-
No products found
because this supplier's products are not listed.
Michel Thépaut, et al.,
bioRxiv - Immunology 2020
Quote:
... Ebola virus Makona Glycoprotein (EBOV-GP) (KM233102.1) was synthesized and cloned into pcDNA3.1 by GeneArt AG technology (Life Technologies, Regensburg, Germany) or VSV-G following the manufacturer’s instructions of Lipofectamine 3000 (Fisher Scientific) ...
-
No products found
because this supplier's products are not listed.
Xiahui Chen, et al.,
bioRxiv - Bioengineering 2021
Quote:
sGP and RBD (GenScript) protein binder selection was done according to previously established protocols15 ...
-
No products found
because this supplier's products are not listed.
Hoang Nguyen, et al.,
bioRxiv - Molecular Biology 2021
Quote:
... Vesicular Stomatitis Virus glycoprotein (VSV-G) envelope expression vector (pMD2.G; Addgene #12259), lentiviral packaging plasmid (psPax2 ...
-
No products found
because this supplier's products are not listed.
Yuting Zhang, et al.,
bioRxiv - Immunology 2023
Quote:
... Pseudotyped ebola virus were incubated at 37°C for 1 h with 200 μg/ml thermolysin (Sigma, T7902). The reaction was stopped by addition of 400 μM Phosphoramidon (Sigma ...
-
No products found
because this supplier's products are not listed.
Kristin D. Dahl, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... MOG (Myelin oligodendrocyte glycoprotein antibody, Abcam Cat# ab32760 ...
-
No products found
because this supplier's products are not listed.
Shu Liu, et al.,
bioRxiv - Neuroscience 2020
Quote:
... mutations were inserted into the open reading frame of Vesicular stomatitis virus glycoprotein VSV-G using the Q5 SDM kit (NEB). Myc epitope-tagged SARS-CoV-2 (2019-nCoV ...
-
No products found
because this supplier's products are not listed.
Edward B. Irvine, et al.,
bioRxiv - Immunology 2021
Quote:
... Zaire ebolavirus glycoprotein (R&D Systems) was used as a negative control for the attenuated Mtb analysis.
-
No products found
because this supplier's products are not listed.
Matthew H. Raymonda, et al.,
bioRxiv - Microbiology 2023
Quote:
... and 0.25 μg vesicular stomatitis virus G glycoprotein expression plasmids using the Fugene 6 transfection reagent (Promega) according to the manufacturer’s recommendations ...
-
No products found
because this supplier's products are not listed.
Germán Hernández-Alonso, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
DNA from modern wolf PZW (SGP-824) was extracted using the DNeasy Blood & Tissue kit (Qiagen) according to the manufacturer’s recommendations ...
-
No products found
because this supplier's products are not listed.
Seyedehmahsa Moghimi, et al.,
bioRxiv - Microbiology 2022
Quote:
... expressing Moloney murine leukemia virus gag and pol genes was co-transfected with pLNCX2 vector with the FLAG-APEX2-GARG1060 insert and a plasmid coding for the vesicular stomatitis virus envelope glycoprotein (Takara Bio) using Mirus 2020 DNA transfection reagent (Mirus) ...
-
No products found
because this supplier's products are not listed.
Tiong Kit Tan, et al.,
bioRxiv - Immunology 2020
Quote:
... Negative control ‘decoy’ tetramers were similarly assembled using biotinylated Nipah virus soluble glycoprotein 65 and streptavidin-PerCP Cy5.5 (BioLegend). PBMC were washed in cold PBS and seeded at 1 × 106 cells/well in 96-well round bottom plates and with combinations of RBD and decoy tetramers by incubation for 30 min on ice ...
-
No products found
because this supplier's products are not listed.
So Young Kim, et al.,
bioRxiv - Biochemistry 2020
Quote:
... and MERS-CoV SGPs were purchased from Sino Biological Inc ...
-
No products found
because this supplier's products are not listed.
Laura K. Cole, et al.,
bioRxiv - Physiology 2021
Quote:
... secreted insulin (ELISA, ALPCO), total pancreatic islet glucagon content and secreted glucagon (radioimmunoassay ...
-
No products found
because this supplier's products are not listed.
Marissa D. Acciani, et al.,
bioRxiv - Microbiology 2020
Quote:
... and glycoprotein (G) proteins (courtesy of Dr. Michael Whitt; KeraFAST) (53) ...
-
No products found
because this supplier's products are not listed.
Kaito Nagashima, et al.,
bioRxiv - Immunology 2021
Quote:
... Secreted mAbs were purified using a Protein G column (GE Healthcare) and concentrated for use in downstream assays.
-
No products found
because this supplier's products are not listed.
Hiroyuki Mori, et al.,
bioRxiv - Microbiology 2022
Quote:
... Rabbit polyclonal anti-Ebola VP30 (GTX134035) was purchased from GeneTex (Irvine, CA). Rabbit polyclonal anti-CAPG antibody (10194-1-AP ...
