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Ebola Virus Secreted Glycoprotein sGP Bundibugyo
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The Native Antigen Company Sponsored
Recombinant AntigenCat# EBOVUG2007-SGP-1000, 1mg USD $5268.0 Ask
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Thermo Fisher
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... Ebola virus Makona Glycoprotein (EBOV-GP) (KM233102.1) was synthesized and cloned into pcDNA3.1 by GeneArt AG technology (Life Technologies, Regensburg, Germany) or VSV-G following the manufacturer’s instructions of Lipofectamine 3000 (Fisher Scientific) ... -
GenScript
No products found because this supplier's products are not listed.bioRxiv - Bioengineering 2021Quote: sGP and RBD (GenScript) protein binder selection was done according to previously established protocols15 ... -
Addgene
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2021Quote: ... Vesicular Stomatitis Virus glycoprotein (VSV-G) envelope expression vector (pMD2.G; Addgene #12259), lentiviral packaging plasmid (psPax2 ... -
Millipore Sigma
No products found because this supplier's products are not listed.Cited in droplet-Tn-Seq combines microfluidics with Tn-Seq identifying complex single-cell phenotypesbioRxiv - Microbiology 2018, published in Nature Communications doi: 10.1038/s41467-019-13719-9Quote: ... human alpha-1-acid glycoprotein (Sigma - G9885), and agarose (Lonza - Seaplaque ... -
abcam
No products found because this supplier's products are not listed.Cited in A novel strategy to express different antigens from one modified vaccinia Ankara vaccine vectorbioRxiv - Immunology 2017Quote: ... added to the membrane and incubated over night at 4°C: Rabies Virus Glycoprotein antibody 1C5c (Abcam; Cat.No.# ab82460); Hepatitis B surface antigen (AVIVA Systems Biology ... -
New England Biolabs
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... mutations were inserted into the open reading frame of Vesicular stomatitis virus glycoprotein VSV-G using the Q5 SDM kit (NEB). Myc epitope-tagged SARS-CoV-2 (2019-nCoV ... -
R&D Systems
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... Zaire ebolavirus glycoprotein (R&D Systems) was used as a negative control for the attenuated Mtb analysis. -
Qiagen
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... Virus RNA virus was extracted by Qiamp viral RNA (Qiagen) and quantified using Qbit 3 Fluorometer (Thermo Fisher Scientific ... -
Promega
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018Quote: ... and pMD.G (vesicular stomatitis virus glycoprotein [VSV-G] envelope plasmid) [53] at a ratio of 1:1:0.5 using FuGENE 6 (Promega) according to the manufacturer’s protocol ... -
BioLegend
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... Negative control ‘decoy’ tetramers were similarly assembled using biotinylated Nipah virus soluble glycoprotein 65 and streptavidin-PerCP Cy5.5 (BioLegend). PBMC were washed in cold PBS and seeded at 1 × 106 cells/well in 96-well round bottom plates and with combinations of RBD and decoy tetramers by incubation for 30 min on ice ... -
GE Life Sciences
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... Secreted mAbs were purified using a Protein G column (GE Healthcare) and concentrated for use in downstream assays. -
Takara Bio
No products found because this supplier's products are not listed.bioRxiv - Cancer Biology 2020Quote: ... CA-MSCs lentivirally transduced with a secreted luciferase construct were encapsulated in alginate and the media was sampled for secreted luciferase overtime using the Ready-to-glow Secreted luciferase reporter system (Takara). -
Sino Biological
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2020Quote: ... and MERS-CoV SGPs were purchased from Sino Biological Inc ... -
Alpco Diagnostics
No products found because this supplier's products are not listed.bioRxiv - Physiology 2020Quote: ... secreted insulin (ELISA, ALPCO) and total pancreatic islet glucagon (radioimmunoassay ... -
Roche
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in mSphere doi: 10.1128/mSphere.00354-19Quote: Secreted hGH was quantified by ELISA (Roche, 11585878001). Briefly ... -
Charles River Labs
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... Rescued virus was then amplified via inoculation of virus into 10-day-old embryonated chicken eggs (Charles River) at 37 °C for three days. -
Absolute Antibody Ltd
No products found because this supplier's products are not listed.Cited in Activating NK- receptors, homing selectins and inhibitory Siglecs recognize EBOLA-GP and HPV-L1NKbioRxiv - Immunology 2020Quote: ... sapiens-wt) glycoprotein from Advanced Biomart (San Gabriel, CA, USA) and the anti-Ebola surface glycoprotein [KZ52] from Absolute Antibody (Oxford, GB). The obtained EBOV-GP was expressed with a polyhistidine-tag at the C-terminus and consists of 629 amino acids ... -
