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Citations for Beckman :
1 - 50 of 771 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... anti-HLA-DR(Beckman Coulter, Villepinte, France). Isotype controls with irrelevant specificities were included as negative controls ...
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bioRxiv - Cell Biology 2021Quote: ... HLA-DR (BioLegend, eBiosciences, BD Biosciences, Beckman Coulter Diagnostics). For intracellular staining of FOXP3 and CTLA4 (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... _ all from Biolegend _ and HLA-DR (ECD, clone Immu-357) from Beckman Coulter ...
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bioRxiv - Bioengineering 2021Quote: ... or using flow cytometry (HEK293, CytoFlex, Beckman Coulter).
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bioRxiv - Cell Biology 2021Quote: ... HLA class I stained cells were analyzed by FACS (Beckman Coulter). Results were analyzed using Kaluza Software ...
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bioRxiv - Cell Biology 2024Quote: ... 1:20 human FITC-BRIC6 (IBGRL Research) and 1:20 human APC-anti-CD49d (Beckman Coulter). For viability analyses ...
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bioRxiv - Immunology 2022Quote: ... HLA DR/DP/DQ (BioRad clone Bu26; Beckman clone I3; BioLegend, clone Tü39), and intracellular cytokines IFN-γ (BD ...
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bioRxiv - Cell Biology 2020Quote: ... anti-CD105 and anti-HLA-ABC antibodies (suppl. Table 1) and analysed by flow cytometry (Cytoflex; Software Cytexpert 2.3, Beckman-Coulter, Krefeld, Germany). Upon passage 3 ...
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bioRxiv - Microbiology 2021Quote: ... Linear sucrose gradients were prepared by layering 1.8 ml each of sucrose solutions (wt/wt; prepared in 5 mM EDTA, pH 7.5) into 14-by 95-mm ultracentrifuge tubes (Beckman) in the following order ...
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bioRxiv - Microbiology 2020Quote: ... Particles from media were concentrated by ultracentrifugation through a 20% (wt/wt) sucrose cushion (90 min at 27,000 rpm in a Beckman SW32 rotor ...
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bioRxiv - Microbiology 2020Quote: ... followed by concentration of fixation-inactivated virions from media by ultracentrifugation through a 20% (wt/wt) sucrose cushion (120 min at 27,000 rpm in a Beckman SW32 rotor ...
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bioRxiv - Microbiology 2023Quote: ... concentration of fixation-inactivated virions from media were spun down by ultracentrifugation through a 20% (wt/wt) sucrose cushion (120 min at 27,000 rpm in a Beckman SW32Ti rotor ...
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bioRxiv - Immunology 2020Quote: ... and antibodies against membrane markers were added (CD45, CD14, HLA-DR from Beckman Coulter). Intracellular staining was performed using the IntraPrep fixation permeabilization kit (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... and 25% (wt/wt in 12.5ml) sucrose gradient in 25 mM citrate buffer in 38 ml Ultraclear tubes (Beckman-Coulter, 344058). The gradient was centrifuged at 100,000 × g for 18 h at 4°C (Beckman-Coulter XPN-80 Ultracentrifuge ...
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bioRxiv - Microbiology 2020Quote: ... Particles from media were concentrated by ultracentrifugation through a 20% (wt/wt) sucrose cushion (90 min at 27,000 rpm in a Beckman SW32 rotor; Beckman Coulter Life Sciences). Pelleted particles were resuspended in PBS and stored in aliquots at −80°C.
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bioRxiv - Microbiology 2020Quote: ... followed by concentration of fixation-inactivated virions from media by ultracentrifugation through a 20% (wt/wt) sucrose cushion (120 min at 27,000 rpm in a Beckman SW32 rotor; Beckman Coulter Life Sciences). Pelleted particles were resuspended in PBS and stored in aliquots at −80°C.
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bioRxiv - Microbiology 2023Quote: ... concentration of fixation-inactivated virions from media were spun down by ultracentrifugation through a 20% (wt/wt) sucrose cushion (120 min at 27,000 rpm in a Beckman SW32Ti rotor; Beckman Coulter Life Sciences). Pelleted particles were resuspended in PBS and stored in aliquots at −80°C.
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bioRxiv - Microbiology 2020Quote: ... CLP assembly reactions were incubated at 25°C for 30 min before loading onto 15-30% (wt/wt) sucrose gradients and spinning at 159,599 X g for 43 min at 4°C (Beckman Coulter TLS 55 rotor). Gradients were immediately fractionated and aliquots of each fraction were denatured by boiling in 1% SDS to ensure antibody access ...
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bioRxiv - Cell Biology 2020Quote: ... APC-A750 anti-human CD90 (Thy-1/310, #B36121 Beckman Coulter) and Vioblue anti-human CD45 antibodies (REA747 ...
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bioRxiv - Bioengineering 2024Quote: ... The absorption spectrum of the resulting phage-FAM complex was measured using a DU800 (Beckman) spectrophotometer.
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bioRxiv - Immunology 2022Quote: ... Transfectants were stained for viability using a Live dead blue dye followed by anti-HLA DR DP DQ antibodies (BioRad clone Bu26, Cat. #MCA2497F; Beckman clone I3 ...
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bioRxiv - Neuroscience 2021Quote: ... Labeling was done with anti-HLA-DR-PB and anti-CD16-FITC (Duraclone pre-coated tubes or separate antibodies, all from Beckman-Coulter) and anti-CD14-PEvio770 (Miltenyi ...
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bioRxiv - Cancer Biology 2021Quote: ... the Hi-C libraries were purified with AMPure XP beads (Beckman Coulter). Quantity and integrity of the Hi-C libraries was determined by Bioanalyzer profiles (Agilent Technologies).
