-
No products found
because this supplier's products are not listed.
Yanqin Ren, et al.,
bioRxiv - Microbiology 2020
Quote:
... anti-human HLA-DR (clone L243, Biolegend, 1:200), anti-human BCL-XL (clone 7B2.5 ...
-
No products found
because this supplier's products are not listed.
Jihoon William Lee, et al.,
bioRxiv - Immunology 2024
Quote:
... captured HLA-A2 SCD-peptide complex with Ni-NTA agarose beads (Invitrogen) in imidazole equilibration buffer (10mM imidazole ...
-
No products found
because this supplier's products are not listed.
I Sánchez-Cerrillo, et al.,
bioRxiv - Immunology 2022
Quote:
... rabbit anti-human HLA-DR (abcam), as primary antibodies ...
-
No products found
because this supplier's products are not listed.
Ieva Bagdonaite, et al.,
bioRxiv - Microbiology 2022
Quote:
... HEK293 WT (Sigma Cat. Nr. 85120602), HEK293 GALNT1 KO (Narimatsu et al. ...
-
No products found
because this supplier's products are not listed.
Yong Zhang, et al.,
bioRxiv - Biochemistry 2020
Quote:
... via baculovirus and S protein (S1 and RBD, His tag) expressed in human embryonic kidney cells (HEK293) were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
-
No products found
because this supplier's products are not listed.
Angel Charles, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... FITC Anti-Human HLA-A2 from BD Biosciences ...
-
No products found
because this supplier's products are not listed.
Renpeng Ding, et al.,
bioRxiv - Immunology 2020
Quote:
HLA-A*0201-restricted MART-1 peptide ELAGIGILTV) was synthesized by GenScript (Nanjing, China). Peptide was stored at 10 mg/ml in 100% dimethyl sulfoxide (DMSO ...
-
No products found
because this supplier's products are not listed.
David S. Yang, et al.,
bioRxiv - Biophysics 2020
Quote:
... containing N-terminal 6-His-WT-p53 (1-303) (Addgene, 24859), was used to express p53 ...
-
No products found
because this supplier's products are not listed.
Amrita Rai, et al.,
bioRxiv - Biochemistry 2024
Quote:
... and Protein Complex suites (Qiagen). The sitting-drop vapor diffusion method was employed ...
-
No products found
because this supplier's products are not listed.
Nicholas J. Rettko, et al.,
bioRxiv - Immunology 2023
Quote:
... anti-human HLA-B (Proteintech, 17260), anti-human Glut1 (Abcam ...
-
No products found
because this supplier's products are not listed.
Seong Ho Hong, et al.,
bioRxiv - Biochemistry 2023
Quote:
... The SKP1 containing SCF complex was reconstituted by utilizing stoichiometric amount of the following recombinant proteins: Recombinant Human CUL1/RBX1 Neddylated Complex Protein (R&D systems, E3-411-025), Recombinant Human FBXO7 GST (N-Term ...
-
No products found
because this supplier's products are not listed.
Jennifer G. Abelin, et al.,
bioRxiv - Cancer Biology 2022
Quote:
During HLA complex capture a 10μm PE fritted plate (Agilent, S7898A) was cut in half ...
-
No products found
because this supplier's products are not listed.
Cecilia Piergentili, et al.,
bioRxiv - Biochemistry 2020
Quote:
... Enc:EncFtn protein complex sample was loaded onto a 20 ml anion exchange column (Hi-Prep Q HP, GE Healthcare) pre-equilibrated with Buffer QA (50 mM Tris ...
-
No products found
because this supplier's products are not listed.
Anny Gravdal, et al.,
bioRxiv - Cell Biology 2020
Quote:
Human Embryonic Kidney 293 (HEK293) cells (Clontech Laboratories) were used for most experiments described in this study ...
-
No products found
because this supplier's products are not listed.
Mingming Ma, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... The protein concentration of exosomes was determined by using the BCA protein determination kit (BIO-RAD, 500-0201). Western blotting and flow cytometry were used to detect exosome markers ...
-
No products found
because this supplier's products are not listed.
Ya-Juan Wang, et al.,
bioRxiv - Cell Biology 2022
Quote:
... The protein complex was isolated by immunoprecipitation using a mouse anti-His antibody (Cell Signaling, catalog #: 2366S) followed by SDS-PAGE and Western blot analysis with a rabbit anti-GABAA receptor α1 subunit antibody (R&D Systems ...
-
No products found
because this supplier's products are not listed.
Maitreyi Rathod, et al.,
bioRxiv - Cell Biology 2023
Quote:
... phosphorylated using T4 PNK enzyme (#0201S NEB, Ipswich, MA USA), annealed and ligated into Esp3I-digested (#R0734L NEB ...
-
No products found
because this supplier's products are not listed.
Robert W. Murdoch, et al.,
bioRxiv - Microbiology 2021
Quote:
... and digested with sequencing grade trypsin (Promega, 1:50 trypsin- to-protein [wt/wt]) (Yang et al. ...
