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Citations for Bio-Rad :
1 - 50 of 10000+ citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The protein concentration of exosomes was determined by using the BCA protein determination kit (BIO-RAD, 500-0201). Western blotting and flow cytometry were used to detect exosome markers ...
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bioRxiv - Immunology 2024Quote: ... 1:1200 diluted mouse anti-human HLA-E monoclonal primary antibody (clone MEM-E/02, Bio-Rad) and as secondary antibody goat anti-mouse polyclonal antibody conjugated with horse radish peroxidase (HRP ...
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bioRxiv - Cancer Biology 2022Quote: ... Human primers for HLA-DRB1 and VCAM1 were purchased from Bio-Rad.
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bioRxiv - Cancer Biology 2022Quote: ... and anti-HLA-B27 (Bio-Rad, HLA-ABC-m3). Samples were run on a Sony SH800 cell sorter.
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bioRxiv - Genomics 2023Quote: ... Citrate synthase activity was standardized against the protein concentration of each sample using the Bovine Serum Albumin Assay (500-0201, Bio-Rad Laboratories, Inc., Hercules, CA, USA) and reported as nmol min−1 mg−1 protein after comparison with a glutathione (GSH ...
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bioRxiv - Immunology 2022Quote: ... HLA DR/DP/DQ (BioRad clone Bu26 ...
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bioRxiv - Immunology 2022Quote: ... We used mouse anti-human HLA-ABC (W6/32) conjugated to Alexa Fluor® 647 (MCA81A647, Bio-Rad), which is known to also react with MHC-I of African Green monkey from which Vero E6 cells are derived(48 ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2021Quote: ... Protein concentrations for both WT and sod1Δ resuspensions were determined by DC protein assay (Bio-Rad Laboratories) and mixed equivalently generating 400 µg total.
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bioRxiv - Developmental Biology 2020Quote: ... and anti HLA-G (Bio-rad MCA2043) followed by horseradish peroxidise-conjugated secondary antibodies anti-rabbit (Bio-Rad 170–6515) ...
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... The WT SCO4122 protein was purified via passing through the column (SuperdexTM 75, Biorad) at a flow rate of 0.125ml/min and pressure of 76-77psi ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti Human Protein Gene Product 9.5 (PGP9.5) (1:500, MCA4750GA mouse monoclonal, Biorad) for 36 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... or His-tag antibody (MCA1396, Bio-Rad, 1:1000), or rabbit monoclonal β-actin antibody (D6A8 ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant His-tagged proteins were purified using a 5 mL IMAC column (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... the His-FUS proteins were mixed with Profinity IMAC Ni2+-charged resin (Bio-Rad) for 30 min at 20 °C ...
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bioRxiv - Immunology 2022Quote: ... and anti-HLA-DQ (from either Biorad or MyBioSource) were purchased from the respective providers ...
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bioRxiv - Microbiology 2023Quote: ... 15 µl of proteins complex solution were separated by 4–20% SDS-PAGE gels (mini protean TGX, BioRad), blotted onto nitrocellulose ...
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bioRxiv - Plant Biology 2020Quote: ... The His-tagged fusion protein was purified using a Ni-NTA column (Bio-Rad, USA). Protein was eluted in elution buffer [500 mMNaCl ...
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bioRxiv - Microbiology 2024Quote: ... Detection of His-tagged proteins was performed with the ClarityTM Western ECL substrate (Biorad, USA) and using a Versadoc (Biorad ...
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bioRxiv - Microbiology 2022Quote: ... mouse monoclonal His tag antibody (MCA1396GA, Bio-Rad, 1:1000), mouse 2A antibody (NBP2-59627 ...
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bioRxiv - Immunology 2023Quote: ... Colorimetric (standards) and chemiluminescent detection (His-tagged proteins) were performed using the ChemiDoc Imager (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... followed by incubation with mouse anti-His (1:1000, BioRad MCA1396GA) overnight at 4 °C and secondary antibody detection with IRDye800CW anti-mouse (1:5000 ...
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bioRxiv - Biochemistry 2022Quote: ... and the protein was passed through Hi-Q anion exchange column (Bio-Rad Laboratories, Hercules, CA, USA) to remove minor nucleic acid contamination ...
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bioRxiv - Plant Biology 2021Quote: Purified His and GST fusion proteins or GST alone (500 ng) were blotted onto nitrocellulose membrane (BioRad). The nitrocellulose membrane was rinsed with TBS-T buffer (10 mM Tris-HCl at pH 7.4 ...
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bioRxiv - Microbiology 2024Quote: ... Chelated PBS was prepared by mixing with 1% (wt/vol) Chelex-100 (BioRad), which was rotated overnight at 4°C and passed through a 0.22 µm Steriflip (MilliporeSigma ...
