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Citations for Diagenode :
1 - 50 of 452 citations for 8 4 ethoxy 3 methoxyphenyl 1 5 diazabicyclo 3.2.1 octane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... sonicated for 3×5 minutes in a 4°C Bioruptor ultrasonicator (UCD-200, Diagenode), vortexed ...
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bioRxiv - Microbiology 2021Quote: ... 4 µg of chromatin were immunoprecipitated overnight at 4°C with 8 µg of antibody against H3K9me3 (Diagenode, C15410193) previously coupled to protein A/G magnetic beads provided in the kit ...
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bioRxiv - Microbiology 2020Quote: ... and sonication bath (3×10 sec at 4°C) (Bioruptor, Diagenode). Lysates were centrifuged (14.000x g ...
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bioRxiv - Genomics 2020Quote: ... 5 μg of purified DNA was sheared at 8 kb using the megaruptor1 system (Diagenode), followed by a DNA damage repair step on 2 μg of sample ...
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bioRxiv - Genomics 2021Quote: ... Labs 1 and 3 used Antibody 1 (Diagenode, Denville ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were sonicated (settings: 3 – 4 x 30 sec on /30 sec off, 4°C, power high; Bioruptor, Diagenode) and lysates were cleared by centrifugation at 16,000 x g at 4°C for 15 min ...
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bioRxiv - Microbiology 2021Quote: 60,000 iATII cells or Calu-3 cells were incubated for 1 h at 4°C with equal protein concentrations of control rabbit IgG (Diagenode, Cat#C15410206) or 1/200 dilution of rabbit anti-ACE2 (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... 4 × 5 min cycle (30 s on/ 30 s off; Diagenode Bioruptor) with 4 min incubation on ice between sonication cycles ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 mM TEAB and 1 x Halt protease inhibitor cocktail) and sonication (3 × 5 min cycles of 30s on/off; Diagenode Bioruptor sonication device). Extracted proteins protein were digested to peptides with in-solution digestion performed at the Turku Proteomics Facility ...
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bioRxiv - Plant Biology 2019Quote: ... For 5-mC immunolocalization slides were first incubated over night at 4°C with a monoclonal anti-5-mC-antibody (Diagenode C15200003 ...
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bioRxiv - Molecular Biology 2024Quote: ... for 5 min at 4°C and lysates were sonicated (1x 5 min, 30 sec on/off) with a Bioruptor (Diagenode) followed by digestion with 40U RNaseI (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref ...
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bioRxiv - Microbiology 2019Quote: ... washed cell pellets were lysed in 50 mM HEPES pH 8 with 5% SDS followed by 10 min (30 sec on/off) sonication in a Bioruptor Pico sonicator (Diagenode) at 18 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... then the sample was sonicated at a high intensity (3 × 5 min) using a Bioruptor sonicator (Diagenode). To obtain organoid-MPF cocultures ...
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bioRxiv - Genomics 2021Quote: ... After sonication (15 sec on/ 30 sec off, 5 cycles, 4 C) using Bioruptor 300 (Diagenode) 5 µl were stained with DAPI to verify isolation of intact Macs ...
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bioRxiv - Molecular Biology 2022Quote: ... After sonication for 5 cycles of 30 seconds ON/OFF at 4 °C (Bioruptor Pico, Diagenode), the mixture was centrifuged (13 000xg ...
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bioRxiv - Molecular Biology 2021Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Immunology 2021Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 × g ...
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bioRxiv - Immunology 2020Quote: ... sonicated at 4°C with 3 cycles of 30 s on 30 s off in a Bioruptor Pico (Diagenode) and clarified by centrifugation at 12,000 xg ...
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bioRxiv - Molecular Biology 2024Quote: ... using 3 to 4 cycles of 15 sec on : 30 sec off at low intensity in a Bioruptor (Diagenode). After recovery of the NEXSON-isolated nuclei by centrifugation (1000g ...
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bioRxiv - Genomics 2022Quote: ... the nuclei were briefly sonicated for 5 seconds at 4°C in a Bioruptor Pico sonicator (Diagenode). Permeabilized nuclei were incubated for 10 minutes at 37°C in a thermoshaker ...
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bioRxiv - Microbiology 2021Quote: ... anti-Histone 4 rabbit (1:2,000; Diagenode Cat. No C15410156-50). HRP-conjugated anti-mouse and anti-rabbit antibodies were used (1:5,000 ...
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bioRxiv - Biochemistry 2020Quote: ... After incubation at 95C for 5 min the sample was sonicated 3 times for 30 sec on/off with a Bioruptor (Diagenode) on high power ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... the remaining pellet was resuspended in 50μl RIPA supplemented with 1x Protease Inhibitor Cocktail and sonicated for 3-5 min with Picoruptor® (Diagenode). 10μg of proteins were then separated through precast 4-12% NuPAGE® Bis-Tris gels (Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... The lysates were sonicated (3 × 10 s bursts with 5 s rest) on ice at low amplitude in a Bioruptor (Diagenode) and then centrifuged at 20,817 × g for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 2mM EDTA) and briefly sonicated to remove flagella (ON 5 sec – OFF 30 sec x 3 Cycles, bioruptor Pico, Diagenode). The samples were transferred in low retention tubes ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mg of chromatin lysate was rotated overnight at 4°C with 5 μg antibody: rabbit IgG (Diagenode #C15410206), anti-NIPBL (NIPBL#1 ...
