-
No products found
because this supplier's products are not listed.
Valentina Salvi, et al.,
bioRxiv - Immunology 2021
Quote:
... and PE-conjugated anti-IL-4 (clone 7A3-3, Miltenyi Biotec) following the manufacturer’s recommendations ...
-
No products found
because this supplier's products are not listed.
Michael Jakob Pichler, et al.,
bioRxiv - Microbiology 2020
Quote:
... and sonication bath (3×10 sec at 4°C) (Bioruptor, Diagenode). Lysates were centrifuged (14.000x g ...
-
No products found
because this supplier's products are not listed.
Jiashu Xu, et al.,
bioRxiv - Biophysics 2023
Quote:
... 3 μl of medium containing one isolated muscle fibre was placed on the glow-discharged grid (Quantifoil R2/1 Au 200 mesh). After incubation for 60 seconds at 37 °C ...
-
No products found
because this supplier's products are not listed.
Jian-You Lin, et al.,
bioRxiv - Neuroscience 2021
Quote:
... one of 4 gustatory stimuli (0.1M NaCl, 0.3M Sucrose ...
-
No products found
because this supplier's products are not listed.
Tori Tonn, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... glass slides (4’’ by 3’’; Ted Pella) and the feature-side of the PDMS slabs were thoroughly cleaned with tape to remove any dust particles ...
-
Prepared to contain lower tryptic activity levels to limit damage to membrane proteins and...
Cat# LS004191,
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Mengjie Zhu, et al.,
bioRxiv - Genetics 2021
Quote:
Left hind legs of P7 mice were isolated from one WT and one Col12a1-/- mouse and digested in 4 mg/mL collagenase II (Worthington, USA) in DMEM/F-12 (supplied with 10% FBS ...
-
No products found
because this supplier's products are not listed.
Anna Balcerak, et al.,
bioRxiv - Molecular Biology 2022
Quote:
... as a blocking buffer and then overnight at 4°C in the same blocking solution containing one of the following antibodies: anti-HAX1 (rabbit, Proteintech 11266-1-AP), anti-RPL26 (rabbit ...
-
No products found
because this supplier's products are not listed.
Nicholas B. Karabin, et al.,
bioRxiv - Bioengineering 2020
Quote:
... 1 µg of each protein sample was separated in one dimension using 4-20% tris-glycine gels (Mini-PROTEAN TGX, Bio-Rad Laboratories, Inc.). A 1:6 dilution of the PageRuler Plus pre-stained protein ladder (10 µl ...
-
No products found
because this supplier's products are not listed.
Alison Moss, et al.,
bioRxiv - Neuroscience 2020
Quote:
... one of the five tracers was injected into Sprague Dawley rats (3-4 months old, purchased from Envigo) sino-atrial node at the same volume of 10 μL and the heart tissues were harvested in the time window 10 am-12 pm 14 days after injection and stored in OCT immediately ...
-
No products found
because this supplier's products are not listed.
Rachel A. Silverstein, et al.,
bioRxiv - Genetics 2022
Quote:
... cerevisiae temperature-sensitive strain TSA64 en masse using EZ Kit Yeast Transformation kit (Zymo Research). The entire transformed library was grown in selective media (YPD + clonNAT ...
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No products found
because this supplier's products are not listed.
Emily J. Talbot, et al.,
bioRxiv - Cell Biology 2023
Quote:
... 4 µM digitonin) with or without 7 µM 2’,3’-cGAMP (Invivogen). Cells were further washed in PBS and incubated in culture medium until collection ...
-
No products found
because this supplier's products are not listed.
Charline Ogier, et al.,
bioRxiv - Cancer Biology 2023
Quote:
... anti-TIM3 Ab (4 μg/ml, clone RMT-3-23, BioXCell, Lebanon, NH), or 25-hydroxycholesterol (4 μM ...
-
No products found
because this supplier's products are not listed.
Kari L. Price, et al.,
bioRxiv - Developmental Biology 2020
Quote:
... Worm lysates from one freeze-thaw cycle were homogenized with a 4:100 mix of β-mercaptoethanol (MP Biomedicals) and sample buffer (NuPAGE LDS 4X Sample Buffer ...
