxiv - Microbiology 2020Quote: ... Escherichia coli was grown in Lysogeny Broth (LB) (Corning) medium under aerobic conditions at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli was grown in lysogeny broth (LB) (Corning) medium under aerobic conditions at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... The DNA pellet of dsDNA S1-Fc was dissolved in PBS buffer (Corning Cat.No.21-040-CM) at 2-2.5 μg/μl ...
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bioRxiv - Biochemistry 2020Quote: Recombinant SARS-CoV-2 Spike protein was coated onto high-binding 96-well plates (Corning, 3690) at 2 μg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... 10% heat inactivated fetal bovine serum (HI FBS) (USA Sourced, Corning, USA), 1x antibiotic/antimycotic (P/S ...
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bioRxiv - Immunology 2022Quote: ... and Omicron (BA.1) spike protein by coating of high-binding 96-half-well plates (Corning 3690) with 50 μl per well of a 1μg/ml indicated protein solution in Phosphate-buffered Saline (PBS ...
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bioRxiv - Cell Biology 2022Quote: ... as well standard tissue culture polystyrene (TCP) plates (E~GPa range) (Corning). Prior to seeding the cells ...
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bioRxiv - Immunology 2023Quote: ... 350 μg of spike/RBD encoding plasmid was mixed with 40 ml transfectagro™ (Corning 40-300-CV) and filtered using 0.22 μm Steriflip™ Sterile Disposable Vacuum Filter Units (MilliporeSigma™ SCGP00525) ...
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bioRxiv - Microbiology 2020Quote: ... Escherichia coli S17λpir was grown in Miller’s Lysogeny Broth (LB) (Corning) supplemented with ampicillin (100 μg/ml ...
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bioRxiv - Biochemistry 2020Quote: ... coli were immobilized on wells of 96 well EIA plate (Corning). After blocking with 1% BSA in TBST (20 mM Tris pH 7.6 ...
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli S17λpir was grown in Miller’s Lysogeny Broth (LB) (Corning) supplemented with ampicillin (100 µg/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... E-assembloids were transferred into TrypLE containing 1 mg/ml Dispase (Corning, 354235) and incubated for 30 min at 37°C ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were frozen in 10% DMSO/10% heat inactivated (HI) human AB serum (Corning) for later flow cytometry staining ...
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bioRxiv - Neuroscience 2021Quote: ... The iPSC lines used in this study (NSB2607-1-4 and NSB553-S1-1)75,76 were maintained in Matrigel (Corning, cat.# 354230) coated 6-well plates under NPC medium (DMEM/F12 (Life Technologies ...
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bioRxiv - Biochemistry 2022Quote: ... 25 µl of virus dilutions were combined with 25 µl of Spike sensor (diluted in PBS from 4X stock) in a 96-well white polystyrene microplates (Corning) and incubated for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... 350ug of the plasmids encoding spikes were transfected into 1L HEK-293F cells at 1 million cells/ml using Transfectagro (Corning) and 40K polyethylenimine (PEI ...
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bioRxiv - Biophysics 2022Quote: ... coli libraries were plated on eight each of LB square bioassay dishes (Corning) supplemented with 100 μg/ml carbenicillin and incubated at 37°C overnight ...
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bioRxiv - Immunology 2020Quote: ELISAs to evaluate antibodies binding to SARS-CoV-2 RBD and trimeric spike proteins were performed by coating of high binding 96 half well plates (Corning #3690) with 50 μL per well of a 1μg/mL protein solution in PBS overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: HT-29cf8 cells expressing the E-cadherin tension sensor were grown on Transwell inserts (Corning Incorporated). Inserts were cut out ...
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bioRxiv - Cancer Biology 2024Quote: ... E: Corning® Costar ® 96-well Clear Round Bottom Ultra-Low Attachment Microplates (Corning, #7007), F ...
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bioRxiv - Genomics 2021Quote: ... for imaging or poly-D-lysine coated 100 mm culture plates for Hi-C experiments (Corning BioCoat 356469), and maintained at 4° C for a few hours or overnight until nuclei were settled and adhering to the dish ...
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bioRxiv - Systems Biology 2019Quote: ... HT-1080 cells were cultured in DMEM Hi-glucose media (Corning Life Science, Cat. No. MT-10-013-CV) supplemented with 1% non-essential amino acids (NEAAs ...
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bioRxiv - Microbiology 2021Quote: ... Purified ZIKV E protein was diluted and added to 96-well microtiter plates (Corning-Costar, Corning, NY, USA) at 0.5 μg/well ...
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bioRxiv - Microbiology 2021Quote: ... Purified ZIKV E protein was diluted and added to 96-well microtiter plates (Corning-Costar, Corning, NY, USA) at 0.5 μg/well ...