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bioRxiv - Microbiology 2023Quote: ... 40 mM 2-Chloroacetamide, 50 mM HEPES pH 8.5), boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). For the intracellular proteomes ...
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bioRxiv - Cancer Biology 2023Quote: ... 4-10 μg of gDNA were either fragmented to a size of 10-50kb with the Megaruptor 3 (Diagenode, E07010001 and E07010003) or fragmented to a size of 15-25kb with a gTUBE (Covaris ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were split into 150 μl aliquots in 0.5 ml Eppendorf tubes and sonicated at 4°C for 30 cycles (30 seconds on/off) using a Bioruptor (Diagenode). Aliquots of the same samples were pooled and spun down at 12,000xg for 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... were sonicated with 5 cycles of 30 s on followed by 30 s off at 4°C (Bioruptor Plus, Diagenode). After the centrifugation for 15 min at 4°C and 16,100 ×g ...
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bioRxiv - Microbiology 2023Quote: ... was dissolved in 75 µL lysis buffer, boiled (5 min, 95 °C) and sonicated (5x60 s, 4 °C) (Bioruptor, Diagenode). Lysates from the secretomes and intracellular fractions were centrifuged (10 min ...
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bioRxiv - Systems Biology 2021Quote: ... 750 U/µl Sm DNAse) and sonicated (5 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). HA or Streptactin beads were added to cleared lysates and samples were incubated for 3 hours at 4°C under constant rotation ...
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bioRxiv - Systems Biology 2021Quote: ... 750 U/µl Sm DNAse) and sonicated (5 min, 4°C, 30 sec on, 30 sec off, low settings; Bioruptor, Diagenode SA). Following normalization of protein concentrations of cleared lysates ...
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bioRxiv - Neuroscience 2023Quote: ... RNA samples were incubated overnight at 4°C with 12.5 µg of anti-5-hmC antibody (Diagenode rat monoclonal MAb-633HMC) or without antibody as negative control in IP buffer (750 mM NaCl ...
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bioRxiv - Physiology 2020Quote: ... Resulting nuclei were centrifuged at 12,000 RPM and resuspended in shearing buffer (1% SDS, 10mM EDTA pH 8, 50mM Tris pH8) and sheared in 1.5ml Bioruptor Microtubes (Diagenode, C30010016) in a Bioruptor (Diagenode ...
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bioRxiv - Cancer Biology 2022Quote: ... Upon 8 cycles of sonication with Pico Bioruptor (Diagenode) (30sec ON/ 30sec OFF) ...
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bioRxiv - Cell Biology 2023Quote: ... Immunostaining was performed overnight at +4°C with primary antibodies (H3K4me2 1:1000; Abcam ab32356) (H3K4me3 1:200; Diagenode C15410003) (H3K4me2 1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... CTCF ChIP-seq samples were prepared with 3 x 106 cells and 1 µg CTCF antibody (Diagenode C15410210). ChIP-seq Dilution Buffer (16.7 mM Tris-HCl pH 8.1 ...
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bioRxiv - Genomics 2020Quote: ... Genomic DNA was sheared to 8 kb fragments using Megaruptor 2 (Diagenode). Standard SMRTbell libraries were prepared using Template Preparation Kit 1.0 (Pacific Biosciences ...
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bioRxiv - Genomics 2020Quote: Preparation of reagents: ChIP Wash buffers 1 to 4 (from iDeal ChIP-seq kit for histones, Diagenode C01010173) are aliquoted into quarter module reservoirs divided by length ...
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bioRxiv - Neuroscience 2024Quote: ... The nuclear pellets were lysed with lysis buffer (50 mM Tris-HCl, 10 mM EDTA, 1% SDS, pH 8) and then sonicated with Bioruptor (Diagenode, 15 cycles, 30s ON/30s OFF). The size of the DNA in the sheared chromatin fragments was checked before precipitation by a Tapestation system (Agilent 4200 ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were sonicated with a Bioruptor Pico sonicator (Diagenode, total time 8 min) and 4μg of antibodies were added to sheared chromatin (from 4 million cells for Olig2 and from 1 million cells for histone marks ...
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bioRxiv - Cancer Biology 2022Quote: ... 1mM EDTA) and subjected to 8 cycles of sonication with Pico Bioruptor (Diagenode) (30sec ON/ 30sec OFF) ...
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Checkpoint adaptation in repair-deficient cells drives aneuploidy and resistance to genotoxic agentsbioRxiv - Cell Biology 2019Quote: ... Sonification was performed for 10 sec at level 1 using “constant” mode with a 3 mm sonification tip (Branson) or in a Bioruptor Pico (Diagenode) for 2 cycles 30 sec each ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 min total (Diagenode).
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bioRxiv - Neuroscience 2023Quote: ... Overnight incubation with primary antibodies at 4°C was performed using the following antibodies: Cas9 (Diagenode, C15200230-100, 1:500), alpha synuclein (Abcam ...
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bioRxiv - Genomics 2023Quote: ... Preclearing 30μl of Dynabeads protein A/G for 1 h at 4 °C was followed by incubation with antibodies (H3K27ac, Diagenode, 3C15410196 ...
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bioRxiv - Cell Biology 2022Quote: ... 3 μL of antibody per IP (Cas9 – Diagenode C15310258 ...