-
No products found
because this supplier's products are not listed.
Christoph Metzendorf, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... were homogenized in liquid nitrogen pre-cooled 4 ml PTFE-vials with one 8 mm stainless steel bead using a Micro-Dismembranator (Sartorius AG, Göttingen) at 2600 RPM for two minutes ...
-
No products found
because this supplier's products are not listed.
Natalie J Haywood, et al.,
bioRxiv - Cell Biology 2021
Quote:
... Arteries were then mounted en face on slides using DAPI (DAPI-Fluoromount-G, Southern Biotech) to define nuclei ...
-
No products found
because this supplier's products are not listed.
Adam S. Lowet, et al.,
bioRxiv - Neuroscience 2024
Quote:
... and DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate, Biotium, 60014-10mg) were dissolved in 100% ethanol (Koptec ...
-
No products found
because this supplier's products are not listed.
Marco Schade, et al.,
bioRxiv - Paleontology 2022
Quote:
... 2 & 3 (Figs. 1–3; Supplementary Figs. 1–4) was performed using a Metrotom 1500 (Carl Zeiss Microscopy GmbH, Jena, Germany) in a subsidiary of Zeiss in Essingen ...
-
No products found
because this supplier's products are not listed.
Gemma L. Pearson, et al.,
bioRxiv - Cell Biology 2022
Quote:
... Sections were then incubated in the following primary antisera overnight at 4°C in 1 X PBS + 1% tween + 20% Blocking One solution: rabbit anti-Aldh1a3 (Novus Biologicals; Littleton, CO, USA). Sections were then washed twice with PBS and incubated for 2 h at room temperature with species specific Cy2 and Cy3 conjugated secondary antibodies ...
-
No products found
because this supplier's products are not listed.
Amador Romero-Franco, et al.,
bioRxiv - Cell Biology 2024
Quote:
... membranes were blotted with the appropriate primary antibody (Suppl. Table 3) and infrared dyed secondary antibodies (Suppl. Table 4) diluted in Odyssey Blocking Buffer (LI-COR) supplemented with 0.1% Tween-20 and 0.01% Sodium Azide ...
-
No products found
because this supplier's products are not listed.
Hui Yan, et al.,
bioRxiv - Immunology 2020
Quote:
... with 100 μg of NP-CGG (in average 16 molecules of NP, 4-hydroxy-3-nitrophenyl acetyl, conjugated to one molecule of CGG, chicken γ-globulin; Biosearch Technologies) in the presence of 100 μl of alum (Imject® Alum adjuvant ...
-
No products found
because this supplier's products are not listed.
Takashi Handa, Qing Zhang, Hidenori Aizawa,
bioRxiv - Neuroscience 2024
Quote:
... scopolamine (22 mM, 3-4 µl, Tokyo chemical industry). After the first drug condition ...
-
No products found
because this supplier's products are not listed.
Yeon Soo Kim, et al.,
bioRxiv - Cancer Biology 2019
Quote:
... shSIRT3_#4: 5’-TCACATTCTGTTGACTCTCCATACTCAGC-3’) in pRS vector (Origene, TR309432) were used to stably transfect OVCA433 cells (Supp ...
-
No products found
because this supplier's products are not listed.
Yoann G. Santin, et al.,
bioRxiv - Microbiology 2023
Quote:
... manually back blotted for 3-4 secondes and flash-frozen in liquid ethane using a CP-3 plunger (Gatan). Data were collected on a 300-kV CRYO ARM™ 300 (JEM-Z300FSC ...
-
No products found
because this supplier's products are not listed.
Joel Johnson George, et al.,
bioRxiv - Developmental Biology 2021
Quote:
... X-gal (5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside, Goldbio) was dissolved in dimethylformamide at 50 mg/ml ...
-
No products found
because this supplier's products are not listed.
Sergio Galindo-Trigo, et al.,
bioRxiv - Genetics 2019
Quote:
... Immunoprecipitations were performed in the same buffer with 0.5% IGEPAL for 3-4 hours at 4 °C with GFP-trap resin (Chromotek). Beads were washed 3 times with the same buffer and bound proteins were eluted by addition of SDS loading dye and heating to 90°C for 10 min ...