-
No products found
because this supplier's products are not listed.
Irina Buckle, et al.,
bioRxiv - Immunology 2022
Quote:
... human IL-12p70 (HEK293) (Peprotech) and 50ng/ml recombinant human IL-18 (MBL International ...
-
No products found
because this supplier's products are not listed.
Yadira M. Soto-Feliciano, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... Human HEK293 cells were maintained in DMEM (Corning) supplemented with 10% FBS (Gibco) ...
-
No products found
because this supplier's products are not listed.
Wei Kong, et al.,
bioRxiv - Plant Biology 2021
Quote:
... His-tagged proteins were detected by a mouse anti-His antibody (sc-8036, 1:3000, Santa Cruz Biotechnology). GST-tagged proteins were detected by a mouse anti-GST antibody (sc-138 ...
-
No products found
because this supplier's products are not listed.
Sandro Trova, et al.,
bioRxiv - Microbiology 2023
Quote:
... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
-
No products found
because this supplier's products are not listed.
Adel Avetisyan, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... His-tagged proteins in soluble fraction were purified using cOmplete His-Tag Purification Columns (Roche). The columns were washed with 10 column volumes of wash buffer 1 (20 mM Tris ...
-
No products found
because this supplier's products are not listed.
Subeena Sood, et al.,
bioRxiv - Immunology 2023
Quote:
HEK293 cells expressing human ACE2 and TMPRSS2a (Invivogen) were seeded at 20,000 cells/well in a gelatin-coated 96-well plate (Corning ...
-
No products found
because this supplier's products are not listed.
Kyle A. Campbell, et al.,
bioRxiv - Genomics 2024
Quote:
... the cytotrophoblast fraction was incubated for 10 minutes at 4 °C in the dark with 1:11 REAfinity™ allophycocyanin (APC)-conjugated anti-human leukocyte antigen (HLA)-ABC antibody (APC-anti-HLA-ABC, Miltenyi Biotec, #130-101-467, lot #5191002371). The Hofbauer/fibroblast fraction was incubated for 10 minutes at 4 °C in the dark with 1:11 REAfinity™ allophycocyanin-conjugated anti-epidermal growth factor (EGFR ...
-
No products found
because this supplier's products are not listed.
Debanjali Bhattacharya, et al.,
bioRxiv - Molecular Biology 2024
Quote:
The plasmid encoding full-length WT human RNF20 was purchased from Origene and cloned into pcDNA3β expression vector by PCR amplification ...
-
No products found
because this supplier's products are not listed.
Tobias Moll, et al.,
bioRxiv - Neuroscience 2022
Quote:
HEK293 Sham and HEK293_GLT8D1-WT/R92C stable cell lines were cultured in 10mL DMEM (Lonza) supplemented with 10% (v/v ...
-
No products found
because this supplier's products are not listed.
T.A. van Wageningen, et al.,
bioRxiv - Neuroscience 2020
Quote:
... sections were washed in TBS and incubated for 1 hr with horseradish peroxidase-labeled avidin-biotin complex (ABC complex, 1:400, Vector Labs) at RT ...
-
No products found
because this supplier's products are not listed.
Vincent E. Provasek, et al.,
bioRxiv - Biochemistry 2024
Quote:
... WT human neural stem cells were (Jackson Labs) maintained on Geltrex LDEV-Free in Stem Pro medium (Gibco) ...
-
No products found
because this supplier's products are not listed.
D. Azimova, et al.,
bioRxiv - Microbiology 2021
Quote:
... We verified the identity of the proteins in the complex by western blot using native antibody for Cbp1 and a mouse anti-his tag antibody (ABclonal AE003) for Yps3-6xhis.
-
No products found
because this supplier's products are not listed.
Joseph J. Zhao, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... pups were irradiated with sublethal dose (1 Gy) and thereafter engrafted with 1×105 human CD34+ cord blood cells (HLA-A24:02, Stemcell Technologies) through intrahepatic injection ...
-
No products found
because this supplier's products are not listed.
Deepti Mudaliar, et al.,
bioRxiv - Biochemistry 2024
Quote:
... Recombinant Human CKII alpha prime polypeptide His Protein (NBP2-22736) was purchased from Novus Biologicals (Centennial, CO, USA). CK2 Substrate (synthetic peptide RRRADDSDDDDD ...
-
No products found
because this supplier's products are not listed.
Kuan-Yi Lu, et al.,
bioRxiv - Microbiology 2020
Quote:
... Protein syntheses were induced by 2% (wt/vol) galactose (VWR International) at 30 °C for 4 h at 250 rpm when the OD600 reached 0.8–1.0 ...
-
No products found
because this supplier's products are not listed.
Dmitry Shvarev, et al.,
bioRxiv - Biophysics 2022
Quote:
... 3 μl of 0.6–0.9 mg/ml of freshly purified wt HOPS complex were applied onto glow-discharged CF grids (R1.2/1.3) (EMS) and immediately plunge-frozen in liquid ethane using a Vitrobot Mark IV (Thermo Fisher Scientific ...
