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5001 - 5050 of 6194 citations since 2019

• Distinct conformational states of SARS-CoV-2 spike protein

  Yongfei Cai, et al., bioRxiv - Biochemistry 2020

  Quote: ... of SARS-CoV-2 and the ectodomain of human angiotensin converting enzyme 2 (ACE2; residue 1-615) were synthesized by GenScript (Piscataway, NJ). The S gene was fused with a C-terminal twin Strep tag [(GGGGS)2WSHPQFEK(GGGGS)2WSHPQFEK)] and cloned into a mammalian cell expression vector pCMV-IRES-puro (Codex BioSolutions ...
• How to navigate the myosin-V motor through the actin network

  Peter Spieler, et al., bioRxiv - Biochemistry 2020

  Quote: ... A number of constructs were codon-optimized for expression in insect cells and synthesized commercially (GenScript, Piscataway, U.S.A.). DrRab27a ...
• Single-chain lanthanide luminescence biosensors for cell-based imaging and screening of protein-protein interactions

  Ting Chen, et al., bioRxiv - Biochemistry 2020

  Quote: A 630 bp (ER/K)30nm linker fragment (sequence reported in Sivaramakdrishnan and Spudich30) was synthesized and cloned into pUC57 vector by GenScript, Inc ...
• Structural Basis for Ca²⁺-Dependent Activation of a Plant Metacaspase

  Ping Zhu, et al., bioRxiv - Biochemistry 2020

  Quote: ... Extracted proteins were separated in 4-20% precast gradient PAGE gels (Genscript) and transferred to PVDF membranes for immunoblot ...
• Structural Basis for Ca²⁺-Dependent Activation of a Plant Metacaspase

  Ping Zhu, et al., bioRxiv - Biochemistry 2020

  Quote: ... Proteins were separated in 4-20% gradient precast PAGE gels (Genscript) and stained by Coomassie blue.
• Comparative characterisation of Plasmodium falciparum Hsp70-1 relative to E. coli DnaK reveals functional specificity of the parasite chaperone

  Charity Mekgwa Lebepe, et al., bioRxiv - Biochemistry 2020

  Quote: ... The DNA segment encoding PfHsp40 was produced by GenScript (USA) and integrity of the resultant pQE30/PfHsp40 was confirmed by agarose gel electrophoresis and DNA sequencing ...
• The E3 ubiquitin-protein ligase MDM2 is a novel interactor of the von Hippel-Lindau tumor suppressor

  Antonella Falconieri, et al., bioRxiv - Biochemistry 2020

  Quote: ... Genes encoding the human MDM2 and pVHL30 proteins were obtained from commercial plasmid provided by GenScript (GenEZ plasmid OHu28568 and OHu23297) and cDNA transferred into pGBKT7 and pGADT7 plasmids (Clontech ...
• Exploration of natural red-shifted rhodopsins using a machine learning-based Bayesian experimental design

  Keiichi Inoue, et al., bioRxiv - Biochemistry 2020

  Quote: ... coli (Genscript, NJ) were incorporated into the multi-cloning site in the pET21a(+ ...
• Molecular basis for the distinct cellular functions of the Lsm1-7 and Lsm2-8 complexes

  Eric J. Montemayor, et al., bioRxiv - Biochemistry 2020

  Quote: A codon optimized open reading frame (Genscript) for S ...
• Molecular basis for the distinct cellular functions of the Lsm1-7 and Lsm2-8 complexes

  Eric J. Montemayor, et al., bioRxiv - Biochemistry 2020

  Quote: ... Soluble protein was further purified via glutathione agarose chromatography (GenScript # L00206) with gravity flow at room temperature in fresh buffer B ...
• Molecular basis for the distinct cellular functions of the Lsm1-7 and Lsm2-8 complexes

  Eric J. Montemayor, et al., bioRxiv - Biochemistry 2020

  Quote: Codon optimized open reading frames (Genscript) for S ...
• Development of a continuous fluorescence-based assay for N-terminal acetyltransferase D

  Yi-Hsun Ho, Lan Chen, Rong Huang, bioRxiv - Biochemistry 2020

  Quote: ... The plasmid encoded an N-terminal 8X His-SUMO-tagged hNatD in the pET-21a vector was obtained from Genscript. Library of Pharmaceutically Active Compounds (LOPAC ...
• Development of a continuous fluorescence-based assay for N-terminal acetyltransferase D

  Yi-Hsun Ho, Lan Chen, Rong Huang, bioRxiv - Biochemistry 2020

  Quote: The full-length human NatD gene was amplified and cloned into a modified pET-21a(+) vector containing an 8x-His-tag and SUMO protease cleavage site at the N-terminus (Genscript). This pET-21a(+)-hNatD construct was transformed into Escherichia coli BL21 (DE3 ...
• A nucleotide-sensing endonuclease from the Gabija bacterial defense system