-
No products found
because this supplier's products are not listed.
Rosalba Perrone, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... in 3-5% Bovine Serum Albumin - Fraction V in-1X TBS for one hour (Rockland, Baltimore, MD, BSA-1000). Block was removed and slides were incubated in the respective primary antibody diluted in protein block solution for 1 hour or overnight in a humidified chamber ...
-
No products found
because this supplier's products are not listed.
S.M. Kamel, et al.,
bioRxiv - Cell Biology 2020
Quote:
... Pipettes (resistance 3–4 MΩ) were pulled from borosilicate glass capillaries (Harvard Apparatus, UK) using a custom-made microelectrode puller ...
-
No products found
because this supplier's products are not listed.
Luis M. Rivera-Perez, et al.,
bioRxiv - Neuroscience 2021
Quote:
... Slices were incubated overnight at 4°C in rabbit anti-VAChT (3:500, Synaptic Systems, cat# 139103, RRID: AB_887864). This antibody was previously validated by Western blot and has been successfully used to identify cholinergic terminals in the cochlear nucleus and hippocampus (Gillet et al. ...
-
No products found
because this supplier's products are not listed.
Lucas E. Cabrera Zapata, et al.,
bioRxiv - Neuroscience 2022
Quote:
... ChIP reactions were carried out overnight at 4 °C with 3 µg of sheared chromatin and 10 μg of anti-H3K27me3 rabbit polyclonal antibody (39155, Active Motif) or anti-mouse IgG as negative control (115-001-003 ...
-
No products found
because this supplier's products are not listed.
Elena Dvoretskova, et al.,
bioRxiv - Developmental Biology 2023
Quote:
... Following digestion with BstXI (Jena Bioscience, EN-E2118) to remove the sucrose gene ...
-
No products found
because this supplier's products are not listed.
Hao Li, et al.,
bioRxiv - Neuroscience 2020
Quote:
... 3% triton X-100 (4 min, Solarbio, Beijing, China), and goat serum (15 min ...
-
No products found
because this supplier's products are not listed.
Luke A Johnson, et al.,
bioRxiv - Neuroscience 2020
Quote:
... In all patients a directional “1-3-3-1” electrode was used (4/5 patients: Abbott Infinity model 6172 ...
-
No products found
because this supplier's products are not listed.
Ana S Almeida, et al.,
bioRxiv - Microbiology 2021
Quote:
... and three 3–4 mm sterile glass beads (Biospec Products). Next ...
-
Cat# HY-113259,
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Panagiotis Papoutsoglou, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... The SMARCA2/4 bromodomain inhibitor PFI-3 (MedChemExpress, HY-12409) and the allosteric dual SMARCA2 and brahma related gene 1 (BRG1)/SMARCA4 ATPase activity inhibitor BRM/BRG1 ATP Inhibitor-1 (MedChemExpress ...
-
No products found
because this supplier's products are not listed.
Claudia I. Semprich, et al.,
bioRxiv - Developmental Biology 2019
Quote:
... and one half the litter exposed MEK inhibitor (PD184532, MEKi, 3 μM final concentration, Enzo Life Sciences) and the other half exposed to vehicle control DMSO (equal volume ...
-
No products found
because this supplier's products are not listed.
Hao Guo, et al.,
bioRxiv - Bioengineering 2022
Quote:
... Germany) with a guard column (EC 4/3 NUCLEODUR C18 Gravity, 3 μm, Macherey-Nagel GmbH & Co. KG Germany), while the column temperature was kept at 40 °C in a thermostatic column compartment (Thermo Fisher UltiMate 3000) ...
-
No products found
because this supplier's products are not listed.
Peiqi Zhang, et al.,
bioRxiv - Microbiology 2023
Quote:
Random mutant libraries of En-Cren bacteria were generated by electroporation with the EZ-Tn5 Tnp Transposome Kit (Lucigen) and stored in 20% glycerol at -80 °C for future use in the fungal hyphae movement mutant screening ...