-
No products found
because this supplier's products are not listed.
Sam C. Nalle, et al.,
bioRxiv - Immunology 2020
Quote:
HEK293 cells stably expressing fluorescently-tagged AQP3 (an AQP3-DsRed2 fusion protein) were incubated with 1 μm latex beads (Polysciences) for 2 hours prior to fixation with 2% paraformaldehyde (PFA)/0.2% glutaraldehyde ...
-
No products found
because this supplier's products are not listed.
Matthew Y. Otsuka, et al.,
bioRxiv - Biochemistry 2024
Quote:
... timed pregnant CD-1 WT mice were purchased from Charles River Laboratories (Wilmington ...
-
No products found
because this supplier's products are not listed.
Yu Huan Jiang, et al.,
bioRxiv - Immunology 2023
Quote:
... anti-HLA-DR(Beckman Coulter, Villepinte, France). Isotype controls with irrelevant specificities were included as negative controls ...
-
No products found
because this supplier's products are not listed.
Shraddha Pai, et al.,
bioRxiv - Genomics 2021
Quote:
We downloaded publicly-available Hi-C data from human prefrontal cortex tissue23,24 (Illumina HiSeq 2000 paired-end raw sequence reads ...
-
No products found
because this supplier's products are not listed.
Sonali Jindal, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... human COX2 protein (Cayman Chemical, 60122, 4ng), and 25μg cell line lysates in RIPA buffer were separated by WES automated gel electrophoresis System (Protein Simple ...
-
No products found
because this supplier's products are not listed.
Antonio Santos-Peral, et al.,
bioRxiv - Immunology 2023
Quote:
... followed by an incubation with 1 µg/ml of recombinant sE WT or dimer-mutants or anti-human IgG-HRP (Jackson ImmunoResearch, 109-035-088, 1:5000) in PBS+0.5% FCS for 2 h at RT ...
-
No products found
because this supplier's products are not listed.
Sravya Kurapati, et al.,
bioRxiv - Immunology 2024
Quote:
... ýIII-Tubulin (1:250) and human HLA-DR (1:250, LN-3, Leica/Novocastra) followed by staining with a cocktail of DAPI and fluorochrome-conjugated secondary antibodies and mounting on histological slides.
-
No products found
because this supplier's products are not listed.
Vignesh Ramesh, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... transfection complex was prepared by mixing 50 nM of SMARTPool ON-TARGETplus Human siRNAs (horizon, PerkinElmer) with 3 μl of Lipofectamine RNAiMAX transfection reagent (ThermoFisher Scientific ...
-
No products found
because this supplier's products are not listed.
Miao-Hsi Hsieh, et al.,
bioRxiv - Microbiology 2020
Quote:
... SARS-CoV-2 S1 protein containing a C-terminal His-tag (Acro; S1N-C52H3) (5 μg/ml) was tagged with anti-His antibody (Genetex; GT395) (1:100 ...
-
No products found
because this supplier's products are not listed.
Chunyan Wang, et al.,
bioRxiv - Microbiology 2020
Quote:
... Antibody binding to the spike proteins was determined using a 1:2000 diluted HRP conjugated goat anti-human IgG (ITK Southern Biotech) for 1 h at RT and tetramethylbenzidine substrate (BioFX) ...
-
No products found
because this supplier's products are not listed.
Vitaly Zimyanin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... InjectionB - Inhibition of Complex V: 1 µM Oligomycin A (Tocris); InjectionC – uncoupling ...
-
No products found
because this supplier's products are not listed.
Ben Vanderkruk, et al.,
bioRxiv - Cell Biology 2022
Quote:
... and an equal mass of WT and KO nuclear lysate (∼30 μg protein) was immunoprecipitated by 1 μg WDR5 (Bethyl, A302-429A) or normal rabbit IgG (Millipore ...
-
No products found
because this supplier's products are not listed.
Patrick Blatt, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Rabbit anti-His (1:000, Rockland Inc., 600-401-382), Mouse anti-Fibrillarin (1:25 ...
-
No products found
because this supplier's products are not listed.
Babita Sharma, et al.,
bioRxiv - Cancer Biology 2024
Quote:
Proteins (200 mL each from WT and KO samples, in 1.5 mL microcentrifuge tubes (Eppendorf)) ...
-
No products found
because this supplier's products are not listed.
Florentina Negoita, et al.,
bioRxiv - Biochemistry 2024
Quote:
... The protein-antibody complex on the membranes were visualized by Odyssey XF Imager (LI-COR Biotech, LLC, Germany) after incubation of the membrane with the enhanced chemiluminescence substrate (Millipore ...
-
No products found
because this supplier's products are not listed.
Marisol Sampedro-Castañeda, et al.,
bioRxiv - Neuroscience 2023
Quote:
... mouse anti Cav α1E 1:1000 (Synaptic Systems, 152411, human neurons), rabbit anti CDKL5 1:2000 (Atlas ...