  Rui Cheng, et al., bioRxiv - Biochemistry 2020

  Quote: Oligonucleotides and primers were obtained from Genscript Company ...
• Molecular insights into substrate recognition and discrimination by the N-terminal domain of Lon AAA+ protease

  Shiou-Ru Tzeng, et al., bioRxiv - Biochemistry 2020

  Quote: Sul20 peptide (Sequence: ASSHATRQLSGLKIHSNLYH) was synthesized by GenScript (https://www.genscript.com/) at >95% purity.
• Structural properties and peptide ligand binding of the capsid homology domains of human Arc

  Erik I. Hallin, Clive R. Bramham, Petri Kursula, bioRxiv - Biochemistry 2020

  Quote: Peptides were synthesised by GenScript (Piscataway NJ, USA), carrying N-terminal acetylation and C-terminal amidation ...
• Precision Targeting of the Plasminogen Activator Inhibitor-1 Mechanism Increases Efficacy of Fibrinolytic Therapy in Empyema

  Galina Florova, et al., bioRxiv - Biochemistry 2020

  Quote: DSP (EEIIMD) was synthesized by GenScript.
• Histidine-rich Ca²⁺-binding protein stimulates the transport cycle of SERCA through a conformation-dependent fuzzy complex

  Temitope I. Ayeotan, et al., bioRxiv - Biochemistry 2020

  Quote: Expression constructs for the following variants of the SERCA binding region of rabbit HRC (Uniprot: P16230-1) were purchased from Genscript: 496-656 ...
• Ebselen, disulfiram, carmofur, PX-12, tideglusib, and shikonin are non-specific promiscuous SARS-CoV-2 main protease inhibitors

  Chunlong Ma, et al., bioRxiv - Biochemistry 2020

  Quote: ... coli codon optimization was ordered from GenScript in the pET28b(+ ...
• Ebselen, disulfiram, carmofur, PX-12, tideglusib, and shikonin are non-specific promiscuous SARS-CoV-2 main protease inhibitors

  Chunlong Ma, et al., bioRxiv - Biochemistry 2020

  Quote: ... coli codon optimization was ordered from GenScript in the pET28b(+ ...
• Ebselen, disulfiram, carmofur, PX-12, tideglusib, and shikonin are non-specific promiscuous SARS-CoV-2 main protease inhibitors

  Chunlong Ma, et al., bioRxiv - Biochemistry 2020

  Quote: ... coli codon optimization was ordered from GenScript in the pET28b(+ ...
• Ebselen, disulfiram, carmofur, PX-12, tideglusib, and shikonin are non-specific promiscuous SARS-CoV-2 main protease inhibitors

  Chunlong Ma, et al., bioRxiv - Biochemistry 2020

  Quote: ... coli codon optimization was ordered from GenScript in the pET28b(+ ...
• Ebselen, disulfiram, carmofur, PX-12, tideglusib, and shikonin are non-specific promiscuous SARS-CoV-2 main protease inhibitors

  Chunlong Ma, et al., bioRxiv - Biochemistry 2020

  Quote: ... coli codon optimization was ordered from GenScript (Piscataway, NJ). The expression and purification of SARS-CoV-2 Mpro was described as previously.11
• Ebselen, disulfiram, carmofur, PX-12, tideglusib, and shikonin are non-specific promiscuous SARS-CoV-2 main protease inhibitors

  Chunlong Ma, et al., bioRxiv - Biochemistry 2020

  Quote: ... coli codon optimization was ordered from GenScript in the pET28b(+ ...
• Mapping the substrate sequence and length of the Plasmodium M1 and M17 aminopeptidases

  Tess R Malcolm, et al., bioRxiv - Biochemistry 2020

  Quote: Genes encoding Pb-M1 (PlasmoDB ID: PBANKA_1410300, UniProt ID: A0A509ATR4) and Pb-M17 (PlasmoDB ID: PBANKA_1309900, UniProt ID A0A509APA2) were purchased from GenScript. The synthesized genes were codon optimized for gene expression in Escherichia coli ...
• Metal Dependent Dynamic Equilibrium: A Regulatory Mechanism for M17 Aminopeptidases from Plasmodium falciparum and Plasmodium vivax

  Tess R. Malcolm, et al., bioRxiv - Biochemistry 2020

  Quote: ... The PfA-M17 protein was produced from constructs described previously (11) and mutants PfA-M17(AL) and PfA-M17(W525A + Y533A) were synthesized by GenScript. Wild type and mutant sequences were confirmed using Sanger sequencing.
• Mass spectrometric based detection of protein nucleotidylation in the RNA polymerase of SARS-CoV-2