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bioRxiv - Molecular Biology 2024Quote: ... The His::STL-1 was purified using Nuvia IMAC column (Bio-Rad) in the presence of washing buffer (20 mM HEPES ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins in the cleared lysate were purified using Profinity™ IMAC Nickel Charged Resin (Bio-Rad) and eluted in 50 mM sodium phosphate buffer with 300 mM NaCl pH 8 and 300 mM imidazole (Fischer Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... The presence of the complex was assessed by 4-15% gradient SDS-PAGE using Mini-PROTEAN® TGX™ Precast Protein gels (BIORAD). Concentration of the RNAP in these fractions was determined by absorbance at 280 nm using NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermofisher ...
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bioRxiv - Molecular Biology 2023Quote: ... His-tagged recombinant protein was purified using Ni-NTA purification with the help of Ni-charged resins (Bio-Rad). Purified protein was further used for kinase assay ...
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bioRxiv - Genetics 2024Quote: ... The rabbit polyclonal anti-Sakura antibodies were affinity purified using His-MBP-Sakura recombinant protein and Affigel-l5 (Bio-rad), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2020Quote: ... Polyacrylamide gels (Bio-Rad, 4-16 wt%). IFNγ ELISA (DY485 ...
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bioRxiv - Genetics 2021Quote: ... 4% (wt/vol) acrylamide (Bio-Rad, USA), 0.05% (wt/vol ...
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bioRxiv - Bioengineering 2022Quote: ... Polyacrylamide gels (Bio-Rad, 4-16 wt%). IFNγ ELISA (430104 ...
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bioRxiv - Microbiology 2023Quote: ... A mouse anti-His antibody (Biorad) was used as a primary antibody in a 1:500 dilution ...
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bioRxiv - Microbiology 2022Quote: ... mouse-anti His (BIO-RAD; MCA1396GA), mouse-anti p65 (F-6 ...
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bioRxiv - Immunology 2023Quote: ... goat-anti-human 1:3000 (Biorad /1721050); goat-anti-rabbit 1:2000 (CST / 7074) ...
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bioRxiv - Cell Biology 2023Quote: ... The complex was then purified using size exclusion chromatography (BioRad, NGC QuestTM 10 Chromatography System #7880001) ...
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bioRxiv - Immunology 2023Quote: ... The samples and a protein ladder (a 1:1 mix of All Blue Prestained Protein Standards: Unstained Protein Standards; Precision Plus Protein™, Bio-Rad) were loaded to 4-15% mini-PROTEAN™ TGX Stain-Free™ Protein Gels (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... The recovery of tag-less MlaC was quantified using the ratio standard curve method (41) while that of His-tagged nanodisc-embedded complexes were quantified using the Lowry assay (DC Protein Assay, Bio-Rad). For retrograde assay ...
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bioRxiv - Immunology 2023Quote: Purified recombinant His-tagged proteins were diluted to 0.5µg/ml in either Laemmli sample buffer alone (Bio-Rad, Hercules, CA, USA) or containing 50 mM dithiothreitol (DTT ...
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bioRxiv - Microbiology 2024Quote: ... using Anti-His HRP antibody (Bio-Rad). Identical volumes were loaded to allow comparison of protein concentrations between fractions.
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bioRxiv - Cell Biology 2022Quote: Images of THP-1 wt and THP-1 RTN1A+ cells in cultures were taken with the ZOE fluorescence cell imager (Bio-Rad). and the numbers of cellular aggregates per 0.7 mm2 from 4 FOV have been quantified using ImageJ Fiji (Fig 5G) ...
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bioRxiv - Microbiology 2020Quote: ... The plates were washed and incubated with mouse anti-His (Bio-Rad MCA-1396; 1:1000) followed by horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (MerckMillipore AP124P ...
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bioRxiv - Microbiology 2023Quote: ... Binding of VHHs was detected using a mouse anti-HIS-tag antibody (Biorad, MCA1396, 1/2000) and an AF647 conjugated donkey anti mouse IgG antibody (Invitrogen ...
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bioRxiv - Plant Biology 2022Quote: ... 10% (wt/vol) chelex resin (Bio-Rad, Hercules, CA) was mixed with the beads and immunoprecipitated DNA was eluted after reverse cross-linking by boiling at 95 °C for 10 min ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatant was incubated with HIS-affinity resin (BioRad) for 20 minutes with shaking at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated for at least 16 hours at 4°C with either mouse monoclonal anti-His (1:2,000; AD1.1.10, Biorad) or mouse anti-RNA pol (1:5,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1× AP3B1-HEX-labelled human reference assay (dHsaCP1000001, BioRAD), 1/40 HindIII (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... 1× AP3B1-HEX labelled human reference assay (Bio-Rad), 1/40 HaeIII (Invitrogen ...
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bioRxiv - Physiology 2023Quote: ... the target complex was detected by ChemiDocTM Imaging System (Bio-Rad, USA). The target band intensity was quantified using the Image LabTM software (Bio-Rad ...