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GSK-3β inhibitor
Sold for research purposes only.
Cat# 2010.0, SKU# 2010-10 mg,
10mg, US $99.00 / EA, EURO, €90 / EA
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Isabel R.K. Kuebler, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... The MCH receptor antagonist 6-(4-Chloro-phenyl)-3-[3-methoxy-4-(2-pyrrolidin-1-yl-ethoxy)-phenyl]-3H-thieno[3,2-d]pyrimidin-4-one (GW803430) (Axon Medchem, Groningen, Netherlands) was freshly prepared the day of the treatment ...
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No products found
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Nicholas A. W. Bell, et al.,
bioRxiv - Biophysics 2020
Quote:
... of λ-DNA using the following primers: 5’-CGAACTCTTCAAATTCTTCTTCCA-3’ and 5’-GATTGCTCTTCTGTAAGGTTTTG-3’ with a 5:1 ratio of dTTP:biotin-11-dUTP (Jena Bioscience). Similarly ...
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No products found
because this supplier's products are not listed.
Lihong Wang-Bishop, et al.,
bioRxiv - Bioengineering 2023
Quote:
... Mice in groups receiving αPD-1 and αCTLA-4 (100 µg, every 3 days for 5 injections, BioXcell, West Lebanon NH) were treated intraperitoneally ...
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No products found
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Etienne Lelièvre, et al.,
bioRxiv - Developmental Biology 2022
Quote:
... engMutfwd 5’-AGAACAGACGAATCTACAGCCGAA-3’ and engintron2rev 5’-AGCATGTTTTAACAAGACGGCAG-3’ primers and HotGoldStar PCR mix (Eurogentec).
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No products found
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Anna L. Vlasits, et al.,
bioRxiv - Neuroscience 2024
Quote:
... Boroscillate pipettes (Sutter Instruments, 3-5 MΩ) were used for all recordings and whole-cell electrophysiology recordings were performed using a Multiclamp 700B amplifier (Molecular devices) ...
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No products found
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Neil Fleck, Christoph Grundner,
bioRxiv - Genetics 2021
Quote:
... sequences flanked by overhangs for Gibson Assembly matching the vector insertion site (5’-CCGCATGCTTAATTAAGAAGGAGATATACAT-3’) – array sequence – (5’-GACTACAAGGATGACGACGACAAG-3’) were synthesized (Genscript) and obtained in a pUC57-Kan vector ...
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No products found
because this supplier's products are not listed.
Rotem Ben-Tov Perry, et al.,
bioRxiv - Molecular Biology 2022
Quote:
8 weeks-old C57BL/6J male mice (Envigo, Israel, n =3 per group) received bilateral stereotaxic injections of AAV9 Silc1 ...
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No products found
because this supplier's products are not listed.
Marc Duque, et al.,
bioRxiv - Neuroscience 2021
Quote:
... performing half media changes every 3-4 days with fresh NbActiv1 supplemented with PrimocinTM (InvivoGen ant-pm-1). Neurons infected with GCaMP6f as stated above were infected with AAV9-hSyn-Cre (Addgene #105553-AAV9 ...
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No products found
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Md Gulam Musawwir Khan, et al.,
bioRxiv - Cancer Biology 2021
Quote:
... Cell survival was measured using the WST-8 (water-soluble Tetrazolium-8: 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)- 5- (2,4-disulfophenyl)-2H-tetrazolium) assay kit (CCK-8; Dojindo Molecular Technologies, #CK04) following manufacturer’s instructions ...
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No products found
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Luke A Johnson, et al.,
bioRxiv - Neuroscience 2020
Quote:
... In all patients a directional “1-3-3-1” electrode was used (4/5 patients: Abbott Infinity model 6172 ...
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No products found
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Vikram Jakkamsetti, et al.,
bioRxiv - Neuroscience 2023
Quote:
We used a standard rotarod (rod diameter 3 cm, fall height 20 cm, start speed 4 rpm, acceleration rate 1 rpm/ 8 s, Touchscreen RotaRod, Harvard Apparatus) in several consecutive testing trials performed over 7 days while blinded to genotype and simultaneously testing control littermate mice ...
