Labshake search
Citations for Eppendorf :
1 - 50 of 565 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2020Quote: ... but all reagents (DNA, RNP complex, and Eppendorf tubes) were kept ice cold before reactions were initiated ...
-
bioRxiv - Biophysics 2019Quote: ... The His-SUMO solubility tag was cleaved using His-tagged SUMO protease (from RGO) by incubating at 4°C overnight while gently rocking in protein lobind tubes (Eppendorf). The flow through of the second affinity step was collected and concentrated prior to loading onto a Superdex S200 (26/60 ...
-
bioRxiv - Cell Biology 2023Quote: ... The sgRNA/Cas9 complex was pipetted into a Femtotips needle (Eppendorf, 5242957000) and connected to a microinjector (Eppendorf ...
-
bioRxiv - Molecular Biology 2024Quote: A total of 350,000 HEK293-T cells were seeded in each well of 6-well plates (#EP0030720113, Eppendorf). Transfection was performed the day after at 40-50% cell confluence ...
-
bioRxiv - Immunology 2023Quote: ... cells were labeled with 50 nM of biotinylated human ACE2 protein (Acro Biosystems, AC2-H82E6) for 30 minutes at 4°C at 700 RPM on a shaker (Eppendorf, ThermoMixer C). The cells were subsequently washed ...
-
bioRxiv - Developmental Biology 2021Quote: ... The obtained complex was then microinjected into fertilized eggs (B6D2F1) using a programmable microinjector (FemtoJet 4i, Eppendorf, Hamburg, Germany). The microinjected eggs were then transplanted into the oviduct ampulla of pseudopregnant mice (ICR ...
-
bioRxiv - Microbiology 2023Quote: ... epidermidis 1457 (WT, ΔatlE, and ΔicaADBC) (kindly donated by Prof. Holger Rohde, Universitatsklinikum Hamburg-Eppendorf, Hamburg, Germany) and S ...
-
bioRxiv - Biochemistry 2023Quote: ... and the protein concentration determined by nanodrop (assuming an extinction coefficient of εMolar = 46410 M−1cm−1. The protein solutions were moved into 1 mL aliquots in 1.5 mL Eppendorf tubes ...
-
bioRxiv - Biophysics 2020Quote: ... WT and pY39 α-syn are incubated at 37 °C with constant agitation at 900 rpm in ThermoMixer (Eppendorf) for a week ...
-
bioRxiv - Microbiology 2022Quote: ... trypsin was inactivated with 1:1 complete media and the cell suspension was collected into a 5mL protein-low bind centrifuge tube (Eppendorf). To remove residual trypsin ...
-
bioRxiv - Neuroscience 2023Quote: ... The 1 ml of PBS with the resuspended cells was transferred to a 1·5 ml Protein LoBind tube (Eppendorf), centrifuged at 3000 x g for 6 min at 4 °C ...
-
bioRxiv - Cancer Biology 2020Quote: ... Protein extracts were stored in Protein LoBind tubes (Eppendorf) at −80°C ...
-
bioRxiv - Biochemistry 2020Quote: ... Protein samples were aliquoted into Protein LoBind tubes (Eppendorf) and polymerised at 30°C quiescently for at least 48 hours ...
-
bioRxiv - Biochemistry 2020Quote: ... Deep well 1-mL 96 well plate (Protein LoBind) was purchased from Eppendorf (Hauppauge, NY). Recombinant mouse HEXB protein (His Tag ...
-
bioRxiv - Cell Biology 2023Quote: 1 ml of concentrated protein (1mg/ml) was aliquoted into 1.5 ml tubes (LoBind, Eppendorf) and kept on Eppendorf ThermoMixer C at 37°C with 1500 rpm for 48 hr ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 μM purified TDP-43-MBP-His6 variants (WT, 5D, 12D, 12A) were set up in low binding tubes (Eppendorf) in 35 μl aggregation buffer (50 mM Tris pH 8.0 ...
-
bioRxiv - Developmental Biology 2022Quote: ... Approximately 30 mycn mutant or WT zebrafish embryos at 3 dpf were transferred to 1.5mL Low binding microcentrifuge tubes (Eppendorf 022431021). Trypsin-EDTA solution (Beyotime ...
-
bioRxiv - Immunology 2021Quote: RBD219-WT and RBD219-N1C1 were expressed at the 5 L scale using a Celligen 310 benchtop fermentation system (Eppendorf). For the RBD-WT ...
-
bioRxiv - Molecular Biology 2024Quote: ... Precleared protein extracts were transferred to Protein Lobind tubes (Eppendorf) and incubated with pre-washed 50 μl of magnetic-streptavidin beads (MyOne C1 ...
-
bioRxiv - Biophysics 2020Quote: ... Tween treated Protein LoBind tubes (Protein LoBind Tubes (1.5 ml, Eppendorf)) were incubated for 4 hours with 2% aqueous Tween solution (Tween20 ...
-
bioRxiv - Cell Biology 2021Quote: ... of AAV1-Cpne4 or AAV1-eGFP control virus were done in P0 or P14 Brn3bCre/WT mice eyes using glass capillaries fitted onto a Femtojet device (Eppendorf, Enfield, CT), as previously described ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... Protein LoBind tubes (Eppendorf) and epT.I.P.S ...
