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Citations for Takara Bio :
1 - 50 of 2404 citations for Water PCR Grade since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... washed with 200 µL water, and resuspended in PCR mix (22 µL water, 25 µL Terra PCR direct buffer, 1 µL Terra Polymerase (Takara), 1 µL 100 M Truseq PCR handle primer ...
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bioRxiv - Cancer Biology 2023Quote: ... RT-PCR was performed using diluted cDNA (1:5 in water) and PrimeStar DNA Polymerase (Takara, R010A) with primers and PCR conditions listed in Table 1 ...
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bioRxiv - Developmental Biology 2019Quote: ... The cDNA was diluted 1:1 with nuclease free water and subjected to qRT-PCR using SyBr green mix (Takara) in real-time PCR machine (BioRad CFX96) ...
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bioRxiv - Developmental Biology 2021Quote: ... 350 ng RNA samples in 6 µL nuclease-free water) were generated using the Clontech SMARTer smRNA-Seq kit using 8 PCR cycles (Takara Bio). 30 µL of the PCR reaction was purified with AMPure XP beads (Beckman Coulter A63880 ...
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bioRxiv - Systems Biology 2019Quote: ... and RNase-free water (TaKaRa, Japan)) in a 96-well PCR plate (BIOplastics).
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bioRxiv - Bioengineering 2021Quote: ... 7 μL RNase-free water (Takara, Japan), and 10 μL iQ SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2019Quote: ... and 8 μL of RNase-free water (Takara). The experiment of each sample was performed more than three times on a newly prepared printing chip and PCR substrate ...
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bioRxiv - Bioengineering 2023Quote: ... RNA was resuspended in nuclease-free water (TAKARA) and then treated with DNAse using the TURBO DNA-free Kit (Invitrogen).
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bioRxiv - Cancer Biology 2023Quote: ... water-soluble tetrazolium salt (WST-1) (Takara, India). The western diet was customized and purchased from Research Diets Inc ...
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bioRxiv - Microbiology 2024Quote: ... 19.5 µl of nuclease-free water (Takara Bio), and 5 µl of the DNA template ...
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bioRxiv - Cell Biology 2020Quote: ... HBO can be cryopreserved by using STEM-CELLBANKER GMP grade (TaKaRa Bio).
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bioRxiv - Developmental Biology 2023Quote: ... The constructed plasmids then were purified using nucleospin plasmid-transfection grade (TAKARA) and transfected into piezo1-deficient N2A cells (Sugisawa et al. ...
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bioRxiv - Neuroscience 2022Quote: ... were amplified and purified by Nucleospin plasmid TF grade (Takara bio, Shiga, Japan). All plasmid sequences were verified using Sanger sequencing (3100 Genetic Analyzer ...
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bioRxiv - Microbiology 2021Quote: ... recombined by PCR (TaKaRa PCR kit (Clontech Laboratories)) using the primers and cycle conditions in Table S1C ...
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bioRxiv - Microbiology 2021Quote: ... recombined by PCR (TaKaRa PCR kit (Clontech Laboratories)) using the primers and cycle conditions in Table S1C ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR (CloneAmp HiFi PCR Premix, Takara-Bio Europe) genotyping was done with primers designed for each knock-out line that allowed for confirming either the presence of the wild-type (F-gt5/R-gt5WT and F-gt3WT/R-gt3 ...
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bioRxiv - Plant Biology 2023Quote: ... we PCR amplified (CloneAMP HIFI PCR premix, TAKARA) the coding sequences of ATG8a ...
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bioRxiv - Molecular Biology 2021Quote: ... Beads are then subjected to three TE-TW washes and loaded into WTA PCR (100 μM Truseq PCR primer: IDT, CTACGACGCTCTTCCGATCT; 100 μM SMART PCR primer: IDT, AAGCAGTGGTATCAACGCAGAGT; Terra PCR mix: Takara Bio, 639284) with cycling conditions 98°C for 2min ...
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bioRxiv - Molecular Biology 2020Quote: Primers (PAGE grade, fasmac, Appendix Table S4) were labelled with 32P-γ-ATP by T4PNK (Takara), followed by filtering through MicroSpin G-25 Columns (GE healthcare) ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR was done using the Terra™ PCR Direct PCR mix (Takara Bio, Kusatsu, Shiga Japan). Genotyping for Lhx2 was performed as described earlier (Mangale et al. ...
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bioRxiv - Cancer Biology 2023Quote: For indexing PCR, 78μL PCR mix (24μL H20, 50μL 2X SeqAmp CB PCR buffer (Takara Cat. #638526), 2μL SeqAmp DNA polymerase ((Takara Cat ...
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bioRxiv - Cell Biology 2021Quote: ... PCR was performed using SaphireAmp Fast PCR Master Mix (Takara) in 50 μl volume.
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bioRxiv - Biochemistry 2023Quote: ... All PCR was done with HiFi PCR master mix (Takara) following the manufacturer recommendations.
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bioRxiv - Microbiology 2022Quote: All plasmid inserts were amplified by PCR using standard PCR conditions and the CloneAmp HiFi PCR premix (Takara). Following a PCR purification step (QIAquick PCR purification kit) ...
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bioRxiv - Cell Biology 2020Quote: ... nested PCR was performed using PCR Mycoplasma Detection Set (Takara, 6601).
