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Citations for Active Motif :
1 - 50 of 88 citations for 7α 12α Dihydroxycholest 4 en 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... ChIP reactions were carried out overnight at 4 °C with 3 µg of sheared chromatin and 10 μg of anti-H3K27me3 rabbit polyclonal antibody (39155, Active Motif) or anti-mouse IgG as negative control (115-001-003 ...
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Identification of a PRC2 accessory subunit required for subtelomeric H3K27 methylation in NeurosporabioRxiv - Molecular Biology 2020Quote: H3K27me2/3 ChIP using anti-H3K27me2/3 antibody (Active Motif, 39536) was performed as previously described (12 ...
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bioRxiv - Genetics 2021Quote: H3K27me2/3 ChIP using α-H3K27me2/3 antibody (Active Motif, 39536), which recognizes di- or trimethylated H3K27 ...
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bioRxiv - Developmental Biology 2019Quote: ... MEIS1/2/3 (Active Motif 39796) and LTL (Vectorshield FL-1321) ...
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bioRxiv - Cell Biology 2022Quote: ... Histone 3 (H3) (Active Motif 39763), Histone H3K4me3 (Active Motif 39160) ...
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bioRxiv - Developmental Biology 2020Quote: ... H3K27me2/3 (Active motif, 39535, 1:100) and EZH2(BD43 clone ...
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bioRxiv - Biophysics 2022Quote: ... and 3 μM Sortase A5 (Active Motif) in 20 mM Tris ...
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bioRxiv - Cancer Biology 2022Quote: ... (Histone 3 (Active Motif, cat no. 39763); GAPDH (Santa Cruz Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: One μg of histone H3 peptides with or without H3K4me2 (a.a. 1-21, Active Motif) were incubated with 1 μg of purified GST-PHF21A protein fragments in binding buffer (50 mM Tris/Cl at pH 7.5 ...
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bioRxiv - Biophysics 2022Quote: H3K27ac (Active Motif, 39133 Lot 06921014 – Figure 3), biological replicates from Supplementary Figure SI-Fig ...
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bioRxiv - Immunology 2019Quote: ... trimethylated H3 lysine 4 (Active Motif 39159), and anti-glutathione-S-transferase (GST ...
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bioRxiv - Cancer Biology 2022Quote: ... 4 µg H3K27ac antibody (Active Motif, 39133) was coupled to 40 µl Dynabeads M280 Anti Rabbit Magnetic beads (Thermo Fisher ...
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bioRxiv - Cell Biology 2023Quote: 3’UTR reporter vectors were purchased from Active Motif, along with the miR-4787 mimic ...
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bioRxiv - Genomics 2023Quote: ... Human Negative Control Primer Set 3 (Active Motif, #71023). For analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... MARK3 and 14-3-3ε were purchased (Active Motif, 81209 ...
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bioRxiv - Genomics 2022Quote: ... histones were extracted according to the recommended manufacturer’s one-step protocol using the Histone Extraction Kit (Active Motif). The protein concentration of the lysates was determined using the BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2020Quote: ... 3 μl of H3K27ac monoclonal antibody (Cat #39685, Active Motif) was added to each sample and rotated overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: RelA and IRF-3 transcription factor activation assays (Active Motif) were carried out using the same protocol with the only difference being the 96-well plates and the antibodies used ...
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bioRxiv - Genomics 2023Quote: ... were pre-coated with antibodies for 4 hours at 4°C and the following antibodies were used: H3K27ac (39685 – Active Motif); H3K9ac (C5B11-9549 – Cell Signalling Technology) ...
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bioRxiv - Developmental Biology 2022Quote: ... One negative control primer pair was used (Unt12, Human negative control primer set 1, Active Motif, catalog number 71001) as well as one positive control (DPF1 for RUNX2 ...
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bioRxiv - Developmental Biology 2020Quote: ... Beads were then resuspended in Blocking buffer and coated with antibodies for 4 h at 4°C (H4K20me1 [0.5 μg/IP], Active Motif cat. 39727). Once coated beads were magnet-separated and resuspended in 1 mL of lysate ...
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bioRxiv - Cancer Biology 2019Quote: ... or 4 μg of H3K27Ac antibody (Active Motif #39133). Libraries were prepared by Active Motif and sequenced on a NextSeq 500 to a depth of ∼50 M reads (Jefferson University Genomics Laboratory ...
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bioRxiv - Molecular Biology 2019Quote: ... -H3K27 di-and tri-methyl (H3K27me2/3, Active Motif, cat # 39538), -nuclear pore glycoprotein p62 (NUP62 ...
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bioRxiv - Cell Biology 2022Quote: ... 3 µl of anti-H3K4me3 antibodies (Active Motif, cat. No 39159) were added to the sonicated ChIP reaction and rotated vertically at 4° C overnight ...
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bioRxiv - Genomics 2021Quote: ... The DNA was denatured for 10 min at 95°C and immunoprecipitated overnight at 4°C with 4 μL of 5hmC monoclonal antibody (Active Motif Cat No 55010). To allow selective enrichment of immune-captured DNA fragments ...
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bioRxiv - Cancer Biology 2020Quote: SDHD 3’UTR LightSwitch Luciferase Reporter Assay was purchased from Active Motif. Cells were co-transfected with 30 nM miRNA and 100 ng reporter using Lipofectamine 3000 in a 96-well plate ...
