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Citations for GenScript :
5351 - 5400 of 6194 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... The His-tagged GMCSF peptide was quantified using a His Tag ELISA Detection Kit (GenScript) according to the provided protocol and 5-10 fold dilutions of frozen samples.
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bioRxiv - Immunology 2020Quote: ... the complete open reading frame of murine HMGB1 was cloned into pcDNA3.1(+)-C-6His vector (GenScript). Transfection was performed using FectoPRO transfection reagent (Polyplus transfection) ...
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bioRxiv - Cell Biology 2020Quote: ... followed by 10 amino acid sequences covering the four reported binding sites on the clathrin terminal domain (see Fig. 7A) were synthesized by GenScript (Piscataway, NJ) with > 95% purity ...
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bioRxiv - Immunology 2020Quote: ... BMDCs (105 cells) were incubated overnight in the presence of 10 μg/ml of influenza HA518–526 (IYSTVASSL) peptide (Genscript). IMALs were isolated from treated mice and A205804 was added to the plate for 5 d ...
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bioRxiv - Genetics 2020Quote: ... Haplotype and engineered variants of HvHKT1;5 were synthesised by GenScript (Piscataway, NJ, USA) and fragments were inserted into a gateway enabled pGEMHE vector ...
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bioRxiv - Synthetic Biology 2020Quote: ... the fxpk gene from Bifidobacterium adolescentis9,12 was synthesized (Genscript, USA) with codon-optimization for C ...
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bioRxiv - Immunology 2020Quote: ... and IgA antibodies in the rhesus serum standard: anti-S1 RBD (Genscript #HC2001), anti-S2 (SinoBiologicals #40590-D001 ...
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bioRxiv - Immunology 2020Quote: ... anti-S2 (SinoBiologicals #40590-D001) and anti-NC (Genscript #HC2003). Human and rhesus IgG antibodies were both detected in these calibration assays using biotinylated affinity-purified goat anti-human IgG γ chain polyclonal antibody (SBA #2048-08) ...
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bioRxiv - Microbiology 2020Quote: ... stable GFP expression by GAS was created by synthesizing the ribosomal binding site (RBS) and gfp gene from pDCerm-GFP (Ly et al., 2014) into the pUC57 plasmid (GenScript), resulting in pUC57-RBSGFP plasmid ...
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bioRxiv - Biochemistry 2020Quote: ... and Western blot analysis using anti-His (A01620, Genscript) and anti-PSA-NCAM (MAB5324 ...
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bioRxiv - Microbiology 2020Quote: ... coli lysate (Genscript) at final concentration of 10 mg/mL to block anti-E ...
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bioRxiv - Developmental Biology 2020Quote: The mito-APEX2_p2A_APEX2-H2B_p2A_mCherry cassette was synthesized on a kanamycin resistant pUC19 backbone (GenScript) and assembled using Multisite Gateway Cloning (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... were designed and ordered from Genscript (Piscataway, NJ, USA). The fragments matched the native 10/84 cas9 coding sequence except for the two missense mutations ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 50 μl of 0.25 μg/ml primary rabbit anti-SNAP antibody (GenScript, USA) was added and incubated for 1 h at RT ...
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bioRxiv - Pathology 2020Quote: ... a 13-mer pS33-βcat blocking phosphopeptide (QSYLDpSGIHSGAT, Genscript, Piscataway, NJ) was added to anti-βcat and anti-pS33-βcat antibodies (1:200) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The yeast THI4 coding sequence (ScTHI4) was synthesized by GenScript (Piscataway, NJ), and PCR amplified using primers 1 and 2 ...
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bioRxiv - Synthetic Biology 2020Quote: ... CCT isoforms were PCR amplified from synthetic vectors from GenScript. SpCas9 was PCR amplified from px4591 ...
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bioRxiv - Synthetic Biology 2020Quote: ... coli plasmid expression vectors (GenScript, N-terminal 8× His-tagged followed by a TEV cleavage site) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... we used custom polyclonal antibodies raised against recombinant fragment antigens generated by rabbits’ immunization (GenScript, Piscataway Township, NJ, USA). Each recombinant fragment was injected into three rabbits ...
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bioRxiv - Genomics 2020Quote: ... Designed probes were purchased as part of an oligo pool from GenScript, and the probes were amplified using a T7/Reverse-Transcriptase amplification protocol previously published,30 in an RNase-free environment with the following adaptations ...
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bioRxiv - Synthetic Biology 2020Quote: DNA sequences encoding proteins with 6xHis tags were codon-optimized by Genscript and cloned into pET28b+ or pET29b+ vector under the control of a T7 promoter ...
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bioRxiv - Synthetic Biology 2020Quote: ... The bioluminescence detection signal was measured for each design lucCage at 20 nM mixed with lucKey at 20 nM in the presence or absence of 100 nM cardiac Troponin I (Genscript, Cat. No. Z03320-50). Subsequently ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: The SARS-CoV-2 sp12 gene was codon optimized and cloned into pFastBac with C-terminal additions of a TEV site and strep tag (Genscript). The pFastBac plasmid and DH10Bac E ...
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bioRxiv - Pathology 2020Quote: ... and flanking AscI restriction enzyme sites added in the pUC57 vector (GenScript, USA). These pUC57 vectors carrying the knock-in gene of interest were then single-pot cloned (with AscI ...
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bioRxiv - Microbiology 2020Quote: ... and then incubated with 45 μL each of 0.1 μg/mL of THE anti-his-HRP (GenScript, A00612) in PBST/BSA for 1 hr at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 60 or 100 nM of his/FLAG-tagged SARS-CoV-2 spike protein (GenScript, Z03481-100) was added to each well ...
