Labshake search
Citations for Takara Bio :
351 - 400 of 2404 citations for Water PCR Grade since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... qRT-PCR was performed by using One Step SYBR® PrimeScript® PLUS RT-RNA PCR Kit (TaKaRa Biotechnology, Dalian, China). Relative expression levels were analyzed by using the 2−ΔΔCT method.
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bioRxiv - Genomics 2020Quote: ... was linearized from the 3’LTR to the RRE by PCR and both PCR products were fused using In-Fusion Cloning (Takara Bio) and transformed into One Shot Stbl3 Chemically Competent E.coli (Life Technologies) ...
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bioRxiv - Microbiology 2020Quote: ... then subjected to quantitative real-time RT-PCR using a One-Step SYBR PrimeScript RT-PCR kit II (Takara Bio, Japan). The PCR mixture was mixed with 2 μl of extracted RNA and DENV-specific primer (38 ...
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bioRxiv - Microbiology 2021Quote: ... PCR products to be used as DNA templates were cleaned using a NucleoSpin Gel and PCR Clean-up kit (Takara Bio). In vitro transcription was performed using MAXIscript T7 Transcription Kit (Thermo Fischer Scientific ...
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bioRxiv - Genomics 2021Quote: ... The four PCR assays were run on a thermal cycler using 100 ng DNA with PrimeStar GXL PCR mastermix (Takara Bio) according to the manufacturer’s instruction using 58 °C annealing temperature ...
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bioRxiv - Microbiology 2022Quote: ... the viral RNA was isolated and amplified by RT-PCR using the PrimeScript II High Fidelity One Step RT-PCR Kit (Takara Bio) and specific primers ...
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bioRxiv - Microbiology 2022Quote: ... The reverse transcription and the first round PCR were performed in a 25μl reaction volume by using PrimeScript™ One-Step RT-PCR Kit (TAKARA, RR055A). Cycling conditions were as follows ...
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Microbiology 2022Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... All PCR purifications were performed using the NucleoSpin® Gel and PCR Clean-up kit (TAKARA Bio Inc., San Jose, CA) according to manufacturer protocols ...
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bioRxiv - Developmental Biology 2023Quote: ... At 48 hpf the GFP + progeny was genotyped using a forward primer in the foxo1a endogenous locus and a reverse primer in the EGFP inserted transgene using a touch-down PCR with the Terra™ PCR Direct Polymerase Mix (639270; Takara). The PCR product was run in a 1% agarose gel to validate its size and the remaining product was sent for Sanger sequence.
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bioRxiv - Microbiology 2023Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using the OneStep TB Green PrimeScript PLUS RT-PCR kit (TAKARA, Cat# RR096A) and the following primers ...
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bioRxiv - Molecular Biology 2023Quote: ... Promoterless bicistronic vectors were generated by removing the SV40 promoter from the bicistronic vector pRUF and the pRUF vectors containing the putative IRES sequences by PCR amplification using divergent primers (Supplementary Table 3) and the CloneAmp™ HiFi PCR Premix (Takara) polymerase ...
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bioRxiv - Genomics 2023Quote: ... A total of 3.5 μl ribo-depleted material was used for SMARTer (SMARTer PCR cDNA Synthesis Kit, catalog num. 634926 and Advantage 2 PCR kit, catalog num. 639207, Clontech-Takara) protocol ...
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bioRxiv - Microbiology 2023Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using a One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Biochemistry 2022Quote: ... cDNAs were generated by PCR amplification in 50 µl-total reaction volume using the Advantage 2 PCR Enzyme System (Clontech, #639206). PCR reaction was performed using 20 µl cDNA from previous step ...
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bioRxiv - Cancer Biology 2022Quote: ... the coding region of RAS cDNA was first PCR-amplified from the plasmids above by using CloneAmp HiFi PCR Premix (Takara #639298) and the primers listed in Supplementary Table 3 using manufacturer’s recommended PCR conditions ...
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bioRxiv - Developmental Biology 2023Quote: ... and 854 bp right homology arm (RHA) were PCR amplified from WTC-mTagRFPT-LMNB1 iPSC genomic DNA with CloneAmp HiFi PCR Premix (Takara #639298). The LHA ...
