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Citations for Agilent :
1 - 50 of 3546 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: During HLA complex capture a 10μm PE fritted plate (Agilent, S7898A) was cut in half ...
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bioRxiv - Neuroscience 2020Quote: ... human embryonic kidney cells 293 (HEK293; Agilent #240073) were calcium phosphate-transfected with the recombinant AAV2 plasmid and a 3-helper system ...
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bioRxiv - Cancer Biology 2019Quote: ... mouse anti-human HLA-DRα mAb (clone, TAL. 1B5; Dako Cytomation), mouse anti-human CD163 mAb (clone ...
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bioRxiv - Immunology 2020Quote: ... mouse-anti-human EGFR (Dako, DAK-H1-WT), and CLTX-biotin ...
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bioRxiv - Biochemistry 2019Quote: ... carrying the human WT RAD51 (WT-pET11d) was used as a template for the PCR-mediated QuikChange site-directed mutagenesis (Agilent Technologies) to introduce F86E substitution (F86E-pET11d ...
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bioRxiv - Neuroscience 2021Quote: ... Mamu-DR (monoclonal mouse – anti-human HLA-DR/DQ-IgG1, clone CR3/43, cat. no. M077501-2, Agilent Technologies), 1:150 ...
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bioRxiv - Neuroscience 2019Quote: ... the avidin-biotin-peroxidase complex (1:300; Dako, Glostrup, Denmark) was applied for 1 h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Biotinylated HLA-A3 monomers were generated and conjugated to streptavidin PE (Agilent, PJRS25-1) as described [41].
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with a streptavidin-HRP complex (1:1000, Dako). Immunoreactivity was visualized using DAB (0.4 mg/ml ...
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bioRxiv - Neuroscience 2023Quote: Plasmid construct (pRK172-human-lil-synuclein) encoding WT-human-α-synuclein was expressed in BL21-CodonPlus (DE3-RIL) cells (Agilent, Cat# 230245-41). Protein expression was induced with 0.1 mM IPTG at cell density OD (600 nm ...
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bioRxiv - Cell Biology 2021Quote: ... The mGli2-His recombinant protein was expressed in BL21 (DE3) pLysS cells (Stratagene) and purified under denaturing condition (8 M urea ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were then loaded in equal volumes into 24 wells of an Agilent 3100 OFFGEL Fractionator using a high-resolution fractionation kit (Agilent Cat#5067-0201). Each sample ...
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bioRxiv - Plant Biology 2020Quote: ... pAD-WT and pBD-WT from the HybriZAP 2.1 kit (Stratagene, USA) were used as a control of positive interaction (C+) ...
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bioRxiv - Immunology 2021Quote: ... a monoclonal mouse antibody against the intracellular tail of human HLA-DR α chain was used (Clone TAL.1B5, # M0746, Agilent, Santa Clara, CA, USA). Detection of MHC-I on the surface of cells was performed using a monoclonal mouse antibody against devil beta-2-microglobulin (B2M ...
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bioRxiv - Neuroscience 2023Quote: ... were transfected into HEK293 cells (AAV293, Stratagene). After 3 days ...
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bioRxiv - Neuroscience 2023Quote: ... were transfected into HEK293 cells (AAV293, Stratagene). After three days ...
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bioRxiv - Molecular Biology 2020Quote: ... The free HSF2BP protein as well as the complex between ARM and F15X were analyzed using a Bio SEC-3 column (Agilent) equilibrated in 25 mM Tris-HCl buffer ...
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bioRxiv - Biochemistry 2023Quote: ... An alanine mutant library of the SNAP-tagged human truncated mGlu5 receptor (mGlu5-Δ856, designated as WT in the following experiments) was generated using Quick-change strategy (Agilent technologies) and verified by sequencing (Eurofins Genomics)11 ...
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bioRxiv - Biochemistry 2022Quote: ... cholerae TrcP was sub-cloned into a custom pET vector and expressed as a 6× His-tagged N-terminal human SUMO2 fusion in E coli BL21 RIL bacteria (Agilent). A 50 ml starter culture grown overnight at 37°C in MDG medium (1.5% Bacto agar ...
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bioRxiv - Biochemistry 2023Quote: ... The plasmid carrying the WT Cagα protein pHTCagα was amplified using the QuikChange II site-directed mutagenesis kit (Agilent) using 33 bp primers (suppl ...
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bioRxiv - Neuroscience 2021Quote: ... We used additional microglial markers including mouse anti-MHC-II/HLA-DP/DQ/DR (1:250, M077501-2, Agilent, UK), rabbit anti-PU.1 (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... template WT plasmid was digested by the addition of 1 μL DpnI (Agilent Technologies) for 5 min at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Anti-human tau (Dako, 1:10,000), the phosphorylation specific anti-tau antibody Ser396/Ser404 (PHF1 ...
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bioRxiv - Cancer Biology 2021Quote: ... human CD45 (Dako M0701, 1:100), and human CD20 (Dako M0755 ...