-
bioRxiv - Cell Biology 2020Quote: ... protein Lobind tubes (Eppendorf). Elution was repeated and the eluates were pooled ...
-
bioRxiv - Microbiology 2022Quote: ... Protein LoBind tubes (Eppendorf) minimized potential adsorption of protein ...
-
bioRxiv - Neuroscience 2020Quote: ... was added to a reagent-to-antibody molar ratio 10:1 in Protein LoBind tubes (Eppendorf, Hamburg, Germany), and the solutions were incubated for 1 h at room temperature under constant shaking at 500 rpm ...
-
bioRxiv - Plant Biology 2023Quote: ... The protein samples were centrifuged at maximum speed for 1 min in an Eppendorf™ 5424 Microcentrifuge (Eppendorf). The proteins were separated on 10% SDS polyacrylamide gels and transferred onto Immuno-Blot PVDF membrane (BioRad ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein-low binding tubes (Eppendorf) were used to make serial dilutions of BODIPY-cyclopamine.
-
bioRxiv - Physiology 2021Quote: ... in protein LoBind tubes (Eppendorf). Probes were then homogenized at 4°C in Bioruptor Pico sonicator (Diagenode) ...
-
bioRxiv - Neuroscience 2024Quote: ... protein low-binding tubes (Eppendorf) were used ...
-
bioRxiv - Biophysics 2024Quote: ... in Protein LoBind Tubes (Eppendorf). Protein levels were normalized via Bradford assay (Bio-Rad ...
-
bioRxiv - Neuroscience 2022Quote: 30 asteroids per condition were pooled in a 1.5 mL Protein LoBind Eppendorf (Eppendorf cat#022-43-108-1) and allowed to settle ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein amounts were assessed using a Pierce BCA protein assay kit and spectrophotometer (Eppendorf BioPhotometer). Equal amounts of protein (15 μg ...
-
bioRxiv - Biophysics 2021Quote: ... at a final ratio of 2.5 nmol DSS per 1 µg protein for 30 min at 37 °C while shaking at 650 rpm in a Thermomixer (Eppendorf). After quenching by addition of ammonium bicarbonate to a final concentration of 50 mM and incubation for 10 min at 37 °C ...
-
bioRxiv - Cell Biology 2021Quote: ... after which the protein samples were denatured at 650 rpm for 1 h at 37°C using a Thermomixer (Eppendorf). Sample reduction and alkylation were individually performed by adding 10 mM dithiothreitol and 40 mM iodoacetoamide ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The fractions with highest concentrations (200–400 μg mL−1) were stored as 5–10 μL aliquots in Protein LoBind tubes (Eppendorf) at –80 °C.
-
bioRxiv - Neuroscience 2022Quote: At time of collection asteroids were transferred to a 1.5 mL Protein LoBind Eppendorf (Eppendorf cat#022-43-108-1) and allowed to settle ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1 mM β-glycerol phosphate and 2.5 mM Na2H2P2O7).) and cells scraped and transferred into Protein Lo-bind tubes (Eppendorf). Samples were sonicated for 10 cycles (30s on & 40s off ...
-
bioRxiv - Neuroscience 2020Quote: ... The supernatant with eluted biotinylated proteins was carefully transferred to a low-protein-binding tube (Eppendorf) with a 30g needle and stored at −80°C.
-
bioRxiv - Cell Biology 2020Quote: ... transferred to protein LoBind tubes (Eppendorf), and minced ...
-
bioRxiv - Molecular Biology 2020Quote: ... into protein LoBind tubes (Eppendorf, Germany). Just before encapsulation ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Low protein binding tubes (022431081, Eppendorf) were used for sample preparation ...
-
bioRxiv - Biochemistry 2021Quote: ... Protein LoBind® tubes from Eppendorf; Trypsin (V5111 ...
-
bioRxiv - Biophysics 2022Quote: ... aliquoted into Protein LoBind tubes (Eppendorf), and stored at 4°C (maximum 48 hours ...
-
bioRxiv - Molecular Biology 2023Quote: ... antibodies in Protein LoBind tubes (Eppendorf). Thereafter ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... Low protein binding tubes (022431081, Eppendorf) were used for sample preparation ...
-
bioRxiv - Genomics 2022Quote: ... Digestion of proteins using a GELFREE 8100 fractionator was performed in protein low binding tubes (Eppendorf, Hamburg, Germany) using the Single-Pot Solid-Phase-enhanced Sample Preparation technique described previously (Hughes et al. ...
-
bioRxiv - Biochemistry 2021Quote: ... all samples were adjusted to the same volume and concentration (ca. 1 mg/mL) and transferred to protein low-bind microcentrifuge tubes (Eppendorf, Hamburg, Germany). To precipitate the proteins ...
-
bioRxiv - Developmental Biology 2022Quote: ... Protein was quantified on a BioSpectrometer (Eppendorf) with a Bradford Assay (Bradford Reagent-E530-1L) ...
-
bioRxiv - Biochemistry 2021Quote: ... Protein LoBind microfuge tubes (1.5 ml, Eppendorf) were used to further minimize non-specific binding to the walls of the reaction vessel ...
-
bioRxiv - Microbiology 2020Quote: ... The protein content was colorimetrically determined (Eppendorf Biophotometer ...