-
No products found
because this supplier's products are not listed.
Theofilus A. Tockary, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Secreted Embryonic Alkaline Phosphatase (SEAP) (Invivogen) were purchased for various cell processing and analysis experiments ...
-
No products found
because this supplier's products are not listed.
Rebecca P. Sumner, et al.,
bioRxiv - Immunology 2023
Quote:
... Sendai virus was from Charles River Laboratories ...
-
No products found
because this supplier's products are not listed.
Mihai Ancău, et al.,
bioRxiv - Neuroscience 2023
Quote:
... MOG (myelin oligodendrocyte glycoprotein, Cat#MAB5622, Merck Millipore) and NFH (neurofilament heavy chain ...
-
No products found
because this supplier's products are not listed.
Yan Wang, et al.,
bioRxiv - Microbiology 2021
Quote:
... pEIAV-GagPol and vesicular stomatitis virus glycoprotein (VSVG) expression plasmids using the PEI transfection reagent (Polysciences) as described previously ...
-
No products found
because this supplier's products are not listed.
Kelly Mitchell, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... and virus was concentrated with PEG-it Virus Precipitation solution (System Biosciences). SORE6-GFP virus was added to CSCs plated on Geltrex ...
-
No products found
because this supplier's products are not listed.
María Laura Kevorkian, et al.,
bioRxiv - Microbiology 2022
Quote:
... influenza virus hemagglutinin (Roche), followed by 1:4000 anti-rat horseradish peroxidase (HRP ...
-
No products found
because this supplier's products are not listed.
Biana Bernshtein, et al.,
bioRxiv - Immunology 2023
Quote:
... Tetanus toxin and Ebola (CEFTA, Mabtech Inc) were used as a control ...
-
No products found
because this supplier's products are not listed.
Kristina Lanko, et al.,
bioRxiv - Microbiology 2020
Quote:
Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ...
-
No products found
because this supplier's products are not listed.
Matthew J. Payea, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... Conditioned media was analyzed for secreted protein using a Bio-Plex 200 (BioRad) multiplex ELISA machine using analyte plates manufactured by Luminex.
-
No products found
because this supplier's products are not listed.
Kamyab Javanmardi, et al.,
bioRxiv - Biochemistry 2021
Quote:
... The plasmid expressing the VSV-G (vesicular stomatitis virus glycoprotein) was purchased from Cell Biolabs (pCMV-VSV-G, Part No. RV-110). The lentiviral plasmid used to generate the HEK-293T stable cell lines expressing the human ACE2 gene (GenBank ID NM_021804 ...
-
No products found
because this supplier's products are not listed.
YounHa Kim, et al.,
bioRxiv - Cell Biology 2022
Quote:
... and secreted TNF-α was measured using a TNF-α ELISA kit (BD Biosciences) following the manufacturer’s instructions.
-
No products found
because this supplier's products are not listed.
Alexis S. Gorin, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Virus was injected iontophoretically (Nanoject II, Drummond Scientific) via a glass micropipette (tip diameter ∼15-20 μm ...
-
No products found
because this supplier's products are not listed.
Mostafa Jarahian, et al.,
bioRxiv - Immunology 2020
Quote:
... sapiens-wt) glycoprotein from Advanced Biomart (San Gabriel, CA, USA) and the anti-Ebola surface glycoprotein [KZ52] from Absolute Antibody (Oxford, GB). The obtained EBOV-GP was expressed with a polyhistidine-tag at the C-terminus and consists of 629 amino acids ...
-
No products found
because this supplier's products are not listed.
Mateo I Sanchez, Alice Y Ting,
bioRxiv - Synthetic Biology 2019
Quote:
... the supernatant (containing secreted AAV virus) was collected and filtered through a 0.45-μm syringe filter (VWR). AAV virus was aliquoted into sterile Eppendorf tubes (0.5 mL/tube) ...
-
No products found
because this supplier's products are not listed.
Immacolata Porreca, et al.,
bioRxiv - Molecular Biology 2023
Quote:
The MultiCyt® QBeads® PlexScreen Secreted Protein Assay Kit (Sartorius) was used to quantify the level of tumor necrosis factor alpha (TNF-a ...
-
No products found
because this supplier's products are not listed.
Gloria P. Larson, et al.,
bioRxiv - Microbiology 2019
Quote:
Multicycle replication infections were performed by inoculating A549 cells at a multiplicity of infection (MOI) of 0.01 using virus diluted in virus growth media (VGM) (DMEM supplemented with penicillin/streptomycin [Corning cellgro] ...
-
No products found
because this supplier's products are not listed.
S.N. Freda, et al.,
bioRxiv - Neuroscience 2022
Quote:
... Virus was injected using a microsyringe pump (World Precision Instruments); >5 minutes elapsed before the pipette was moved from the ventral injection site to the more dorsal injection site ...