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No products found
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Charles R. Heller, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 1 - 4 tungsten micro-electrodes (FHC, 1-5 MΩ) were inserted to characterize the tuning and response latency of the region of cortex ...
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No products found
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Tammy H. Wharton, et al.,
bioRxiv - Molecular Biology 2023
Quote:
... and 5 X 1 minute disruption in Mini-Beadbeater-8 (Biospec Products) at 4° ...
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No products found
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Rukesh Chinthapatla, et al.,
bioRxiv - Biochemistry 2022
Quote:
... Cytidine 5’-O-(1-thiotriphosphate) and 3’-deoxycytidine 5’-triphosphate were from TriLink. Adenosine 5’-O-(1-thiotriphosphate ...
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No products found
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J. Christopher Rounds, et al.,
bioRxiv - Neuroscience 2021
Quote:
... sonicated for 3×5 minutes in a 4°C Bioruptor ultrasonicator (UCD-200, Diagenode), vortexed ...
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Cat# HY-W050145,
inquire
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Zhou Zhou, et al.,
bioRxiv - Immunology 2023
Quote:
... a group treated with the sEH inhibitor 1-(1-propanoylpiperidin-4-yl)-3-[4-(trifluoromethoxy) phenyl]urea (TPPU) (MedChemExpress LLC, 3 mg/kg per day), a group treated with CVB3 (2×106 p.f.u ...
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Prepared to contain higher collagenase and caseinase activities. A dialyzed, lyophilized powder.
Cat# LS005282,
1 gm, $246.00
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Kwangdeok Shin, et al.,
bioRxiv - Developmental Biology 2024
Quote:
... Zebrafish ventricles were fixed in 3% PFA for 5 min and incubated in PBS supplemented with 1 mg/mL collagenase type 4 (Worthington Biochemical) at 4°C overnight ...
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No products found
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Swati Gupta, et al.,
bioRxiv - Neuroscience 2020
Quote:
... and Western Blotting samples were denatured at 94°C for 5 minutes in 3 x SDS sample buffer (150mM Tris pH 8, 6% SDS, 0.3M DTT ...
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No products found
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Lin Di, et al.,
bioRxiv - Genomics 2022
Quote:
... The cell suspension was further incubated with biotinylated 4-Hydroxy-3-iodo-5-nitrophenylacetyl (NIP)15-BSA (Biosearch Technologies, N-1027-5) for 1.5h ...
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No products found
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Antonio Marino, et al.,
bioRxiv - Systems Biology 2023
Quote:
... 4 3 2.0 mm SecurityGuard (Phenomenex) cartridge as a guard column ...
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No products found
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Ru Jia, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... 5 mM Na3VO4) and 3∼4 mL of 0.5-mm diameter glass beads using FastPrep-24 (MP Biomedicals). Proteins were immunoprecipitated with GFP-Trap A beads (Chromoteck ...
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No products found
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Tirumalasetty Muni Chandra Babu, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... MTT (3-[4, 5- dimethyl thiazol-2-yl]-2,5 diphenyl tetrazolium bromide) (Alfa Aesar Chemicals Co. Ltd. Shanghai, China), fetal bovine serum (FBS ...
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No products found
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Agata Szuba, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 5 mM dithiothreitol (DTT)) at 4°C overnight and concentrated to ~3-5 mg/mL using Vivaspin 6 concentrators (Sartorius, 6 ml, 100 kDa cut-off). Mammalian septin hexamers composed of mouse Sept2 (98.6% identical to human Sept2 ...
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No products found
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Pattama Wiriyasermkul, et al.,
bioRxiv - Biochemistry 2023
Quote:
... 50 μL of 1 mg/mL XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide) (Biotium) was mixed with 5 μL of 1.5 mg/mL phenazine methosulfate ...