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bioRxiv - Cancer Biology 2021Quote: HT-1080 and 293T cells were cultured in DMEM Hi-glucose medium (Cat# MT-10-013-CV, Corning Life Science) supplemented with 1% non-essential amino acids (NEAAs ...
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bioRxiv - Microbiology 2021Quote: ... supplemented with 10% (v/v) FBS-HI 24-72 h prior to infection using 150-mm tissue culture-treated plates (Corning) at 6×106 cells per dish (for mass-spectrometry (MS ...
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bioRxiv - Cancer Biology 2024Quote: ... 1×106 cells of E-cad+ or E-cad- MDA-1 MB-231 were resuspended in 100 μL of DPBS:Matrigel (1:1 ratio, #354234, Corning) and were injected into the second mammary fat pad of 5-week-old female NSG mice (n = 10) ...
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bioRxiv - Genomics 2023Quote: ... the puck was incubated for 7.5 mins (TAGS beads) or 5 mins (SLAC beads) and then placed into a 12-well plate (Corning, 3512). Using a 200 µL pipette ...
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bioRxiv - Cell Biology 2020Quote: ... The SVF from interscapular brown fat pads of 6 – 8 mice were then resuspended in 6 ml DMEM supplemented with 10 % HI NCBS and plated on 3 wells of a six well plate (Corning, #3506). After 4 and 24 h ...
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bioRxiv - Microbiology 2020Quote: ... We diluted cells to 3.3 x 106 cells/mL RPMI/10%HI-FBS and transferred 900 µL each to 2 mL external thread cryogenic vials (Corning, Corning, NY) for each experimental condition ...
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bioRxiv - Microbiology 2022Quote: ... were maintained in Modified Eagle’s Medium with Earle’s Balanced Salt Solution and L-glutamine (MEM-E) supplemented with 10% fetal bovine serum (FBS) (Corning CellGro). Cells were maintained at 37 °C in humidified incubators with 5% CO2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Single-cell–dissociated esophageal epithelial cells (1000 cells) were suspended in 8 μL of E-MEOM and 12 μL of pre-thawed Matrigel (Corning) on ice and seeded in the centers of a well to create a Matrigel dome ...
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bioRxiv - Microbiology 2020Quote: ... We diluted cells to 3.3 x 106 cells/mL RPMI/10%HI-FBS and transferred 900 µL each to 2 mL external thread cryogenic vials (Corning, Corning, NY) for each experimental condition ...
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bioRxiv - Biochemistry 2022Quote: ... OX40 overexpressing Jurkat cells engineered with an NFκB luciferase reporter were seeded at 80,000 cells/well in 20 µl RPMI containing 1% L-glutamine and 10% HI FBS in a 384-well tissue culture plate (Corning Inc., 3985BC). Anti-OX40 antibody formats were serially diluted three-fold in media starting at 30 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... Plant-expressed 6-His tagged ZE3 at 50 ng/well was bound to 96-well high-binding polystyrene plates (Corning Inc, Corning, NY, USA), and the plates were blocked with 5% nonfat dry milk in PBST ...
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bioRxiv - Molecular Biology 2023Quote: ... the optimized reaction mixture (1.5 nM of 6His-MSN and 25 nM of FITC-CD44 peptide in the presence of anti-His-Tb at final 1:1000 dilution) was dispensed at 5 µL per well into 1536-well black plates (Corning Costar, #3724). 0.1 µL of library compound dissolved in DMSO was added using a pin tool integrated with the Biomek NX automated liquid handling workstation (Beckman Coulter) ...
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bioRxiv - Immunology 2024Quote: ... BMDMs were then subjected to serum shock by replacing serum-free media with RPMI supplemented with 50% (v/V) HI horse serum (Corning, CAT#35030CV) at 37⁰C in air enriched with 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Bci-NS1.1 were plated (7.5 E + 04 cells/well) on rat-tail collagen type 1-coated permeable transwell membrane supports (6.5 mm; Corning, cat no. 3470), and immersed apically and basolaterally in Pneumacult Ex Plus medium (StemCell ...
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bioRxiv - Immunology 2020Quote: ... Plant-expressed 6-His tagged ZE3 at 50 ng/well was bound to 96-well high-binding polystyrene plates (Corning Inc, Corning, NY, USA), and the plates were blocked with 5% nonfat dry milk in PBST ...
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bioRxiv - Systems Biology 2024Quote: ... cells were switched into either serum-free DMEM (SFM) or DMEM with 10% HI-FBS (DFBS) or Corning® Intestinal Epithelium Differentiation Medium (Corning product number 355357) (DM) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... or 17-beta estradiol (E) or remained empty and sealed at both ends with medical-grade silicone adhesive (Dow Corning Corp., Midland, MI). All sham surgery animals received empty Silastic capsules ...