System Biosciences
No products found because this supplier's products are not listed.Cited in A rectal cancer model establishes a platform to study individual responses to chemoradiationbioRxiv - Cancer Biology 2019Quote: ... the virus media was concentrated using PEG-it Virus Precipitation Solution (SBI System Biosciences), and then the lentiviral particles resuspended in 300 µL PBS ... -
Becton, Dickinson and Company
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2022Quote: ... and secreted TNF-α was measured using a TNF-α ELISA kit (BD Biosciences) following the manufacturer’s instructions. -
Cell Biolabs
No products found because this supplier's products are not listed.bioRxiv - Biochemistry 2021Quote: ... while the plasmid expressing VSV G (vesicular stomatitis virus glycoprotein) was purchased from Cell Biolabs (pCMV-VSV-G, Part No. RV-110). The HDM-IDTSpike-fixK plasmid was employed as a template for site-directed mutagenesis to generate the expression plasmid for the D614G mutant of SARS-CoV-2 spike protein ... -
Merck
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in PLOS ONE doi: 10.1371/journal.pone.0211602Quote: ... Secreted proteins were collected after a 72-h incubation by Amicon Ultra-15 Centrifugal Filter Units (Merck) according to manufacturer’s protocol ... -
Drummond Scientific
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... and virus was injected using either a Nanoject (Drummond Scientific) and glass pulled pipette or a syringe pump (Harvard Scientific ... -
Santa Cruz
No products found because this supplier's products are not listed.Cited in A novel strategy to express different antigens from one modified vaccinia Ankara vaccine vectorbioRxiv - Immunology 2017Quote: ... Anti-rabies glycoprotein antibody RV1C5 (Santa Cruz Biotechnology; Cat.No.#sc-57995) 1:1000 ... -
Genetex
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... mouse anti-SARS-CoV-2 spike glycoprotein (1:200, GeneTex, GTX632604), rabbit anti-SARS-CoV-2 spike glycoprotein C-terminal (1:200 ... -
World Precision Instruments
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2022Quote: ... Virus was injected using a microsyringe pump (World Precision Instruments); >5 minutes elapsed before the pipette was moved from the ventral injection site to the more dorsal injection site ... -
Polysciences
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... pEIAV-GagPol and vesicular stomatitis virus glycoprotein (VSVG) expression plasmids using the PEI transfection reagent (Polysciences) as described previously ... -
Macherey-Nagel GmbH
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: Viral RNA was isolated from virus stocks with Nucleospin RNA virus (Macherey-Nagel) according to manufacturer protocol ... -
Corning
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2019, published in Cell Reports doi: 10.1016/j.celrep.2019.10.064Quote: Multicycle replication infections were performed by inoculating A549 cells at a multiplicity of infection (MOI) of 0.01 using virus diluted in virus growth media (VGM) (DMEM supplemented with penicillin/streptomycin [Corning cellgro] ... -
VWR
No products found because this supplier's products are not listed.bioRxiv - Synthetic Biology 2019, published in Nature Methods doi: 10.1038/s41592-019-0665-7Quote: ... the supernatant (containing secreted AAV virus) was collected and filtered through a 0.45-μm syringe filter (VWR). AAV virus was aliquoted into sterile Eppendorf tubes (0.5 mL/tube) ... -
Mirus Bio
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2018, published in Journal of Virology doi: 10.1128/jvi.00971-18Quote: ... and 100 ng of L-VSV-G (vesicular stomatitis virus glycoprotein) for pseudotyping in 100 μL serum-free medium with TransIT-LT1 transfection reagent (Mirus Bio). Supernatants containing virus were harvested after 48 hours and clarified through 0.2-micron filters ... -
Vector Biolabs
No products found because this supplier's products are not listed.bioRxiv - Developmental Biology 2020Quote: ... Ad-RFP virus (Vector Biolabs) at the same titer were used as a control. -
Bio-Rad
No products found because this supplier's products are not listed.Cited in Fibroblastic reticular cells provide a supportive niche for lymph node-resident macrophagesbioRxiv - Immunology 2021Quote: ... Supernatants were removed at stated time points and secreted cytokine quantities were measured using Luminex Bead technology according to Bioplex protocols and cytokine Kit (Biorad). -
Mercodia
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2019, published in Molecular Metabolism doi: 10.1016/j.molmet.2020.100975Quote: ... Secreted insulin was measured using ELISA (Mercodia); results were normalized to total DNA content ... -
Agilent