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No products found
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Asya Smirnov, et al.,
bioRxiv - Microbiology 2022
Quote:
... The pilT gene and flanking sequence from the FA1090 chromosome was amplified by PCR with primers PilT-F 5’-CATTGAGGTCGGCAAGCAGC-3’ and PilT-R 5’-GCATCTTTACCCAGCGCGAAAT-3’ and cloned into pSMART HC Kan (Lucigen). The cloning mix was transformed into TOP10 E ...
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No products found
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Julianna L. Sun, et al.,
bioRxiv - Microbiology 2022
Quote:
... Membranes were developed using 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) (Sigma Aldrich, 11697471001. Images were captured using a Nikon Z1000 microscope.
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No products found
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Wenjian Lv, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Cells were passaged (1:12) every 3-4 days using StemMACS Passaging Solution XF (Miltenyi Biotec) and Y-27632 dihydrochloride (Santa Cruz ...
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No products found
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Sara Lago, et al.,
bioRxiv - Cancer Biology 2024
Quote:
... pRPA32 1:1000 (Bethyl, A300-246A-8) and 53BP1 1:100 (Millipore ...
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No products found
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Yisrael Rappaport, et al.,
bioRxiv - Cell Biology 2020
Quote:
... rabbit anti-HIM-8 (Novus Biological, 1:2000), rat anti-HIM-8 (1:100 ...
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No products found
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Jennifer S. Chen, et al.,
bioRxiv - Microbiology 2020
Quote:
... and 3 dpi by high content imaging (BioTek Cytation 5) configured with brightfield and GFP cubes ...
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No products found
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Els F Halff, et al.,
bioRxiv - Neuroscience 2020
Quote:
... followed by overnight incubation at 4°C with primary antibody diluted in blocking solution (1:1000 Rabbit-α-SV2A, Abcam ab32942, Cambridge, UK; 1:300 Mouse-α-Neuroligin1/2/3/4, Synaptic Systems 129211, Göttingen, Germany). Sections were then washed in PBS (3×10 min ...
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No products found
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Stefano Cattaneo, et al.,
bioRxiv - Neuroscience 2019
Quote:
All drugs were obtained from Sigma except 2,3-dioxo-6-nitro-1,2,3,4-tetrahydrobenzo[f]quinoxaline-7-sulfonamide disodium salt (NBQX) and 2-(3-carboxypropyl)-3-amino-6-(4 methoxyphenyl)pyridazinium bromide (gabazine) which were obtained from Hello Bio (Bristol, UK).
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The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell...
Cat# B34304, SKU# B34304-25 mL (2500 rxns),
25 mL (2500 rxns), $201.00
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Kristina A.M. Arendt, et al.,
bioRxiv - Cancer Biology 2020
Quote:
In vitro cell proliferation was determined using WST-8 [water soluble tetrazolium-8 or 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulphophenyl)-2H-teterazolium] assay (Bimake; Munich, Germany). For this ...
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No products found
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Maria João Ferreira, et al.,
bioRxiv - Plant Biology 2023
Quote:
... 1 mg/mL X-Gluc (5-bromo-4-chloro-3-indolyl β-D-glucuronic cyclohexylammonium salt; Biosynth)] ON at 37°C ...
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No products found
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Amoldeep S. Kainth, Hesheng Zhang, David S. Gross,
bioRxiv - Genetics 2024
Quote:
... then 1-NM-PP1 (4-amino-1-tert-butyl-3-(1’-naphthylmethyl)pyrazolo[3-4-d]pyrimidine) (Toronto Research Chemicals, Inc.; no. A603003) was added to a final concentration of 15 μM ...
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No products found
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Zhi Zhou, et al.,
bioRxiv - Neuroscience 2023
Quote:
... Fluo-8 (AAT Bioquest; final 5 μM), Probenecid (final 1 mM) ...
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No products found
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Mariano Nicolás Di Guilmi, et al.,
bioRxiv - Neuroscience 2021
Quote:
... with a high-pass Bessel (8-pole) filter with a −3 dB cutoff of 5 Hz using Clampfit 10.6 (Molecular Devices). All data was analyzed using NeuroMatic in Igor Pro software (WaveMetrics ...