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bioRxiv - Microbiology 2023Quote: ... 90 μl of RNase free water was added and then 2.5 μl of diluted sample was used for real-time RT-PCR according to the manufacturer’s protocol with One step TB green PrimeScript PLUS RT-PCR Kit (Takara, Cat# RR096A) and primers for Nucleocapsid (N ...
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bioRxiv - Synthetic Biology 2023Quote: ... DNA amplification of phage or bacterial genetic sequences were performed by PCR using the CloneAmp HiFi PCR premix® (Takara©). Primers used in this study are listed in Table S1 ...
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bioRxiv - Microbiology 2023Quote: ... was used as the template for real-time RT-PCR performed according to the manufacturer’s protocol using One Step TB Green PrimeScript PLUS RT-PCR kit (Takara, Cat# RR096A) and the following primers ...
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bioRxiv - Microbiology 2024Quote: ... for genotype analysis was extracted using the Qiagen DNeasy Blood and Tissue kit and PCR analysis was done with CloneAmp™ HiFi PCR Premix (Takara) or Phusion polymerase (NEB).
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bioRxiv - Biophysics 2023Quote: ... 5’-CTGGAGAATCCCGGTGC-3’ and 5’- GTGTCAGATATATACATCCTGT-3’ and the PCR product was purified using a NucleoSpin Gel and PCR Clean-up Maxi kit (Takara Bio). The sequences of the 147 bp and 177 bp DNA are as follows:
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time RT-PCR (qRT-PCR) was performed using TB Green Premix Ex Taq ™ II (Tli RNaseH Plus) (Takara) with qTOWER3 (Jena) ...
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bioRxiv - Genomics 2024Quote: ... The polymerase chain reaction (PCR) was performed on genomic DNA to amplify 16s rRNA using Emerald Amp MAX PCR Master Mix (Takara Bio) using the primers presented in Table S1.
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bioRxiv - Microbiology 2024Quote: ... the LHA and RHA PCR products were used as primers for amplifying the tsp exchange gene using CloneAmp HiFi PCR Premix (Takara Bio). After five PCR cycles ...
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bioRxiv - Microbiology 2024Quote: ... the tsp gene and 500 base pair upstream and downstream of the S117 tsp gene that should be exchanged (Left and Right Homology Arms (LHA and RHA)) were amplified by PCR using CloneAmp HiFi PCR Premix (Takara Bio). LHA and RHA primers carried overhangs with homology to the tsp exchanged gene ...
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bioRxiv - Neuroscience 2020Quote: ... Each cell was then aspirated a final time and deposited into an individual 0.5mL tube containing 9.5ul of nuclease-free water and 1ul of 10x Reaction Buffer (Smart-Seq V4 Ultra Low Input RNA kit, Takara Bio) and allowed to incubate at room temperature for 5 minutes before being stored at −80deg until cDNA synthesis ...
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bioRxiv - Neuroscience 2022Quote: ... prepared freshly with 940 nL of Lysis Buffer 1 (1 μL of 10X reaction buffer is diluted in 2.75 μL of water) (SMART-Seq v4 Ultra Low input RNA kit; Takara Bio Inc.) per well ...
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bioRxiv - Microbiology 2023Quote: ... TwistAmp® Basic Kit (TwistDx, TABAS03KIT), Milenia HybriDtect1 (TwistDX, Milenia01), UltraPure DNase/RNase-Free Distilled Water (Thermo, 10977015) and RNase inhibitor (Takara, 2313A). Thermo Fisher Nanodrop 1000 Spectrophotom was used to quantify nucleic acid ...
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bioRxiv - Immunology 2021Quote: ... The transcripts of the DEMs and DEPs were analyzed and validated by qRT-PCR assays using the Mir-X miRNA qRT-PCR SYBR® Kit (TaKaRa, Japan) and the PrimeScriptTM RT reagent Kit (TaKaRa ...
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bioRxiv - Immunology 2021Quote: ... and the primers of the U6 snRNA used in the qRT-PCR assays were provided in the Mir-X miRNA qRT-PCR SYBR® Kit (Takara, Japan). The qRT-PCR data were analyzed by the 2−ΔΔCt method as described in Livak & Schmittgen (2001 ...
