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Citations for Thermo Fisher :
1 - 50 of 829 citations for Ebola Virus Secreted Glycoprotein sGP Bundibugyo since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Ebola virus Makona Glycoprotein (EBOV-GP) (KM233102.1) was synthesized and cloned into pcDNA3.1 by GeneArt AG technology (Life Technologies, Regensburg, Germany) or VSV-G following the manufacturer’s instructions of Lipofectamine 3000 (Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... Vesicular stomatitis virus glycoprotein-pseudotyped virus was produced by co-transfecting 293T cells using Lipofectamine 2000 (Invitrogen) with an shRNA transducing vector and 2 packaging vectors ...
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bioRxiv - Microbiology 2022Quote: ... plasmid encoding myc- and His-tagged full-length EBOV GP (Zaire ebolavirus isolate Ebola virus/H.sapiens-wt/SLE/2014/Makona-G3838) using Lipofectamine™ 3000 (Thermo Fisher Scientific Cat. Nr. L3000) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and 4 μg of plasmid VSV-G encoding G glycoprotein of vesicular stomatitis virus(VSVG) using Lipofectamine 3000 Reagent (Invitrogen). 12 h later ...
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bioRxiv - Cancer Biology 2022Quote: Glycoproteins were isolated using a glycoprotein isolation kit (89804; Thermo Fisher Scientific) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: SADS-CoV spike glycoprotein (virus strain GDS04; GenBank No.: ASK51717.1) gene was synthesized with codons optimized and inserted into pFastBac vector (Life Technologies Inc.). The ectodomain of SADS-CoV spike protein without the transmembrane anchor and intracellular tail (residues 18-1068 ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were co-transfected with the pNL4.3.Luc.R-E-viral vector as well as a plasmid expressing the vesicular stomatitis virus glycoprotein (VSV-G) envelope (pMD2.G) using Lipofectamine 3000 (Life Technologies) according to basic manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Ebola GP expression plasmid kindly provided by Jochen Bodem) using Lipofectamine™ 3000 (ThermoFisher) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... was then used for glycoprotein purification using both ConA and WGA Glycoprotein Isolation Kits (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... glycoproteins were digested with trypsin (ThermoFisher) overnight at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... and Pierce Glycoprotein Staining kit (ThermoFisher) were used for protein and glycan staining ...
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bioRxiv - Biochemistry 2021Quote: ... while the other was stained for glycoproteins using the Pro-Q Emerald 300 glycoprotein staining kit (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Pichiapink secreted protein kit (Thermofisher scientific) containing one shot electro-competent E ...
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bioRxiv - Genetics 2022Quote: ... Tissues were incubated with mouse anti-P-glycoprotein C219 antibody at 3.6μg/mL (Glycoprotein Monoclonal Antibody C219, Cat. No MA1-26528, Invitrogen) overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... A glycoprotein ladder (Candy Cane ladder; Invitrogen) served as positive control ...
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bioRxiv - Microbiology 2024Quote: ... along with a CandyCane glycoprotein ladder (Thermofisher). We additionally loaded the same two B ...
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bioRxiv - Systems Biology 2020Quote: ... and a second part was stained and analysed for glycoproteins using the Pro-Q 488 Emerald Glycoprotein staining kit (P33375, Thermo/Invitrogen), according to the manufacturers protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... or with Pierce™ Glycoprotein Staining Kit (Thermo Scientific) after separation.
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bioRxiv - Plant Biology 2022Quote: ... pastoris-produced LysM protein solution was tested using a protein polyacrylamide gel followed by CBB and glycoprotein staining with the Pierce Glycoprotein Staining Kit (Thermo Fisher Scientific, California, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Pro-Q™ Emerald 300 glycoprotein gel stain kit (Invitrogen) was used to detect the presence of sugar in Bl-Eng2 before and after deglycosylation ...
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bioRxiv - Biochemistry 2021Quote: ... and cloned into the pPICZαA secreted expression vector (ThermoFisher V19520) using EcoRI and SacII restriction sites ...
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bioRxiv - Microbiology 2022Quote: ... secreted proteins were digested with trypsin (Applied Biosystems, Waltham, MA, USA) as previously described (16) ...
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bioRxiv - Bioengineering 2023Quote: Secreted progesterone was measured using the Progesterone Competitive ELISA Kit (Invitrogen #EIAP4C21). In brief ...
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bioRxiv - Cell Biology 2023Quote: ... secreted fractions from unstimulated cells were analyzed by SimplyBlueTM SafeStain (Invitrogen, LC6060) Coomassie staining (Huang et al. ...
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bioRxiv - Neuroscience 2023Quote: Secreted APOE was measured using human APOE ELISA kit (Thermo Fisher Scientific, EHAPOE) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... BacMam 2.0 virus (Invitrogen, C10593).