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bioRxiv - Microbiology 2020Quote: ... Anti-His-HRP (Genscript, A00612), Avidin-HRP (Biolegend ...
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bioRxiv - Microbiology 2020Quote: ... and SARS-CoV-2 spike protein (ECD, His & Flag Tag) (GenScript Z03481). Proteins were biotinylated using EZ-Link™ Sulfo-NHS-Biotin ...
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bioRxiv - Cell Biology 2020Quote: ... Bound protein was eluted using an excess of FLAG peptide (Genscript, China). The eluate was concentrated using Sartorius spin columns with a cutoff of 30kDa (Sartorius) ...
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bioRxiv - Immunology 2020Quote: Purified 5D5 Fab and CSP 81-98 peptide (GenScript) were mixed in a 1:5 molar ratio ...
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bioRxiv - Microbiology 2020Quote: ... variable genes were optimized for human cell expression and synthesized by GenScript. VH and VL were inserted separately into plasmids (gWiz or pcDNA3.4 ...
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bioRxiv - Plant Biology 2020Quote: ... NbACRE75 and NbACRE180 coding sequences were chemically synthesized by GenScript. For SlACRE75 ...
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bioRxiv - Molecular Biology 2020Quote: ... The pcDNA3.1-FLAG-FNIP1 and the pcDNA3.1+N-eGFP-FLCNΔE510 vectors were generated by Genscript. The pIRES2-FLCN plasmids were kindly provided by Dr ...
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bioRxiv - Molecular Biology 2020Quote: ... All mutations were generated by Genscript. The pcDNA3.1-FLAG-FNIP1 and the pcDNA3.1+N-eGFP-FLCNΔE510 vectors were generated by Genscript ...
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bioRxiv - Molecular Biology 2020Quote: ... USP7 was expressed with an N-terminal myc-tag from pcDNA3.1 (Genscript). All mutations were generated by Genscript ...
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bioRxiv - Molecular Biology 2020Quote: Full-length wild-type human FLCN cDNA carrying an N-terminal RGS6xHis-tag was expressed from pcDNA3.1 (Genscript). USP7 was expressed with an N-terminal myc-tag from pcDNA3.1 (Genscript) ...
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bioRxiv - Neuroscience 2020Quote: ... and the supernatant was loaded onto a pre-equilibrated column of anti-DYKDDDDK G1 affinity resin (Genscript). The resin was washed with Buffer A (20 mM HEPES ...
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bioRxiv - Zoology 2020Quote: ... and GLUT5 (MT472840) were synthesized by GenScript based on the full-length mRNA sequences derived from our previously published RNA sequencing data (Workman et al. ...
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bioRxiv - Bioengineering 2020Quote: ... The EQCi plasmids for multiplex gene repression (harboring multiple sgRNA cassettes) were constructed by GenScript (Nanjing, China).
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bioRxiv - Bioengineering 2020Quote: The codon optimized thermophilic lacZ gene (32) under the control of the GBL-responsive promoter srbAp or the strong constitutive promoter and ermEp* (GenScript, Nanjing, China) was cloned in the integrative vector pSET152 between NdeI and XbaI to yield the plasmids of pSET-srbAp-lacZ and pSET-ermEp*-lacZ ...
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bioRxiv - Biochemistry 2020Quote: The yeast Rev1 construct was purchased from GenScript. All Rev1 proteins were expressed in BL21(DE3 ...
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bioRxiv - Immunology 2020Quote: ... synthesized de novo (Genscript), then cloned into pcDNA3.1(+ ...
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bioRxiv - Biophysics 2020Quote: The plasmid containing the catalytic domain of PKA (PKAc) was a gift from Susan Taylor via Addgene (#14921).13 The other plasmids were synthesized by Genscript and codon optimized for expression in E ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-2 nsp7 gene (nucleotide 11846-12091, GenBank: MN908947.3) was synthesized de novo by GenScript (Nanjing, China) and cloned into the pMal-c5X vector using the same way as nsp8 ...
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bioRxiv - Biochemistry 2020Quote: SARS-CoV-2 nsp8 gene (nucleotide 12092-12685, strain, GenBank: MN908947.3) was synthesized de novo by GenScript (Nanjing, China) and cloned into a pMal-c5X vector under tac promoter control (without a maltose-binding protein sequence) ...
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bioRxiv - Biochemistry 2020Quote: SARS-CoV-2 nsp12 gene (amino acid 4393-5324 Uniprot: P0DTD1) was synthesized de novo by GenScript (Nanjing, China) and constructed onto pET22b vector between NdeI and XhoI sites ...
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bioRxiv - Biochemistry 2020Quote: ... were chemically synthesized and purified by HPLC by Genscript (Nanjing, China). Stellar competent cells were purchased from Takara (Mountain ...
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bioRxiv - Synthetic Biology 2020Quote: ... A synthetic promoter (PLexA) consisting of eight repeats of the LexA operator and a minimal GAL1 promoter was also synthesised (GenScript). LexA-DAD2 ...
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bioRxiv - Biophysics 2020Quote: The mouse 5-HT3AR (NCBI Reference Sequence: NM_001099644.1) gene was codon-optimized for Spodoptera frugiperda (Sf9) cells and purchased from GenScript. The construct consists of the 5-HT3AR gene along with a C-terminal 1D4-tag42 and four strep-tags (WSHPQFEK ...
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bioRxiv - Biophysics 2020Quote: Mouse 5-HT3AR gene (purchased from GenScript) and mutant genes were inserted into pTLN plasmid ...