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Citations for Invivogen :
1 - 29 of 29 citations for Ebola Virus Secreted Glycoprotein sGP Bundibugyo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Secreted Embryonic Alkaline Phosphatase (SEAP) (Invivogen) were purchased for various cell processing and analysis experiments ...
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bioRxiv - Biochemistry 2023Quote: HEK293T cells stably expressing a secreted IFNβ-luciferase reporter (Invivogen) were transfected with indicated plasmids ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected and secreted Luciferase activity assayed using QUANTI-Luc™ kit (InVivoGen) as per manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... The secreted lucia was measured in the culture medium using QUANTI-Luc reagent (InvivoGen, France), substrate for luciferase ...
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bioRxiv - Microbiology 2020Quote: ... reporter cell supernatants were analyzed for secreted alkaline phosphatase activity using HEK-Blue Detection reagent (Invivogen). Cytokines were quantified by enzyme-linked immunosorbent assay following the manufacturer’s protocol (R&D Systems) ...
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bioRxiv - Microbiology 2023Quote: ... and TLR9 activation was quantified by measuring secreted embryonic alkaline phosphatase (SEAP) by QuantiBlueTM reagent (Invivogen) using a microplate reader (BioTek Synergy HI ...
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bioRxiv - Bioengineering 2020Quote: Nanobodies targeting RBD were expressed and secreted as Fc fusions by cloning into pFUSE-hlgG1-Fc2 (Invivogen) using the NcoI and EcoRI restriction sites or by Gibson assembly ...
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bioRxiv - Molecular Biology 2022Quote: ... Supernatant from each plate was removed and secreted luciferase was detected by adding Quanti-Luc substrate (InvivoGen) and measured on a SpectraMax iD5 plate reader (Molecular Devices ...
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bioRxiv - Microbiology 2023Quote: HEK-BlueTM hTLR2 secreted alkaline phosphatase (SEAP) reporter assays were performed according to manufacturer instructions (Invivogen, hkb-htlr2). Briefly ...
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bioRxiv - Immunology 2021Quote: ... and 10µl was quantified for secreted luciferase (Renilla) activity in the presence of luciferase substrate (Quanti-Luc; rep-qlc2, Invivogen). Luminescence was recorded on a FLUOstar OPTIMA microplate reader (BMG Labtech).
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bioRxiv - Immunology 2021Quote: Activity of the secreted Lucia luciferase under IRF3 promoter control (THP1 Dual cells) was assessed using QuantiLuc substrate (Invivogen) according to manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... were infected with either 30 μl of 500 TCID50 mouse-adapted PR8-HA-GP61-80 or 2×105 PFU of LCMV Armstrong by intranasal inoculation or immunized by intramuscular (quadriceps) injection with 2 μg LCMV recombinant glycoprotein (rGP) with addition of Addavax (InvivoGen) adjuvant at a 1:1 ratio ...
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bioRxiv - Microbiology 2021Quote: ... the human monocyte cell line THP1-Blue expressing an NF-κB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene (InvivoGen) were cultured in RPMI-1640 (Sigma Aldrich ...
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bioRxiv - Microbiology 2024Quote: HEK-Blue cells expressing human or murine TLR9 and carrying the NF-κB SEAP (secreted embryonic alkaline phosphatase) reporter gene (InvivoGen) were used according to the manufacturer’s instructions and adapted to assess TLR-specific NF-κB activity induced via live C ...
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bioRxiv - Microbiology 2024Quote: ... NF-κB activity was monitored by the production of secreted embryonic alkaline phosphatase (SEAP) through the hydrolysis of HEK-Blue (InvivoGen). Optical density was read at 650 nm using a SpectraMax340 PC plate reader (Molecular Devices).
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bioRxiv - Microbiology 2022Quote: ... and TLR9 and TLR7 activation was determined by measuring secreted embryonic alkaline phosphatase (SEAP) reporter activity in cell culture supernatants by QuantiBlue™ reagent (Invivogen) using a microplate reader (BioTek Synergy HI ...
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bioRxiv - Immunology 2022Quote: ... Cells were co-cultured for 24h and cell-cell fusion was assessed measuring secreted embryonic alkaline phosphatase (SEAP) activity into cells supernatant using QUANTI-Blue™ Solution (Invivogen), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... virus stocks were confirmed to be free of mycoplasma (PlasmoTest, InvivoGen) and other adventitious agents by deep sequencing on a MiSeq platform (Illumina ...
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bioRxiv - Immunology 2024Quote: Human TLR4 reporter cell line HEK-TLR4 cells containing a nuclear factor κ-light-chain-enhancer of activated B cells (NFκB)-inducible secreted embryonic alkaline phosphate (SEAP) reporter (InvivoGen; San Diego, CA) were used to assess the effect of resistin on TLR4 activation as previously reported15 ...
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bioRxiv - Immunology 2020Quote: ... NF-kB reporter monocytic cells (THP-1-XBlue) stably expressing a secreted embryonic alkaline phosphatase (SEAP) reporter inducible by NF-κB were purchased from InvivoGen (InvivoGen, San Diego, CA, USA). - ...
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bioRxiv - Genetics 2019Quote: ... Virus transduced cells were maintained for 2 weeks under blasticidin (10 μg/ml, Invivogen) selection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: THP1-Blue™ NF-κB cells (derived from the human THP-1 monocyte cell line by stable integration of an NF-kB-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene (InvivoGen, San Diego, CA, USA; 2 × 105 per well) were cultured in 96 well plate using RPMI containing 10 % heat inactivated FBS ...
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bioRxiv - Cell Biology 2020Quote: ... cells infected with shRNA encoding virus were selected in puromycin (2 μg/mL, InvivoGen, ant-pr) and used 4 days post infection.
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bioRxiv - Physiology 2023Quote: The selection for virus-infected HDFs has performed with 4μg/ml blasticidin (Invivogen, San Diego, California) selection media ...
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bioRxiv - Microbiology 2023Quote: ... Envelope defective virus was pseudotyped with HU-1 SARS-CoV-2 Spike protein (pLV-Spike, Invivogen) and used for single round infection assays ...
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bioRxiv - Microbiology 2021Quote: ... Virus samples were then diluted 1:1 with the adjuvant Addavax (Invivogen cat. no. vac-adx-10) to a final concentration of 100 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... Mice were infected intranasally with 3×104 PFU of x31-PmpG-1 virus and boosted intranasally with 1μg PmpG-1303-311 peptide + 2μg CpG (ODN 1826, Invivogen) on days 14 and 28 (75) ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transduced with murine leukemia virus-based transduction vectors and subsequently transduced cells were selected with puromycin (Invivogen). Authentication of cell lines was performed by STR-typing ...
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bioRxiv - Genetics 2019Quote: ... The untransduced cells and serially diluted virus-treated cells were cultured in the presence of 2 μg/ml puromycin or 20 μg/ml blasticidin S (InvivoGen). When almost all of the untransduced cells had died ...