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bioRxiv - Cancer Biology 2023Quote: ... human vimentin (1:4000; M0725, DakoCytomation), breast cancer resistance protein (BCRP ...
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bioRxiv - Biochemistry 2020Quote: Nhp6 was expressed as a N-terminal 6x His-tag fusion protein in E.coli BL21-CodonPlus (DE3)-RIL cells (Agilent). A colony of cells freshly transformed with plasmid p1035 was grown in 3 L of LB supplemented with 50 μg/mL ampicillin and 34 μg/mL chloramphenicol at 37°C ...
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bioRxiv - Immunology 2020Quote: ... The primary antibodies used for immunohistochemistry were: antihuman HLA-DR (referred to from here as MHC-II) (Dako M0746; 1:40); anti-human CD3 (Dako A0452 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-HLA-DR (clone TAL.1B5, Dako Agilent, Santa Clara, CA, USA); rabbit anti-IBA1 (Polyclonal IgG ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-HLA-DR (clone TAL.1B5, Dako Agilent, Santa Clara, CA, USA); rabbit anti-IBA1 (Polyclonal IgG ...
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bioRxiv - Physiology 2020Quote: ... followed by incubation in streptavidin peroxidase complex (DAKO-LSAB) for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated in avidin-biotin complex (ABC, Dako) for 30 minutes and the antigen-antibody reaction was visualized using di-aminobenzidine (DAB ...
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bioRxiv - Biophysics 2021Quote: ... The plasmid encoding the protein fused with a His-tag at the N-terminus was transformed in BL21 DE3 pLysS strains (Agilent). Recombinant αE-catenin was expressed via isopropyl 1-thio-β-d-galactopyranoside (IPTG,Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... constructs containing DNA encoding 8X-His-tagged CHI-domain-containing proteins were transformed into BL21-CodonPlus (DE3)-RIPL Competent Cells (Agilent) for recombinant protein expression ...
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bioRxiv - Cell Biology 2021Quote: ... We performed exome sequencing in the child with neonatal diabetes and his consanguineous parents using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb). We analyzed the data with our established pipeline in the institute (Kuhnen ...
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bioRxiv - Developmental Biology 2023Quote: ... We performed exome sequencing in the child with diabetes and his consanguineous parents and sibling using the Agilent SureSelect Human All Exon Kit (Agilent SureSelect v4, 50Mb) and next-generation sequencing (Hiseq ...
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bioRxiv - Cancer Biology 2023Quote: ... the plasmids containing the C- and N-terminally His-tagged human ACSS2 DNA were transformed into BL21 (DE3) RIL competent cells (Agilent Technologies, Wilmington, DE) and were expressed in auto-inducing media ZYP-5052 overnight at 15°C with shaking at 225 rpm ...
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bioRxiv - Immunology 2020Quote: ... anti-human CD3 (Dako A0452; 1:300); anti-human periaxin (PXN ...
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bioRxiv - Cancer Biology 2021Quote: ... and human CD20 (Dako M0755, 1:1000) on the Leica Bond RX automated staining as previously described for the CAV1 IHC method ...
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bioRxiv - Neuroscience 2023Quote: ... and anti-human Tau (1:500, Dako). After incubation with the primary antibodies ...
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bioRxiv - Immunology 2023Quote: ... rabbit anti-human C3d (1:200; Dako), and rabbit anti-human C5b-9 (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-coding genes were captured using SureSelectXT Human All Exon V5 probes (Agilent) and sequenced on Illumina HiSeq 4000 using 100bp paired end read protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by incubation with HRP-conjugated streptavidin–biotin complex (DAKO). Substrate was developed with DAB (DAKO)and pictures were produced using a Nikon Eclipse E800microscope with a Nikon DXM1200 digital camera (Nikon ...
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bioRxiv - Neuroscience 2019Quote: ... and RepCap5 (Applied Viromics) were transfected to HEK293 cells (AAV293, Stratagene). After 3 days of incubation ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-human prealbumin/TTR (1:1000, 2.0 g L−1, Dako), anti-DNAJB11/ERdj3 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human prealbumin/TTR (1:1000, 2.0 g L−1, Dako), anti-DNAJB11/ERdj3 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Rabbit anti–human vWF (A0082, Agilent, 1:300). Primary antibodies were detected with the corresponding Alexa Fluor -555 ...
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bioRxiv - Bioengineering 2022Quote: ... human CD31 (mouse, 1:200, clone JC70A, Agilent), mouse CD31 (rat ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... human vimentin (1:4,000; M0725; DakoCytomation; Glostrup, Denmark), P-gp (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... Rabbit anti-human GFAP (1:400, Dako, Z0334), Rabbit anti-human SLC1A3 (1:500 ...
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bioRxiv - Microbiology 2020Quote: ... the sections were incubated with a streptavidin-biotin-HRP complex (DakoCytomation) and the brown color was developed with diaminobenzidine tetrahydrochloride (DAB ...