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bioRxiv - Systems Biology 2021Quote: HeLa Kyoto and R1/E mESCs (gifts from Hyman lab, Dresden) and HEK293 (gift from Beckman lab, Berlin), Ptk2 cell lines (gift from Simons lab ...
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bioRxiv - Biochemistry 2023Quote: The oligomeric state of mKu-DNA complex were assessed by determining sedimentation velocity using Optima AUC (Beckman Coulter). DNA ...
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bioRxiv - Genetics 2022Quote: ... and the final Hi-C library was purified with AMPure XP beads (Beckman Coulter). Promoter Capture Hi-C was performed using a customdesign Agilent SureSelect system following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... and Hi-C template DNA was purified using 1x AMPure XP beads (Beckman Coulter, A63881) and stored at 4°C until library preparation ...
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bioRxiv - Immunology 2021Quote: Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter). Test sera were diluted 1:40 in growth medium and serially diluted ...
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bioRxiv - Cancer Biology 2022Quote: ... Then 200nL of 5ug/mL of MHCI complex sample were dispensed from the Echo qualified 384- well source plate (Beckman Coulter Life Sciences ...
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bioRxiv - Developmental Biology 2023Quote: The anti–human uncoupled primary antibodies used are listed: anti-human CD34 (Beckman, QBEnd/10), anti-human ACE (BB9 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The FITC fluorescence signals demonstrating the antibody-receptor complex on the cell surface were quantified by flow cytometry (CytoFLEX, Beckman Coulter). Relative expression levels of GPR1 mutants were represented according to the fluorescence signals.
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bioRxiv - Microbiology 2021Quote: ... and stained for 30min at room temperature with 1:160 dilution of PE-conjugated mouse-anti-human Ab to HIV-1 p24 and core antigens (Beckman Coulter, Fullerton, CA). Fluorescent profiles were recorded using a Guava easyCyte and InCyte software.
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bioRxiv - Immunology 2021Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
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bioRxiv - Microbiology 2022Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
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bioRxiv - Immunology 2022Quote: ... anti-human TCR Vβ1 (Beckman Coulter), anti-human CD3 and CD8 antibodies (BD Bioscience).
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bioRxiv - Immunology 2023Quote: ... The fluorescence signals demonstrating the antibody-receptor complex on the cell surface were quantified by flow cytometry (CytoFLEX, Beckman Coulter, Brea, CA).
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bioRxiv - Immunology 2022Quote: Human Vγ9Vδ2 T lymphocytes were stained with FITC-labeled anti-human TCR Vδ2 mAb (#IMMU389, Beckman Coulter) and APC-labeled anti-human CD3ε mAb (#UCHT1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Individual cell-free virions were then purified by ultracentrifugation through a 20% sucrose (wt/vol in PBS) cushion at 83,000 g in an SW41 rotor (Beckman) for 2 h min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... ECF were purified by ultracentrifugation through a 20% sucrose (wt/vol in PBS) cushion at 38,500g in an SW41 rotor (Beckman) for 1 h min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... The fluorescence signals for the antibody-receptor complex on the cell surface were then quantified by flow cytometry (CytoFLEX, Beckman Coulter, Brea, CA). Data were analyzed using FlowJo™ v10.8 Software (BD Life Sciences) ...
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bioRxiv - Microbiology 2022Quote: ... and layered onto a preformed 11 mL 5% to 15% (wt/vol) Ficoll gradient in a 13.2 mL thin-wall polyallomer ultracentrifuge tube (Beckman Coulter). Gradients were centrifuged at 12,000 rpm for 2 h at 4°C in an SW41 Ti swinging-bucket rotor in a Sorvall Discovery SE ultracentrifuge ...
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bioRxiv - Molecular Biology 2020Quote: ... The target library derived from the oocyte RNA polymerase II transcripts was PCR-amplified (15 cycles for P14 WT, 16 cycles for P14 Tbpl2−/− mutant) and purified using AMPure beads (Beckman Coulter) to remove short PCR artifacts (< 200bp ...
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bioRxiv - Bioengineering 2021Quote: ... APC-labelled anti-human CD8 (Beckman Coulter, #IM2469), APC- or PacificBlue-labelled anti-human CD3 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... anti-human CD4-FITC (clone 13B8.2, Beckman Coulter), anti-human CD8a-BV421 (clone RPA-T8 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with PBS at the indicated time and then stained for GPA/Band3/CD49d expression with 1:20 human BV421-anti-235a (Beckman Coulter), 1:20 human FITC-BRIC6 (IBGRL Research ...
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bioRxiv - Molecular Biology 2022Quote: ... Single-cell clones were then assessed for binding to His-tagged SARS-CoV-2 Spike using a Cytoflex S (Beckman) cell analyser ...
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bioRxiv - Cancer Biology 2021Quote: Proliferation of IL-32 KO and WT mock cells was assessed by cell counting every day for 4 days using cell coulter (Beckman Coulter Diagnostics). Cell viability was assessed by Trypan blue staining or by annexin/PI flow cytometry and analyzed by FACS Diva software (BD Biosciences).
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bioRxiv - Microbiology 2020Quote: ... were pelleted through a 20% (wt/vol) sucrose cushion at 25,000 rpm for 2 h in an SW41 rotor (Beckman Coulter, Tokyo, Japan). Then ...
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bioRxiv - Neuroscience 2024Quote: ... The amount of p24 capsid protein was quantified by the HIV-1 p24 antigen ELISA (Beckman Coulter). All transductions were normalized relative to p24 capsid protein quantification.
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bioRxiv - Molecular Biology 2021Quote: ... or 13 nM anti-human CD81 PE (Beckman Coulter), respectively ...