Labshake search
Citations for Bio-Rad :
1 - 36 of 36 citations for Ebola Virus Secreted Glycoprotein sGP Bundibugyo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2023Quote: ... Conditioned media was analyzed for secreted protein using a Bio-Plex 200 (BioRad) multiplex ELISA machine using analyte plates manufactured by Luminex.
-
bioRxiv - Cell Biology 2023Quote: ... EVs and ENPs secreted by 20×106 cells were resuspended in Laemmli sample buffer (Bio-Rad), boiled 10 min at 95°C and run in 4% - 15% Mini-Protean TGX Stain-Free gels (Bio-Rad ...
-
bioRxiv - Molecular Biology 2023Quote: ... a 160kD glycoprotein expressed by murine macrophages 235 (1:100, Bio-Rad clone C1:A3-1, Cat. MCA497) and rabbit anti-laminin (1:50 ...
-
bioRxiv - Microbiology 2023Quote: gp120 or SOSIP glycoproteins were separated on 8–16% SDS-PAGE (mini-PROTEAN TGX protein gels; Bio-Rad) and transferred to a 0.45 μm nitrocellulose membrane (catalog number 1620115 ...
-
bioRxiv - Neuroscience 2023Quote: ... Glycoproteins were eluted by boiling at 100°C for 10 minutes using 4x Laemmli sample buffer (Bio-Rad). Proteins were separated using a 4-12% Bis-Tris Protein SDS-PAGE Gel (Life Technologies) ...
-
bioRxiv - Microbiology 2022Quote: 25 μl of the secreted proteins were ran on a Mini-PROTEAN® TGX™ precast gel (Bio-Rad). The whole lane of the gel for each sample was sliced into 4 mm sections and washed with 1 ml of 50% ethanol in 50 mM ammonium bicarbonate (ABC) ...
-
bioRxiv - Immunology 2021Quote: ... Supernatants were removed at stated time points and secreted cytokine quantities were measured using Luminex Bead technology according to Bioplex protocols and cytokine Kit (Biorad).
-
bioRxiv - Bioengineering 2022Quote: 17 cytokines secreted from the HCCoC were analyzed using Bio-Plex Pro Human Cytokine Group-I Panel (M5000031YV, BioRad, CA). HCCoCs were exposed to physiological WSS for 6 hours after completion of the preconditioning protocol ...
-
bioRxiv - Neuroscience 2020Quote: ... The concentration of each secreted inflammatory mediator was quantified with xPONENT software version 4.2 (Biorad Laboratories Inc., Hercules, CA, USA). Differences were considered statistically significant at the 0.05 level of confidence.
-
bioRxiv - Microbiology 2019Quote: ... Proteins from cellular and secreted fractions were resolved by SDS-PAGE and transferred onto a PVDF membrane by using a transfer apparatus (Bio-Rad). The membrane was probed with primary antibody against C58 VgrG1 (1:1,000) ...
-
bioRxiv - Microbiology 2021Quote: ... and anti-canine distemper virus nucleoprotein mouse monoclonal primary antibody (Biorad) in selected fox tissues.
-
bioRxiv - Neuroscience 2024Quote: ... The final virus samples were titered by digital droplet PCR (BioRad) using a REP-specific primer/probe set for libraries and a CMV primer/probe set for recombinant virus (Supplementary Table 2) ...
-
bioRxiv - Cell Biology 2020Quote: ... 9 µL of each cell lysate and 14 µL of each secreted protein sample were run on a 4–20% Tris-glycine gel (Bio-Rad; 4561094) together with the Precision Plus Protein Dual Color Standards molecular weight marker (Bio-Rad ...
-
bioRxiv - Biochemistry 2023Quote: ... The culture medium after incubation was analyzed for the presence of secreted human cytokines using Bio-Plex Pro Human Cytokine 17-plex Assay (Bio-Rad, USA) and Bio-Plex 200 Systems (Bio-Rad ...
-
bioRxiv - Biochemistry 2021Quote: ... Virus was inactivated by adding 50 μl of 2X Laemmli buffer (BioRad) and incubating at 95 °C for 10 minutes.
-
bioRxiv - Microbiology 2021Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
-
bioRxiv - Microbiology 2022Quote: Virus or pseudovirus concentrates were lysed in 4x Laemmli buffer (Bio-rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
-
bioRxiv - Microbiology 2021Quote: Concentrated PV or virus was mixed with 4x Laemmli sample buffer (Bio-Rad) with 10% β-mercaptoethanol and run on SDS-PAGE gels ...