  Brian J. Conti, et al., bioRxiv - Biochemistry 2020

  Quote: ... 1-932 and EAV nsp9 1-693 was synthesized with codon optimization (Genscript) and cloned into pFastBac with an N-terminal MG addition and C-terminal TEV protease site and two Strep tags ...
• Structural impact on SARS-CoV-2 spike protein by D614G substitution

  Jun Zhang, et al., bioRxiv - Biochemistry 2020

  Quote: ... of SARS-CoV-2 (D614) and the ectodomain of human angiotensin converting enzyme 2 (ACE2; residue 1-615) were synthesized by GenScript (Piscataway, NJ). The S gene was fused with a C-terminal twin Strep tag [(GGGGS)2WSHPQFEK(GGGGS)2WSHPQFEK)] and cloned into a mammalian cell expression vector pCMV-IRES-puro (Codex BioSolutions ...
• Proteomic characterization of pilot scale hot-water extracts from the industrial carrageenan red seaweed Eucheuma denticulatum

  Simon Gregersen, et al., bioRxiv - Biochemistry 2020

  Quote: ... SDS-PAGE analysis was performed on precast 4-20% gradient gels (GenScript, USA) in a Tris-MOPS buffered system under reducing conditions according to manufacturer guidelines ...
• A temperature-sensitive Mycobacterium smegmatis glgE mutation leads to a loss of GlgE enzyme activity and thermostability and the accumulation of α-maltose-1-phosphate

  Karl Syson, et al., bioRxiv - Biochemistry 2020

  Quote: ... smegmatis glgE gene was synthesized with optimized codon usage for expression in Escherichia coli (Genscript Corporation, Piscataway, NJ), allowing the production of GlgE with a 6×His tag and a TEV cleavage site at its N-terminus ...
• Experimental and computational studies on molecular mechanism by which Curcumin allosterically inhibits Dengue protease

  Liangzhong Lim, et al., bioRxiv - Biochemistry 2020

  Quote: ... which was purchased from GenScript (Piscataway, NJ), while HPLC-purified Curcumin was from Sigma Aldrich with the purity >93% ...
• Indirect traumatic optic neuropathy after head trauma in adolescent male mice is associated with behavioral visual impairment, neurodegeneration, and elevated endoplasmic reticulum stress markers at acute and subacute times

  Shelby M. Cansler, et al., bioRxiv - Neuroscience 2020

  Quote: ... 40μl of protein sample was loaded into each well and run at 200V for roughly 1.5 hours in Tris-MOPS-SDS running buffer (GenScript; cat # M00138). Gels were then transferred via a wet transfer using Transfer Buffer Powder in 1xTBST (GenScript ...
• Indirect traumatic optic neuropathy after head trauma in adolescent male mice is associated with behavioral visual impairment, neurodegeneration, and elevated endoplasmic reticulum stress markers at acute and subacute times

  Shelby M. Cansler, et al., bioRxiv - Neuroscience 2020

  Quote: ... Gels were then transferred via a wet transfer using Transfer Buffer Powder in 1xTBST (GenScript; cat # M00139) for 1 hour at 30V onto Amersham Hybond P 0.45 PVDF membranes (GE Life Sciences ...
• Indirect traumatic optic neuropathy after head trauma in adolescent male mice is associated with behavioral visual impairment, neurodegeneration, and elevated endoplasmic reticulum stress markers at acute and subacute times

  Shelby M. Cansler, et al., bioRxiv - Neuroscience 2020

  Quote: ... Using SurePAGE 4-12% bis-tris gels (GenScript, Piscataway, NJ; cat # M00653), 40μl of protein sample was loaded into each well and run at 200V for roughly 1.5 hours in Tris-MOPS-SDS running buffer (GenScript ...
• Structure-Guided Mutagenesis Alters Deubiquitinating Activity and Attenuates Pathogenesis of a Murine Coronavirus

  Xufang Deng, et al., bioRxiv - Microbiology 2020

  Quote: ... synthesized by Genscript (Piscataway, NJ) (sequence available upon request) ...
• CELL ATLAS OF THE CHICK RETINA: SINGLE CELL PROFILING IDENTIFIES 136 CELL TYPES

  Masahito Yamagata, Wenjun Yan, Joshua R. Sanes, bioRxiv - Neuroscience 2020

  Quote: ... The crRNA (0.1nmole) was first annealed with an equimolar amount of transactivating crRNA (tracrRNA) in 5 µl in the annealing buffer (GenScript) by heating at 95°C for 5 min followed by rapid chilling ...
• De novo 3D models of SARS-CoV-2 RNA elements and small-molecule-binding RNAs to aid drug discovery