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bioRxiv - Genomics 2021Quote: ... Chromatin was sonicated for 3 minutes with a probe sonicator (Active Motif). Finally ...
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bioRxiv - Developmental Biology 2023Quote: ... mouse anti-MEIS1/2/3 (0.5 µg/ml, 39795, Active Motif, RRID:AB_2750570), rabbit anti-DHRS3 (1:500 ...
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bioRxiv - Genomics 2023Quote: Site-specific mutagenesis was done on CCL2 LightSwitch 3′ UTR reporter (Active Motif) to generate constructs containing rs13900 C or rs13900 T alleles ...
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bioRxiv - Cancer Biology 2019Quote: ... overnight at 4 °C or H3K27ac (Active motif; Catalog no: 39133). The purified ChiP DNA was used to generate sequencing libraries using Hapa Hyper prep kit from Kapa Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... Histone deacetylases were inhibited with Romidepsin (4 nM; Active Motif, # 14083) or Panobinostat (20 nM ...
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bioRxiv - Molecular Biology 2024Quote: ... Antibody (4 μl per sample) against H3K27ac (Active Motif, cat# 39133) was conjugated to magnetic beads (Sera-Mag ...
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bioRxiv - Developmental Biology 2019Quote: ... Samples were then washed 3 times with working Washing Solution (ChIP-IT, Active Motif) and then snap-frozen in liquid nitrogen and stored at −80°C until further processing ...
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bioRxiv - Molecular Biology 2019Quote: ... and 2 µl of appropriate antibody (H3K27me2/3, Active Motif 39536, or GFP, MBL 598) was added and incubated with rotation at 4 °C overnight ...
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bioRxiv - Microbiology 2023Quote: ... samples were incubated with 3 µg of rabbit anti-H3K9ac antibody (Active Motif; Carlsbad, CA) overnight at 4°C with rocking ...
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bioRxiv - Molecular Biology 2021Quote: ... samples were incubated with 4 µg of Pol II antibody (Active Motif, 4H8) at 4°C overnight ...
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bioRxiv - Developmental Biology 2020Quote: ... then incubated 3 min with Stop Solution from the ChIP-IT High Sensitivity Kit (Active Motif) and washed three times with PBS before being frozen at −80°C until further use ...
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bioRxiv - Bioengineering 2023Quote: ... and mouse anti-MEIS 1/2/3 antibody (1:200, clone 9.2.7, 39796, Active Motif, RRID:AB_2750570). Secondary antibodies (raised in donkey ...
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bioRxiv - Neuroscience 2023Quote: ... they were incubated overnight at 4°C with the primary antibody (5mC, Active Motif, cat.no ...
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bioRxiv - Cell Biology 2020Quote: Histones were purified from cells by growing macrophages to a density of 3×107 (Active Motif - 40025). Histone H3K27me3 conversion was measured using the Active Motif kit (Epiquik Histone Methyltransferase activity assay kit (P-3005).
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bioRxiv - Developmental Biology 2019Quote: ... followed by 3 minutes incubation with Stop Solution from the ChIP-IT High sensitive kit (Active Motif). Samples were then washed 3 times with working Washing Solution (ChIP-IT ...
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bioRxiv - Systems Biology 2020Quote: ... Extracts were incubated overnight at 4°C with 5 μg of anti-H3K27ac (Active Motif) prebound to protein G Dynabeads (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies (Histone H3 – Abcam ab1791, H3k27me3 – Active Motif – 39155 and TLR-4 -Abcam 76B357.1) were then diluted 1:1000 in blocking buffer and were incubated on a rocker overnight at 4C ...
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bioRxiv - Cancer Biology 2022Quote: ... and rotated at 4°C overnight with 2-5 ug of antibody (H3K27ac, Active Motif Cat #39133 ...
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bioRxiv - Cancer Biology 2023Quote: ... and rotation at 4°C overnight with 2 ug of antibody (H3K27ac, #39133, Active Motif). Transcription factor ChIP-Seq was performed as previously described11 with 2-5 ug of antibody was used per immunoprecipitation (POU2F2 ...
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bioRxiv - Genomics 2020Quote: ... and immunoprecipitation was performed using 4 μg of the appropriate antibody (anti-CTCF: 61311, Active Motif; anti-RAD21 ...
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bioRxiv - Systems Biology 2019Quote: ... Permeabilized cells were incubated overnight at 4°C with 5ug of anti-H3K27me3 (Active Motif 39156) and then washed before incubating with protein A-MNase fusion protein (a gift from S ...
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bioRxiv - Neuroscience 2022Quote: ... washed 3 times with cold PBS and resuspended in ChIP buffer supplemented with PIC (Protease Inhibitor Cocktail; Active Motif) and PMSF (phenylmethylsulfonyl fluoride ...
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bioRxiv - Cancer Biology 2023Quote: ... After 1 hour blocking (3% donkey serum, 1% BSA, 0.3% Triton X-100, PBS) the H3.3K27M antibody (1:500, 61803, Active Motif) diluted in a blocking solution was added and incubated for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... full length of circASH1L was subcloned to the 3′UTR region of pLightSwitch backbone (Active Motif, La Hulpe, Belgium). For the PLS-cASH1L-mut plasmid ...