No products found because this supplier's products are not listed.bioRxiv - Cell Biology 2018Quote: Glycoprotein Ib: a mouse monoclonal antibody (Dako AS, Glostrup, Denmark). -
Beckman
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... ultra-centrifugation of virus culuture supernatant and the virus pellet in 30% sucrose cushion gradient were performed using Optima-L 100K ultracentrifuge (Beckman Coulter) at 18,000 RPM for 1.5 hours ... -
PerkinElmer
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: The quantification of HIV-1 p24 capsid protein was performed in captured virus lysates and virus-containing supernatants with the high-sensitivity AlphaLISA p24 detection kit following the manufacturer’s (PerkinElmer) instructions ... -
Mabtech
No products found because this supplier's products are not listed.bioRxiv - Immunology 2021Quote: ... IE2 and envelope glycoprotein B (Mabtech, OH) at a final concentration of 1 mg/ml for each individual peptide (Supplemental Table 2) ... -
KeraFAST
No products found because this supplier's products are not listed.bioRxiv - Immunology 2022Quote: ... G*ΔG-VSV virus (VSV G pseudotyped virus, Kerafast) is used to infect 293T cells (American Type Culture Collection [ATCC] ... -
Invivogen
No products found because this supplier's products are not listed.Cited in Role of Inflammasome-independent Activation of IL-1β by the Pseudomonas aeruginosa Protease LasBbioRxiv - Microbiology 2020Quote: ... reporter cell supernatants were analyzed for secreted alkaline phosphatase activity using HEK-Blue Detection reagent (Invivogen). Cytokines were quantified by enzyme-linked immunosorbent assay following the manufacturer’s protocol (R&D Systems) ... -
Sartorius
No products found because this supplier's products are not listed.bioRxiv - Molecular Biology 2020Quote: ... The collected virus was filtered (0.45 μm filter, Sartorius) and concentrated using PEG-it overnight at 4°C (SBI Integrated Sciences ... -
CisBio
No products found because this supplier's products are not listed.bioRxiv - Pharmacology and Toxicology 2019, published in British Journal of Pharmacology doi: 10.1111/bph.15134Quote: ... and analysed for secreted insulin by HTRF (Insulin High Range Assay, Cisbio). Insulin secretion was expressed relative to that from cells stimulated with 11 mM glucose alone in the same experiment. -
Illumina
No products found because this supplier's products are not listed.Cited in Inhibiting LSD1 suppresses coronavirus-induced inflammation but spares innate antiviral activitybioRxiv - Immunology 2021Quote: ... The identity of the virus was confirmed by Illumina sequencing (see supplementary table 1). -
Zymo Research
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2021Quote: ... Complete rabies virus nucleoprotein and glycoprotein gene sequences were generated from rabies virus RNA extracted using Direct-zol RNA MiniPrep kit (R2052 Zymo, Irvine, CA, USA). Complete nucleoprotein and glycoprotein genes were amplified using Takara long amplicon Taq polymerase with GC buffers (RR02AG Takara Bio USA ... -
Origene Technologies
No products found because this supplier's products are not listed.bioRxiv - Immunology 2020Quote: ... HEK293T cells were individually transfected with plasmids encoding for secreted proteins (OriGene Technologies ... -
Harvard Apparatus
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2020Quote: ... Virus was injected using a syringe pump (Harvard Apparatus) fitted with a syringe (Hamilton ... -
American Type Culture Collection
No products found because this supplier's products are not listed.bioRxiv - Evolutionary Biology 2019, published in MicrobiologyOpen doi: 10.1002/mbo3.968Quote: This study used Escherichia virus T4 (American Type Culture Collection [ATCC] #11303-B4) and three wild-type bacterial hosts ... -
GeneCopoeia
No products found because this supplier's products are not listed.Cited in High-throughput, temporal and dose dependent, effect of vitamins and minerals on chondrogenesisbioRxiv - Cell Biology 2019Quote: ... secreted Gaussia luciferase was custom ordered (GeneCopoeia; HIV-based lentiviral third generation ... -
Miltenyi Biotec
No products found because this supplier's products are not listed.bioRxiv - Microbiology 2020Quote: ... nucleocapsid(N) or membrane glycoprotein (M) sequence (Miltenyi Biotec, Bisley, UK) or 5 μg/ml staphylococcal enterotoxin b (SEB ... -
The Native Antigen Company
Recombinant AntigenCat# EBOVUG2007-SGP-1000, 1mg USD $5268.0 AskbioRxiv - Immunology 2021Quote: ... Spike Glycoprotein (Full-Length)-His (REC31868-500, The Native Antigen Company). Briefly ... -
Electron Microscopy Sciences
No products found because this supplier's products are not listed.bioRxiv - Neuroscience 2018, published in eLife doi: 10.7554/eLife.36768Quote: ... Virus expression confirmation was performed using a BlueStar UV light (Electron Microscopy Sciences) at three days post injection ...