-
No products found
because this supplier's products are not listed.
Robert Rauschkolb, et al.,
bioRxiv - Evolutionary Biology 2023
Quote:
... from Mediterranean and temperate species had germinated we transplanted up to 20 seedlings each into 9 × 9 cm pots (one plant per pot) with a 3:1 mixture of potting soil (Einheitserde®, BioLine, Topfsubstrat Öko torffrei) and sand (0-2 mm play sand ...
-
No products found
because this supplier's products are not listed.
Chunyou Mao, et al.,
bioRxiv - Molecular Biology 2020
Quote:
... The membrane was solubilized for 3 hours at 4 °C with 0.5% (w/v) lauryl maltose neopentyl glycol (LMNG, Anatrace), 0.1% (w/v ...
-
No products found
because this supplier's products are not listed.
Brianna E. Alexander, Huaning Zhao, Sophie Astrof,
bioRxiv - Developmental Biology 2023
Quote:
... and Cyanine 3 (Akoya Bioscience, NEL744001KT) according to manufacturer protocols ...
-
Glucocorticoid modulator
Sold for research purposes only.
Cat# 1176.0, SKU# 1176-10 mg,
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Isabel R.K. Kuebler, et al.,
bioRxiv - Pharmacology and Toxicology 2023
Quote:
... The MCH receptor antagonist 6-(4-Chloro-phenyl)-3-[3-methoxy-4-(2-pyrrolidin-1-yl-ethoxy)-phenyl]-3H-thieno[3,2-d]pyrimidin-4-one (GW803430) (Axon Medchem, Groningen, Netherlands) was freshly prepared the day of the treatment ...
-
No products found
because this supplier's products are not listed.
Camille Mazo, et al.,
bioRxiv - Neuroscience 2022
Quote:
... An array of 4 tungsten electrodes (∼3 MΩ; FHC) was glued together and was slowly lowered into the OB ...
-
No products found
because this supplier's products are not listed.
HJ Monzo, et al.,
bioRxiv - Cancer Biology 2022
Quote:
... sections were blocked with 3% BSA in PBS for 30 min and incubated with SSEA-4 antibody (GTX48037, Genetex) overnight ...
-
No products found
because this supplier's products are not listed.
Ryann M. Fame, et al.,
bioRxiv - Neuroscience 2023
Quote:
... 5–10 μL of CSF or serum was extracted in 4:6:3 chloroform:methanol:water mixture supplemented with isotopically labeled T3 and T4 (at 100 nM, Cambridge Isotope Laboratories ...
-
No products found
because this supplier's products are not listed.
Devin Dersh, et al.,
bioRxiv - Cancer Biology 2020
Quote:
... were transfected at a 4:3:1 ratio using TransIT293 reagent into T225 flasks as indicated by the manufacturer (Mirus Bio). For individual sgRNA preparations ...
-
No products found
because this supplier's products are not listed.
Anastasia Accoti, et al.,
bioRxiv - Microbiology 2023
Quote:
... re-suspended in water was injected into the thorax of cold-anesthetized 3-4 day old female mosquitoes using a Nanoject III (Drummond Scientific).
-
No products found
because this supplier's products are not listed.
Mehran Najibi, et al.,
bioRxiv - Immunology 2019
Quote:
... TMB One Component HRP Microwell Substrate (Bethyl Laboratories, E102) was added ...
-
No products found
because this supplier's products are not listed.
Mariya B Shapiro, et al.,
bioRxiv - Immunology 2023
Quote:
One alpaca was immunized with PSMA-His protein (Sino Biological) and CFA/IFA adjuvant administered every 21 days over the study period ...
-
No products found
because this supplier's products are not listed.
Donatas Repecka, et al.,
bioRxiv - Synthetic Biology 2019
Quote:
... and MultiQuant 3 (Sciex) was used for analysis and quantitation of results ...
-
No products found
because this supplier's products are not listed.
Yekaterina Shulgina, et al.,
bioRxiv - Synthetic Biology 2024
Quote:
... and luminescence was measured for one hour in a Spark Plate Reader (Tecan) set to 37 °C ...