-
bioRxiv - Immunology 2023Quote: ... (ii) genes in RNA virus infection panel of pre-designed human PrimePCR by BioRad; (iii ...
-
bioRxiv - Biochemistry 2020Quote: ... it was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad). The use of human blood was approved by the Fiocruz Ethical Committee (CAAE 53419815.9.0000.5248).
-
bioRxiv - Biochemistry 2022Quote: ... The blood was screened for Dengue virus (DENV) using the Dengue NS1 Ag STRIP (Bio-Rad) before use in the mosquitoes feeding process ...
-
bioRxiv - Immunology 2021Quote: ... and TaqMan Fast Virus 1-step Master Mix) using CFX-96 (Bio-Rad, Hercules, CA, USA).
-
bioRxiv - Microbiology 2023Quote: ... Concentrated virus was then inactivated and proteins denatured by heating with 4x Laemmli sample buffer (Bio-Rad) with 10% β-mercaptoethanol and subsequently run on a 4-20% Mini-PROTEAN TGX protein gel (Bio-Rad) ...
-
bioRxiv - Microbiology 2021Quote: Total viral RNA was extracted from 0.1 ml virus stocks using Aurum Total RNA Mini Kit (Bio-Rad) and eluted in 50 μl elution buffer pre-heated to 70°C ...
-
bioRxiv - Microbiology 2021Quote: ... All serum samples were confirmed as Dengue virus-positive by means of NS1 diagnostic ELISA test (Platelia, Biorad). Serum samples were filtered using Millipore 0.22 μm PES syringe filters ...
-
bioRxiv - Microbiology 2020Quote: Resuspended purified beta-propiolactone treated SARS-CoV-2 virus preps were separated by SDS-PAGE (Bio-Rad TGX Mini) and transferred to 0.2 µM PVDF membrane according to the manufacturer’s protocols (Bio-Rad Tansblot Turbo) ...
-
bioRxiv - Microbiology 2022Quote: ... Virus was then inactivated and whole cell lysates taken by the addition of 2× Laemmli buffer (Bio-Rad #161073) + 2-mercaptoethanol (Bio-Rad #1610710 ...
-
bioRxiv - Microbiology 2021Quote: The specific fluorescence produced during the formation of amplicons/ PCR product from the genomic RNA (converted to cDNA) of Rabies virus was identified by the detector in the qPCR machine (CFX CONNECT, BIORAD). The quantitative result is expressed in terms of relative quantification ...
-
bioRxiv - Pathology 2022Quote: ... with an in-house full virus standard for determination of genome loads on a C1000 thermal cycler with the CFX96 Real-Time System (Biorad).
-
bioRxiv - Microbiology 2020Quote: ... One microgram of each concentrated virus preparation was resolved on a 4-20% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad) and the S-F protein and the NDV hemagglutinin-neuraminidase (HN ...
-
bioRxiv - Microbiology 2022Quote: ... Absolute quantifications of virus RNA copies were estimated by modelling a Poisson distribution using QuantaSoft™ analysis software version 1.7 (Bio-Rad). Mean signal from N1 and N2 was extrapolated and expressed as viral RNA copies per microliter of supernatant ...
-
bioRxiv - Cancer Biology 2022Quote: ... Virus quantification of KO/activation pool was done by droplet digital PCR (ddPCR) using QX200 Droplet Digital PCR System (Bio-RAD, #1864001).
-
bioRxiv - Immunology 2019Quote: ... The calculation of IgG antibody concentration against each individual influenza virus strain rHA was performed by Bio-Plex Manager™ 6.2 software (Bio-Rad Co., CA).
-
bioRxiv - Systems Biology 2021Quote: Differential expression of ten selected transcripts in response to virus at 3-L1 stage was examined using Droplet Digital PCR (ddPCR) (Bio-Rad, Hercules, CA). Because of the difficulty in obtaining a large amount of gut RNA samples and the limited sensitivity of conventional qPCR technique to detect gene expression at a low level ...
-
bioRxiv - Pathology 2023Quote: Rabies virus N gene was amplified by real-time quantitative PCR using iTaq™ Universal SYBR® Green One-Step Kit (BIO-RAD). The primers are as follow ...
-
Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... RNA copy numbers were determined by ddPCR (see AAV virus quantification) using Taqman primers for the WPRE element (Sup. Table 1) and Rpp30 (Biorad, assay ID: dMmuCPE5097025) as housekeeping gene.