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bioRxiv - Molecular Biology 2021Quote: ... PCR reactions were performed using CloneAmp™ HiFi PCR Premix (Takara) for 35 cycles with an annealing temperature of 52 °C according to the manufacturer’s suggestions ...
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bioRxiv - Immunology 2021Quote: ... with the Power PCR TB green PCR master mix (Takara, Japan). 2µl of sample cDNA was used in a total volume of 10µl (3µl primer mix and 5µl of TB green ...
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bioRxiv - Microbiology 2019Quote: PCR were performed using the Terra PCR Direct Polymerase Mix (Clontech) directly on the cell lysates using the following primers:
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bioRxiv - Microbiology 2019Quote: PCR were performed using the Terra PCR Direct Polymerase Mix (Clontech) directly on the cell lysates after each round of co-infection ...
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bioRxiv - Biochemistry 2019Quote: ... The PCR products were extracted with PCR purification kits (Takara 9761) and ligated using a Blunting Kination Ligation Kit (Takara 6217) ...
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bioRxiv - Genomics 2020Quote: ... cDNA was PCR-amplified using Terra PCR Direct Polymerase (Takara Bio). Final libraries were prepared using 1ng of cDNA per library with the Nextera XT kit (Illumina ...
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bioRxiv - Genetics 2020Quote: ... The PCR was done in PrimeStar GXL PCR reaction (TaKaRa, R050A). The primer sequences are:
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bioRxiv - Immunology 2023Quote: ... PCR was performed on a PCR thermal cycler (Takara, Tokyo, Japan) and real-time PCR was performed using QuantStudio 3 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... Each PCR reaction was composed of CloneAmp HiFi PCR premix (Takara), 4ng of the plasmid template and 10-20ng of the primer pair mix ...
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bioRxiv - Microbiology 2024Quote: ... PCR DNA amplifications were performed with CloneAmp HiFi PCR premix (Takara) using Primer-AP (5’ GACCACGCGTATCGATGTCGAC 3’ ...
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bioRxiv - Plant Biology 2024Quote: ... All PCR reactions were performed using the PCR components from Takara bio Inc ...
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bioRxiv - Developmental Biology 2021Quote: ... PCR templates were generated by PCR reaction with biotinylated primers using high fidelity ClonAmp HiFi PCR mix (Takara Inc.). A mixture of Cas9-mSA mRNA (75ng/μl) ...
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bioRxiv - Developmental Biology 2022Quote: ... PCR templates were generated by PCR reaction with biotinylated primers using high fidelity ClonAmp HiFi PCR mix (Takara Inc.). A mixture of Cas9-mSA mRNA (75ng/ml) ...
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bioRxiv - Molecular Biology 2020Quote: LNA/DNA probes (PAGE grade, fasmac, Table SX) were labelled with 32P-γ-ATP by means T4PNK (Takara), followed to filter by MicroSpin G-25 Columns (GE healthcare) ...
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bioRxiv - Molecular Biology 2020Quote: Primary RT-PCR and secondary PCR was performed using the Primescript III 1-step RT-PCR kit and EmeraldAmp GT PCR Mix (Takara; detailed protocol provided in Supplementary File 1) ...
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bioRxiv - Microbiology 2020Quote: ... All elements of the DiCre plasmid were amplified by PCR using standard PCR conditions (using the CloneAmp HiFi PCR premix) and sequentially ligated (In-Fusion HD Cloning Kit, Clontech) into an acceptor plasmid containing a TgDHFR/TS cassette flanked by two LoxP sites ...
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bioRxiv - Immunology 2023Quote: ... The components of the master mix for the PCR and the PCR programs were set up according to the CloneAmp HIFI PCR protocol (Takara). All generated plasmids were sequenced before use (Eurofins Genomics).
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative PCR (qPCR) was performed on the CFX Connect Real-Time PCR Detection System using SYBR Green PCR Master Mix (TaKaRa). Primer pairs used were listed in Supplementary (Table S2) ...
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bioRxiv - Plant Biology 2024Quote: ... The qRT-PCR analysis was performed using the ABI StepOne Plus PCR system and the SYBR Green Realtime PCR mix (Takara). The ubiquitin gene (Lj5g3v2060710 ...
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bioRxiv - Neuroscience 2021Quote: ... Real time PCR was done via Real-time PCR equipment TP970 (Takara) and analyzed by Thermal Cycler Dice® Real Time System (Takara).
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bioRxiv - Microbiology 2021Quote: ... Quantitative PCR was carried out using SYBR Green Q-PCR kit (Takara).
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bioRxiv - Cell Biology 2021Quote: ... open reading frames were PCR-amplified using CloneAmp HiFi PCR Premix (Clontech), gel-purified using the Nucleospin® Gel and PCR Clean-Up kit (Macherey-Nagel) ...
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bioRxiv - Cell Biology 2021Quote: ... Semi-quantitative PCR was performed with EmeraldAmp MAX PCR Master Mix (Takara). Primers for amlplication of Piezo1 and Gapdh genes were listed in our previous study [26].
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bioRxiv - Microbiology 2021Quote: ... Quantitative PCR was carried out using SYBR Green Q-PCR kit (Takara). Relative expression with respect to control (act2 gene ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR for Gibson assembly was performed using CloneAmp HiFi PCR Premix (Clontech) and Q5 high-fidelity DNA polymerase (NEB) ...