-
No products found
because this supplier's products are not listed.
Nathaniel Kastan, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Ad-RFP virus (Vector Biolabs) at the same titer were used as a control.
-
No products found
because this supplier's products are not listed.
Leonard A. Daly, et al.,
bioRxiv - Biochemistry 2023
Quote:
... Secreted proteins were captured by addition of 100:1 (v/v) secretome:Strataclean resin (Agilent Technologies) (∼2 ml ...
-
No products found
because this supplier's products are not listed.
Alessandro Bonifazi, et al.,
bioRxiv - Neuroscience 2024
Quote:
Secreted insulin was detected using a commercially available insulin detection kit (PerkinElmer/Cisbio Bioassays, Bedford, MA) based on homogenous time-resolved fluorescence resonance energy transfer (HTRF ...
-
No products found
because this supplier's products are not listed.
Pascal Brandys, et al.,
bioRxiv - Immunology 2022
Quote:
... The column was washed with PBS and the bound glycoproteins were eluted with glycoprotein eluting solution for mannose/glucose binding lectins (Vector Labs: ES-1100). The glycoproteins were dialyzed in PBS ...
-
No products found
because this supplier's products are not listed.
Michael B. Fessler, et al.,
bioRxiv - Immunology 2022
Quote:
... The virus sequence was confirmed by Illumina sequencing and did not differ from the parental virus ...
-
No products found
because this supplier's products are not listed.
Eleonora Sementino, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... Then virus particles and polybrene (sc-134220; Santa Cruz) at a concentration of 2 μg/ml were added to the plate ...
-
No products found
because this supplier's products are not listed.
Lisa Dettinger, et al.,
bioRxiv - Microbiology 2021
Quote:
... Complete rabies virus nucleoprotein and glycoprotein gene sequences were generated from rabies virus RNA extracted using Direct-zol RNA MiniPrep kit (R2052 Zymo, Irvine, CA, USA). Complete nucleoprotein and glycoprotein genes were amplified using Takara long amplicon Taq polymerase with GC buffers (RR02AG Takara Bio USA ...
-
No products found
because this supplier's products are not listed.
Xin Tong, et al.,
bioRxiv - Molecular Biology 2019
Quote:
... Secreted insulin was measured using ELISA (Mercodia); results were normalized to total DNA content ...
-
No products found
because this supplier's products are not listed.
Philip Pickford, et al.,
bioRxiv - Pharmacology and Toxicology 2019
Quote:
... and analysed for secreted insulin by HTRF (Insulin High Range Assay, Cisbio). Insulin secretion was expressed relative to that from cells stimulated with 11 mM glucose alone in the same experiment.
-
No products found
because this supplier's products are not listed.
Jamin Liu, et al.,
bioRxiv - Microbiology 2022
Quote:
... 293T cells were transfected with arenavirus glycoprotein expression plasmids using TransIT-LT1 (Mirus Bio). Cells were transduced the following day with VSV-ΔG-GFP (Kerafast)(45 ...
-
No products found
because this supplier's products are not listed.
Lok R. Joshi, et al.,
bioRxiv - Immunology 2021
Quote:
... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ...
-
No products found
because this supplier's products are not listed.
Jerry Yan, et al.,
bioRxiv - Bioengineering 2023
Quote:
... plated at 800,000 splenocytes/well and restimulated with 10 μg rabies virus glycoprotein peptide (AnaSpec) for 24h at 37 °C ...
-
No products found
because this supplier's products are not listed.
Victoria L. Corbit, et al.,
bioRxiv - Neuroscience 2020
Quote:
... Virus was injected using a syringe pump (Harvard Apparatus) fitted with a syringe (Hamilton ...
-
No products found
because this supplier's products are not listed.
James E. Dennis, Taylor Splawn, Thomas J. Kean,
bioRxiv - Cell Biology 2019
Quote:
... secreted Gaussia luciferase was custom ordered (GeneCopoeia; HIV-based lentiviral third generation ...
-
No products found
because this supplier's products are not listed.
Francisco J. Salguero, et al.,
bioRxiv - Microbiology 2020
Quote:
... nucleocapsid(N) or membrane glycoprotein (M) sequence (Miltenyi Biotec, Bisley, UK) or 5 μg/ml staphylococcal enterotoxin b (SEB ...
-
No products found
because this supplier's products are not listed.
Jenny Y. Pham, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
This study used Escherichia virus T4 (American Type Culture Collection [ATCC] #11303-B4) and three wild-type bacterial hosts ...
-
No products found
because this supplier's products are not listed.
Miranda K. Culley, et al.,
bioRxiv - Cell Biology 2022
Quote:
Secreted endothelin levels in concentrated serum-free media were assessed by colorimetric change was measured by spectrophotometer (Biotek) using the Endothelin-1 ELISA kit (Enzo Life Sciences).