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No products found
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Tori Tonn, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... glass slides (4’’ by 3’’; Ted Pella) and the feature-side of the PDMS slabs were thoroughly cleaned with tape to remove any dust particles ...
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No products found
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Yan Chen, et al.,
bioRxiv - Molecular Biology 2022
Quote:
The purified complex samples (3 μL at 5-8 mg/mL) were applied to glow-discharged holey grids (Quantifoil R1.2/1.3, 300 mesh) and subsequently vitrified using a Vitrobot Mark IV (ThermoFisher ...
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No products found
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Morgan E. Mouchka, et al.,
bioRxiv - Evolutionary Biology 2019
Quote:
... Colonies were screened on LB plates with 50 μg/mL ampicillin and 9.5 mg/mL X-gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside; APExBIO) and Sanger sequencing of the J was used to confirm cloning and successful transformation of the ancestral λ sequence.
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No products found
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Kathryn P. Trogden, et al.,
bioRxiv - Cell Biology 2020
Quote:
... 5-8 islets per 10 mm glass bottomed dish (Mattek) were placed in the center of the glass in 100 ul RPMI 1640 and allowed to settle for 1.5-2 hours before being transferred to 5% CO2 at 37°C ...
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No products found
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Nina R. Montoya, et al.,
bioRxiv - Microbiology 2022
Quote:
... The plates were developed with TMB (3, 3’, 5, 5’ - Tetramethylbenzidine) ELISA Peroxidase Substrate (Rockland Immunochemical, Limerick, PA), and absorbance was measured on a plate reader at 655 nm.
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8 Well Chambered Cover Glass with #1 cover glass (0.13-0.16mm), with lid, sterilized. Designed...
Cat# C8-1-N,
48/case, $219.00
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Lissenya B. Argueta, et al.,
bioRxiv - Cell Biology 2021
Quote:
... hESC-qualified)-coated plates at 4×10^5/well in 24-well plates or 3×10^4/well in glass-like polymer bottom 96-well plates (CellVis).
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No products found
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Hao Li, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 3% triton X-100 (4 min, Solarbio, Beijing, China), and goat serum (15 min ...
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No products found
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Annelot C. M. van Esbroeck, et al.,
bioRxiv - Molecular Biology 2024
Quote:
... After rinsing the membrane with TBS-T (3 × 5 min) and TBS (3 × 5 min) fluorescence was detected by scanning on the Odyssey CLx (LI-COR Biosciences).
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No products found
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Walden Li, Ryan M. Wyllie, Paul A. Jensen,
bioRxiv - Microbiology 2020
Quote:
... Strains carrying the pLacZ plasmid appear blue when plated on 0.008% X-gal (5-bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Zymo Research, CA, USA). X-gal concentrations above 0.008% appear to inhibit growth of S ...
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No products found
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Stefan Coassin, et al.,
bioRxiv - Genetics 2019
Quote:
... Binding reactions for super shift assays were performed for 30 min at 4-8°C using the Binding Buffer B-1 (Active Motif 37480) together with Stabilizing Solution D (Active Motif 37488 ...
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No products found
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He-Chin Hsieh, et al.,
bioRxiv - Microbiology 2023
Quote:
... and BA.4/5 (Genetex, Cat. No. GTX137098-pro) were mixed with RBD-LTA ...
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No products found
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Rachael Gordon, et al.,
bioRxiv - Immunology 2019
Quote:
... were detected with bromo-4-chloro-3-indolyl phosphate substrate (Southern Biotech).
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No products found
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Hannah Walters, Lynne S Cox,
bioRxiv - Cell Biology 2020
Quote:
... Cells were seeded at 4-8×103 cells/cm2 in filter-capped flasks or multi-well plates (Greiner) and incubated with complete media in a humidified incubator at 37oC in 5% CO2 and 20% O2 ...