  Ramya Rangan, et al., bioRxiv - Biochemistry 2020

  Quote: ... A DNA library for these constructs was synthesized by Genscript, with each construct 127 bases including the Phi2.5 T7 RNA polymerase promoter sequence (26 ...
• Aggregation and immobilisation of membrane proteins interplay with local lipid order in the plasma membrane of T cells

  Iztok Urbančič, et al., bioRxiv - Biophysics 2020

  Quote: ... whereas the alpha and beta chains for pMHC (HLA-A) were purified from inclusion bodies and folded in the presence of gp100 (agonist; YLEPGPVTV; GenScript). UCHT-1VH-CD45R0-C-tag/His tag vector was co-expressed along with UCHT-1VL transiently in HEK 293T cells ...
• Production of the infant formula ingredient 1,3-olein-2-palmitin in Arabidopsis seeds

  Harrie van Erp, et al., bioRxiv - Bioengineering 2020

  Quote: ... synthesised by Genscript and supplied in pUC57 ...
• RIPK2 Stabilizes c-Myc and is an Actionable Target for Inhibiting Prostate Cancer Metastasis

  Yiwu Yan, et al., bioRxiv - Cancer Biology 2020

  Quote: ... and a MKK7 vector (GenScript, Ohu28940), respectively ...
• Tumor neoantigen heterogeneity impacts bystander immune inhibition of pancreatic cancer growth

  Manisit Das, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Murine IL-12 plasmid DNA was procured from Genscript (Piscataway, NJ).
• In vivo inducible reverse genetics in patients’ tumors to identify individual therapeutic targets

  Michela Carlet, et al., bioRxiv - Cancer Biology 2020

  Quote: ... digested the vector with SpeI and SalI and introduced the following elements as a pre-synthetized stretch of DNA (GenScript®, Piscataway, NJ, USA): SpeI - SFFV - lox2272 - mtagBFP (iRFP720 ...
• CRISPR/Cas9-based inactivation of human papillomavirus oncogenes E6 and E7 induces senescence in cervical cancer cells

  Raviteja Inturi, Per Jemth, bioRxiv - Cancer Biology 2020

  Quote: ... The codon modified HPV18 E6 gene was synthesized by GenScript Biotech (Netherlands ...
• Non-canonical open reading frames encode functional proteins essential for cancer cell survival

  John R. Prensner, et al., bioRxiv - Cancer Biology 2020

  Quote: ... Constructs were generated via commercial gene synthesis (GenScript, Piscataway, NJ).
• Non-canonical open reading frames encode functional proteins essential for cancer cell survival

  John R. Prensner, et al., bioRxiv - Cancer Biology 2020

  Quote: The ORFeome library was then generated via insert synthesis and cloning of unique plasmid inserts consisting unique barcodes (Supplementary Table 22) by a commercial vendor (GenScript, Piscataway, NJ) in arrayed barcoded tube format ...
• Non-canonical open reading frames encode functional proteins essential for cancer cell survival

  John R. Prensner, et al., bioRxiv - Cancer Biology 2020

  Quote: ... sgRNAs were obtained from the Broad Institute Genomic Perturbation Platform (Broad Institute, Cambridge, MA, USA) or from direct synthesis into the BRDN0003 backbone via commercial vendor (GenScript, Piscataway, NJ). sgRNA sequences are listed below:
• Homologous chromosomes in asexual rotifer Adineta vaga suggest automixis

  Paul Simion, et al., bioRxiv - Evolutionary Biology 2020

  Quote: ... We ordered 6 libraries from GenScript® ...
• Granulocyte-Colony Stimulating Factor reduces cocaine-seeking and downregulates glutamatergic synaptic proteins in medial prefrontal cortex

  Rebecca S. Hofford, et al., bioRxiv - Neuroscience 2020

  Quote: ... Rat granulocyte colony stimulating factor (G-CSF) was purchased from GenScript Corp (Piscataway NJ ...
• Intestine-specific deletion of metal transporter Zip14 (Slc39a14) causes brain manganese overload and locomotor defects of manganism

  Tolunay B. Aydemir, et al., bioRxiv - Neuroscience 2020

  Quote: ... Rabbit anti-mouse ZIP14 antibody was custom made by Genscript. Antibodies for GAPDH and actin were obtained from Cell Signalling.
• Molecular Diversity Among Adult Human Hippocampal and Entorhinal Cells

  Daniel Franjic, et al., bioRxiv - Neuroscience 2020

  Quote: ... Coli ArcticExpress and purified from inclusion bodies by GenScript. Recombinant RTN3 ...