Labshake search
Citations for Takara Bio :
551 - 600 of 773 citations for Vertical Electrophoresis system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... JCaM.1-derived cell lines with tetracycline-inducible gene expression system were maintained in RPMI 1640 supplemented with 10 % tetracycline-negative FBS (Clontech). Cells were routinely tested and found negative for mycoplasma and were not STR profiled.
-
bioRxiv - Microbiology 2021Quote: ... Real-time PCR was performed using the THUNDERBIRD SYBR qPCR Mix (Toyobo) and the Thermal Cycler Dice Real Time System II (Takara). The transcript level in each strain was normalized to the internal control (rnpB) ...
-
bioRxiv - Cancer Biology 2021Quote: ... were generated from pEGFP-C3 K19 WT using the primers listed in Table 1 with the In-Fusion HD cloning system (Takara). GFP and GFP-tagged K19 WT and mutantconstructs were cloned from pEGFP-C3 K19 constructs into pLenti CMV Hygro (plasmid #17484 ...
-
bioRxiv - Cancer Biology 2021Quote: ... 1999)) along with the primers listed in Table 1 and the In-Fusion HD cloning system (Takara, Mountain View, CA) following the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... reverse transcription and cDNA preamplification was performed using the SMARTer Ultra Low RNA Kit for the Fluidigm C1 System (Clontech) according to Fluidigm’s manual for mRNA sequencing on the C1 system ...
-
bioRxiv - Cell Biology 2022Quote: All plasmids construction was performed by PCR amplification (CloneAmp HiFi PCR Premix) using appropriate primers followed by Gibson assembly (In-Fusion HD Cloning system, Clontech) into the EcoRI site of pLVX-puro vector (Clontech ...
-
bioRxiv - Molecular Biology 2020Quote: ... Confirmatory “1-to-1” pairwise assays for selected interactants were performed with the MatchMaker Two-Hybrid System 3 (Clontech Inc.)
-
bioRxiv - Developmental Biology 2019Quote: ... Plasmids pGEX-GST and pGEX-GST-Nogo66 were used to express recombinant proteins using 0.1 mM Isopropyl β-D-1-thiogalactopyranoside induction for 1 h at 37°C in an Escherichia coli BL21 expression system and purified by Glutathione resin (Clontech).
-
bioRxiv - Pathology 2019Quote: ... two independent experiments were performed by mating the aliquoted cells with the Y2HGold strain harboring the bait construct pGBKT7-FgANK1 following instruction of the Matchmaker Gold yeast two-hybrid system (Clontech). Interaction clones were selected on 50 SD/-Trp/-Leu/X-α-Gal/AbA (40 μg/ml X-α-Gal and 200 ng/ml Aureobasidin A ...
-
bioRxiv - Cell Biology 2020Quote: ... Mouse E-Syt3 cDNA was amplified by PCR from a cDNA library constructed with poly(A) RNA from 3T3-L1 adipocytes (Mate & Plate TM Library System, Clontech) and cloned into the PT-Adv vector using 5’and 3’ E-Syt3 primers (Table 1) ...
-
bioRxiv - Genomics 2020Quote: Yeast one-hybridization assay was performed using the Matchmaker® Gold Yeast One-Hybrid System (Clontech, Palo Alto, CA, USA). The promoter sequence (upstream 2kb from the start codon ...
-
bioRxiv - Molecular Biology 2021Quote: ... qRT-PCR was performed using SYBR Premix Ex TaqII (Tli RNaseH Plus) and the Thermal Cycler Dice Real Time System (TaKaRa). The results were analyzed based on standard curves from a serial dilution of cDNA ...
-
bioRxiv - Cancer Biology 2020Quote: ... Human melanoma cell lines and Hema-LP were overlaid with viral particles diluted in DMEM/1x Glutamax with 10% TET System Approved FBS (Clontech) supplemented with 5 μg/ml polybrene (Sigma-Aldrich) ...
-
bioRxiv - Systems Biology 2020Quote: ... The cell lines were cultured in IMDM medium (Quality Biological) supplemented with 10% (v/v) Tet System Approved fetal calf serum (Takara Bio/previously Clontech), 100 U/mL penicillin ...
-
bioRxiv - Plant Biology 2021Quote: ... MEL1 and lacZ) under distinct GAL4 upstream activating sequences as described in Matchmaker GAL4 Two-Hybrid System 3 & Libraries User Manual (Clontech). The transformants were grown on synthetic dropout (SD ...
-
bioRxiv - Plant Biology 2020Quote: The yeast two hybrid (YTH) experiments were performed in three replicates using the Matchmaker Gal4-based two-hybrid system (Clontech) and the Saccharomyces cerevisiae strain Y2HGold as described in (11) ...