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bioRxiv - Pathology 2020Quote: The viral loads of WHCV in BALF of patient 1 were determined by quantitative real-time RT-PCR with Takara One Step PrimeScript™ RT-PCR Kit (Takara RR064A) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... The PCR products were purified using Nucleo Spin® Gel and PCR Clean-up Kits (Code No. 740609.50; TAKARA Bio, Kusatsu, Japan) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The levels of L6 transcripts observed in semi-Q PCR were further verified through qRT-PCR using SYBR master mix (Takara Bio, USA). Rice act1 was used as a house-keeping gene and the cDNA synthesized from NC was used to normalize the expression pattern by ΔΔCT method (Livak and Schmittgen ...
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bioRxiv - Developmental Biology 2021Quote: ... which then subjected to PCR using Taq Master Mix (Vazyme, p112-01) for RT-PCR or TB Green Premix EX Taq Ⅱ (Takara, RR820A) for quanti tative PCR ...
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bioRxiv - Developmental Biology 2020Quote: ... Hmx1 putative enhancer elements were amplified by PCR from total genomic DNA using the LA Taq PCR kit v2.1 (TaKaRa, Mountain View, CA) and the following primers ...
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bioRxiv - Microbiology 2023Quote: ... levels were measured by a qRT-PCR assay using the One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time) (TaKaRa, Cat# RR096A). The PCR protocol was 42°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... The cells were cultured again overnight and subjected to the quantification of mRNA by qRT-PCR using the CellAmp Direct RNA Prep Kit for RT-PCR (Real Time) (3732, Takara Bio Inc.), One Step TB Green PrimeScript PLUS RT-PCR Kit (Perfect Real Time ...
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bioRxiv - Plant Biology 2023Quote: ... The expression of marker genes was detected by qRT-PCR using the One-step TB Green PrimeScriptTM RT-PCR kit II (Takara, Osaka, Japan). All genes were normalized against the level of an actin reference gene (Foo et al. ...
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bioRxiv - Developmental Biology 2023Quote: ... Quantitative reverse transcriptase PCR (qRT-PCR) was performed using the TB Green Premix Ex Taq II (Takara Bio, San Jose, CA, USA) under the following cycling conditions ...
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bioRxiv - Cell Biology 2024Quote: ... The absence of ATG14 transcripts was verified by Reverse Transcription PCR (RT-PCR) on cDNA synthesized from total RNA extracted from the cell samples using Terra™ PCR Direct Polymerase Mix (TAKARA, 639270). Total RNA was isolated using the NucleoSpin RNA II kit (Macherey-Nagel) ...
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bioRxiv - Molecular Biology 2021Quote: ... RT-PCR was performed using LA Taq (TAKARA Bio). The primer pairs employed for RT-PCR are listed in Supplementary Table 2 ...
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bioRxiv - Developmental Biology 2021Quote: PCRs for were undertaken using ExTaq DNA Polymerase (TaKaRa). Primers used are listed below.
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bioRxiv - Pathology 2022Quote: ... with SYBR Green PCR master mix (Takara, Dalian, China) and gene-specific primers ...
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bioRxiv - Neuroscience 2020Quote: ... a PCR performed using PrimeStar GLX Polymerase (Takara Clontech) with pAAV.Syn.GCaMP6s.WPRE.SV40 (Addgene Plasmid#100843 ...
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bioRxiv - Developmental Biology 2019Quote: ... Probe regions were PCR amplified with Ex Taq (Takara) directly from genomic DNA or cloned plasmid templates to incorporate DIG-labeled dUTP (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... PCR was performed with Advantage cDNA Polymerase (Clontech, 639105) and products were cloned into the pGEM-T Easy vector (Promega ...
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bioRxiv - Molecular Biology 2019Quote: End-point PCR was performed with SapphireAmp (Takara, RR350B) by denaturation at 95°C for 5min ...
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bioRxiv - Plant Biology 2021Quote: ... Procedure for PCR amplification by using ExTaq polymerase (Takara): 94°C 2 min ...