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bioRxiv - Immunology 2021Quote: ... The primary antibodies used were as follows: P-glycoprotein (1:1000, Invitrogen MA1-2652), Caspase-1 (1:2000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... VSV pseudotyped with EBOV glycoprotein was grown by infecting Vero cells (Ambion, Austin, TX) and then harvesting via filtration of the supernatant through 0.4 µM filters 24-30 hours after infection ...
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bioRxiv - Neuroscience 2021Quote: ... Loss of virus was monitored by staining with anti-Sendai virus antibody (Invitrogen, cat #14649482).
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bioRxiv - Microbiology 2020Quote: ... The gel was then stained using Pro-Q Emerald 300 staining for glycoproteins kit (Invitrogen) following the procedure described by the supplier.
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bioRxiv - Biochemistry 2021Quote: ... The SARS-CoV-2 spike glycoprotein RBD was expressed in Freestyle 293 cells (ThermoFisher, MA) grown in Freestyle 293 media at 37 °C with 8% CO2 and shaking at 135 rpm in a humidified incubator ...
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bioRxiv - Biochemistry 2023Quote: ... Glycoproteins were reduced with 5 mM tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl; Thermo Scientific) and alkylated with 10 mM 2-Chloroacetamide (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... cells were transfected with 30 µg of viral glycoprotein expression plasmid using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... virus with Trypan Blue (Gibco, 1µl) was injected into the lateral ventricle using glass pipette (WPI) ...
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bioRxiv - Immunology 2022Quote: ... Virus was diluted in OptiMem (ThermoFisher) and applied apically at a MOI 0.1 for two hours at 34,5°C with 5% CO2.
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bioRxiv - Bioengineering 2023Quote: ... Varicella zoster virus solution (Fisher Scientific) was dropped onto a No.1 coverslip and dried on top ...
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bioRxiv - Cell Biology 2023Quote: Cytotune OSKM Sendai virus (A16517, Invitrogen) and EmGFP reporter Sendai Virus (A16519 ...
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bioRxiv - Immunology 2021Quote: ... Secreted IL-1β was measured with the Human IL-1β cytoset ELISA (Invitrogen; Cat.# CHC1213) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... and dimeric secreted protein was purified by Protein A affinity chromatography (PierceTM #20334, Thermo Fisher). Eluted material was characterized and further purified by size-exclusion chromatography (SEC ...
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bioRxiv - Neuroscience 2020Quote: Relative quantitation of secreted Aβ40 was performed with commercial human Aβ40 ELISA kit (Invitrogen, #KHB3481) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Granzymes A and B secreted into the cell media were measured by ELISA (Abcam, Invitrogen). IELs were analyzed for CD107a externalization through flow cytometry ...
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bioRxiv - Immunology 2021Quote: ... spike glycoproteins were produced in Expi293F cells grown in suspension using Expi293 expression medium (Life Technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
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bioRxiv - Immunology 2021Quote: ... Virus was titrated by adding serial dilutions of virus supernatant (8 replicates) on VeroE6 cells in DMEM (Gibco) containing 2% FBS ...
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bioRxiv - Microbiology 2023Quote: ... Mice were anesthetized with ketamine-xylazine and infected intranasally with 5,000 PFU of the non-recombinant virus or 1,000 PFU of the recombinant virus in 50 μl of Dulbecco’s Modified Eagle Medium (DMEM) (Gibco). For antibody treatment ...
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bioRxiv - Immunology 2024Quote: ... Infection was initiated by adding 100 µl of virus inoculum containing 100.000 PFU (MOI ∼0.5-1) in virus absorption medium (Gibco OptiPro Serum free medium + 1:100 Glutamax + 10 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... The secreted S-2P and S-6P proteins were purified using HisPurTM cobalt resins (Thermo Scientific) and StrepTactin resins (IBA) ...
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bioRxiv - Immunology 2022Quote: ... The secreted fusion protein was purified on a CaptureSelect™ human albumin affinity matrix (Life Technologies), and protein eluted by adding 20 mM Tris and 2.0 M MgCl2 ...
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bioRxiv - Immunology 2020Quote: ... secreted protein was captured from High Five supernatants using HisPur Ni-NTA resin (Thermo Scientific, #88223) affinity chromatography ...
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bioRxiv - Immunology 2021Quote: Serum secreted protein measurements were obtained using a custom Luminex ProcartaPlex Multiplex kit (Thermo Fisher Scientific) to detect the following 39 analytes ...
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bioRxiv - Immunology 2024Quote: ... Proteins were expressed as secreted protein by transient transfection of HEK Expi293 cells (Thermo Fisher Scientific) and purified via CaptureSelect™ C-tag affinity matrix (Thermo Fisher Scientific) ...