-
bioRxiv - Genomics 2019Quote: SiGRF1 protein interactions were investigated by screening a foxtail millet cDNA library in yeast with the Matchmaker Two-Hybrid System 3 (Clontech). SiGRF1 cDNA without the termination codon was cloned with EcoR1 technology in pGBKT7 ...
-
bioRxiv - Cell Biology 2019Quote: ... and lentiviral Tre3G-Cas9 and Tet3G particles were produced in HEK293T cells using the Lenti-X HT packaging system (Clontech). Transduced cells were selected with 10 μg/mL puromycin and 400 μg/mL G418 ...
-
bioRxiv - Neuroscience 2019Quote: ... Israel) into the pEGFP-N2 vector (restriction sites BamHI and NotI, removing EGFP) using the Infusion cloning system from Clontech. The FLAG sequence was then inserted downstream of the signaling peptide (amino acids 1-27 ...
-
bioRxiv - Molecular Biology 2020Quote: PJ69-4a strain of Saccharomyces cerevisiae was used for the expression of proteins of interest using the Matchmaker Gal4-based yeast two hybrid system (Clontech). Tested bait/prey combinations were co-transformed into PJ69-4a and cultivated at 28°C ...
-
bioRxiv - Genomics 2021Quote: ... the target site of the CRISPR/Cas9 system on dnmt3aa gene was amplified using TaKaRa Ex Taq® (Takara Bio) with the primers listed in Supplementary Table S2 ...
-
bioRxiv - Plant Biology 2021Quote: ... was then used for RT-qPCR using the primers shown in Supplementary Table S1 with THUNDERBIRD SYBR qPCR Mix (TOYOBO) on a Thermal Cycler Dice Real Time System (TaKaRa). P ...
-
bioRxiv - Genetics 2021Quote: ... Quantitative PCR was performed using KAPA SYBR Fast qPCR Kits (Nippon Genetics, Japan) on Dice Real Time System Single Thermal Cycler (Takara) or CFX96 Real- Time System (BioRad ...
-
bioRxiv - Molecular Biology 2019Quote: ... The specific PCR products were gel purified by using the DNA Gel Extraction Kit (Axygen, USA) and cloned to the pMD-18 vector system (Takara), and then sequenced by the Shanghai Sangon Company.
-
Osh6 requires Ist2 for localization to the ER-PM contacts and efficient phosphatidylserine transportbioRxiv - Cell Biology 2020Quote: For testing protein-protein interactions Matchmaker™ GAL4 Two-Hybrid System 3 was used according to the manufacturer’s instructions (Clontech). Briefly ...
-
bioRxiv - Plant Biology 2020Quote: ... bait plasmids and prey plasmids were co-transformed into yeast strain AH109 according to the manufacturer’s protocol of GAL4-based yeast two-hybrid system (Clontech, USA). Then ...
-
bioRxiv - Molecular Biology 2021Quote: ... The purified ds CDNA was finally co-transformed with pGAD-T7 Rec into competent Y187 using Yeastmaker yeast transformation system (Takara, Clontech) and plated on SD-Leucine agar media ...
-
bioRxiv - Molecular Biology 2021Quote: ... Recombinant OmpA-pGBKT7 and Flg-pGBKT7 were finally transformed into Y2H Gold yeast strain separately using Yeastmaker yeast transformation system (Takara, Clontech).
-
bioRxiv - Molecular Biology 2021Quote: ... First strand cDNA was synthesized with 3.6 μg of total RNA using Make your own ‘mate & plate’ library system (Takara, Clontech) according to the manufacturer’s instruction ...
-
bioRxiv - Neuroscience 2020Quote: ... qRT-PCR was performed using TOYOBO THUNDERBIRD SYBR qPCR Mix on a Thermal Cycler Dice Real Time System (Takara Bio). The average threshold cycle value (CT ...
-
bioRxiv - Microbiology 2022Quote: ... A tomato Mate & Plate library was constructed and used for screening with the Matchmaker Gold Yeast Two-Hybrid System (Clontech) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: We used the In-Fusion™ (IF) HD cloning system online primer design website (Takara Bio USA, Mountain View, CA) to create primer sets (Table S1 ...
-
bioRxiv - Molecular Biology 2022Quote: ... pCAGGS was digested by SpeI and BamHI and assembled with the PCR products by an In-Fusion HD cloning system (Clontech). After transformation into DH5α ...
-
bioRxiv - Biophysics 2022Quote: ... The cell culture medium was replaced with pre-warmed DMEM containing 10% Tet system approved FBS (Tet-free medium, TaKaRa) and 30 μL of DNA-lipid complex was added to each well ...
-
bioRxiv - Developmental Biology 2022Quote: ... 2017) into the 3’ end of AQP1 in pBS-AQP1 (described above) using the In-Fusion Cloning system (Takara Bio) with the following primers ...
-
bioRxiv - Genomics 2022Quote: Genomic DNA was used as a template for quantitative PCR analysis on a Thermal Cycler Dice Real Time System (TaKaRa) using KAPA SYBR Fast qPCR Kit (KAPA Biosystems ...
-
bioRxiv - Systems Biology 2022Quote: ... with 1 μg of total RNA as input for the SMARTer Apollo NGS library prep system (Takara, Mountain View, CA). The library for RNA-seq was prepared by using NEBNext Ultra II Directional RNA Library Prep kit (New England BioLabs ...
-
bioRxiv - Plant Biology 2022Quote: ... which was conducted with the “Make Your Own “Mate & Plate™” Library System” according to the manufacturer’s instructions with minor modifications (Clontech). Oligo-dT primers provided in the kit were used for first-strand cDNA synthesis with 2 µg total RNA as input ...
-
bioRxiv - Neuroscience 2022Quote: ... These plasmids were obtained by cloning cDNA extracted from mouse cerebellum in pAAV-MCS Expression Vector with In-Fusion Cloning system (Takara). 96h after transfection ...
-
bioRxiv - Plant Biology 2022Quote: ... The SlBZR1 CDS was introduced into the pGBKT7 vector and then used as bait to screen the cDNA library based on the Matchmaker Gold Yeast Two-Hybrid System (Clontech). Yeast transformants were exhaustively selected on SD/-Ade/-His/-Leu/-Trp/X-α-Gal medium ...
-
bioRxiv - Plant Biology 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed on three biological replicates using the Thermal Cycler Dice Real Time System TP-760 (Takara) with the Kapa SYBR fast universal qPCR kit (Kapa Biosystems) ...
-
FTSH3 facilitates complex I degradation through a direct interaction with the complex I subunit PSSTbioRxiv - Plant Biology 2023Quote: ... Mating was carried out in a 96-well plate according to manufacturer’s instructions (Matchmaker GAL4 Two Hybrid System (Clontech/TakaraBio). Mated diploid strains were serially diluted and plated on - Leu–Trp double dropout (DDO ...
-
bioRxiv - Plant Biology 2023Quote: ... pYES2-SiHDA9 and empty pYES2 vector (EV) were transformed individually into Saccharomyces cerevisiae strain W303 using Yeastmaker Yeast Transformation System (Clontech). The transformed cells were screened on SD/-ura medium with 2% (w/v ...
-
bioRxiv - Plant Biology 2023Quote: ... The cDNA library was prepared from rice flowers and ligated to the pGADT7-Rec vector using the Make Your Own “Mate and Plate” Library System (Takara). A second cDNA library was prepared from rice leaves under salt stress ...
-
bioRxiv - Molecular Biology 2022Quote: ... was derived from foreskin-fibroblasts using the Stemgent mRNA Reprogramming Kit (Stemgent) as described previously (40) and maintained in a feeder-free culturing system Cellartis DEF-CS 500 (Takara). The inducible SpCas9 cell line was generated as described earlier (41).
-
bioRxiv - Neuroscience 2023Quote: ... pTRE3G-HA signal-Flag-GPCRs-P2A-mCherry-reverse-PGK-TetOn3G was made of Tet-ON 3G inducible expression system (Clontech) and PRESTO-Tango GPCR Kit (Kroeze et al. ...
-
bioRxiv - Microbiology 2023Quote: A yeast screen for human cellular interacting proteins of pUL136 was performed using the Matchmaker Gold Yeast Two-Hybrid System (Clontech) and the Mate and Plate Universal Human Library (Clontech) ...
-
bioRxiv - Cell Biology 2023Quote: ... HEK293T packaging cells were transfected with the required lentiviral cDNA constructs using Lenti-X packaging system following the manufactures protocol (631276, Takara). SKM cells stably expressing HA-GLUT4-GFP (SKM-GLUT4 cells ...
-
bioRxiv - Neuroscience 2023Quote: Transgene constructs that simultaneously encode FingR targeting PSD95 and membrane markers of neuronal morphology were made using the In-Fusion HD Cloning System (Clontech). First ...
-
bioRxiv - Plant Biology 2023Quote: ... the chimeric H5Indo gene was reamplified by PCR and then introduced in the T-DNA region of a pCAMBIA binary vector previously linearized with restriction enzymes SacII and StuI using the In-Fusion cloning system (Clontech). Expression of H5Indo was driven by a 2X35S promoter from the cauliflower mosaic virus (CaMV) ...